Compositions and method for measuring and calibrating amplification bias in multiplexed PCR reactions

ABSTRACT

Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.14/381,967, filed on Aug. 28, 2014, and issued as U.S. Pat. No.9,150,905 on Oct. 6, 2015, which is a U.S. national phase application ofInternational Patent Application No. PCT/US2013/040221, filed May 8,2013, which claims benefit of U.S. Provisional Application No.61/726,489, filed Nov. 14, 2012 and U.S. Provisional Application No.61/644,294, filed on May 8, 2012, the entire disclosure of which arehereby incorporated by reference in their entirties for all purposes.

DESCRIPTION OF THE TEXT FILE SUBMITTED ELECTRONICALLY

The contents of the text file submitted electronically herewith areincorporated herein by reference in their entirety: A computer readableformat copy of the Sequence Listing (filename:ADBS_001_03US_SeqList_ST25.txt, date recorded: Mar. 25, 2015, file size4.65 MB).

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present disclosure relates generally to quantitative high-throughputsequencing of adaptive immune receptor encoding DNA (e.g., DNA encodingT cell receptors (TCR) and immunoglobulins (IG) in multiplexed nucleicacid amplification reactions. In particular, the compositions andmethods described herein overcome undesirable distortions in thequantification of adaptive immune receptor encoding sequences that canresult from biased over-utilization and/or under-utilization of specificoligonucleotide primers in multiplexed DNA amplification.

2. Description of the Related Art

The adaptive immune system employs several strategies to generate arepertoire of T- and B-cell antigen receptors, i.e., adaptive immunereceptors, with sufficient diversity to recognize the universe ofpotential pathogens. The ability of T cells to recognize the universe ofantigens associated with various cancers or infectious organisms isconferred by its T cell antigen receptor (TCR), which is a heterodimerof an α (alpha) chain from the TCRA locus and β (beta) chain from theTCRB locus, or a heterodimer of a γ (gamma) chain from the TCRG locusand a δ (delta) chain from the TCRD locus. The proteins which make upthese chains are encoded by DNA, which in lymphoid cells employs aunique rearrangement mechanism for generating the tremendous diversityof the TCR. This multi-subunit immune recognition receptor associateswith the CD3 complex and binds to peptides presented by either the majorhistocompatibility complex (MHC) class I or MHC class II proteins on thesurface of antigen-presenting cells (APCs). Binding of TCR to theantigenic peptide on the APC is the central event in T cell activation,which occurs at an immunological synapse at the point of contact betweenthe T cell and the APC.

Each TCR peptide contains variable complementarity determining regions(CDRs), as well as framework regions (FRs) and a constant region. Thesequence diversity of αβT cells is largely determined by the amino acidsequence of the third complementarity-determining region (CDR3) loops ofthe α and β chain variable domains, which diversity is a result ofrecombination between variable (V_(β)), diversity (D_(β)), and joining(J_(β)) gene segments in the β chain locus, and between analogous J_(α)and J_(α) gene segments in the α chain locus, respectively. Theexistence of multiple such gene segments in the TCR α and β chain lociallows for a large number of distinct CDR3 sequences to be encoded. CDR3sequence diversity is further increased by independent addition anddeletion of nucleotides at the V_(β)-D_(β), D_(β)-J_(β), and V_(α)-J_(α)junctions during the process of TCR gene rearrangement. In this respect,immunocompetence is derived from the diversity of TCRs.

The γδ TCR heterodimer is distinctive from the αβTCR in that it encodesa receptor that interacts closely with the innate immune system, andrecognizes antigen in a non-HLA-dependent manner. TCRγδ is expressedearly in development, and has specialized anatomical distribution,unique pathogen and small-molecule specificities, and a broad spectrumof innate and adaptive cellular interactions. A biased pattern of TCRγ Vand J segment expression is established early in ontogeny. Consequently,the diverse TCRγ repertoire in adult tissues is the result of extensiveperipheral expansion following stimulation by environmental exposure topathogens and toxic molecules.

Immunoglobulins (Igs or IG), also referred to herein as B cell receptors(BCR), are proteins expressed by B cells consisting of four polypeptidechains, two heavy chains (H chains) from the IGH locus and two lightchains (L chains) from either the IGK or the IGL locus, forming an H₂L₂structure. H and L chains each contain three complementarity determiningregions (CDR) involved in antigen recognition, as well as frameworkregions and a constant domain, analogous to TCR. The H chains of Igs areinitially expressed as membrane-bound isoforms using either the IGM orIGD constant region exons, but after antigen recognition the constantregion can class-switch to several additional isotypes, including IGG,IGE and IGA. As with TCR, the diversity of naïve Igs within anindividual is mainly determined by the hypervariable complementaritydetermining regions (CDR). Similar to TCRB, the CDR3 domain of H chainsis created by the combinatorial joining of the V_(H), D_(H), and J_(H)gene segments. Hypervariable domain sequence diversity is furtherincreased by independent addition and deletion of nucleotides at theV_(H)-D_(H), D_(H)-J_(H), and V_(H)-J_(H) junctions during the processof Ig gene rearrangement. Distinct from TCR, Ig sequence diversity isfurther augmented by somatic hypermutation (SHM) throughout therearranged IG gene after a naïve B cell initially recognizes an antigen.The process of SHM is not restricted to CDR3, and therefore canintroduce changes to the germline sequence in framework regions, CDR1and CDR2, as well as in the somatically rearranged CDR3.

As the adaptive immune system functions in part by clonal expansion ofcells expressing unique TCRs or BCRs, accurately measuring the changesin total abundance of each T cell or B cell clone is important tounderstanding the dynamics of an adaptive immune response. For instance,a healthy human has a few million unique rearranged TCRβ chains, eachcarried in hundreds to thousands of clonal T-cells, out of the roughlytrillion T cells in a healthy individual. Utilizing advances inhigh-throughput sequencing, a new field of molecular immunology hasrecently emerged to profile the vast TCR and BCR repertoires.Compositions and methods for the sequencing of rearranged adaptiveimmune receptor gene sequences and for adaptive immune receptorclonotype determination are described in U.S. application Ser. No.13/217,126; U.S. application Ser. No. 12/794,507; PCT/US2011/026373; andPCT/US2011/049012, all herein incorporated by reference.

To date, several different strategies have been employed to sequencenucleic acids encoding adaptive immune receptors quantitatively at highthroughput, and these strategies may be distinguished, for example, bythe approach that is used to amplify the CDR3-encoding regions, and bythe choice of sequencing genomic DNA (gDNA) or messenger RNA (mRNA).

Sequencing mRNA is a potentially easier method than sequencing gDNA,because mRNA splicing events remove the intron between J and C segments.This allows for the amplification of adaptive immune receptors (e.g.,TCRs or Igs) having different V regions and J regions using a common 3′PCR primer in the C region. For each TCRβ, for example, the thirteen Jsegments are all less than 60 base pairs (bp) long. Therefore, splicingevents bring identical polynucleotide sequences encoding TCRβ constantregions (regardless of which V and J sequences are used) within lessthan 100 bp of the rearranged VDJ junction. The spliced mRNA can then bereverse transcribed into complementary DNA (cDNA) using poly-dT primerscomplementary to the poly-A tail of the mRNA, random small primers(usually hexamers or nonamers) or C-segment-specific oligonucleotides.This should produce an unbiased library of TCR cDNA (because all cDNAsare primed with the same oligonucleotide, whether poly-dT, randomhexamer, or C segment-specific oligo) that may then be sequenced toobtain information on the V and J segment used in each rearrangement, aswell as the specific sequence of the CDR3. Such sequencing could usesingle, long reads spanning CDR3 (“long read”) technology, or couldinstead involve shotgun assembly of the longer sequences usingfragmented libraries and higher throughput shorter sequence reads.

Efforts to quantify the number of cells in a sample that express aparticular rearranged TCR (or Ig) based on mRNA sequencing are difficultto interpret, however, because each cell potentially expresses differentquantities of TCR mRNA. For example, T cells activated in vitro have10-100 times as much mRNA per cell than quiescent T cells. To date,there is very limited information on the relative amount of TCR mRNA inT cells of different functional states, and therefore quantitation ofmRNA in bulk does not necessarily accurately measure the number of cellscarrying each clonal TCR rearrangement.

Most T cells, on the other hand, have one productively rearranged TCRαand one productively rearranged TCRβ gene (or two rearranged TCRγ andTCRδ), and most B cells have one productively rearranged Ig heavy-chaingene and one productively rearranged Ig light-chain gene (either ICK orIGL) so quantification in a sample of genomic DNA encoding TCRs or BCRsshould directly correlate with, respectively, the number of T or B cellsin the sample. Genomic sequencing of polynucleotides encoding any one ormore of the adaptive immune receptor chains desirably entails amplifyingwith equal efficiency all of the many possible rearranged CDR3 sequencesthat are present in a sample containing DNA from lymphoid cells of asubject, followed by quantitative sequencing, such that a quantitativemeasure of the relative abundance of each rearranged CDR3 clonotype canbe obtained.

Difficulties are encountered with such approaches, however, in thatequal amplification and sequencing efficiencies may not be achievedreadily for each rearranged clone using multiplex PCR. For example, atTCRB each clone employs one of 54 possible germline V region-encodinggenes and one of 13 possible J region-encoding genes. The DNA sequenceof the V and J segments is necessarily diverse, in order to generate adiverse adaptive immune repertoire. This sequence diversity makes itimpossible to design a single, universal primer sequence that willanneal to all V segments (or J segments) with equal affinity, and yieldscomplex DNA samples in which accurate determination of the multipledistinct sequences contained therein is hindered by technicallimitations on the ability to quantify a plurality of molecular speciessimultaneously using multiplexed amplification and high throughputsequencing.

One or more factors can give rise to artifacts that skew the correlationbetween sequencing data outputs and the number of copies of an inputclonotype, compromising the ability to obtain reliable quantitative datafrom sequencing strategies that are based on multiplexed amplificationof a highly diverse collection of TCRβ gene templates. These artifactsoften result from unequal use of diverse primers during the multiplexedamplification step. Such biased utilization of one or moreoligonucleotide primers in a multiplexed reaction that uses diverseamplification templates may arise as a function of differentialannealing kinetics due to one or more of differences in the nucleotidebase composition of templates and/or oligonucleotide primers,differences in template and/or primer length, the particular polymerasethat is used, the amplification reaction temperatures (e.g., annealing,elongation and/or denaturation temperatures), and/or other factors(e.g., Kanagawa, 2003 J. Biosci. Bioeng. 96:317; Day et al., 1996 Hum.Mol. Genet. 5:2039; Ogino et al., 2002 J. Mol. Diagnost. 4:185; Barnardet al., 1998 Biotechniques 25:684; Aird et al., 2011 Genome Biol.12:R18).

Clearly there remains a need for improved compositions and methods thatwill permit accurate quantification of adaptive immune receptor-encodingDNA sequence diversity in complex samples, in a manner that avoidsskewed results such as misleading over- or underrepresentation ofindividual sequences due to biases in the amplification of specifictemplates in an oligonucleotide primer set used for multiplexedamplification of a complex template DNA population. The presentlydescribed embodiments address this need and provide other relatedadvantages.

SUMMARY OF THE INVENTION

A composition for standardizing the amplification efficiency of anoligonucleotide primer set that is capable of amplifying rearrangednucleic acid molecules encoding one or more adaptive immune receptors ina biological sample that comprises rearranged nucleic acid moleculesfrom lymphoid cells of a mammalian subject, each adaptive immunereceptor comprising a variable region and a joining region, thecomposition comprising a plurality of template oligonucleotides having aplurality of oligonucleotide sequences of general formula:5′-U1-B1-V-B2-R-B3-J-B4-U2-3′ [1] wherein: (a) V is a polynucleotidecomprising at least 20 and not more than 1000 contiguous nucleotides ofan adaptive immune receptor variable (V) region encoding gene sequence,or the complement thereof, and each V polynucleotide comprising a uniqueoligonucleotide sequence; (b) J is a polynucleotide comprising at least15 and not more than 600 contiguous nucleotides of an adaptive immunereceptor joining (J) region encoding gene sequence, or the complementthereof, and each J polynucleotide comprising a unique oligonucleotidesequence; (c) U1 is either nothing or comprises an oligonucleotidesequence that is selected from (i) a first universal adaptoroligonucleotide sequence and (ii) a first sequencing platform-specificoligonucleotide sequence that is linked to and positioned 5′ to a firstuniversal adaptor oligonucleotide sequence; (d) U2 is either nothing orcomprises an oligonucleotide sequence that is selected from (i) a seconduniversal adaptor oligonucleotide sequence, and (ii) a second sequencingplatform-specific oligonucleotide sequence that is linked to andpositioned 5′ to a second universal adaptor oligonucleotide sequence;(e) B1, B2, B3, and B4 are each independently either nothing or eachcomprises an oligonucleotide B that comprises a barcode sequence of 3-25contiguous nucleotides, wherein each B1, B2, B3 and B4 comprises anoligonucleotide sequence that uniquely identifies, as a pairedcombination, (i) the unique V oligonucleotide sequence of (a) and (ii)the unique J oligonucleotide sequence of (b);(f) R is either nothing orcomprises a restriction enzyme recognition site that comprises anoligonucleotide sequence that is absent from (a)-(e), and wherein: (g)the plurality of template oligonucleotides comprises at least a or atleast b unique oligonucleotide sequences, whichever is larger, wherein ais the number of unique adaptive immune receptor V region-encoding genesegments in the subject and b is the number of unique adaptive immunereceptor J region-encoding gene segments in the subject, and thecomposition comprises at least one template oligonucleotide for eachunique V polynucleotide and at least one template oligonucleotide foreach unique J polynucleotide.

In one embodiment, a is 1 to a number of maximum V gene segments in themammalian genome of the subject. In another embodiment, b is 1 to anumber of maximum J gene segments in the mammalian genome of thesubject. In other embodiments, a is 1 or b is 1.

In some embodiments, the plurality of template oligonucleotidescomprises at least (a×b) unique oligonucleotide sequences, where a isthe number of unique adaptive immune receptor V region-encoding genesegments in the mammalian subject and b is the number of unique adaptiveimmune receptor J region-encoding gene segments in the mammaliansubject, and the composition comprises at least one templateoligonucleotide for every possible combination of a V region-encodinggene segment and a J region-encoding gene segment. In one embodiment, Jcomprises a constant region of the adaptive immune receptor J regionencoding gene sequence.

In another embodiment, the adaptive immune receptor is selected from thegroup consisting of TCRB, TCRG, TCRA, TCRD, IGH, IGK, and IGL. In someembodiments, the V polynucleotide of (a) encodes a TCRB, TCRG, TCRA,TCRD, IGH, IGK, or IGL receptor V-region polypeptide. In otherembodiments, the J polynucleotide of (b) encodes a TCRB, TCRG, TCRA,TCRD, IGH, IGK, or IGL receptor J-region polypeptide.

In some embodiments, a stop codon is between V and B2.

In one embodiment, each template oligonucleotide in the plurality oftemplate oligonucleotides is present in a substantially equimolaramount. In another embodiment, the plurality of templateoligonucleotides have a plurality of sequences of general formula (I)that is selected from: (1) the plurality of oligonucleotide sequences ofgeneral formula (I) in which the V and J polynucleotides have the TCRB Vand J sequences set forth in at least one set of 68 TCRB V and J SEQ IDNOs. in FIGS. 5a-5l as TCRB V/J set 1, TCRB V/J set 2, TCRB V/J set 3,TCRB V/J set 4, TCRB V/J set 5, TCRB V/J set 6, TCRB V/J set 7, TCRB V/Jset 8, TCRB V/J set 9, TCRB V/J set 10, TCRB V/J set 11, TCRB V/J set 12and TCRB V/J set 13; (2) the plurality of oligonucleotide sequences ofgeneral formula (I) in which the V and J polynucleotides have the TCRG Vand J sequences set forth in at least one set of 14 TCRG V and J SEQ IDNOs. in FIGS. 6a and 6b as TCRG V/J set 1, TCRG V/J set 2, TCRG V/J set3, TCRG V/J set 4 and TCRG V/J set 5; (3) the plurality ofoligonucleotide sequences of general formula (I) in which the V and Jpolynucleotides have the IGH V and J sequences set forth in at least oneset of 127 IGH V and J SEQ ID NOs. in FIGS. 7a-7m as IGH V/J set 1, IGHV/J set 2, IGH V/J set 3, IGH V/J set 4, IGH V/J set 5, IGH V/J set 6,IGH V/J set 7, IGH V/J set 8 and IGH V/J set 9; (4) the plurality ofoligonucleotide sequences of general formula (I) as set forth in SEQ IDNOS:3157-4014; (5) the plurality of oligonucleotide sequences of generalformula (I) as set forth in SEQ ID NOS:4015-4084; (6) the plurality ofoligonucleotide sequences of general formula (I) as set forth in SEQ IDNOS:4085-5200; (7) the plurality of oligonucleotide sequences of generalformula (I) as set forth in SEQ ID NOS:5579-5821; (8) the plurality ofoligonucleotide sequences of general formula (I) as set forth in SEQ IDNOS: 5822-6066; and (9) the plurality of oligonucleotide sequences ofgeneral formula (I) as set forth in SEQ ID NOS: 6067-6191.

In some embodiments, V is a polynucleotide comprising at least 30, 60,90, 120, 150, 180, or 210 contiguous nucleotides of the adaptive immunereceptor V region encoding gene sequence, or the complement thereof. Inanother embodiment, V is a polynucleotide comprising not more than 900,800, 700, 600 or 500 contiguous nucleotides of an adaptive immunereceptor V region encoding gene sequence, or the complement thereof.

In other embodiments, J is a polynucleotide comprising at least 16-30,31-60, 61-90, 91-120, or 120-150 contiguous nucleotides of an adaptiveimmune receptor J region encoding gene sequence, or the complementthereof. In another embodiment, J is a polynucleotide comprising notmore than 500, 400, 300 or 200 contiguous nucleotides of an adaptiveimmune receptor J region encoding gene sequence, or the complementthereof.

In some embodiments, each template oligonucleotide is less than 1000,900, 800, 700, 600, 500, 400, 300 or 200 nucleotides in length.

In other embodiments, the composition includes a set of oligonucleotideprimers that is capable of amplifying rearranged nucleic acid moleculesencoding one or more adaptive immune receptors comprising a plurality a′of unique V-segment oligonucleotide primers and a plurality b′ of uniqueJ-segment oligonucleotide primers. In some embodiments, a′ is 1 to anumber of maximum V gene segments in the mammalian genome, and b′ is 1to a number of maximum number of J gene segments in the mammaliangenome. In one embodiment, a′ is a. In another embodiment, b′ is b.

In yet another embodiment, each V-segment oligonucleotide primer andeach J-segment oligonucleotide primer in the oligonucleotide primer setis capable of specifically hybridizing to at least one templateoligonucleotide in the plurality of template oligonucleotides. In otherembodiments, each V-segment oligonucleotide primer comprises anucleotide sequence of at least 15 contiguous nucleotides that iscomplementary to at least one adaptive immune receptor V region-encodinggene segment. In another embodiment, each J-segment oligonucleotideprimer comprises a nucleotide sequence of at least 15 contiguousnucleotides that is complementary to at least one adaptive immunereceptor J region-encoding gene segment.

In other embodiments, the composition comprises at least one templateoligonucleotide having an oligonucleotide sequence of general formula(I) to which each V-segment oligonucleotide primer can specificallyhybridize, and at least one template oligonucleotide having anoligonucleotide sequence of general formula (I) to which each J-segmentoligonucleotide primer can specifically hybridize.

The invention comprises a method for determining non-uniform nucleicacid amplification potential among members of a set of oligonucleotideprimers that is capable of amplifying rearranged nucleic acid moleculesencoding one or more adaptive immune receptors in a biological samplethat comprises rearranged nucleic acid molecules from lymphoid cells ofa mammalian subject. The method includes steps for: (a) amplifying thecomposition as described herein in a multiplex PCR reaction to obtain aplurality of amplified template oligonucleotides; (b) sequencing saidplurality of amplified template oligonucleotides to determine, for eachunique template oligonucleotide comprising said plurality, (i) atemplate oligonucleotide sequence and (ii) a frequency of occurrence ofsaid template oligonucleotide sequence; and (c) comparing a frequency ofoccurrence of each of said template oligonucleotide sequences to anexpected distribution, wherein said expected distribution is based onpredetermined molar ratios of said plurality of templateoligonucleotides comprising said composition, and wherein a deviationbetween said frequency of occurrence of said template oligonucleotidesequences and said expected distribution indicates a non-uniform nucleicacid amplification potential among members of the set of oligonucleotideamplification primers.

In one embodiment, the predetermined molar ratios are equimolar. Inanother embodiment, the expected distribution comprises a uniformamplification level for said set of template oligonucleotides amplifiedby said set of oligonucleotide primers. In yet another embodiment, eachamplified template nucleic acid molecule is less than 1000, 900, 800,700, 600, 500, 400, 300, 200, 100, 90, 80 or 70 nucleotides in length.

The method includes steps comprising for each member of the set ofoligonucleotide primers that exhibits non-uniform amplificationpotential relative to the expected distribution, adjusting the relativerepresentation of the oligonucleotide primer member in the set ofoligonucleotide amplification primers. In one embodiment, adjustingcomprises increasing the relative representation of the member in theset of oligonucleotide primers, thereby correcting non-uniform nucleicacid amplification potential among members of the set of oligonucleotideprimers. In another embodiment, adjusting comprises decreasing therelative representation of the member in the set of oligonucleotideprimers, thereby correcting non-uniform nucleic acid amplificationpotential among members of the set of oligonucleotide primers.

In other embodiments, the set of oligonucleotide primers does notinclude oligonucleotide primers that specifically hybridize to aV-region pseudogene or orphon or to a J-region pseudogene or orphon.

The method also includes steps comprising: for each member of the set ofoligonucleotide amplification primers that exhibits non-uniformamplification potential relative to the expected distribution,calculating a proportionately increased or decreased frequency ofoccurrence of the amplified template nucleic acid molecules, theamplification of which is promoted by said member, thereby correctingfor non-uniform nucleic acid amplification potential among members ofthe set of oligonucleotide primers.

The invention includes a method for quantifying a plurality ofrearranged nucleic acid molecules encoding one or a plurality ofadaptive immune receptors in a biological sample that comprisesrearranged nucleic acid molecules from lymphoid cells of a mammaliansubject, each adaptive immune receptor comprising a variable (V) regionand a joining (J) region, the method comprising: (A) amplifyingrearranged nucleic acid molecules in a multiplex polymerase chainreaction (PCR) that comprises: (1) rearranged nucleic acid moleculesfrom the biological sample that comprises lymphoid cells of themammalian subject; (2) the composition as described herein wherein aknown number of each of the plurality of template oligonucleotideshaving a unique oligonucleotide sequence is present; (3) anoligonucleotide amplification primer set that is capable of amplifyingrearranged nucleic acid molecules encoding one or a plurality ofadaptive immune receptors from the biological sample.

In some embodiments, the primer set comprises: (a) in substantiallyequimolar amounts, a plurality of V-segment oligonucleotide primers thatare each independently capable of specifically hybridizing to at leastone polynucleotide encoding an adaptive immune receptor V-regionpolypeptide or to the complement thereof, wherein each V-segment primercomprises a nucleotide sequence of at least 15 contiguous nucleotidesthat is complementary to at least one functional adaptive immunereceptor V region-encoding gene segment and wherein the plurality ofV-segment primers specifically hybridize to substantially all functionaladaptive immune receptor V region-encoding gene segments that arepresent in the composition, and (b) in substantially equimolar amounts,a plurality of J-segment oligonucleotide primers that are eachindependently capable of specifically hybridizing to at least onepolynucleotide encoding an adaptive immune receptor J-region polypeptideor to the complement thereof, wherein each J-segment primer comprises anucleotide sequence of at least 15 contiguous nucleotides that iscomplementary to at least one functional adaptive immune receptor Jregion-encoding gene segment and wherein the plurality of J-segmentprimers specifically hybridize to substantially all functional adaptiveimmune receptor J region-encoding gene segments that are present in thecomposition.

In another embodiment, the V-segment and J-segment oligonucleotideprimers are capable of promoting amplification in said multiplexpolymerase chain reaction (PCR) of (i) substantially all templateoligonucleotides in the composition to produce a multiplicity ofamplified template oligonucleotides, said multiplicity of amplifiedtemplate nucleic acid molecules being sufficient to quantify diversityof the template oligonucleotides in the composition, and (ii)substantially all rearranged nucleic acid molecules encoding adaptiveimmune receptors in the biological sample to produce a multiplicity ofamplified rearranged DNA molecules, said multiplicity of amplifiedrearranged nucleic acid molecules being sufficient to quantify diversityof the rearranged nucleic acid molecules in the DNA from the biologicalsample.

In one embodiment, each amplified nucleic acid molecule in the pluralityof amplified template oligonucleotides and in the plurality of amplifiedrearranged nucleic acid molecules is less than 1000 nucleotides inlength; (B) quantitatively sequencing said amplified templateoligonucleotides and said amplified rearranged nucleic acid molecules toquantify (i) a template product number of amplified templateoligonucleotides which contain at least one oligonucleotide barcodesequence, and (ii) a rearranged product number of amplified rearrangednucleic acid molecules which lack an oligonucleotide barcode sequence;(C) calculating an amplification factor by dividing the template productnumber of (B)(i) by the known number of each of the plurality oftemplate oligonucleotides having a unique oligonucleotide sequence of(A)(2); and (D) dividing the rearranged product number of (B)(ii) by theamplification factor calculated in (C) to quantify the number of uniqueadaptive immune receptor encoding rearranged nucleic acid molecules inthe sample.

In other embodiments, the quantified number of unique adaptive immunereceptor encoding rearranged nucleic acid molecules in the sample is thenumber of unique B cell or unique T cell genome templates in the sample.

The invention includes a method for calculating an average amplificationfactor in a multiplex PCR assay, comprising: obtaining a biologicalsample that comprises rearranged nucleic acid molecules from lymphoidcells of a mammalian subject; contacting said sample with a knownquantity of template oligonucleotides comprising a composition asdescribed herein; amplifying the template oligonucleotides and therearranged nucleic acid molecules from lymphoid cells of the mammaliansubject in a multiplex PCR reaction to obtain a plurality of amplifiedtemplate oligonucleotides and a plurality of amplified rearrangednucleic acid molecules; sequencing said plurality of amplified templateoligonucleotides to determine, for each unique template oligonucleotidecomprising said plurality, (i) a template oligonucleotide sequence and(ii) a frequency of occurrence of said template oligonucleotidesequence; and determining an average amplification factor for saidmultiplex PCR reaction based on an average number of copies of saidplurality of amplified template oligonucleotides and said known quantityof said template oligonucleotides.

The method also includes sequencing said plurality of amplifiedrearranged nucleic acid molecules from lymphoid cells of the mammaliansubject to determine for each unique rearranged nucleic acid moleculecomprising said plurality, i) a rearranged nucleic acid moleculesequence and (ii) a number of occurrences of said rearranged nucleicacid molecule sequence; and determining the number of lymphoid cells insaid sample, based on the average amplification factor for saidmultiplex PCR reaction and said number of occurrences of said rearrangednucleic acid molecules.

In other embodiments, the method comprises determining the number oflymphoid cells in said sample comprises generating a sum of the numberof occurrences of each of said amplified rearranged nucleic acidsequences and dividing said sum by said average amplification factor. Insome embodiments, the known quantity is one copy each of said templateoligonucleotides. In one embodiment, 100≦a≦500. In another embodiment,100≦b≦500.

A method is provided for correcting for amplification bias in anmultiplex PCR amplification reaction to quantify rearranged nucleic acidmolecules encoding one or a plurality of adaptive immune receptors in abiological sample that comprises rearranged nucleic acid molecules fromlymphoid cells of a mammalian subject, comprising: (a) contacting saidsample with a composition described herein to generate a template-spikedsample, wherein said templates and said rearranged nucleic acidmolecules comprise corresponding V and J region sequences; (b)amplifying said template-spiked sample in a multiplex PCR reaction toobtain a plurality of amplified template oligonucleotides and aplurality of amplified rearranged nucleic acid molecules encoding aplurality of adaptive immune receptors; (c) sequencing said plurality ofamplified template oligonucleotides to determine, for each uniquetemplate oligonucleotide comprising said plurality, (i) a templateoligonucleotide sequence and (ii) a frequency of occurrence of saidtemplate oligonucleotide sequence; (d) sequencing said plurality ofamplified rearranged nucleic acid molecules encoding one or a pluralityof adaptive immune receptors, for each unique rearranged nucleic acidmolecules encoding said plurality of adaptive immune receptorscomprising said plurality, (i) a rearranged nucleic acid moleculesequence and (ii) a frequency of occurrence of said rearranged nucleicacid molecule sequence; (e) comparing frequency of occurrence of saidtemplate oligonucleotide sequences to an expected distribution, whereinsaid expected distribution is based on predetermined molar ratios ofsaid plurality of template oligonucleotides comprising said composition,and wherein a deviation between said frequency of occurrence of saidtemplate oligonucleotide sequences and said expected distributionindicates non-uniform nucleic acid amplification potential among membersof the set of oligonucleotide amplification primers; (f) generating aset of correction values for a set of template molecules and rearrangednucleic acid molecule sequences amplified by said members of the set ofoligonucleotide amplification primers having said indicated non-uniformnucleic acid amplification potential, wherein said set of correctionvalues corrects for amplification bias in said multiplex PCR reaction;and (g) optionally applying said set of correction values to saidfrequency of occurrence of said rearranged nucleic acid moleculesequences to correct for amplification bias in said multiplex PCRreaction.

The invention comprises a kit, comprising: reagents comprising: acomposition comprising a plurality of template oligonucleotides and aset of oligonucleotide primers as described herein; instructions fordetermining a non-uniform nucleic acid amplification potential amongmembers of the set of oligonucleotide primers that are capable ofamplifying rearranged nucleic acid molecules encoding one or moreadaptive immune receptors in a biological sample that comprisesrearranged nucleic acid molecules from lymphoid cells of a mammaliansubject.

In another embodiment, the kit comprises instructions for correcting forone or more members of the set of oligonucleotide primers having anon-uniform nucleic acid amplification potential.

In other embodiments, the kit comprises instructions for quantifying thenumber of unique adaptive immune receptor encoding rearranged nucleicacid molecules in the sample

These and other aspects of the herein described invention embodimentswill be evident upon reference to the following detailed description andattached drawings. All of the U.S. patents, U.S. patent applicationpublications, U.S. patent applications, foreign patents, foreign patentapplications and non-patent publications referred to in thisspecification and/or listed in the Application Data Sheet areincorporated herein by reference in their entirety, as if each wasincorporated individually. Aspects and embodiments of the invention canbe modified, if necessary, to employ concepts of the various patents,applications and publications to provide yet further embodiments.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

These and other features, aspects, and advantages of the presentinvention will become better understood with regard to the followingdescription, and accompanying drawings, where:

Figure (FIG. 1) shows a schematic diagram of an exemplary templateoligonucleotide for use in standardizing the amplification efficiency ofan oligonucleotide primer set that is capable of amplifying rearrangedDNA encoding an adaptive immune receptor (TCR or BCR). U1, U2, universaladaptor oligonucleotides; B1-4, barcode oligonucleotides; V, variableregion oligonucleotide; J, joining region oligonucleotide; R,restriction enzyme recognition site; S, optional stop codon.

FIG. 2 shows post-amplification frequencies of individual TCRB V genesegment sequences amplified from a standardizing oligonucleotidetemplate composition (an equimolar pool of the templates set forth inSEQ ID NOS:872-1560) using an equimolar (unadjusted) pool of 52 PCRprimers (SEQ ID NOS:1753-1804) and quantitatively sequenced on theIllumina HiSeg™ DNA sequencer. Frequency in the absence of bias wascalculated as 0.0188.

FIG. 3 shows the results of quantitative sequencing followingcross-amplification of template oligonucleotides using TCRB Vregion-specific primers. Y-axis indicates individual amplificationprimers (SEQ ID NOS:1753-1804) that were present in each separateamplification reaction at twice the molar concentration (2×) of theother primers from the same primer set, for amplification of astandardizing oligonucleotide template composition (an equimolar pool ofthe templates set forth in SEQ ID NOS:872-1560); X-axis is not labeledbut data points are presented in the same order as for Y-axis, withX-axis representing corresponding amplified V gene templates asidentified by quantitative sequencing. Black squares indicate no changein degree of amplification with the respective primer present at 2×relative to equimolar concentrations of all other primers; white squaresindicate 10-fold increase in amplification; grey squares indicateintermediate degrees (on a greyscale gradient) of amplification betweenzero and 10-fold. Diagonal line of white squares indicates that 2×concentration for a given primer resulted in about 10-fold increase inamplification of the respective template for most primers. Off-diagonalwhite squares indicate non-corresponding templates to which certainprimers were able to anneal and amplify.

FIG. 4 shows post-amplification frequencies of individual TCRB V genesegment sequences amplified from a standardizing oligonucleotidetemplate composition (an equimolar pool of the templates set forth inSEQ ID NOS:872-1560), using equimolar concentrations of all members of aTCRB amplification primer set (SEQ ID NOS:1753-1804) prior to adjustingfor primer utilization bias (black bars, all V-region primers present inequimolar concentrations), and using the same primer set (SEQ IDNOS:1753-1804) after adjusting multiple individual primer concentrationsto compensate for bias (grey bars, concentrations of highly efficientprimers were reduced and concentrations of poorly efficient primers wereincreased, see Table 6). Post-amplification frequencies were determinedby quantitative sequencing on the Illumina HiSeq™ DNA sequencer.

FIGS. 5a-5l show TCRB V/J sets (68 V+13 J) for use in templatecompositions that comprise a plurality of oligonucleotide sequences ofgeneral formula 5′-U1-B1-V-B2-R-B3-J-B4-U2-3′ [I], for use instandardizing the amplification efficiency of an oligonucleotide primerset that is capable of amplifying rearranged DNA encoding one or aplurality of human T cell receptor β (TCRB) chain polypeptides.

FIGS. 6a and 6b show TCRG V/J sets (14 V+5 J) for use in templatecompositions that comprise a plurality of oligonucleotide sequences ofgeneral formula 5′-U1-B1-V-B2-R-B3-J-B4-U2-3′ [I], for use instandardizing the amplification efficiency of an oligonucleotide primerset that is capable of amplifying rearranged DNA encoding one or aplurality of human T cell receptor γ (TCRG) chain polypeptides.

FIGS. 7a-7m show IGH V/J sets (127 V+9 J) for use in templatecompositions that comprise a plurality of oligonucleotide sequences ofgeneral formula 5′-U1-B1-V-B2-R-B3-J-B4-U2-3′ [I], for use instandardizing the amplification efficiency of an oligonucleotide primerset that is capable of amplifying rearranged DNA encoding one or aplurality of human immunoglobulin heavy (IGH) chain polypeptides.

FIG. 8 shows the results of calculating an amplification factor for eachVJ pair in a template composition that was added to a multiplexed PCRamplification of IGH sequences, and then averaging the amplificationfactor across all synthetic templates to estimate fold sequence coverageacross all synthetic template molecules.

FIG. 9 shows a plot of the numbers of B cells that were estimated usinga synthetic template composition and amplification factor as describedherein, versus the known numbers of B cells used as a source of naturalDNA templates.

FIG. 10 shows a pre-PCR amplification sequencing count for each of 1116IGH VJ bias control molecules and 243 IGH DJ bias control molecules.

FIG. 11 shows TCRB-primer iterations for synthetic TCRB VJ templatesgraphed against relative amplification bias.

FIG. 12 shows IGH primer iterations for synthetic IGH VJ templatesgraphed against relative amplification bias.

FIG. 13 shows the relative amplification bias for 27 synthetic IGH DJtemplates of the V gene.

FIGS. 14a-d show TCRG-primer iterations for 55 synthetic TCRG VJtemplates. Relative amplification bias was determined for the TCRG VJprimers prior to chemical bias control correction (FIG. 14a ), 1stiteration of chemical correction (FIG. 14b ), 2nd iteration of chemicalcorrection (FIG. 14c ), and final iteration of chemical correction (FIG.14d ).

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides, in certain embodiments and as describedherein, compositions and methods that are useful for reliablyquantifying large and structurally diverse populations of rearrangedgenes encoding adaptive immune receptors, such as immunoglobulins (Ig)and/or T cell receptors (TCR). These rearranged genes may be present ina biological sample containing DNA from lymphoid cells of a subject orbiological source, including a human subject.

A “rearranged nucleic acid molecule,” as used herein, can include anygenomic DNA, cDNA, or mRNA obtained directly or indirectly from alymphoid cell line that includes sequences that encode a rearrangedadaptive immune receptor.

Disclosed herein are unexpectedly advantageous approaches for thestandardization and calibration of complex oligonucleotide primer setsthat are used in multiplexed nucleic acid amplification reactions togenerate a population of amplified rearranged DNA molecules from abiological sample containing rearranged genes encoding adaptive immunereceptors, prior to quantitative high throughput sequencing of suchamplified products. Multiplexed amplification and high throughputsequencing of rearranged TCR and BCR (IG) encoding DNA sequences aredescribed, for example, in Robins et al., 2009 Blood 114, 4099; Robinset al., 2010 Sci. Translat. Med. 2:47ra64; Robins et al., 2011 J.Immunol. Meth. doi:10.1016/j.jim.2011.09.001; Sherwood et al. 2011 Sci.Translat. Med. 3:90ra61; U.S. application Ser. No. 13/217,126 (US Pub.No. 2012/0058902), U.S. application Ser. No. 12/794,507 (US Pub. No.2010/0330571), WO/2010/151416, WO/2011/106738 (PCT/US2011/026373),WO2012/027503 (PCT/US2011/049012), U.S. Application No. 61/550,311, andU.S. Application No. 61/569,118; accordingly these disclosures areincorporated by reference and may be adapted for use according to theembodiments described herein.

Briefly and according to non-limiting theory, the present compositionsand methods overcome inaccuracies that may arise in current methodswhich quantify TCR and BCR gene diversity by sequencing the products ofmultiplexed nucleic acid amplification. To accommodate the vastdiversity of TCR and BCR gene template sequences that may be present ina biological sample, oligonucleotide primer sets used in multiplexedamplification reactions typically comprise a wide variety of sequencelengths and nucleotide compositions (e.g., GC content). Consequently,under a given set of amplification reaction conditions, the efficienciesat which different primers anneal to and support amplification of theircognate template sequences may differ markedly, resulting in non-uniformutilization of different primers, which leads to artifactual biases inthe relative quantitative representation of distinct amplificationproducts.

For instance, relative overutilization of some highly efficient primersresults in overrepresentation of certain amplification products, andrelative underutilization of some other low-efficiency primers resultsin underrepresentation of certain other amplification products.Quantitative determination of the relative amount of each templatespecies that is present in the lymphoid cell DNA-containing sample,which is achieved by sequencing the amplification products, may thenyield misleading information with respect to the actual relativerepresentation of distinct template species in the sample prior toamplification. In pilot studies, for example, it was observed thatmultiplexed PCR, using a set of oligonucleotide primers designed to becapable of amplifying a sequence of every possible human TCRB variable(V) region gene from human lymphoid cell DNA templates, did notuniformly amplify TCRB V gene segments. Instead, some V gene segmentswere relatively overamplified (representing ˜10% of total sequences) andother V gene segments were relatively underamplified (representing about4×10⁻³% of total sequences); see also, e.g., FIG. 2.

To overcome the problem of such biased utilization of subpopulations ofamplification primers, the present disclosure provides for the firsttime a template composition and method for standardizing theamplification efficiencies of the members of an oligonucleotide primerset, where the primer set is capable of amplifying rearranged DNAencoding a plurality of adaptive immune receptors (TCR or Ig) in abiological sample that comprises DNA from lymphoid cells. The templatecomposition comprises a plurality of diverse template oligonucleotidesof general formula (I) as described in greater detail herein:5′-U1-B1-V-B2-R-B3-J-B4-U2-3′  (1)

The constituent template oligonucleotides, of which the templatecomposition is comprised, are diverse with respect to the nucleotidesequences of the individual template oligonucleotides. The individualtemplate oligonucleotides thus may vary in nucleotide sequenceconsiderably from one another as a function of significant sequencevariability amongst the large number of possible TCR or BCR variable (V)and joining (J) region polynucleotides. Sequences of individual templateoligonucleotide species may also vary from one another as a function ofsequence differences in U1, U2, B (B1, B2, B3, and B4) and Roligonucleotides that are included in a particular template within thediverse plurality of templates.

In certain embodiments barcode oligonucleotides B (B1, B2, B3, and B4)may independently and optionally comprise an oligonucleotide barcodesequence, wherein the barcode sequence is selected to identify uniquelya particular paired combination of a particular unique V oligonucleotidesequence and a particular unique J oligonucleotide sequence. Therelative positioning of the barcode oligonucleotides B1 and B4 anduniversal adaptors advantageously permits rapid identification andquantification of the amplification products of a given unique templateoligonucleotide by short sequence reads and paired-end sequencing onautomated DNA sequencers (e.g., Illumina HiSeg™ or Illumina MiSEQ®, orGeneAnalyzer™-2, Illumina Corp., San Diego, Calif.). In particular,these and related embodiments permit rapid high-throughput determinationof specific combinations of a V and a J sequence that are present in anamplification product, thereby to characterize the relativeamplification efficiency of each V-specific primer and each J-specificprimer that may be present in a primer set which is capable ofamplifying rearranged TCR or BCR encoding DNA in a sample. Verificationof the identities and/or quantities of the amplification products may beaccomplished by longer sequence reads, optionally including sequencereads that extend to B2.

In use, each template oligonucleotide in the plurality of templateoligonucleotides is present in a substantially equimolar amount, whichin certain preferred embodiments includes preparations in which themolar concentrations of all oligonucleotides are within 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24or 25 percent of each other. In certain other preferred embodiments asprovided herein, template olignucleotides are regarded as being presentin a substantially equimolar amount when the molar concentrations of alloligonucleotides are within one order of magnitude of each other,including preparations in which the greatest molar concentration thatany given unique template oligonucleotide species may have is no morethan 1000, 900, 800, 700, 600, 500, 440, 400, 350, 300, 250, 200, 150,100, 90, 80, 70, 60, 50, 40 or 30 percent greater than the molarconcentration at which is present the unique template oligonucleotidespecies having the lowest concentration in the composition.

In a similar manner, certain embodiments disclosed herein contemplateoligonucleotide primer sets for amplification, in which sets thecomponent primers may be provided in substantially equimolar amounts. Asalso described herein, according to certain other embodiments, theconcentration of one or more primers in a primer set may be adjusteddeliberately so that certain primers are not present in equimolaramounts or in substantially equimolar amounts.

The template composition described herein may, in preferred embodiments,be employed as a nucleic acid amplification (e.g., PCR) template tocharacterize an oligonucleotide primer set, such as the complex sets ofV-segment and J-segment oligonucleotide primers that may be used inmultiplexed amplification of rearranged TCR or Ig genes, for example, aprimer set as provided herein or any of the primer sets described inRobins et al., 2009 Blood 114, 4099; Robins et al., 2010 Sci. Translat.Med. 2:47ra64; Robins et al., 2011 J. Immunol. Meth.doi:10.1016/j.jim.2011.09.001; Sherwood et al. 2011 Sci. Translat. Med.3:90ra61; U.S. application Ser. No. 13/217,126 (US Pub. No.2012/0058902), U.S. application Ser. No. 12/794,507 (US Pub. No.2010/0330571), WO/2010/151416, WO/2011/106738 (PCT/US2011/026373),WO2012/027503 (PCT/US2011/049012), U.S. Application No. 61/550,311, andU.S. Application No. 61/569,118, or the like.

Preferably all templates in the template composition for standardizingamplification efficiency, which is described herein and which comprisesa plurality of template oligonucleotides having diverse sequences andthe general structure of general formula (I), are oligonucleotides ofsubstantially identical length. Without wishing to be bound by theory,it is generally believed that in a nucleic acid amplification reactionsuch as a polymerase chain reaction (PCR), template DNA length caninfluence the amplification efficiency of oligonucleotide primers byaffecting the kinetics of interactions between primers and template DNAmolecules to which the primers anneal by specific, nucleotidesequence-directed hybridization through nucleotide base complementarity.Longer templates are generally regarded as operating less efficientlythan relatively shorter templates. In certain embodiments, the presentlydisclosed template composition for standardizing the amplificationefficiency of an oligonucleotide primer set that is capable ofamplifying rearranged DNA encoding a plurality of TCR or BCR comprises aplurality of template oligonucleotides of general formula (I) asprovided herein, wherein the template oligonucleotides are of anidentical length or a substantially identical length that is not morethan 1000, 950, 900, 850, 800, 750, 700, 650, 600, 550, 500, 450, 400,350, 300, 250, 200, 150 or 100 nucleotides in length, including allinteger values therebetween.

Accordingly, in order to reduce, remove or minimize the potentialcontribution to undesirable biases in oligonucleotide primer utilizationduring multiplexed amplification, preferred embodiments disclosed hereinmay employ a plurality of template oligonucleotides wherein all templateoligonucleotides in the sequence-diverse plurality of templateoligonucleotides are of substantially identical length. A plurality oftemplate oligonucleotides may be of substantially identical length whenall (e.g., 100%) or most (e.g., greater than 50%) such oligonucleotidesin a template composition are oligonucleotides that each have the exactsame number of nucleotides, or where one or more templateoligonucleotides in the template composition may vary in length from oneanother by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,60, 70, 80, 90 or 100 nucleotides in length. It will be appreciated fromthe present disclosure that even in situations where not all templateoligonucleotides have exactly the same length, the herein describedcompositions and methods may still be employed to determine andoptionally correct non-uniform nucleic acid amplification potentialamong members of a set of oligonucleotide amplification primers.

According to certain presently disclosed embodiments, (i) each templateoligonucleotide of the presently described template composition isprovided in a substantially equimolar amount, (ii) the oligonucleotideprimer set that is capable of amplifying rearranged DNA encoding aplurality of adaptive immune receptor comprises a plurality of V-segmentoligonucleotide primers that are provided in substantially equimolaramounts, (iii) the oligonucleotide primer set that is capable ofamplifying rearranged DNA encoding a plurality of adaptive immunereceptor comprises a plurality of J-segment oligonucleotide primers thatare provided in substantially equimolar amounts, and (iv) amplificationscales linearly with the number of starting templates of a givensequence.

Hence, an expected yield for the amplification product of each templatecan be calculated and arbitrarily assigned a theoretical uniformamplification level value of 100%. After permitting the primer sets toamplify the sequences of the template oligonucleotides in anamplification reaction, any statistically significant deviation fromsubstantial equivalence that is observed among the relative proportionsof distinct amplification products indicates that there has been bias(i.e., unequal efficiency) in primer utilization during amplification.In other words, quantitative differences in the relative amounts ofdifferent amplification products that are obtained indicate that not allprimers in the primer set have amplified their respective templates withcomparable efficiencies. Certain embodiments contemplate assigning arange of tolerances above and below a theoretical 100% yield, such thatany amplification level value within the range of tolerances may beregarded as substantial equivalence.

In certain such embodiments, the range of amplification product yieldsmay be regarded as substantially equivalent when the product yields areall within the same order of magnitude (e.g., differ by less than afactor of ten). In certain other such embodiments, the range ofamplification product yields may be regarded as substantially equivalentwhen the product yields differ from one another by no more thannine-fold, eight-fold, seven-fold, six-fold, five-fold, four-fold orthree-fold. In certain other embodiments, product yields that may beregarded as being within an acceptable tolerance range may be more orless than a calculated 100% yield by as much as 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60, 100, or200%.

Because the method involves determining the nucleotide sequence of eachamplification product using known techniques as part of thequantification process, the primer(s) responsible for amplification ofeach unique (as defined by sequence) product can be identified and theirrelative amount(s) in the primer set can be adjusted (e.g., increased ordecreased in a statistically significant manner) accordingly. Theconcentrations of excessively efficient primers in the primer set can bereduced relative to the concentrations of other primers, so that thelevel of specific amplification by such primers of templates in theherein described template composition is substantially equivalent to thelevel of amplification delivered by the majority of primers whichdeliver the theoretical uniform amplification level, or which deliver alevel that is within the acceptable tolerance range. The concentrationsof poorly efficient primers in the primer set can be increased relativeto the concentrations of other primers, so that the level of specificamplification by such primers of templates in the herein describedtemplate composition is substantially equivalent to the level ofamplification delivered by the majority of primers which deliver thetheoretical uniform amplification level, or which deliver a level withinthe acceptable tolerance range.

Accordingly and as described herein, there are thus presently provided atemplate composition for standardizing the amplification efficiency ofan oligonucleotide primer set that is designed to amplify codingsequences for a complete repertoire of a given TCR or Ig chain, a methodfor determining non-uniform amplification efficiency (“non-uniformamplification potential”) among members of such a primer set, and amethod for correcting such non-uniform amplification potential. Byproviding the herein described template composition as a standard withwhich oligonucleotide primer sets can be calibrated, and in particularembodiments, where each template oligonucleotide is present in asubstantially equimolar amount so that individual primer concentrationscan be adjusted to yield substantially uniform amplification of astructurally diverse array of amplification products, the presentdisclosure thus advantageously overcomes the above described problemsassociated with biases in individual primer efficiency.

Using the compositions and methods provided herein, individual primersmay be identified as having a non-uniform amplification potential byvirtue of their promotion of non-uniform amplification as evidenced byincreased (e.g., greater in a statistically significant manner) ordecreased (e.g., lower in a statistically significant manner)amplification of specific template oligonucleotides relative to theuniform amplification level, despite the presence in an amplificationreaction (i) of all template oligonucleotides in substantially equimolaramounts to one another, (ii) of all V-segment primers in substantiallyequimolar amounts to one another, and (iii) of all J-segment primers insubstantially equimolar amounts to one another.

The relative concentrations of such primers may then be decreased orincreased to obtain a modified complete set of primers in which allprimers are not present in substantially equimolar amounts relative toone another, to compensate, respectively, for the increased or decreasedlevel of amplification relative to the uniform amplification level. Theprimer set may then be retested for its ability to amplify all sequencesin the herein disclosed template composition at the uniformamplification level, or within an acceptable tolerance range.

The process of testing modified primer sets for their ability to amplifythe herein disclosed template composition, in which all templateoligonucleotides are provided in substantially equimolar amounts to oneanother, may be repeated iteratively until all products are amplified atthe uniform amplification level, or within an acceptable tolerancerange. By such a process using the herein disclosed templatecomposition, the amplification efficiency of an oligonucleotide primerset may be standardized, where the primer set is capable of amplifyingproductively rearranged DNA encoding one or a plurality of adaptiveimmune receptors in a biological sample that comprises DNA from lymphoidcells of a subject.

Additionally or alternatively, according to the present disclosure itmay be determined whether any particular pair of oligonucleotideamplification primers exhibits non-uniform amplification potential, suchas increased or decreased amplification of the template compositionrelative to a uniform amplification level exhibited by a majority of theoligonucleotide amplification primers, and a normalizing adjustmentfactor can then be used to calculate, respectively, a proportionatelydecreased or increased frequency of occurrence of the amplificationproducts that are promoted by each such amplification primer pair. Thepresent template compositions thus, in certain embodiments, provide amethod of correcting for non-uniform nucleic acid amplificationpotential among members of a set of oligonucleotide amplificationprimers.

Certain such embodiments may advantageously permit correction,calibration, standardization, normalization, or the like, of data thatare obtained as a consequence of non-uniform amplification events. Thus,the present embodiments permit correction of data inaccuracies, such asmay result from biased oligonucleotide primer utilization, without theneed for iteratively adjusting the concentrations of one or moreamplification primers and repeating the steps of amplifying the hereindescribed template compositions. Advantageous efficiencies may thus beobtained where repetition of the steps of quantitatively sequencing theamplification products can be avoided. Certain other contemplatedembodiments may, however, employ such an iterative approach.

Accordingly, and as described herein, there is presently provided atemplate composition for standardizing the amplification efficiency ofan oligonucleotide primer set, along with methods for using such atemplate composition to determine non-uniform nucleic acid amplificationpotential (e.g., bias) among individual members of the oligonucleotideprimer set. Also described herein are methods for correcting suchnon-uniform nucleic acid amplification potentials (e.g., biases) amongmembers of the oligonucleotide primer set. These and related embodimentsexploit previously unrecognized benefits that are obtained bycalibrating complex oligonucleotide primer sets to compensate forundesirable amplification biases using the template composition forstandardizing amplification efficiency having the features describedherein, and will find uses in improving the accuracy with which specificclonotypic TCR and/or Ig encoding DNA sequences can be quantified,relative to previously described methodologies.

As also noted above and described elsewhere herein, prior to the presentdisclosure there existed unsatisfactory and difficult-to-discerndiscrepancies between (i) the actual quantitative distribution ofrearranged adaptive immune receptor-encoding DNA templates having uniquesequences in a biological sample comprising lymphoid cell DNA from asubject, and (ii) the relative representation of nucleic acidamplification products of such templates, following multiplexedamplification using a complex set of oligonucleotide amplificationprimers designed to amplify substantially all productively rearrangedadaptive immune receptor genes in the sample. Due to, e.g., theheterogeneity of both the template population and the amplificationprimer set, and as shown herein, significant disparities in theamplification efficiencies of different amplification primers may becommon, leading to substantial skewing in the relative proportions ofamplification products that are obtained and quantitatively sequencedfollowing an amplification reaction.

Templates and Primers

According to certain preferred embodiments there is thus provided atemplate composition for standardizing the amplification efficiency ofan oligonucleotide primer set that is capable of amplifying rearrangedDNA (which in certain embodiments may refer to productively rearrangedDNA but which in certain other embodiments need not be so limited)encoding one or a plurality of adaptive immune receptors in a biologicalsample that comprises DNA from lymphoid cells of a subject, the templatecomposition comprising a plurality of template oligonucleotides ofgeneral formula (I):5′-U1-B1-V-B2-R-B3-J-B4-U2-3′  (I)

as provided herein. In certain preferred embodiments each templateoligonucleotide in the plurality of template oligonucleotides is presentin a substantially equimolar amount, which in certain embodiments and asnoted above may refer to a composition in which each of the templateoligonucleotides is present at an equimolar concentration or at a molarconcentration that deviates from equimolar by no more than 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 50, 60, 70, 80, 90, 100 or 200% on a molar basis, and which incertain other embodiments may refer to a composition in which all of thetemplate oligonucleotides are present at molar concentrations that arewithin an order of magnitude of one another. The plurality of templatesmay comprise at least 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000,1100 or more discrete oligonucleotide species each having a distinctnucleotide sequence, including every intermediate integer valuetherebetween.

The herein disclosed template composition thus comprises a plurality oftemplate oligonucleotides of general formula:5′-U1-B1-V-B2-R-B3-J-B4-U2-3′  [I]

wherein, briefly and as elaborated in greater detail elsewhere herein,according to certain preferred embodiments:

V is a polynucleotide comprising at least 20, 30, 60, 90, 120, 150, 180,or 210, and not more than 1000, 900, 800, 700, 600 or 500 contiguousnucleotides of an adaptive immune receptor variable (V) region encodinggene sequence, or the complement thereof, and in each of the pluralityof template oligonucleotide sequences V comprises a uniqueoligonucleotide sequence;

J is a polynucleotide comprising at least 15-30, 31-60, 61-90, 91-120,or 120-150, and not more than 600, 500, 400, 300 or 200 contiguousnucleotides of an adaptive immune receptor joining (J) region encodinggene sequence, or the complement thereof, and in each of the pluralityof template oligonucleotide sequences J comprises a uniqueoligonucleotide sequence;

U1 and U2 are each either nothing or each comprise an oligonucleotidehaving, independently, a sequence that is selected from (i) a universaladaptor oligonucleotide sequence, and (ii) a sequencingplatform-specific oligonucleotide sequence that is linked to andpositioned 5′ to the universal adaptor oligonucleotide sequence;

B1, B2, B3, and B4 are each independently either nothing or eachcomprise an oligonucleotide B that comprises an oligonucleotide barcodesequence of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500,600, 700, 800, 900 or 1000 contiguous nucleotides (including all integervalues therebetween), wherein in each of the plurality of templateoligonucleotide sequences B comprises a unique oligonucleotide sequencethat uniquely identifies, or identifies as a paired combination, (i) theunique V oligonucleotide sequence of the template oligonucleotide and(ii) the unique J oligonucleotide sequence of the templateoligonucleotide; and

R is either nothing or comprises a restriction enzyme recognition sitethat comprises an oligonucleotide sequence that is absent from V, J, U1,U2, B1, B2, B3, and B4.

In some embodiments, the template oligonucleotide composition comprisesadditional non-coding or random oligonucleotides. These oligonucleotidesmay be inserted in various sections between or within the components inthe general formula T (5′-U1-B1-V-B2-R-B3-J-B4-U2-3′) and be of variouslengths in size.

In one embodiment, a is 1 to a number of maximum V gene segments in themammalian genome of the subject. In another embodiment, b is 1 to anumber of maximum J gene segments in the mammalian genome of thesubject. In other embodiments, a is 1 or b is 1. In some embodiments, acan range from 1 V gene segment to 54 V gene segments for TCRA, 1-76 Vgene segments for TCRB, 1-15 V gene segments for TCRG, 1-7 V genesegments for TCRD, 1-165 V gene segments for IGH, 1-111 for IGK, or 1-79V gene segments for IGL. In other embodiments, b can range from 1 J genesegment to 61 J gene segments for TCRA, 1-14 J gene segments for TCRB,1-5 J gene segments for TCRG, 1-4 gene segments for TCRD, 1-9 J genesegments for IGH, 1-5 J gene segments for IGK, or 1-11 J gene segmentsfor IGL.

The table below lists the number of V gene segments (a) and J genesegments (b) for each human adaptive immune receptor loci, includingfunctional V and J segments.

functional V Functional J V segments* segments** J segments* segments**TCRA 54 45 61 50 TCRB 76 48 14 13 TCRG 15 6 5 5 TCRD 7 7 4 4 IGH 165 519 6 IGK 111 44 5 5 IGL 79 33 11 7 *Total variable and joining segmentgenes **Variable and joining segment genes with at least one functionalallele

In some embodiments, the J polynucleotide comprises at least 15-30,31-60, 61-90, 91-120, or 120-150, and not more than 600, 500, 400, 300or 200 contiguous nucleotides of an adaptive immune receptor J constantregion, or the complement thereof.

In certain embodiments the plurality of template oligonucleotidescomprises at least (a×b) unique oligonucleotide sequences, where a isthe number of unique adaptive immune receptor V region-encoding genesegments in a subject and b is the number of unique adaptive immunereceptor J region-encoding gene segments in the subject, and thecomposition comprises at least one template oligonucleotide for everypossible combination of a V region-encoding gene segment and a Jregion-encoding gene segment.

The presently contemplated invention is not intended to be so limited,however, such that in certain embodiments, a substantially fewer numberof template oligonucleotides may advantageously be used. In these andrelated embodiments, where a is the number of unique adaptive immunereceptor V region-encoding gene segments in a subject and b is thenumber of unique adaptive immune receptor J region-encoding genesegments in the subject, the minimum number of unique oligonucleotidesequences of which the plurality of template oligonucleotides iscomprised may be determined by whichever is the larger of a and b, solong as each unique V polynucleotide sequence and each unique Jpolynucleotide sequence is present in at least one templateoligonucleotide in the template composition. Thus, according to certainrelated embodiments the template composition may comprise at least onetemplate oligonucleotide for each unique V polynucleotide, e.g., thatincludes a single one of each unique V polynucleotide according togeneral formula (I), and at least one template oligonucleotide for eachunique J polynucleotide, e.g., that includes a single one of each uniqueJ polynucleotide according to general formula (I).

In certain other embodiments, the template composition comprises atleast one template oligonucleotide to which each oligonucleotideamplification primer in an amplification primer set can anneal.

That is, in certain embodiments, the template composition comprises atleast one template oligonucleotide having an oligonucleotide sequence ofgeneral formula (I) to which each V-segment oligonucleotide primer canspecifically hybridize, and at least one template oligonucleotide havingan oligonucleotide sequence of general formula (I) to which eachJ-segment oligonucleotide primer can specifically hybridize.

According to such embodiments the oligonucleotide primer set that iscapable of amplifying rearranged DNA encoding one or a plurality ofadaptive immune receptors comprises a plurality a′ of unique V-segmentoligonucleotide primers and a plurality b′ of unique J-segmentoligonucleotide primers. The plurality of a′ V-segment oligonucleotideprimers are each independently capable of annealing or specificallyhybridizing to at least one polynucleotide encoding an adaptive immunereceptor V-region polypeptide or to the complement thereof, wherein eachV-segment primer comprises a nucleotide sequence of at least 15contiguous nucleotides that is complementary to at least one adaptiveimmune receptor V region-encoding gene segment. The plurality of b′0.1-segment oligonucleotide primers are each independently capable ofannealing or specifically hybridizing to at least one polynucleotideencoding an adaptive immune receptor J-region polypeptide or to thecomplement thereof, wherein each J-segment primer comprises a nucleotidesequence of at least 15 contiguous nucleotides that is complementary toat least one adaptive immune receptor J region-encoding gene segment.

In some embodiments, a′ is the same as a (described above for templateoligonucleotides). In other embodiments, b′ is the same as b (describedabove for template oligonucleotides).

Thus, in certain embodiments and as also discussed elsewhere herein, thepresent template composition may be used in amplification reactions withamplification primers that are designed to amplify all rearrangedadaptive immune receptor encoding gene sequences, including those thatare not expressed, while in certain other embodiments the templatecomposition and amplification primers may be designed so as not to yieldamplification products of rearranged genes that are not expressed (e.g.,pseudogenes, orphons). It will therefore be appreciated that in certainembodiments only a subset of rearranged adaptive immune receptorencoding genes may desirably be amplified, such that suitableamplification primer subsets may be designed and employed to amplifyonly those rearranged V−J sequences that are of interest. In these andrelated embodiments, correspondingly, a herein described templatecomposition comprising only a subset of interest of rearranged V−Jrearranged sequences may be used, so long as the template compositioncomprises at least one template oligonucleotide to which eacholigonucleotide amplification primer in an amplification primer set cananneal. The actual number of template oligonucleotides in the templatecomposition may thus vary considerably among the contemplatedembodiments, as a function of the amplification primer set that is to beused.

For example, in certain related embodiments, in the template compositionthe plurality of template oligonucleotides may have a plurality ofsequences of general formula (I) that is selected from (1) the pluralityof oligonucleotide sequences of general formula (I) in whichpolynucleotides V and J have the TCRB V and J sequences set forth in atleast one set of 68 TCRB V and J SEQ ID NOS, respectively, as set forthin FIGS. 5a-5l as TCRB V/J set 1, TCRB V/J set 2, TCRB V/J set 3, TCRBV/J set 4, TCRB V/J set 5, TCRB V/J set 6, TCRB V/J set 7, TCRB V/J set8, TCRB V/J set 9, TCRB V/J set 10, TCRB V/J set 11, TCRB V/J set 12 andTCRB V/J set 13; (2) the plurality of oligonucleotide sequences ofgeneral formula (I) in which polynucleotides V and J have the TCRG V andJ sequences set forth in at least one set of 14 TCRG V and J SEQ ID NOS,respectively, as set forth in FIG. 6 as TCRG V/J set 1, TCRG V/J set 2,TCRG V/J set 3, TCRG V/J set 4 and TCRG V/J set 5; and (3) the pluralityof oligonucleotide sequences of general formula (I) in whichpolynucleotides V and J have the IGH V and J sequences set forth in atleast one set of 127 IGH V and J SEQ ID NOS, respectively, as set forthin FIG. 7 as IGH V/J set 1, IGH V/J set 2, IGH V/J set 3, IGH V/J set 4,IGH V/J set 5, IGH V/J set 6, IGH V/J set 7, IGH V/J set 8 and IGH V/Jset 9.

In certain embodiments, V is a polynucleotide sequence that encodes atleast 10-70 contiguous amino acids of an adaptive immune receptorV-region, or the complement thereof; J is a polynucleotide sequence thatencodes at least 5-30 contiguous amino acids of an adaptive immunereceptor J-region, or the complement thereof; U1 and U2 are each eithernothing or comprise an oligonucleotide comprising a nucleotide sequencethat is selected from (i) a universal adaptor oligonucleotide sequence,and (ii) a sequencing platform-specific oligonucleotide sequence that islinked to and positioned 5′ to the universal adaptor oligonucleotidesequence; B1, B2, B3 and B4 are each independently either nothing oreach comprise an oligonucleotide B that comprises an oligonucleotidebarcode sequence of 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19 or 20 contiguous nucleotides, wherein in each of the plurality ofoligonucleotide sequences B comprises a unique oligonucleotide sequencethat uniquely identifies, as a paired combination, (i) the unique Voligonucleotide sequence and (ii) the unique J oligonucleotide sequence;and R is either nothing or comprises a restriction enzyme recognitionsite that comprises an oligonucleotide sequence that is absent from V,J, U1, U2, B1, B2, B3, and B4. In certain preferred embodiments theplurality of template oligonucleotides comprises at least either a or bunique oligonucleotide sequences, where a is the number of uniqueadaptive immune receptor V region-encoding gene segments in the subjectand b is the number of unique adaptive immune receptor J region-encodinggene segments in the subject, and the composition comprises a pluralityof template oligonucleotides that comprise at least whichever is thegreater of a or b unique template oligonucleotide sequences, providedthat at least one V polynucleotide corresponding to each Vregion-encoding gene segment and at least one J polynucleotidecorresponding to each J region-encoding gene segment is included.

A large number of adaptive immune receptor variable (V) region andjoining (J) region gene sequences are known as nucleotide and/or aminoacid sequences, including non-rearranged genomic DNA sequences of TCRand Ig loci, and productively rearranged DNA sequences at such loci andtheir encoded products, and also including pseudogenes at these loci,and also including related orphons. See, e.g., U.S. application Ser. No.13/217,126; U.S. application Ser. No. 12/794,507; PCT/US2011/026373;PCT/US2011/049012. These and other sequences known to the art may beused according to the present disclosure for the design and productionof template oligonucleotides to be included in the presently providedtemplate composition for standardizing amplification efficiency of anoligonucleotide primer set, and for the design and production of theoligonucleotide primer set that is capable of amplifying rearranged DNAencoding TCR or Ig polypeptide chains, which rearranged DNA may bepresent in a biological sample comprising lymphoid cell DNA.

In formula (I), V is a polynucleotide sequence of at least 30, 40, 50,60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200,210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340,350, 360, 370, 380, 390, 400 or 450 and not more than 1000, 900, 800,700, 600 or 500 contiguous nucleotides of an adaptive immune receptor(e.g., TCR or BCR) variable (V) region gene sequence, or the complementthereof, and in each of the plurality of oligonucleotide sequences Vcomprises a unique oligonucleotide sequence. Genomic sequences for TCRand BCR V region genes of humans and other species are known andavailable from public databases such as Genbank; V region gene sequencesinclude polynucleotide sequences that encode the products of expressed,rearranged TCR and BCR genes and also include polynucleotide sequencesof pseudogenes that have been identified in the V region loci. Thediverse V polynucleotide sequences that may be incorporated into thepresently disclosed templates of general formula (I) may vary widely inlength, in nucleotide composition (e.g., GC content), and in actuallinear polynucleotide sequence, and are known, for example, to include“hot spots” or hypervariable regions that exhibit particular sequencediversity.

The polynucleotide V in general formula (I) (or its complement) includessequences to which members of oligonucleotide primer sets specific forTCR or BCR genes can specifically anneal. Primer sets that are capableof amplifying rearranged DNA encoding a plurality of TCR or BCR aredescribed, for example, in U.S. application Ser. No. 13/217,126; U.S.application Ser. No. 12/794,507; PCT/US2011/026373; orPCT/US2011/049012; or the like; or as described therein may be designedto include oligonucleotide sequences that can specifically hybridize toeach unique V gene and to each J gene in a particular TCR or BCR genelocus (e.g., TCR α, β, γ or γ, or IgH μ, γ, δ, α or ε, or IgL κ or λ).For example by way of illustration and not limitation, anoligonucleotide primer of an oligonucleotide primer amplification setthat is capable of amplifying rearranged DNA encoding one or a pluralityof TCR or BCR may typically include a nucleotide sequence of 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39 or 40 contiguous nucleotides, or more, and mayspecifically anneal to a complementary sequence of 15, 16, 17, 18, 19,20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37,38, 39 or 40 contiguous nucleotides of a V or a J polynucleotide asprovided herein. In certain embodiments the primers may comprise atleast 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 nucleotides, andin certain embodiment the primers may comprise sequences of no more than15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39 or 40 contiguous nucleotides. Primers andprimer annealing sites of other lengths are also expressly contemplated,as disclosed herein.

The entire polynucleotide sequence of each polynucleotide V in generalformula (I) may, but need not, consist exclusively of contiguousnucleotides from each distinct V gene. For example and according tocertain embodiments, in the template composition described herein, eachpolynucleotide V of formula (I) need only have at least a regioncomprising a unique V oligonucleotide sequence that is found in one Vgene and to which a single V region primer in the primer set canspecifically anneal. Thus, the V polynucleotide of formula (I) maycomprise all or any prescribed portion (e.g., at least 15, 20, 30, 60,90, 120, 150, 180 or 210 contiguous nucleotides, or any integer valuetherebetween) of a naturally occurring V gene sequence (including a Vpseudogene sequence) so long as at least one unique V oligonucleotidesequence region (the primer annealing site) is included that is notincluded in any other template V polynucleotide.

It may be preferred in certain embodiments that the plurality of Vpolynucleotides that are present in the herein described templatecomposition have lengths that simulate the overall lengths of known,naturally occurring V gene nucleotide sequences, even where the specificnucleotide sequences differ between the template V region and anynaturally occurring V gene. The V region lengths in the herein describedtemplates may differ from the lengths of naturally occurring V genesequences by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19 or 20 percent.

The V polynucleotide in formula (I) may thus, in certain embodiments,comprise a nucleotide sequence having a length that is the same orsimilar to that of the length of a typical V gene from its start codonto its CDR3 encoding region and may, but need not, include a nucleotidesequence that encodes the CDR3 region. CDR3 encoding nucleotidesequences and sequence lengths may vary considerably and have beencharacterized by several different numbering schemes (e.g., Lefranc,1999 The Immunologist 7:132; Kabat et al., 1991 In: Sequences ofProteins of Immunological Interest, NIH Publication 91-3242; Chothia etal., 1987 J. Mol. Biol. 196:901; Chothia et al., 1989 Nature 342:877;Al-Lazikani et al., 1997 J. Mol. Biol. 273:927; see also, e.g., Rock etal., 1994 J. Exp. Med. 179:323; Saada et al., 2007 Immunol. Cell Biol.85:323).

Briefly, the CDR3 region typically spans the polypeptide portionextending from a highly conserved cysteine residue (encoded by thetrinucleotide codon TGY; Y=T or C) in the V segment to a highlyconserved phenylalanine residue (encoded by TTY) in the J segment ofTCRs, or to a highly conserved tryptophan (encoded by TGG) in IGH. Morethan 90% of natural, productive rearrangements in the TCRB locus have aCDR3 encoding length by this criterion of between 24 and 54 nucleotides,corresponding to between 9 and 17 encoded amino acids. The CDR3 lengthsof the presently disclosed synthetic template oligonucleotides should,for any given TCR or BCR locus, fall within the same range as 95% ofnaturally occurring rearrangements. Thus, for example, in a hereindescribed template composition for standardizing the amplificationefficiency of an oligonucleotide primer set that is capable ofamplifying rearranged DNA encoding a plurality of TCRB polypeptides, theCDR3 encoding portion of the V polynucleotide may have a length of from24 to 54 nucleotides, including every integer therebetween. Thenumbering schemes for CDR3 encoding regions described above denote thepositions of the conserved cysteine, phenylalanine and tryptophancodons, and these numbering schemes may also be applied to pseudogenesin which one or more codons encoding these conserved amino acids mayhave been replaced with a codon encoding a different amino acid. Forpseudogenes which do not use these conserved amino acids, the CDR3length may be defined relative to the corresponding position at whichthe conserved residue would have been observed absent the substitution,according to one of the established CDR3 sequence position numberingschemes referenced above.

It may also be preferred, in certain embodiments, that the plurality ofV polynucleotides that are present in the herein described templatecomposition have nucleotide compositions (e.g., percentage of GCcontent) that simulate the overall nucleotide compositions of known,naturally occurring V gene sequences, even where the specific nucleotidesequences differ. Such template V region nucleotide compositions maydiffer from the nucleotide compositions of naturally occurring V genesequences by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19 or 20 percent. Optionally and according to certainembodiments, the V polynucleotide of the herein described templateoligonucleotide includes a stop codon at or near the 3′ end of V ingeneral formula (I).

In formula (I) J is a polynucleotide comprising at least 15-30, 31-60,61-90, 91-120, or 120-150, and not more than 600, 500, 400, 300 or 200contiguous nucleotides of an adaptive immune receptor joining (J) regionencoding gene sequence, or the complement thereof, and in each of theplurality of oligonucleotide sequences J comprises a uniqueoligonucleotide sequence.

The polynucleotide J in general formula (I) (or its complement) includessequences to which members of oligonucleotide primer sets specific forTCR or BCR genes can specifically anneal. Primer sets that are capableof amplifying rearranged DNA encoding a plurality of TCR or BCR aredescribed, for example, in U.S. application Ser. No. 13/217,126; U.S.application Ser. No. 12/794,507; PCT/US2011/026373; orPCT/US2011/049012; or the like; or as described therein may be designedto include oligonucleotide sequences that can specifically hybridize toeach unique V gene and to each unique J gene in a particular TCR or BCRgene locus (e.g., TCR α, β, γ or δ, or IgH μ, γ, δ, α or ε, or IgL κ orλ).

The entire polynucleotide sequence of each polynucleotide J in generalformula (I) may, but need not, consist exclusively of contiguousnucleotides from each distinct J gene. For example and according tocertain embodiments, in the template composition described herein, eachpolynucleotide J of formula (I) need only have at least a regioncomprising a unique J oligonucleotide sequence that is found in one Jgene and to which a single V region primer in the primer set canspecifically anneal. Thus, the V polynucleotide of formula (I) maycomprise all or any prescribed portion (e.g., at least 15, 20, 30, 60,90, 120, 150, 180 or 210 contiguous nucleotides, or any integer valuetherebetween) of a naturally occurring V gene sequence (including a Vpseudogene sequence) so long as at least one unique V oligonucleotidesequence region (the primer annealing site) is included that is notincluded in any other template J polynucleotide.

It may be preferred in certain embodiments that the plurality of Jpolynucleotides that are present in the herein described templatecomposition have lengths that simulate the overall lengths of known,naturally occurring J gene nucleotide sequences, even where the specificnucleotide sequences differ between the template J region and anynaturally occurring J gene. The J region lengths in the herein describedtemplates may differ from the lengths of naturally occurring J genesequences by no more than 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19 or 20 percent.

The J polynucleotide in formula (I) may thus, in certain embodiments,comprise a nucleotide sequence having a length that is the same orsimilar to that of the length of a typical naturally occurring J geneand may, but need not, include a nucleotide sequence that encodes theCDR3 region, as discussed above.

Genomic sequences for TCR and BCR J region genes of humans and otherspecies are known and available from public databases such as Genbank; Jregion gene sequences include polynucleotide sequences that encode theproducts of expressed and unexpressed rearranged TCR and BCR genes. Thediverse J polynucleotide sequences that may be incorporated into thepresently disclosed templates of general formula (I) may vary widely inlength, in nucleotide composition (e.g., GC content), and in actuallinear polynucleotide sequence.

Alternatives to the V and J sequences described herein, for use inconstruction of the herein described template oligonucleotides and/orV-segment and J-segment oligonucleotide primers, may be selected by askilled person based on the present disclosure using knowledge in theart regarding published gene sequences for the V- and J-encoding regionsof the genes for each TCR and Ig subunit. Reference Genbank entries forhuman adaptive immune receptor sequences include: TCRα: (TCRA/D):NC_000014.8 (chr14:22090057 . . . 23021075); TCRβ: (TCRB): NC_000007.13(chr7:141998851 . . . 142510972); TCRγ: (TCRG): NC_000007.13(chr7:38279625 . . . 38407656); immunoglobulin heavy chain, IgH (IGH):NC_000014.8 (chr14: 106032614 . . . 107288051); immunoglobulin lightchain-kappa, IgLκ (IGK): NC_000002.11 (chr2: 89156874 . . . 90274235);and immunoglobulin light chain-lambda, IgLλ (IGL): NC_000022.10 (chr22:22380474 . . . 23265085). Reference Genbank entries for mouse adaptiveimmune receptor loci sequences include: TCRβ: (TCRB): NC_000072.5 (chr6:40841295 . . . 41508370), and immunoglobulin heavy chain, IgH (IGH):NC_000078.5 (chr12:114496979 . . . 117248165).

Template and primer design analyses and target site selectionconsiderations can be performed, for example, using the OLIGO primeranalysis software and/or the BLASTN 2.0.5 algorithm software (Altschulet al., Nucleic Acids Res. 1997, 25(17):3389-402), or other similarprograms available in the art.

Accordingly, based on the present disclosure and in view of these knownadaptive immune receptor gene sequences and oligonucleotide designmethodologies, for inclusion in the instant template oligonucleotidesthose skilled in the art can design a plurality of V region-specific andJ region-specific polynucleotide sequences that each independentlycontain oligonucleotide sequences that are unique to a given V and Jgene, respectively. Similarly, from the present disclosure and in viewof known adaptive immune receptor sequences, those skilled in the artcan also design a primer set comprising a plurality of V region-specificand J region-specific oligonucleotide primers that are eachindependently capable of annealing to a specific sequence that is uniqueto a given V and J gene, respectively, whereby the plurality of primersis capable of amplifying substantially all V genes and substantially allJ genes in a given adaptive immune receptor-encoding locus (e.g., ahuman TCR or IgH locus). Such primer sets permit generation, inmultiplexed (e.g., using multiple forward and reverse primer pairs) PCR,of amplification products that have a first end that is encoded by arearranged V region-encoding gene segment and a second end that isencoded by a J region-encoding gene segment.

Typically and in certain embodiments, such amplification products mayinclude a CDR3-encoding sequence although the invention is not intendedto be so limited and contemplates amplification products that do notinclude a CDR3-encoding sequence. The primers may be preferably designedto yield amplification products having sufficient portions of V and Jsequences and/or of V−J barcode (B) sequences as described herein, suchthat by sequencing the products (amplicons), it is possible to identifyon the basis of sequences that are unique to each gene segment (i) theparticular V gene, and (ii) the particular J gene in the proximity ofwhich the V gene underwent rearrangement to yield a functional adaptiveimmune receptor-encoding gene. Typically, and in preferred embodiments,the PCR amplification products will not be more than 600 base pairs insize, which according to non-limiting theory will exclude amplificationproducts from non-rearranged adaptive immune receptor genes. In certainother preferred embodiments the amplification products will not be morethan 500, 400, 300, 250, 200, 150, 125, 100, 90, 80, 70, 60, 50, 40, 30or 20 base pairs in size, such as may advantageously provide rapid,high-throughput quantification of sequence-distinct amplicons by shortsequence reads.

In certain preferred embodiments, the plurality of templateoligonucleotides comprises at least a or at least b uniqueoligonucleotide sequences, whichever is larger, where a is the number ofunique adaptive immune receptor V region-encoding gene segments in thesubject and b is the number of unique adaptive immune receptor Jregion-encoding gene segments in the subject, and the compositioncomprises at least one template oligonucleotide for each unique Vpolynucleotide and at least one template oligonucleotide for each uniqueJ polynucleotide. It will be appreciated that because the templateoligonucleotides have a plurality of oligonucleotide sequences ofgeneral formula (I), which includes a V polynucleotide and which alsoincludes a J polynucleotide, that the template composition may thuscomprise fewer than (a×b) unique oligonucleotide sequences, but willcomprise at least the larger of a or b unique oligonucleotide sequences.Accordingly, the composition may accommodate at least one occurrence ofeach unique V polynucleotide sequence and at least one occurrence ofeach unique J polynucleotide sequence, where in some instances the atleast one occurrence of a particular unique V polynucleotide will bepresent in the same template oligonucleotide in which may be found theat least one occurrence of a particular unique J polynucleotide. Thus,for example, “at least one template oligonucleotide for each unique Vpolynucleotide and at least one template oligonucleotide for each uniqueJ polynucleotide” may in certain instances refer to a single templateoligonucleotide in which one unique V polynucleotide and one unique Jpolynucleotide are present.

As also disclosed elsewhere herein, in certain other preferredembodiments the template composition comprises at least one templateoligonucleotide to which each oligonucleotide amplification primer in anamplification primer set can anneal. Hence, the composition may comprisefewer than a or b unique sequences, for example, where an amplificationprimer set may not include a unique primer for every possible V and/or Jsequence.

It will be noted that certain embodiments contemplate a templatecomposition for standardizing the amplification efficiency of anoligonucleotide primer set that is capable of amplifying productivelyrearranged DNA encoding one or a plurality of adaptive immune receptorsin a biological sample that comprises DNA from lymphoid cells of asubject as provided herein, wherein the template composition comprises aplurality of template oligonucleotides having a plurality ofoligonucleotide sequences of general formula5′-U1-B1-V-B2-R-B3-J-B4-U2-3′ (I) as described herein. According tothese and related embodiments and as also described elsewhere herein,the set of oligonucleotide amplification primers that is capable ofamplifying productively rearranged DNA may exclude any oligonucleotideprimers that specifically hybridize to a V-region pseudogene or orphonor to a J-region pseudogene or orphon. Hence, in such embodiments thetemplate composition will desirably exclude template oligonucleotides ofgeneral formula (I) in which unique V oligonucleotide sequences and/orunique J oligonucleotide sequences are sequences that are, respectively,unique to a V-region pseudogene or orphon or to a J-region pseudogene ororphon.

An exemplary TCRB template composition comprising 858 distinct templateoligonucleotides is disclosed in the Sequence Listing in SEQ IDNOS:3157-4014. Another exemplary TCRB template composition comprising871 distinct template oligonucleotides is disclosed in the SequenceListing in SEQ ID NOS:1-871. Another exemplary TCRB template compositioncomprising 689 distinct template oligonucleotides is disclosed in theSequence Listing in SEQ ID NOS:872-1560.

An exemplary TCRG template composition comprising 70 distinct templateoligonucleotides is disclosed in the Sequence Listing in SEQ IDNOS:4015-4084. An exemplary TCRG template composition comprising 70distinct template oligonucleotides is also disclosed in the SequenceListing in SEQ ID NOS:1561-1630.

An exemplary IGH template composition comprising 1116 distinct templateoligonucleotides is disclosed in the Sequence Listing in SEQ IDNOS:4085-5200. An exemplary IGH template composition comprising 1116distinct template oligonucleotides is also disclosed in the SequenceListing in SEQ ID NOS:1805-2920.

Also disclosed herein are exemplary sets of V and J polynucleotides forinclusion in the herein described template oligonucleotides having aplurality of oligonucleotide sequences of general formula (I). For TCRB,the plurality of template oligonucleotides may have a plurality ofoligonucleotide sequences of general formula (I) in whichpolynucleotides V and J have the TCRB V and J sequences set forth in atleast one set of 68 TCRB V and J SEQ ID NOS, respectively, as set forthin FIG. 5 as TCRB V/J set 1, TCRB V/J set 2, TCRB V/J set 3, TCRB V/Jset 4, TCRB V/J set 5, TCRB V/J set 6, TCRB V/J set 7, TCRB V/J set 8,TCRB V/J set 9, TCRB V/J set 10, TCRB V/J set 11, TCRB V/J set 12 andTCRB V/J set 13.

For TCRG, the plurality of template oligonucleotides may have aplurality of oligonucleotide sequences of general formula (I) in whichpolynucleotides V and J have the TCRG V and J sequences set forth in atleast one set of 14 TCRG V and J SEQ ID NOS, respectively, as set forthin FIG. 6 as TCRG V/J set 1, TCRG V/J set 2, TCRG V/J set 3, TCRG V/Jset 4 and TCRG V/J set 5.

For IGH, the plurality of template oligonucleotides may have a pluralityof oligonucleotide sequences of general formula (I) in whichpolynucleotides V and J have the IGH V and J sequences set forth in atleast one set of 127 IGH V and J SEQ ID NOS, respectively, as set forthin FIG. 7 as IGH V/J set 1, IGH V/J set 2, IGH V/J set 3, IGH V/J set 4,IGH V/J set 5, IGH V/J set 6, IGH V/J set 7, IGH V/J set 8 and IGH V/Jset 9.

Primers

According to the present disclosure, oligonucleotide primers areprovided in an oligonucleotide primer set that comprises a plurality ofV-segment primers and a plurality of J-segment primers, where the primerset is capable of amplifying rearranged DNA encoding adaptive immunereceptors in a biological sample that comprises lymphoid cell DNA.Suitable primer sets are known in the art and disclosed herein, forexample, the primer sets in U.S. application Ser. No. 13/217,126; U.S.application Ser. No. 12/794,507; PCT/US2011/026373; orPCT/US2011/049012; or the like; or those shown in Table 1. In certainembodiments the primer set is designed to include a plurality of Vsequence-specific primers that includes, for each unique V region gene(including pseudogenes) in a sample, at least one primer that canspecifically anneal to a unique V region sequence; and for each unique Jregion gene in the sample, at least one primer that can specificallyanneal to a unique J region sequence.

Primer design may be achieved by routine methodologies in view of knownTCR and BCR genomic sequences. Accordingly, the primer set is preferablycapable of amplifying every possible V−J combination that may resultfrom DNA rearrangements in the TCR or BCR locus. As also describedbelow, certain embodiments contemplate primer sets in which one or moreV primers may be capable of specifically annealing to a “unique”sequence that may be shared by two or more V regions but that is notcommon to all V regions, and/or in which in which one or more J primersmay be capable of specifically annealing to a “unique” sequence that maybe shared by two or more J regions but that is not common to all Jregions.

In particular embodiments, oligonucleotide primers for use in thecompositions and methods described herein may comprise or consist of anucleic acid of at least about 15 nucleotides long that has the samesequence as, or is complementary to, a 15 nucleotide long contiguoussequence of the target V- or J-segment (i.e., portion of genomicpolynucleotide encoding a V-region or J-region polypeptide). Longerprimers, e.g., those of about 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, or 50,nucleotides long that have the same sequence as, or sequencecomplementary to, a contiguous sequence of the target V- or J-regionencoding polynucleotide segment, will also be of use in certainembodiments. All intermediate lengths of the presently describedoligonucleotide primers are contemplated for use herein. As would berecognized by the skilled person, the primers may have additionalsequence added (e.g., nucleotides that may not be the same as orcomplementary to the target V- or J-region encoding polynucleotidesegment), such as restriction enzyme recognition sites, adaptorsequences for sequencing, bar code sequences, and the like (see e.g.,primer sequences provided in the Tables and sequence listing herein).Therefore, the length of the primers may be longer, such as about 55,56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,74, 75, 80, 85, 90, 95, 100 or more nucleotides in length or more,depending on the specific use or need.

Also contemplated for use in certain embodiments are adaptive immunereceptor V-segment or J-segment oligonucleotide primer variants that mayshare a high degree of sequence identity to the oligonucleotide primersfor which nucleotide sequences are presented herein, including those setforth in the Sequence Listing. Thus, in these and related embodiments,adaptive immune receptor V-segment or J-segment oligonucleotide primervariants may have substantial identity to the adaptive immune receptorV-segment or J-segment oligonucleotide primer sequences disclosedherein, for example, such oligonucleotide primer variants may compriseat least 70% sequence identity, preferably at least 75%, 80%, 85%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% or higher sequenceidentity compared to a reference polynucleotide sequence such as theoligonucleotide primer sequences disclosed herein, using the methodsdescribed herein (e.g., BLAST analysis using standard parameters). Oneskilled in this art will recognize that these values can beappropriately adjusted to determine corresponding ability of anoligonucleotide primer variant to anneal to an adaptive immune receptorsegment-encoding polynucleotide by taking into account codon degeneracy,reading frame positioning and the like.

Typically, oligonucleotide primer variants will contain one or moresubstitutions, additions, deletions and/or insertions, preferably suchthat the annealing ability of the variant oligonucleotide is notsubstantially diminished relative to that of an adaptive immune receptorV-segment or J-segment oligonucleotide primer sequence that isspecifically set forth herein.

Table 1 presents as a non-limiting example an oligonucleotide primer setthat is capable of amplifying productively rearranged DNA encoding TCRβ-chains (TCRB) in a biological sample that comprises DNA from lymphoidcells of a subject. In this primer set the J segment primers sharesubstantial sequence homology, and therefore may cross-prime amongstmore than one target J polynucleotide sequence, but the V segmentprimers are designed to anneal specifically to target sequences withinthe CDR2 region of V and are therefore unique to each V segment. Anexception, however, is present in the case of several V primers wherethe within-family sequences of the closely related target genes areidentical (e.g., V6-2 and V6-3 are identical at the nucleotide levelthroughout the coding sequence of the V segment, and therefore may havea single primer, TRB2V6-2/3).

It will therefore be appreciated that in certain embodiments the numberof different template oligonucleotides in the template composition,and/or the number of different oligonucleotide primers in the primerset, may be advantageously reduced by designing template and/or primersto exploit certain known similarities in V and/or J sequences. Thus, inthese and related embodiments, “unique” oligonucleotide sequences asdescribed herein may include specific V polynucleotide sequences thatare shared by 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19 or 20 distinct template oligonucleotides and/or specific Jpolynucleotide sequences that are shared by 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12 or 13 distinct template oligonucleotides, where suchtemplates differ in sequence from one another by other than the shared Vand/or J sequences.

According to certain presently contemplated embodiments, it may beuseful to decrease (e.g., reduce in a statistically significant manner)template amplification bias such as non-uniform nucleic acidamplification potential among members of a set of amplification primersthat can result from unequal primer efficiencies (e.g., unequal primerutilization) only for a limited subset of all naturally occurring V andJ genes. For example, in analyses of the TCR or BCR immune repertoireinvolved in an immune response, whether to a specific antigen, as in avaccine, or to a tissue, as in an autoimmune disease, only theproductive TCR or IG rearrangements may be of interest. In suchcircumstances, it may be economically advantageous to identify andcorrect non-uniform nucleic acid amplification potential only for thoseV and J segment primers that contribute to productive rearrangements ofTCR or BCR encoding DNA, and to exclude efforts to correct non-uniformamplification of pseudogenes and orphons (i.e., TCR or BCR Vregion-encoding segments that have been duplicated onto otherchromosomes).

In the human IGH locus, for instance, the ImmunoGeneTics (IMGT) database(M.-P. LeFranc, Université Montpellier, Montpellier, France;www.imgt.org) annotates 165 V segment genes, of which 26 are orphons onother chromosomes and 139 are in the IGH locus at chromosome 14. Amongthe 139 V segments within the IGH locus, 51 have at least one functionalallele, while 6 are ORFs (open-reading frames) which are missing atleast one highly conserved amino-acid residue, and 81 are pseudogenes.Pseudogenes may include V segments that contain an in-frame stop codonwithin the V-segment coding sequence, a frameshift between the startcodon and the CDR3 encoding sequence, one or more repeat-elementinsertions, and deletions of critical regions, such as the first exon orthe RSS. To characterize functional IGH rearrangements in a sample whileavoiding the time and expense of characterizing pseudogenes and/ororphons, it is therefore contemplated to use a subset of the hereindescribed synthetic template oligonucleotides which is designed toinclude only those V segments that participate in a functionalrearrangement to encode a TCR or BCR, without having to synthesize orcalibrate amplification primers and template oligonucleotides specificto the pseudogene sequences. Advantageous efficiencies with respect,inter alia, to time and expense are thus obtained.

TABLE 1 Exemplary Oligonucleotide Primer Set (hsTCRB PCR Primers) SEQ IDName Sequence NO: TRBJ1-1 TTACCTACAACTGTGAGTCTGGTGCCTTGTCC 1631 AAATRBJ1-2 ACCTACAACGGTTAACCTGGTCCCCGAACCGAA 1632 TRBJ1-3ACCTACAACAGTGAGCCAACTTCCCTCTCCAAA 1633 TRBJ1-4CCAAGACAGAGAGCTGGGTTCCACTGCCAAA 1634 TRBJ1-5ACCTAGGATGGAGAGTCGAGTCCCATCACCAAA 1635 TRBJ1-6CTGTCACAGTGAGCCTGGTCCCGTTCCCAAA 1636 TRBJ2-1 CGGTGAGCCGTGTCCCTGGCCCGAA1637 TRBJ2-2 CCAGTACGGTCAGCCTAGAGCCTTCTCCAAA 1638 TRBJ2-3ACTGTCAGCCGGGTGCCTGGGCCAAA 1639 TRBJ2-4 AGAGCCGGGTCCCGGCGCCGAA 1640TRBJ2-5 GGAGCCGCGTGCCTGGCCCGAA 1641 TRBJ2-6 GTCAGCCTGCTGCCGGCCCCGAA 1642TRBJ2-7 GTGAGCCTGGTGCCCGGCCCGAA 1643 TRB2V10-1AACAAAGGAGAAGTCTCAGATGGCTACAG 1644 TRB2V10-2GATAAAGGAGAAGTCCCCGATGGCTATGT 1645 TRB2V10-3GACAAAGGAGAAGTCTCAGATGGCTATAG 1646 TRB2V6-2/3GCCAAAGGAGAGGTCCCTGATGGCTACAA 1647 TRB2V6-8CTCTAGATTAAACACAGAGGATTTCCCAC 1648 TRB2V6-9AAGGAGAAGTCCCCGATGGCTACAATGTA 1649 TRB2V6-5AAGGAGAAGTCCCCAATGGCTACAATGTC 1650 TRB2V6-6GACAAAGGAGAAGTCCCGAATGGCTACAAC 1651 TRB2V6-7GTTCCCAATGGCTACAATGTCTCCAGATC 1652 TRB2V6-1GTCCCCAATGGCTACAATGTCTCCAGATT 1653 TRB2V6-4GTCCCTGATGGTTATAGTGTCTCCAGAGC 1654 TRB2V24-1ATCTCTGATGGATACAGTGTCTCTCGACA 1655 TRB2V25-1TTTCCTCTGAGTCAACAGTCTCCAGAATA 1656 TRB2V27 TCCTGAAGGGTACAAAGTCTCTCGAAAAG1657 TRB2V26 CTCTGAGAGGTATCATGTTTCTTGAAATA 1658 TRB2V28TCCTGAGGGGTACAGTGTCTCTAGAGAGA 1659 TRB2V19 TATAGCTGAAGGGTACAGCGTCTCTCGGG1660 TRB2V4-1 CTGAATGCCCCAACAGCTCTCTCTTAAAC 1661 TRB2V4-2/3CTGAATGCCCCAACAGCTCTCTCTTAAAC 1662 TRB2V2P CCTGAATGCCCTGACAGCTCTCGCTTATA1663 TRB2V3-1 CCTAAATCTCCAGACAAAGCTCACTTAAA 1664 TRB2V3-2CTCACCTGACTCTCCAGACAAAGCTCAT 1665 TRB2V16 TTCAGCTAAGTGCCTCCCAAATTCACCCT1666 TRB2V23-1 GATTCTCATCTCAATGCCCCAAGAACGC 1667 TRB2V18ATTTTCTGCTGAATTTCCCAAAGAGGGCC 1668 TRB2V17 ATTCACAGCTGAAAGACCTAACGGAACGT1669 TRB2V14 TCTTAGCTGAAAGGACTGGAGGGACGTAT 1670 TRB2V2TTCGATGATCAATTCTCAGTTGAAAGGCC 1671 TRB2V12-1 TTGATTCTCAGCACAGATGCCTGATGT1672 TRB2V12-2 GCGATTCTCAGCTGAGAGGCCTGATGG 1673 TRB2V12-3/4TCGATTCTCAGCTAAGATGCCTAATGC 1674 TRB2V12-5TTCTCAGCAGAGATGCCTGATGCAACTTTA 1675 TRB2V7-9GGTTCTCTGCAGAGAGGCCTAAGGGATCT 1676 TRB2V7-8GCTGCCCAGTGATCGCTTCTTTGCAGAAA 1677 TRB2V7-4GGCGGCCCAGTGGTCGGTTCTCTGCAGAG 1678 TRB2V7-6/7ATGATCGGTTCTCTGCAGAGAGGCCTGAGG 1679 TRB2V7-2AGTGATCGCTTCTCTGCAGAGAGGACTGG 1680 TRB2V7-3 GGCTGCCCAACGATCGGTTCTTTGCAGT1681 TRB2V7-1 TCCCCGTGATCGGTTCTCTGCACAGAGGT 1682 TRB2V11-CTAAGGATCGATTTTCTGCAGAGAGGCTC 1683 123 TRB2V13CTGATCGATTCTCAGCTCAACAGTTCAGT 1684 TRB2V5-1TGGTCGATTCTCAGGGCGCCAGTTCTCTA 1685 TRB2V5-3TAATCGATTCTCAGGGCGCCAGTTCCATG 1686 TRB2V5-4TCCTAGATTCTCAGGTCTCCAGTTCCCTA 1687 TRB2V5-8GGAAACTTCCCTCCTAGATTTTCAGGTCG 1688 TRB2V5-5AAGAGGAAACTTCCCTGATCGATTCTCAGC 1689 TRB2V5-6GGCAACTTCCCTGATCGATTCTCAGGTCA 1690 TRB2V9 GTTCCCTGACTTGCACTCTGAACTAAAC1691 TRB2V15 GCCGAACACTTCTTTCTGCTTTCTTGAC 1692 TRB2V30GACCCCAGGACCGGCAGTTCATCCTGAGT 1693 TRB2V20-1ATGCAAGCCTGACCTTGTCCACTCTGACA 1694 TRB2V29-1CATCAGCCGCCCAAACCTAACATTCTCAA 1695

In certain embodiments, the V-segment and J-segment oligonucleotideprimers as described herein are designed to include nucleotide sequencessuch that adequate information is present within the sequence of anamplification product of a rearranged adaptive immune receptor (TCR orIg) gene to identify uniquely both the specific V and the specific Jgenes that give rise to the amplification product in the rearrangedadaptive immune receptor locus (e.g., at least 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 base pairs of sequenceupstream of the V gene recombination signal sequence (RSS), preferablyat least about 22, 24, 26, 28, 30, 32, 34, 35, 36, 37, 38, 39 or 40 basepairs of sequence upstream of the V gene recombination signal sequence(RSS), and in certain preferred embodiments greater than 40 base pairsof sequence upstream of the V gene recombination signal sequence (RSS),and at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19 or 20 base pairs downstream of the J gene RSS, preferably atleast about 22, 24, 26, 28 or 30 base pairs downstream of the J geneRSS, and in certain preferred embodiments greater than 30 base pairsdownstream of the J gene RSS).

This feature stands in contrast to oligonucleotide primers described inthe art for amplification of TCR-encoding or Ig-encoding gene sequences,which rely primarily on the amplification reaction merely for detectionof presence or absence of products of appropriate sizes for V and Jsegments (e.g., the presence in PCR reaction products of an amplicon ofa particular size indicates presence of a V or J segment but fails toprovide the sequence of the amplified PCR product and hence fails toconfirm its identity, such as the common practice of spectratyping).

Oligonucleotides (e.g., primers) can be prepared by any suitable method,including direct chemical synthesis by a method such as thephosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90-99;the phosphodiester method of Brown et al., 1979, Meth. Enzymol.68:109-151; the diethylphosphoramidite method of Beaucage et al., 1981,Tetrahedron Lett. 22:1859-1862; and the solid support method of U.S.Pat. No. 4,458,066, each incorporated herein by reference. A review ofsynthesis methods of conjugates of oligonucleotides and modifiednucleotides is provided in Goodchild, 1990, Bioconjugate Chemistry 1(3):165-187, incorporated herein by reference.

The term “primer,” as used herein, refers to an oligonucleotide capableof acting as a point of initiation of DNA synthesis under suitableconditions. Such conditions include those in which synthesis of a primerextension product complementary to a nucleic acid strand is induced inthe presence of four different nucleoside triphosphates and an agent forextension (e.g., a DNA polymerase or reverse transcriptase) in anappropriate buffer and at a suitable temperature.

A primer is preferably a single-stranded DNA. The appropriate length ofa primer depends on the intended use of the primer but typically rangesfrom 6 to 50 nucleotides, or in certain embodiments, from 15-35nucleotides. Short primer molecules generally require coolertemperatures to form sufficiently stable hybrid complexes with thetemplate. A primer need not reflect the exact sequence of the templatenucleic acid, but must be sufficiently complementary to hybridize withthe template. The design of suitable primers for the amplification of agiven target sequence is well known in the art and described in theliterature cited herein.

As described herein, primers can incorporate additional features whichallow for the detection or immobilization of the primer but do not alterthe basic property of the primer, that of acting as a point ofinitiation of DNA synthesis. For example, primers may contain anadditional nucleic acid sequence at the 5′ end which does not hybridizeto the target nucleic acid, but which facilitates cloning, detection, orsequencing of the amplified product. The region of the primer which issufficiently complementary to the template to hybridize is referred toherein as the hybridizing region.

As used herein, a primer is “specific,” for a target sequence if, whenused in an amplification reaction under sufficiently stringentconditions, the primer hybridizes primarily to the target nucleic acid.Typically, a primer is specific for a target sequence if theprimer-target duplex stability is greater than the stability of a duplexformed between the primer and any other sequence found in the sample.One of skill in the art will recognize that various factors, such assalt conditions as well as base composition of the primer and thelocation of the mismatches, will affect the specificity of the primer,and that routine experimental confirmation of the primer specificitywill be needed in many cases. Hybridization conditions can be chosenunder which the primer can form stable duplexes only with a targetsequence. Thus, the use of target-specific primers under suitablystringent amplification conditions enables the selective amplificationof those target sequences which contain the target primer binding sites.

In particular embodiments, primers for use in the methods describedherein comprise or consist of a nucleic acid of at least about 15nucleotides long that has the same sequence as, or is complementary to,a 15 nucleotide long contiguous sequence of the target V or J segment.Longer primers, e.g., those of about 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, or50, nucleotides long that have the same sequence as, or sequencecomplementary to, a contiguous sequence of the target V or J segment,will also be of use in certain embodiments. All intermediate lengths ofthe aforementioned primers are contemplated for use herein. As would berecognized by the skilled person, the primers may have additionalsequence added (e.g., nucleotides that may not be the same as orcomplementary to the target V or J segment), such as restriction enzymerecognition sites, adaptor sequences for sequencing, bar code sequences,and the like (see e.g., primer sequences provided herein and in thesequence listing). Therefore, the length of the primers may be longer,such as 55, 56, 57, 58, 59, 60, 65, 70, 75, nucleotides in length ormore, depending on the specific use or need. For example, in oneembodiment, the forward and reverse primers are both modified at the 5′end with the universal forward primer sequence compatible with a DNAsequencer.

Also contemplated for use in certain embodiments are adaptive immunereceptor V-segment or J-segment oligonucleotide primer variants that mayshare a high degree of sequence identity to the oligonucleotide primersfor which nucleotide sequences are presented herein, including those setforth in the Sequence Listing. Thus, in these and related embodiments,adaptive immune receptor V-segment or J-segment oligonucleotide primervariants may have substantial identity to the adaptive immune receptorV-segment or J-segment oligonucleotide primer sequences disclosedherein, for example, such oligonucleotide primer variants may compriseat least 70% sequence identity, preferably at least 75%, 80%, 85%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% or higher sequenceidentity compared to a reference polynucleotide sequence such as theoligonucleotide primer sequences disclosed herein, using the methodsdescribed herein (e.g., BLAST analysis using standard parameters). Oneskilled in this art will recognize that these values can beappropriately adjusted to determine corresponding ability of anoligonucleotide primer variant to anneal to an adaptive immune receptorsegment-encoding polynucleotide by taking into account codon degeneracy,reading frame positioning and the like.

Typically, oligonucleotide primer variants will contain one or moresubstitutions, additions, deletions and/or insertions, preferably suchthat the annealing ability of the variant oligonucleotide is notsubstantially diminished relative to that of an adaptive immune receptorV-segment or J-segment oligonucleotide primer sequence that isspecifically set forth herein. As also noted elsewhere herein, inpreferred embodiments adaptive immune receptor V-segment and J-segmentoligonucleotide primers are designed to be capable of amplifying arearranged TCR or IGH sequence that includes the coding region for CDR3.

According to certain embodiments contemplated herein, the primers foruse in the multiplex PCR methods of the present disclosure may befunctionally blocked to prevent non-specific priming of non-T or B cellsequences. For example, the primers may be blocked with chemicalmodifications as described in U.S. patent application publicationUS2010/0167353. According to certain herein disclosed embodiments, theuse of such blocked primers in the present multiplex PCR reactionsinvolves primers that may have an inactive configuration wherein DNAreplication (i.e., primer extension) is blocked, and an activatedconfiguration wherein DNA replication proceeds. The inactiveconfiguration of the primer is present when the primer is eithersingle-stranded, or when the primer is specifically hybridized to thetarget DNA sequence of interest but primer extension remains blocked bya chemical moiety that is linked at or near to the 3′ end of the primer.

The activated configuration of the primer is present when the primer ishybridized to the target nucleic acid sequence of interest and issubsequently acted upon by RNase H or another cleaving agent to removethe 3′ blocking group, thereby allowing an enzyme (e.g., a DNApolymerase) to catalyze primer extension in an amplification reaction.Without wishing to be bound by theory, it is believed that the kineticsof the hybridization of such primers are akin to a second orderreaction, and are therefore a function of the T cell or B cell genesequence concentration in the mixture. Blocked primers minimizenon-specific reactions by requiring hybridization to the target followedby cleavage before primer extension can proceed. If a primer hybridizesincorrectly to a sequence that is related to the desired target sequencebut which differs by having one or more non-complementary nucleotidesthat result in base-pairing mismatches, cleavage of the primer isinhibited, especially when there is a mismatch that lies at or near thecleavage site. This strategy to improve the fidelity of amplificationreduces the frequency of false priming at such locations, and therebyincreases the specificity of the reaction. As would be recognized by theskilled person, reaction conditions, particularly the concentration ofRNase H and the time allowed for hybridization and extension in eachcycle, can be optimized to maximize the difference in cleavageefficiencies between highly efficient cleavage of the primer when it iscorrectly hybridized to its true target sequence, and poor cleavage ofthe primer when there is a mismatch between the primer and the templatesequence to which it may be incompletely annealed.

As described in US2010/0167353, a number of blocking groups are known inthe art that can be placed at or near the 3′ end of the oligonucleotide(e.g., a primer) to prevent extension. A primer or other oligonucleotidemay be modified at the 3′-terminal nucleotide to prevent or inhibitinitiation of DNA synthesis by, for example, the addition of a 3′deoxyribonucleotide residue (e.g., cordycepin), a2′,3′-dideoxyribonucleotide residue, non-nucleotide linkages oralkane-diol modifications (U.S. Pat. No. 5,554,516). Alkane diolmodifications which can be used to inhibit or block primer extensionhave also been described by Wilk et al., (1990 Nucleic Acids Res. 18(8):2065), and by Arnold et al. (U.S. Pat. No. 6,031,091). Additionalexamples of suitable blocking groups include 3′ hydroxyl substitutions(e.g., 3′-phosphate, 3′-triphosphate or 3′-phosphate diesters withalcohols such as 3-hydroxypropyl), 2′3′-cyclic phosphate, 2′ hydroxylsubstitutions of a terminal RNA base (e.g., phosphate or stericallybulky groups such as triisopropyl silyl (TIPS) or tert-butyl dimethylsilyl (TBDMS)). 2′-alkyl silyl groups such as TIPS and TBDMS substitutedat the 3′-end of an oligonucleotide are described by Laikhter et al.,U.S. patent application Ser. No. 11/686,894, which is incorporatedherein by reference. Bulky substituents can also be incorporated on thebase of the 3′-terminal residue of the oligonucleotide to block primerextension.

In certain embodiments, the oligonucleotide may comprise a cleavagedomain that is located upstream (e.g., 5′ to) of the blocking group usedto inhibit primer extension. As examples, the cleavage domain may be anRNase H cleavage domain, or the cleavage domain may be an RNase H2cleavage domain comprising a single RNA residue, or the oligonucleotidemay comprise replacement of the RNA base with one or more alternativenucleosides. Additional illustrative cleavage domains are described inUS2010/0167353.

Thus, a multiplex PCR system may use 40, 45, 50, 55, 60, 65, 70, 75, 80,85, or more forward primers, wherein each forward primer iscomplementary to a single functional TCR or Ig V segment or a smallfamily of functional TCR or Ig V segments, e.g., a TCR VP segment, (seee.g., the TCRBV primers as shown in Table 1, SEQ ID NOS:1644-1695), and,for example, thirteen reverse primers, each specific to a TCR or Ig Jsegment, such as TCR Jβ segment (see e.g., TCRBJ primers in Table 1, SEQID NOS:1631-1643). In another embodiment, a multiplex PCR reaction mayuse four forward primers each specific to one or more functional TCRγ Vsegment and four reverse primers each specific for one or more TCRγ Jsegments. In another embodiment, a multiplex PCR reaction may use 84forward primers each specific to one or more functional V segments andsix reverse primers each specific for one or more J segments.

Thermal cycling conditions may follow methods of those skilled in theart. For example, using a PCR Express™ thermal cycler (Hybaid, Ashford,UK), the following cycling conditions may be used: 1 cycle at 95° C. for15 minutes, 25 to 40 cycles at 94° C. for 30 seconds, 59° C. for 30seconds and 72° C. for 1 minute, followed by one cycle at 72° C. for 10minutes. As will be recognized by the skilled person, thermal cyclingconditions may be optimized, for example, by modifying annealingtemperatures, annealing times, number of cycles and extension times. Aswould be recognized by the skilled person, the amount of primer andother PCR reagents used, as well as PCR parameters (e.g., annealingtemperature, extension times and cycle numbers), may be optimized toachieve desired PCR amplification efficiency.

Alternatively, in certain related embodiments also contemplated herein,“digital PCR” methods can be used to quantitate the number of targetgenomes in a sample, without the need for a standard curve. In digitalPCR, the PCR reaction for a single sample is performed in a multitude ofmore than 100 microcells or droplets, such that each droplet eitheramplifies (e.g., generation of an amplification product providesevidence of the presence of at least one template molecule in themicrocell or droplet) or fails to amplify (evidence that the templatewas not present in a given microcell or droplet). By simply counting thenumber of positive microcells, it is possible directly to count thenumber of target genomes that are present in an input sample.

Digital PCR methods typically use an endpoint readout, rather than aconventional quantitative PCR signal that is measured after each cyclein the thermal cycling reaction (see, e.g., Pekin et al., 2011 Lab. Chip11(13):2156; Zhong et al., 2011 Lab. Chip 11(13):2167; Tewhey et al.,2009 Nature Biotechnol. 27:1025; 2010 Nature Biotechnol. 28:178;Vogelstein and Kinzler, 1999 Proc. Natl. Acad. Sci. USA 96:9236-41; Pohland Shih, 2004 Expert Rev. Mol. Diagn. 4(1);41-7, 2004). Compared withtraditional PCR, dPCR has the following advantages: (1) there is no needto rely on references or standards, (2) desired precision may beachieved by increasing the total number of PCR replicates, (3) it ishighly tolerant to inhibitors, (4) it is capable of analyzing complexmixtures, and (5) it provides a linear response to the number of copiespresent in a sample to allow for small change in the copy number to bedetected. Accordingly, any of the herein described compositions (e.g.,template compositions and adaptive immune receptor gene-specificoligonucleotide primer sets) and methods may be adapted for use in suchdigital PCR methodology, for example, the ABI QuantStudio™ 12K FlexSystem (Life Technologies, Carlsbad, Calif.), the QX100™ DropletDigital™ PCR system (BioRad, Hercules, Calif.), the QuantaLife™ digitalPCR system (BioRad, Hercules, Calif.) or the RainDance™ microdropletdigital PCR system (RainDance Technologies, Lexington, Mass.).

Adaptors

The herein described template oligonucleotides of general formula (I)also may in certain embodiments comprise first (U1) and second (U2)universal adaptor oligonucleotide sequences, or may lack either or bothof U1 and U2. U1 thus may comprise either nothing or an oligonucleotidehaving a sequence that is selected from (i) a first universal adaptoroligonucleotide sequence, and (ii) a first sequencing platform-specificoligonucleotide sequence that is linked to and positioned 5′ to a firstuniversal adaptor oligonucleotide sequence, and U2 may comprise eithernothing or an oligonucleotide having a sequence that is selected from(i) a second universal adaptor oligonucleotide sequence, and (ii) asecond sequencing platform-specific oligonucleotide sequence that islinked to and positioned 5′ to a second universal adaptoroligonucleotide sequence.

U1 and/or U2 may, for example, comprise universal adaptoroligonucleotide sequences and/or sequencing platform-specificoligonucleotide sequences that are specific to a single-moleculesequencing technology being employed, for example the HiSeg™ orGeneAnalyzer™-2 (GA-2) systems (Illumina, Inc., San Diego, Calif.) oranother suitable sequencing suite of instrumentation, reagents andsoftware. Inclusion of such platform-specific adaptor sequences permitsdirect quantitative sequencing of the presently described templatecomposition, which comprises a plurality of different templateoligonucleotides of general formula (I), using a nucleotide sequencingmethodology such as the HiSeq™ or GA2 or equivalent. This featuretherefore advantageously permits qualitative and quantitativecharacterization of the template composition.

In particular, the ability to sequence all components of the templatecomposition directly allows for verification that each templateoligonucleotide in the plurality of template oligonucleotides is presentin a substantially equimolar amount. For example, a set of the presentlydescribed template oligonucleotides may be generated that have universaladaptor sequences at both ends, so that the adaptor sequences can beused to further incorporate sequencing platform-specificoligonucleotides at each end of each template.

Without wishing to be bound by theory, platform-specificoligonucleotides may be added onto the ends of such modified templatesusing 5′ (5 ‘-platform sequence-universal adaptor-1 sequence-3’) and 3′(5′-platform sequence-universal adaptor-2 sequence-3′) oligonucleotidesin as little as two cycles of denaturation, annealing and extension, sothat the relative representation in the template composition of each ofthe component template oligonucleotides is not quantitatively altered.Unique identifier sequences (e.g., barcode sequences B comprising uniqueV and B oligonucleotide sequences that are associated with and thusidentify, respectively, individual V and J regions, as described herein)are placed adjacent to the adaptor sequences, thus permittingquantitative sequencing in short sequence reads, in order tocharacterize the template population by the criterion of the relativeamount of each unique template sequence that is present.

Where such direct quantitative sequencing indicates that one or moreparticular oligonucleotides may be over- or underrepresented in apreparation of the template composition, adjustment of the templatecomposition can be made accordingly to obtain a template composition inwhich all oligonucleotides are present in substantially equimolaramounts. The template composition in which all oligonucleotides arepresent in substantially equimolar amounts may then be used as acalibration standard for amplification primer sets, such as in thepresently disclosed methods for determining and correcting non-uniformamplification potential among members of a primer set.

In addition to adaptor sequences described in the Examples and includedin the exemplary template sequences in the Sequence Listing (e.g., atthe 5′ and 3′ ends of SEQ ID NOS:1-1630), other oligonucleotidesequences that may be used as universal adaptor sequences will be knownto those familiar with the art in view of the present disclosure,including selection of adaptor oligonucleotide sequences that aredistinct from sequences found in other portions of the herein describedtemplates. Non-limiting examples of additional adaptor sequences areshown in Table 2 and set forth in SEQ ID NOS:1710-1731.

TABLE 2 Exemplary Adaptor Sequences SEQ ID Adaptor (primer) nameSequence NO: T7 Promotor AATACGACTCACTATAGG 1710 T7 TerminatorGCTAGTTATTGCTCAGCGG 1711 T3 ATTAACCCTCACTAAAGG 1712 SP6GATTTAGGTGACACTATAG 1713 M13F(−21) TGTAAAACGACGGCCAGT 1714 M13F(−40)GTTTTCCCAGTCACGAC 1715 M13R Reverse CAGGAAACAGCTATGACC 1716 AOX1 ForwardGACTGGTTCCAATTGACAAGC 1717 AOX1 Reverse GCAAATGGCATTCTGACATCC 1718pGEX Forward (GST 5, GGGCTGGCAAGCCACGTTTGGTG 1719 pGEX 5′)pGEX Reverse (GST 3, CCGGGAGCTGCATGTGTCAGAGG 1720 pGEX 3′) BGH ReverseAACTAGAAGGCACAGTCGAGGC 1721 GFP (C′ terminal, CFP, CACTCTCGGCATGGACGAGC1772 YFP or BEP) GFP Reverse TGGTGCAGATGAACTTCAGG 1723 GAGGTTCGACCCCGCCTCGATCC 1724 GAG Reverse TGACACACATTCCACAGGGTC 1725CYC1 Reverse GCGTGAATGTAAGCGTGAC 1726 pFastBacF5′-d (GGATTATTCATACCGTCCCA)-3′ 1727 pFastBacR5′-d (CAAATGTGGTATGGCTGATT)-3′ 1728 pBAD Forward5′-d (ATGCCATAGCATTTTTATCC)-3′ 1729 pBAD Reverse5′-d (GATTTAATCTGTATCAGG)-3′ 1730 CMV-Forward5′-d (CGCAAATGGGCGGTAGGCGTG)-3′ 1731

Barcodes

As described herein, certain embodiments contemplate designing thetemplate oligonucleotide sequences to contain short signature sequencesthat permit unambiguous identification of the template sequence, andhence of at least one primer responsible for amplifying that template,without having to sequence the entire amplification product. In theherein described template oligonucleotides of general formula (I), B1,B2, B3, and B4 are each independently either nothing or each comprisesan oligonucleotide B that comprises an oligonucleotide barcode sequenceof 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25,30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700,800, 900 or 1000 or more contiguous nucleotides (including all integervalues therebetween), wherein in each of the plurality of templateoligonucleotide sequences B comprises a unique oligonucleotide sequencethat uniquely identifies, as a paired combination, (i) the unique Voligonucleotide sequence of the template oligonucleotide and (ii) theunique J oligonucleotide sequence of the template oligonucleotide.

Thus, for instance, template oligonucleotides having barcode identifiersequences may permit relatively short amplification product sequencereads, such as barcode sequence reads of no more than 1000, 900, 800,700, 600, 500, 400, 300, 200, 100, 90, 80, 70, 60, 55, 50, 45, 40, 35,30, 25, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4 orfewer nucleotides, followed by matching this barcode sequenceinformation to the associated V and J sequences that are incorporatedinto the template having the barcode as part of the template design. Bythis approach, a large number of amplification products can besimultaneously partially sequenced by high throughput parallelsequencing, to identify primers that are responsible for amplificationbias in a complex primer set.

Exemplary barcodes may comprise a first barcode oligonucleotide of 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 nucleotides that uniquelyidentifies each V polynucleotide in the template and a second barcodeoligonucleotide of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16nucleotides that uniquely identifies each J polynucleotide in thetemplate, to provide barcodes of, respectively, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 or 32nucleotides in length, but these and related embodiments are notintended to be so limited. Barcode oligonucleotides may compriseoligonucleotide sequences of any length, so long as a minimum barcodelength is obtained that precludes occurrence of a given barcode sequencein two or more template oligonucleotides having otherwise distinctsequences (e.g., V and J sequences).

Thus, the minimum barcode length, to avoid such redundancy amongst thebarcodes that are used to uniquely identify different V−J sequencepairings, is X nucleotides, where 4^(X) is greater than the number ofdistinct template species that are to be differentiated on the basis ofhaving non-identical sequences. For example, for the set of 871 templateoligonucleotides set forth herein as SEQ ID NOS:1-871, the minimumbarcode length would be five nucleotides, which would permit atheoretical total of 1024 (i.e., greater than 871) different possiblepentanucleotide sequences. In practice, barcode oligonucleotide sequenceread lengths may be limited only by the sequence read-length limits ofthe nucleotide sequencing instrument to be employed. For certainembodiments, different barcode oligonucleotides that will distinguishindividual species of template oligonucleotides should have at least twonucleotide mismatches (e.g., a minimum hamming distance of 2) whenaligned to maximize the number of nucleotides that match at particularpositions in the barcode oligonucleotide sequences.

In preferred embodiments, for each distinct template oligonucleotidespecies having a unique sequence within the template composition ofgeneral formula (I), B 1, B2, B3, and B4 will be identical.

The skilled artisan will be familiar with the design, synthesis, andincorporation into a larger oligonucleotide or polynucleotide construct,of oligonucleotide barcode sequences of, for instance, at least 3, 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 30, 35, 40, 45, 50, 55, 60, 70, 80, 90, 100, 200, 300, 300, 500 ormore contiguous nucleotides, including all integer values therebetween.For non-limiting examples of the design and implementation ofoligonucleotide barcode sequence identification strategies, see, e.g.,de Carcer et al., 2011 Adv. Env. Microhiol. 77:6310; Parameswaran etal., 2007 Nucl. Ac. Res. 35(19):330; Roh et al., 2010 Trends Biotechnol.28:291.

Typically, barcodes are placed in templates at locations where they arenot found naturally, i.e., barcodes comprise nucleotide sequences thatare distinct from any naturally occurring oligonucleotide sequences thatmay be found in the vicinity of the sequences adjacent to which thebarcodes are situated (e.g., V and/or J sequences). Such barcodesequences may be included, according to certain embodiments describedherein, as elements B1, B2 and/or B3 of the presently disclosed templateoligonucleotide of general formula (I). Accordingly, certain of theherein described template oligonucleotides of general formula (I) mayalso in certain embodiments comprise one, two or all three of barcodesB1, B2 and B3, while in certain other embodiments some or all of thesebarcodes may be absent. In certain embodiments all barcode sequenceswill have identical or similar GC content (e.g., differing in GC contentby no more than 20%, or by no more than 19, 18, 17, 16, 15, 14, 13, 12,11 or 10%).

In the template compositions according to certain herein disclosedembodiments the barcode-containing element B (e.g., B1, B2, B3, and/orB4) comprises the oligonucleotide sequence that uniquely identifies asingle paired V−J combination. Optionally and in certain embodiments thebarcode-containing element B may also include a random nucleotide, or arandom polynucleotide sequence of at least 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, 50, 55, 60, 70, 80,90, 100, 200, 300, 300, 500 or more contiguous nucleotides, situatedupstream and/or downstream of the specific barcode sequence thatuniquely identifies each specific paired V−J combination. When presentboth upstream and downstream of the specific barcode sequence, therandom nucleotide or random polynucleotide sequence are independent ofone another, that is, they may but need not comprise the same nucleotideor the same polynucleotide sequence.

Restriction Enzyme Sites

According to certain embodiments disclosed herein, the templateoligonucleotide may comprise a restriction endonuclease (RE) recognitionsite that is situated between the V and J sequences and does not occurelsewhere in the template oligonucleotide sequence. The RE recognitionsite may optionally be adjacent to a barcode site that identifies the Vregion sequence. The RE site may be included for any of a number ofpurposes, including without limitation as a structural feature that maybe exploited to destroy templates selectively by contacting them withthe appropriate restriction enzyme. It may be desirable to degrade thepresent template oligonucleotides selectively by contacting them with asuitable RE, for example, to remove template oligonucleotides from othercompositions into which they may have been deliberately or accidentallyintroduced. Alternatively, the RE site may be usefully exploited in thecourse of sequencing template oligonucleotides in the templatecomposition, and/or as a positional sequence marker in a templateoligonucleotide sequence regardless of whether or not it is cleaved witha restriction enzyme. An exemplary RE site is the oligonucleotide motifGTCGAC, which is recognized by the restriction enzyme Sal I. A largenumber of additional restriction enzymes and their respective RErecognition site sequences are known in the art and are availablecommercially (e.g., New England Biolabs, Beverly, Mass.). These include,for example, EcoRI (GAATTC) and SphI (GCATGC). Those familiar with theart will appreciate that any of a variety of such RE recognition sitesmay be incorporated into particular embodiments of the presentlydisclosed template oligonucleotides.

Sequencing

Sequencing may be performed using any of a variety of available highthroughput single molecule sequencing machines and systems. Illustrativesequence systems include sequence-by-synthesis systems such as theIllumina Genome Analyzer and associated instruments (Illumina, Inc., SanDiego, Calif.), Helicos Genetic Analysis System (Helicos BioSciencesCorp., Cambridge, Mass.), Pacific Biosciences PacBio RS (PacificBiosciences, Menlo Park, Calif.), or other systems having similarcapabilities. Sequencing is achieved using a set of sequencingoligonucleotides that hybridize to a defined region within the amplifiedDNA molecules. The sequencing oligonucleotides are designed such thatthe V- and J-encoding gene segments can be uniquely identified by thesequences that are generated, based on the present disclosure and inview of known adaptive immune receptor gene sequences that appear inpublicly available databases. See, e.g., U.S. application Ser. No.13/217,126; U.S. application Ser. No. 12/794,507; PCT/US2011/026373; orPCT/US2011/049012. Exemplary TCRB J-region sequencing primers are setforth in Table 3:

TABLE 3 TCRBJ Sequencing Primers SEQ ID PRIMER SEQUENCE NO: >Jseq1-1ACAACTGTGAGTCTGGTGCCTTGTCCAAAGAAA 1696 >Jseq1-2ACAACGGTTAACCTGGTCCCCGAACCGAAGGTG 1697 >Jseq1-3ACAACAGTGAGCCAACTTCCCTCTCCAAAATAT 1698 >Jseq1-4AAGACAGAGAGCTGGGTTCCACTGCCAAAAAAC 1699 >Jseq1-5AGGATGGAGAGTCGAGTCCCATCACCAAAATGC 1700 >Jseq1-6GTCACAGTGAGCCTGGTCCCGTTCCCAAAGTGG 1701 >Jseq2-1AGCACGGTGAGCCGTGTCCCTGGCCCGAAGAAC 1702 >Jseq2-2AGTACGGTCAGCCTAGAGCCTTCTCCAAAAAAC 1703 >Jseq2-3AGCACTGTCAGCCGGGTGCCTGGGCCAAAATAC 1704 >Jseq2-4AGCACTGAGAGCCGGGTCCCGGCGCCGAAGTAC 1705 >Jseq2-5AGCACCAGGAGCCGCGTGCCTGGCCCGAAGTAC 1706 >Jseq2-6AGCACGGTCAGCCTGCTGCCGGCCCCGAAAGTC 1707 >Jseq2-7GTGACCGTGAGCCTGGTGCCCGGCCCGAAGTAC 1708

The term “gene” means the segment of DNA involved in producing apolypeptide chain such as all or a portion of a TCR or Ig polypeptide(e.g., a CDR3-containing polypeptide); it includes regions preceding andfollowing the coding region “leader and trailer” as well as interveningsequences (introns) between individual coding segments (exons), and mayalso include regulatory elements (e.g., promoters, enhancers, repressorbinding sites and the like), and may also include recombination signalsequences (RSSs) as described herein.

The nucleic acids of the present embodiments, also referred to herein aspolynucleotides, may be in the form of RNA or in the form of DNA, whichDNA includes cDNA, genomic DNA, and synthetic DNA. The DNA may bedouble-stranded or single-stranded, and if single stranded may be thecoding strand or non-coding (anti-sense) strand. A coding sequence whichencodes a TCR or an immunoglobulin or a region thereof (e.g., a Vregion, a D segment, a J region, a C region, etc.) for use according tothe present embodiments may be identical to the coding sequence known inthe art for any given TCR or immunoglobulin gene regions or polypeptidedomains (e.g., V-region domains, CDR3 domains, etc.), or may be adifferent coding sequence, which, as a result of the redundancy ordegeneracy of the genetic code, encodes the same TCR or immunoglobulinregion or polypeptide.

In certain embodiments, the amplified J-region encoding gene segmentsmay each have a unique sequence-defined identifier tag of 2, 3, 4, 5, 6,7, 8, 9, 10 or about 15, 20 or more nucleotides, situated at a definedposition relative to a RSS site. For example, a four-base tag may beused, in the Jβ-region encoding segment of amplified TCRβ CDR3-encodingregions, at positions+11 through +14 downstream from the RSS site.However, these and related embodiments need not be so limited and alsocontemplate other relatively short nucleotide sequence-definedidentifier tags that may be detected in J-region encoding gene segmentsand defined based on their positions relative to an RSS site. These mayvary between different adaptive immune receptor encoding loci.

The recombination signal sequence (RSS) consists of two conservedsequences (heptamer, 5′-CACAGTG-3′, and nonamer, 5′-ACAAAAACC-3′),separated by a spacer of either 12+/−1 bp (“12-signal”) or 23+/−1 bp(“23-signal”). A number of nucleotide positions have been identified asimportant for recombination including the CA dinucleotide at positionone and two of the heptamer, and a C at heptamer position three has alsobeen shown to be strongly preferred as well as an A nucleotide atpositions 5, 6, 7 of the nonamer. (Ramsden et. al 1994; Akamatsu et. al.1994; Hesse et. al. 1989). Mutations of other nucleotides have minimalor inconsistent effects. The spacer, although more variable, also has animpact on recombination, and single-nucleotide replacements have beenshown to significantly impact recombination efficiency (Fanning et. al.1996, Larijani et. al 1999; Nadel et. al. 1998). Criteria have beendescribed for identifying RSS polynucleotide sequences havingsignificantly different recombination efficiencies (Ramsden et. al 1994;Akamatsu et. al. 1994; Hesse et. al. 1989 and Cowell et. al. 1994).Accordingly, the sequencing oligonucleotides may hybridize adjacent to afour base tag within the amplified J-encoding gene segments atpositions+11 through+14 downstream of the RSS site. For example,sequencing oligonucleotides for TCRB may be designed to anneal to aconsensus nucleotide motif observed just downstream of this “tag”, sothat the first four bases of a sequence read will uniquely identify theJ-encoding gene segment (see, e.g., WO/2012/027503).

The average length of the CDR3-encoding region, for the TCR, defined asthe nucleotides encoding the TCR polypeptide between the secondconserved cysteine of the V segment and the conserved phenylalanine ofthe J segment, is 35+/−3 nucleotides. Accordingly and in certainembodiments, PCR amplification using V-segment oligonucleotide primerswith J-segment oligonucleotide primers that start from the J segment tagof a particular TCR or IgH J region (e.g., TCR Jβ, TCR Jγ or IgH JH asdescribed herein) will nearly always capture the complete V-D-J junctionin a 50 base pair read. The average length of the IgH CDR3 region,defined as the nucleotides between the conserved cysteine in the Vsegment and the conserved phenylalanine in the J segment, is lessconstrained than at the TCRβ locus, but will typically be between about10 and about 70 nucleotides. Accordingly and in certain embodiments, PCRamplification using V-segment oligonucleotide primers with J-segmentoligonucleotide primers that start from the IgH J segment tag willcapture the complete V-D-J junction in a 100 base pair read.

PCR primers that anneal to and support polynucleotide extension onmismatched template sequences are referred to as promiscuous primers. Incertain embodiments, the TCR and Ig J-segment reverse PCR primers may bedesigned to minimize overlap with the sequencing oligonucleotides, inorder to minimize promiscuous priming in the context of multiplex PCR.In one embodiment, the TCR and Ig J-segment reverse primers may beanchored at the 3′ end by annealing to the consensus splice site motif,with minimal overlap of the sequencing primers. Generally, the TCR andIg V and J-segment primers may be selected to operate in PCR atconsistent annealing temperatures using known sequence/primer design andanalysis programs under default parameters.

For the sequencing reaction, the exemplary IGHJ sequencing primersextend three nucleotides across the conserved CAG sequences as describedin WO/2012/027503.

Samples

The subject or biological source, from which a test biological samplemay be obtained, may be a human or non-human animal, or a transgenic orcloned or tissue-engineered (including through the use of stem cells)organism. In certain preferred embodiments of the invention, the subjector biological source may be known to have, or may be suspected of havingor being at risk for having, a circulating or solid tumor or othermalignant condition, or an autoimmune disease, or an inflammatorycondition, and in certain preferred embodiments of the invention thesubject or biological source may be known to be free of a risk orpresence of such disease.

Certain preferred embodiments contemplate a subject or biological sourcethat is a human subject such as a patient that has been diagnosed ashaving or being at risk for developing or acquiring cancer according toart-accepted clinical diagnostic criteria, such as those of the U.S.National Cancer Institute (Bethesda, Md., USA) or as described inDeVita, Hellman, and Rosenberg's Cancer: Principles and Practice ofOncology (2008, Lippincott, Williams and Wilkins, Philadelphia/Ovid,N.Y.); Pizzo and Poplack, Principles and Practice of Pediatric Oncology(Fourth edition, 2001, Lippincott, Williams and Wilkins,Philadelphia/Ovid, N.Y.); and Vogelstein and Kinzler, The Genetic Basisof Human Cancer (Second edition, 2002, McGraw Hill Professional, NewYork); certain embodiments contemplate a human subject that is known tobe free of a risk for having, developing or acquiring cancer by suchcriteria.

Certain other embodiments contemplate a non-human subject or biologicalsource, for example a non-human primate such as a macaque, chimpanzee,gorilla, vervet, orangutan, baboon or other non-human primate, includingsuch non-human subjects that may be known to the art as preclinicalmodels, including preclinical models for solid tumors and/or othercancers. Certain other embodiments contemplate a non-human subject thatis a mammal, for example, a mouse, rat, rabbit, pig, sheep, horse,bovine, goat, gerbil, hamster, guinea pig or other mammal; many suchmammals may be subjects that are known to the art as preclinical modelsfor certain diseases or disorders, including circulating or solid tumorsand/or other cancers (e.g., Talmadge et al., 2007 Am. J. Pathol.170:793; Kerbel, 2003 Canc. Biol. Therap. 2(4 Suppl 1):5134; Man et al.,2007 Canc. Met. Rev. 26:737; Cespedes et al., 2006 Clin. Transl. Oncol.8:318). The range of embodiments is not intended to be so limited,however, such that there are also contemplated other embodiments inwhich the subject or biological source may be a non-mammalianvertebrate, for example, another higher vertebrate, or an avian,amphibian or reptilian species, or another subject or biological source.

Biological samples may be provided by obtaining a blood sample, biopsyspecimen, tissue explant, organ culture, biological fluid or any othertissue or cell preparation from a subject or a biological source.Preferably the sample comprises DNA from lymphoid cells of the subjector biological source, which, by way of illustration and not limitation,may contain rearranged DNA at one or more TCR or BCR loci. In certainembodiments a test biological sample may be obtained from a solid tissue(e.g., a solid tumor), for example by surgical resection, needle biopsyor other means for obtaining a test biological sample that contains amixture of cells.

According to certain embodiments, it may be desirable to isolatelymphoid cells (e.g., T cells and/or B cells) according to any of alarge number of established methodologies, where isolated lymphoid cellsare those that have been removed or separated from the tissue,environment or milieu in which they naturally occur. B cells and T cellscan thus be obtained from a biological sample, such as from a variety oftissue and biological fluid samples including bone marrow, thymus, lymphglands, lymph nodes, peripheral tissues and blood, but peripheral bloodis most easily accessed. Any peripheral tissue can be sampled for thepresence of B and T cells and is therefore contemplated for use in themethods described herein. Tissues and biological fluids from whichadaptive immune cells, may be obtained include, but are not limited toskin, epithelial tissues, colon, spleen, a mucosal secretion, oralmucosa, intestinal mucosa, vaginal mucosa or a vaginal secretion,cervical tissue, ganglia, saliva, cerebrospinal fluid (CSF), bonemarrow, cord blood, serum, serosal fluid, plasma, lymph, urine, ascitesfluid, pleural fluid, pericardial fluid, peritoneal fluid, abdominalfluid, culture medium, conditioned culture medium or lavage fluid. Incertain embodiments, adaptive immune cells may be isolated from anapheresis sample. Peripheral blood samples may be obtained by phlebotomyfrom subjects. Peripheral blood mononuclear cells (PBMC) are isolated bytechniques known to those of skill in the art, e.g., by Ficoll-Hypaque®density gradient separation. In certain embodiments, whole PBMCs areused for analysis.

For nucleic acid extraction, total genomic DNA may be extracted fromcells using methods known in the art and/or commercially available kits,e.g., by using the QIAamp® DNA blood Mini Kit (QIAGEN®). The approximatemass of a single haploid genome is 3 pg. Preferably, at least 100,000 to200,000 cells are used for analysis, i.e., about 0.6 to 1.2 μg DNA fromdiploid T or B cells. Using PBMCs as a source, the number of T cells canbe estimated to be about 30% of total cells. The number of B cells canalso be estimated to be about 30% of total cells in a PBMC preparation.

The Ig and TCR gene loci contain many different variable (V), diversity(D), and joining (J) gene segments, which are subjected to rearrangementprocesses during early lymphoid differentiation. Ig and TCR V, D and Jgene segment sequences are known in the art and are available in publicdatabases such as GENBANK. The V-D-J rearrangements are mediated via arecombinase enzyme complex in which the RAG1 and RAG2 proteins play akey role by recognizing and cutting the DNA at the recombination signalsequences (RSS), which are located downstream of the V gene segments, atboth sides of the D gene segments, and upstream of the J gene segments.Inappropriate RSS reduce or even completely prevent rearrangement. Therecombination signal sequence (RSS) includes two consensus sequences(heptamer, 5′-CACAGTG-3′, and nonamer, 5′-ACAAAAACC-3′), separated by aspacer of either 12+/−1 bp (“12-signal”) or 23+/−1 bp (“23-signal”). Atthe 3′ end of the V segment and D segment the RSS sequence is heptamer(CACAGTG)-spacer-nonamer (ACAAAAACC). At the 5′ end of the J segment andD segment the RSS sequence is nonamer (GGTTTTTGT)-spacer-heptamer(CACTGTG), with substantial sequence variation in the heptamer andnonamer sequence of each specific gene segment.

A number of nucleotide positions have been identified as important forrecombination including the CA dinucleotide at position one and two ofthe heptamer, and a C at heptamer position three has also been shown tobe strongly preferred as well as an A nucleotide at positions 5, 6, 7 ofthe nonamer. (Ramsden et. al 1994 Nucl. Ac. Res. 22:1785; Akamatsu et.al. 1994 J. Immunol. 153:4520; Hesse et. al. 1989 Genes Dev. 3:1053).Mutations of other nucleotides have minimal or inconsistent effects. Thespacer, although more variable, also has an impact on recombination, andsingle-nucleotide replacements have been shown to significantly impactrecombination efficiency (Fanning et. al. 1996 Cell. Immunol.Immumnopath. 79:1, Larijani et. al 1999 Nucl. Ac. Res. 27:2304; Nadelet. al. 1998 J. Immunol. 161:6068; Nadel et al., 1998 J. Exp. Med.187:1495). Criteria have been described for identifying RSSpolynucleotide sequences having significantly different recombinationefficiencies (Ramsden et. al 1994 Nucl. Ac. Res. 22:1785; Akamatsu et.al. 1994 J. Immunol. 153:4520; Hesse et. al. 1989 Genes Dev. 3:1053, andLee et al., 2003 PLoS 1(1):E1).

The rearrangement process at the Ig heavy chain (IgH), TCR beta (TCRB),and TCR delta (TCRD) genes generally starts with a D to J rearrangementfollowed by a V to D-J rearrangement, while direct V to J rearrangementsoccur at Ig kappa (IgK), Ig lambda (IgL), TCR alpha (TCRA), and TCRgamma (TCRG) genes. The sequences between rearranging gene segments aregenerally deleted in the form of a circular excision product, alsocalled TCR excision circle (TREC) or B cell receptor excision circle(BREC).

The many different combinations of V, D, and J gene segments representthe so-called combinatorial repertoire, which is estimated to be ˜2×10⁶for Ig molecules, ˜3×10⁶ for TCRαβ and ˜5×10³ for TCRγδ molecules. Atthe junction sites of the V, D, and J gene segments, deletion and randominsertion of nucleotides occurs during the rearrangement process,resulting in highly diverse junctional regions, which significantlycontribute to the total repertoire of Ig and TCR molecules, estimated tobe >10¹² possible amino acid sequences.

Mature B-lymphocytes further extend their Ig repertoire upon antigenrecognition in germinal centers via somatic hypermutation, a processleading to affinity maturation of the Ig molecules. The somatichypermutation process focuses on the V- (D-) J exon of IgH and Ig lightchain genes and primarily generates single nucleotide mutations butsometimes also insertions or deletions of nucleotides.Somatically-mutated Ig genes are also typically found in mature B-cellmalignancies.

In certain embodiments described herein, V-segment and J-segment primersmay be employed in a PCR reaction to amplify rearranged TCR or BCRCDR3-encoding DNA regions in a test biological sample, wherein eachfunctional TCR or Ig V-encoding gene segment comprises a V generecombination signal sequence (RSS) and each functional TCR or IgJ-encoding gene segment comprises a J gene RSS. In these and relatedembodiments, each amplified rearranged DNA molecule may comprise (i) atleast about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500,600, 700, 800, 900, 1000 (including all integer values therebetween) ormore contiguous nucleotides of a sense strand of the TCR or IgV-encoding gene segment, with the at least about 10, 20, 30, 40, 50, 60,70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 or morecontiguous nucleotides being situated 5′ to the V gene RSS and/or eachamplified rearranged DNA molecule may comprise (ii) at least about 10,20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500 (including allinteger values therebetween) or more contiguous nucleotides of a sensestrand of the TCR or Ig J-encoding gene segment, with the at least about10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500 or morecontiguous nucleotides being situated 3′ to the J gene RSS.

Amplification Factor Determination

In addition to the use of the presently disclosed template compositionsfor standardizing amplification efficiency of oligonucleotideamplification primer sets as described herein, certain other embodimentscontemplate use of the template composition to determine amplificationfactors for estimating the number of rearranged adaptive immune receptorencoding sequences in a sample. These and related embodiments may finduse to quantify the number of adaptive immune receptor encodingsequences in a DNA sample that has been obtained from lymphoid cells,including lymphoid cells that are present in a mixture of cells thatcomprises cells in which DNA encoding an adaptive immune receptor hasundergone DNA rearrangement, but where the sample also contains DNA fromcells in which no such rearrangement has taken place (e.g., non-lymphoidcells, immature cells, mesenchymal cells, cancer cells, etc.).

The total number of different members of a given class of adaptiveimmune receptors (e.g., TCRs or IGs) in a subject may be estimated bymultiplexed PCR using a comprehensive V−J amplification primer setfollowed by quantitative sequencing of amplification products.Multiplexed amplification and high throughput sequencing of rearrangedTCR and BCR (IG) encoding DNA sequences are described, for example, inRobins et al., 2009 Blood 114, 4099; Robins et al., 2010 Sci. Translat.Med. 2:47ra64; Robins et al., 2011 J. Immunol. Meth.doi:10.1016/j.jim.2011.09.001; Sherwood et al. 2011 Sci. Translat. Med.3:90ra61; U.S. application Ser. No. 13/217,126 (US Pub. No.2012/0058902), U.S. application Ser. No. 12/794,507 (US Pub. No.2010/0330571), WO/2010/151416, WO/2011/106738 (PCT/US2011/026373),WO2012/027503 (PCT/US2011/049012), U.S. Application No. 61/550,311, andU.S. Application No. 61/569,118.

This methodology typically involves sampling DNA from a subpopulation oflymphoid cells, such as lymphoid cells that are present in a bloodsample, which is known also to contain nucleated cells that lackrearranged TCR or IG encoding DNA. The present compositions and methodsmay permit improved accuracy and precision in the determination of thenumber of rearranged TCR and IG encoding DNA molecules in such a sample.As described herein, for instance, by spiking the DNA sample with thepresent template composition, an internal amplification templatestandard is provided for assessing the relative efficiencies across therange of oligonucleotide primers that are present in the multiplexedamplification primer set. By so assessing the amplification products ofthe present artificial template composition, which is added to theamplification reaction in known amounts, an amplification factor (e.g.,a multiplicative, normalizing, scaling or geometric factor, etc.) can bedetermined for the oligonucleotide amplification primer set and can thenbe used to calculate the number of natural DNA templates in the sample.

As another example, these and related embodiments permit quantificationof Minimal Residual Disease (MRD) in lymphoma or leukemia, byquantitative detection of rearranged TCR or IG encoding DNA in samplesobtained from mixed preparations of lymphoid and non-lymphoid cells,including persistent lymphoma or leukemia cells. Prior methods determineMRD as the number of malignant cells that are detectable as a proportionof the total number of cells in a sample. In contrast, the presentmethods permit estimation of the total number of cells in a sample thathave rearranged TCR or IG encoding DNA, so that malignant cells (e.g.,those having a particular TCR or IG rearrangement, such as a clonotype)can be quantified as a proportion of such rearranged cells instead of asa proportion of all cells. By way of non-limiting theory, it is believedthat because the representation of all rearranged cells in a clinicalsample from a subject having or suspected of having MRD is typicallyvery low, the present methods will dramatically improve the sensitivitywith which MRD can be detected, including improving such sensitivity byincreasing the signal-to-noise ratio.

Accordingly certain embodiments thus provide a method for quantifyingrearranged DNA molecules encoding one or a plurality of adaptive immunereceptors in a biological sample that comprises DNA from lymphoid cellsof a subject, each adaptive immune receptor comprising a variable regionand a joining region. Briefly, the method comprises the steps of:

(A) in a multiplexed amplification reaction using the herein describedoligonucleotide amplification primer set that is capable of amplifyingsubstantially all V−J encoding combinations for a given adaptive immunereceptor, amplifying DNA from the sample to which has been added a knownamount of the herein described template composition for standardizingamplification efficiency, to obtain amplification products;

(B) quantitatively sequencing the amplification products of (A) toquantify (i) template amplification products, which are amplificationproducts of the herein described template composition and will beidentifiable because they contain at least one barcode oligonucleotidesequence, and (ii) amplification products of rearranged adaptive immunereceptor encoding DNA sequences in the sample, which will beidentifiable because they contain specific V and J sequences but lack anoligonucleotide barcode sequence;

(C) calculating an amplification factor based on quantitativeinformation obtained in step (B); and

(D) using the amplification factor of (C) to determine, by calculation,the number of unique adaptive immune receptor encoding DNA molecules inthe sample.

Without wishing to be bound by theory, according to these and relatedmethods, the number of rearranged TCR or IG encoding DNA molecules thatare sampled in a multiplexed amplification reaction is measured. To doso, a sequence coverage value, e.g., the number of output sequence readsthat are determined for each input (template) molecule, is determinedand averaged across the entire number of different templateoligonucleotides that are present, to obtain an average sequencecoverage value. By dividing (i) the number of reads that are obtainedfor a given sequence by (ii) the average sequence coverage value, thenumber of rearranged molecules that are present as templates at thestart of the amplification reaction can be calculated.

Thus, for example, to calculate the sequence coverage value, a knownquantity of a set of synthetic molecules of the presently disclosedtemplate composition is added to each PCR amplification, the synthetictemplates having the basic structure of formula (I)5′U-B1-V-B2-R-(B3)-J-B4-U 3′ where each V is a 300 base pair segmenthaving a sequence that matches a TCR or IG V gene sequence and J is a100 base pair segment having a sequence that matches a TCR or IG J gene.B2 is a unique barcode oligonucleotide sequence that uniquely identifieseach VJ pair and that also differentiates amplification products of thesynthetic DNA templates (which will contain the barcode sequence) fromamplification products of naturally occurring biologic template DNAmolecules that are contributed by the lymphoid DNA sample (which willlack the barcode sequence). In this example, B3 of formula (I) isnothing. After PCR amplification and sequencing, the numbers of eachsequenced synthetic molecule (i.e., amplification products containingthe barcode sequence) are counted. The sequence coverage of thesynthetic molecules is then calculated based on the known number ofstarting synthetic template molecules used to spike the amplificationreaction.

For example, a pool of 5000 synthetic, barcode-containing templatemolecules comprising 4-5 copies each of 1100 unique synthetic templateoligonucleotide sequences (representing every possible VJ pair) may beadded to the amplification reaction. If the amplification productsinclude 50,000 sequences that match the synthetic template molecules, asequence coverage value of 10× has been obtained and the amplificationfactor is 10. To estimate the number of natural VDJ-rearranged templatemolecules in the DNA obtained from the sample, the number ofamplification products of the natural templates (i.e., amplificationproducts that lack any barcode sequence) is then divided by theamplification factor. For added accuracy, because in this example the5000 synthetic molecules are a complex pool of 1100 moleculesrepresenting every VJ pair, the amplification factor for every VJ paircan be individually calculated. The amplification factor can then beaveraged across all of the synthetic molecules (FIG. 8). The accuracyand robustness of the method is shown in FIG. 9 and details aredescribed below in Example 5.

In an alternative embodiment, identical to what is described above andbelow in this section, except differing in the use of a subset of thetotal pool of synthetic template molecules is used in a manner resultingin the addition to a sample of not more than 1 copy of a subset ofdistinct template molecules to the sample. Application of Poissonstatistical methods well known to the ordinarily skilled artisan areused to determine the amount of template to add based upon the knownproperties of the pool (e.g., the total number of distinct sequences andthe concentration of template molecules). For example, 200-500 templatemolecules are added to the amplification reaction, such that there is onaverage not more than one copy each of a subset of template moleculespresent in the pool.

Accordingly, in these embodiments the method comprises: (A) amplifyingDNA in a multiplex polymerase chain reaction (PCR) that comprises: (1)DNA from the biological sample that comprises lymphoid cells of thesubject, (2) the template composition of claim 1 in which a known numberof each of the plurality of template oligonucleotides having a uniqueoligonucleotide sequence is present, (3) an oligonucleotideamplification primer set that is capable of amplifying rearranged DNAencoding one or a plurality of adaptive immune receptors in the DNA fromthe biological sample, the primer set comprising: (a) in substantiallyequimolar amounts, a plurality of V-segment oligonucleotide primers thatare each independently capable of specifically hybridizing to at leastone polynucleotide encoding an adaptive immune receptor V-regionpolypeptide or to the complement thereof, wherein each V-segment primercomprises a nucleotide sequence of at least 15 contiguous nucleotidesthat is complementary to at least one functional adaptive immunereceptor V region-encoding gene segment and wherein the plurality ofV-segment primers specifically hybridize to substantially all functionaladaptive immune receptor V region-encoding gene segments that arepresent in the template composition, and (b) in substantially equimolaramounts, a plurality of 0.1-segment oligonucleotide primers that areeach independently capable of specifically hybridizing to at least onepolynucleotide encoding an adaptive immune receptor J-region polypeptideor to the complement thereof, wherein each J-segment primer comprises anucleotide sequence of at least 15 contiguous nucleotides that iscomplementary to at least one functional adaptive immune receptor Jregion-encoding gene segment and wherein the plurality of J-segmentprimers specifically hybridize to substantially all functional adaptiveimmune receptor J region-encoding gene segments that are present in thetemplate composition, wherein the V-segment and J-segmentoligonucleotide primers are capable of promoting amplification in saidmultiplex polymerase chain reaction (PCR) of (i) substantially alltemplate oligonucleotides in the template composition to produce amultiplicity of amplified template DNA molecules, said multiplicity ofamplified template DNA molecules being sufficient to quantify diversityof the template oligonucleotides in the template composition, and (ii)substantially all rearranged DNA molecules encoding adaptive immunereceptors in the biological sample to produce a multiplicity ofamplified rearranged DNA molecules, said multiplicity of amplifiedrearranged DNA molecules being sufficient to quantify diversity of therearranged DNA molecules in the DNA from the biological sample, andwherein each amplified DNA molecule in the multiplicity of amplifiedtemplate DNA molecules and in the multiplicity of amplified rearrangedDNA molecules is less than 1000, 900, 800, 700, 600, 500, 400, 300, 200,100, 90, 80 or 70 nucleotides in length;

(B) quantitatively sequencing all or a sufficient portion of each ofsaid amplified template DNA molecules and each of said amplifiedrearranged DNA molecules to quantify (i) a template product number ofamplified template DNA molecules which contain at least oneoligonucleotide barcode sequence, and (ii) a rearranged product numberof amplified rearranged DNA molecules which lack an oligonucleotidebarcode sequence;

(C) calculating an amplification factor by dividing the template productnumber of (B)(i) by the known number of each of the plurality oftemplate oligonucleotides having a unique oligonucleotide sequence of(A)(2); and

(D) dividing the rearranged product number of (B)(ii) by theamplification factor calculated in (C) to quantify unique adaptiveimmune receptor encoding DNA molecules in the sample.

The contemplated embodiments are not intended to be limited to the abovedescribed method, such that from the present disclosure the skilledperson will appreciate variations that may be employed. An alternativeapproach, for example, may not use the herein described synthetictemplate composition as a spiked-in control template in multiplexed PCRamplification of a DNA sample that contains rearranged lymphoid cell TCRand/or IG encoding DNA as well as non-rearranged DNA. Instead, accordingto one such alternative, to the amplification reaction using V and Jamplification primers may be added a known set of oligonucleotideamplification primers that amplify a distinct, highly conserved genomicsequence region. These genomic control primers may amplify every genomethat is present in the DNA sample regardless of whether or not itcontains rearranged TCR and/or IG encoding sequences, whereas the V andJ primers may amplify products only from genomes with a rearranged VDJregion. The ratio between these two classes of amplification productmolecules permits estimation of the total number of B cell genomes inthe sample.

The practice of certain embodiments of the present invention willemploy, unless indicated specifically to the contrary, conventionalmethods in microbiology, molecular biology, biochemistry, moleculargenetics, cell biology, virology and immunology techniques that arewithin the skill of the art, and reference to several of which is madebelow for the purpose of illustration. Such techniques are explainedfully in the literature. See, e.g., Sambrook, et al., Molecular Cloning:A Laboratory Manual (3^(rd) Edition, 2001); Sambrook, et al., MolecularCloning: A Laboratory Manual (2^(nd) Edition, 1989); Maniatis et al.,Molecular Cloning: A Laboratory Manual (1982); Ausubel et al., CurrentProtocols in Molecular Biology (John Wiley and Sons, updated July 2008);Short Protocols in Molecular Biology: A Compendium of Methods fromCurrent Protocols in Molecular Biology, Greene Pub. Associates andWiley-Interscience; Glover, DNA Cloning: A Practical Approach, vol. I &II (IRL Press, Oxford Univ. Press USA, 1985); Current Protocols inImmunology (Edited by: John E. Coligan, Ada M. Kruisbeek, David H.Margulies, Ethan M. Shevach, Warren Strober 2001 John Wiley & Sons, NY,NY); Real-Time PCR: Current Technology and Applications, Edited by JulieLogan, Kirstin Edwards and Nick Saunders, 2009, Caister Academic Press,Norfolk, UK; Anand, Techniques for the Analysis of Complex Genomes,(Academic Press, New York, 1992); Guthrie and Fink, Guide to YeastGenetics and Molecular Biology (Academic Press, New York, 1991);Oligonucleotide Synthesis (N. Gait, Ed., 1984); Nucleic AcidHybridization (B. Hames & S. Higgins, Eds., 1985); Transcription andTranslation (B. Hames & S. Higgins, Eds., 1984); Animal Cell Culture (R.Freshney, Ed., 1986); Perbal, A Practical Guide to Molecular Cloning(1984); Next-Generation Genome Sequencing (Janitz, 2008 Wiley-VCH); PCRProtocols (Methods in Molecular Biology) (Park, Ed., 3^(rd) Edition,2010 Humana Press); Immobilized Cells And Enzymes (IRL Press, 1986); thetreatise, Methods In Enzymology (Academic Press, Inc., N.Y.); GeneTransfer Vectors For Mammalian Cells (J. H. Miller and M. P. Calos eds.,1987, Cold Spring Harbor Laboratory); Harlow and Lane, Antibodies, (ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1998);Immunochemical Methods In Cell And Molecular Biology (Mayer and Walker,eds., Academic Press, London, 1987); Handbook Of ExperimentalImmunology, Volumes I-IV (D. M. Weir and CC Blackwell, eds., 1986);Riott, Essential Immunology, 6th Edition, (Blackwell ScientificPublications, Oxford, 1988); Embryonic Stein Cells: Methods andProtocols (Methods in Molecular Biology) (Kurstad Turksen, Ed., 2002);Embryonic Stem Cell Protocols: Volume I: Isolation and Characterization(Methods in Molecular Biology) (Kurstad Turksen, Ed., 2006); EmbryonicStem Cell Protocols: Volume II: Differentiation Models (Methods inMolecular Biology) (Kurstad Turksen, Ed., 2006); Human Embryonic StemCell Protocols (Methods in Molecular Biology) (Kursad Turksen Ed.,2006); Mesenchymal Stem Cells: Methods and Protocols (Methods inMolecular Biology) (Darwin J. Prockop, Donald G. Phinney, and Bruce A.Bunnell Eds., 2008); Hematopoietic Stem Cell Protocols (Methods inMolecular Medicine) (Christopher A. Klug, and Craig T. Jordan Eds.,2001); Hematopoietic Stem Cell Protocols (Methods in Molecular Biology)(Kevin D. Bunting Ed., 2008) Neural Stem Cells: Methods and Protocols(Methods in Molecular Biology) (Leslie P. Weiner Ed., 2008).

Unless specific definitions are provided, the nomenclature utilized inconnection with, and the laboratory procedures and techniques of,molecular biology, analytical chemistry, synthetic organic chemistry,and medicinal and pharmaceutical chemistry described herein are thosewell known and commonly used in the art. Standard techniques may be usedfor recombinant technology, molecular biological, microbiological,chemical syntheses, chemical analyses, pharmaceutical preparation,formulation, and delivery, and treatment of patients.

Unless the context requires otherwise, throughout the presentspecification and claims, the word “comprise” and variations thereof,such as, “comprises” and “comprising” are to be construed in an open,inclusive sense, that is, as “including, but not limited to”. By“consisting of” is meant including, and typically limited to, whateverfollows the phrase “consisting of.” By “consisting essentially of” ismeant including any elements listed after the phrase, and limited toother elements that do not interfere with or contribute to the activityor action specified in the disclosure for the listed elements. Thus, thephrase “consisting essentially of” indicates that the listed elementsare required or mandatory, but that no other elements are required andmay or may not be present depending upon whether or not they affect theactivity or action of the listed elements.

In this specification and the appended claims, the singular forms “a,”“an” and “the” include plural references unless the content clearlydictates otherwise. As used herein, in particular embodiments, the terms“about” or “approximately” when preceding a numerical value indicatesthe value plus or minus a range of 5%, 6%, 7%, 8% or 9%. In otherembodiments, the terms “about” or “approximately” when preceding anumerical value indicates the value plus or minus a range of 10%, 11%,12%, 13% or 14%. In yet other embodiments, the terms “about” or“approximately” when preceding a numerical value indicates the valueplus or minus a range of 15%, 16%, 17%, 18%, 19% or 20%.

Reference throughout this specification to “one embodiment” or “anembodiment” or “an aspect” means that a particular feature, structure orcharacteristic described in connection with the embodiment is includedin at least one embodiment of the present invention. Thus, theappearances of the phrases “in one embodiment” or “in an embodiment” invarious places throughout this specification are not necessarily allreferring to the same embodiment. Furthermore, the particular features,structures, or characteristics may be combined in any suitable manner inone or more embodiments.

EXAMPLES Example 1 Design of Template Oligonucleotides for CalibratingAmplification Primer Bias Control

In this and the following Examples, standard molecular biology andbiochemistry materials and methodologies were employed, includingtechniques described in, e.g., Sambrook, et al., Molecular Cloning: ALaboratory Manual (3^(rd) Edition, 2001); Sambrook, et al., MolecularCloning: A Laboratory Manual (2^(nd) Edition, 1989); Maniatis et al.,Molecular Cloning: A Laboratory Manual (1982); Ausubel et al., CurrentProtocols in Molecular Biology (John Wiley and Sons, updated July 2008);Short Protocols in Molecular Biology: A Compendium of Methods fromCurrent Protocols in Molecular Biology, Greene Pub. Associates andWiley-Interscience; Glover, DNA Cloning: A Practical Approach, vol. I &II (IRL Press, Oxford Univ. Press USA, 1985); Anand, Techniques for theAnalysis of Complex Genomes, (Academic Press, New York, 1992);Oligonucleotide Synthesis (N. Gait, Ed., 1984); Nucleic AcidHybridization (B. Hames & S. Higgins, Eds., 1985); Transcription andTranslation (B. Hames & S. Higgins, Eds., 1984); Perbal, A PracticalGuide to Molecular Cloning (1984); Next-Generation Genome Sequencing(Janitz, 2008 Wiley-VCH); PCR Protocols (Methods in Molecular Biology)(Park, Ed., 3^(rd) Edition, 2010 Humana Press).

A set of double-stranded DNA (dsDNA) template oligonucleotides wasdesigned as a calibration standard for use as a control template thatsimulated all possible V/J combinations at a specified adaptive immunereceptor (TCR or BCR) locus. For each human TCR and BCR locus, a listwas compiled of the known genomic V segment sequences 5′ of the RSS, anda list of the known genomic J segments 3′ of the RSS. The coding strandsequences of the dsDNA template are presented here for ease ofinterpretation, according to the convention by which the 5′-to-3′orientation is read left-to-right.

A schematic representation of the general structure of the templateoligonucleotides is shown in FIG. 1. For use in cross-validation of eachunique template oligonucleotide's identity in multiple contexts, adifferent 16 bp barcode oligonucleotide (B) was incorporated into eachtemplate that uniquely identified the V segment polynucleotide of thetemplate with the first 8 bp of the barcode, and the J segment with thesecond 8 bp of the barcode. Copies of this barcode were incorporatedthrice: (B3) between the external adapter (U2) and the J segmentsequence (J) so that a short single-end read with standard Illumina orIon primers can reveal the identity of the unique combination of V and Jsequences in each template oligonucleotide, (B2) between the V and Jsegments so that a standard sequencing strategy (e.g., Illumina GA-2 orHiSeg™ or MiSEQ®) will capture the unique combination of V and Jsequences in each template oligonucleotide, and (B3) between the Vsegment and the other external adapter (U1), so that a short paired-endread can confirm the identity of the unique combination of V and Jsequences in each template oligonucleotide if so desired.

As shown in FIG. 1, the template oligonucleotide sequences started withan adapter sequence (U1) that was capable of incorporating sequencingplatform-specific short oligonucleotide sequences at the ends of themolecule. In this example the Illumina Nextera™ adaptors were used, butit should be noted that essentially any pair of robust PCR primers wouldwork equally well. As an exemplary adapter, the oligonucleotide sequenceGCCTTGCCAGCCCGCTCAG [SEQ ID NO:1746] was attached at the V segment endof U1 (FIG. 1), in order to maintain compatibility with the Nextera™Illumina Adaptor (Illumina, Inc., San Diego, Calif.)(CAAGCAGAAGACGGCATACGAGATCGGTCTGCCTTGCCAGCCCGCTCAG) [SEQ ID NO:1747] toadd on the standard Illumina oligonucleotide, which was compatible witheither single or paired end Illumina sequencing flowcclls.

Immediately downstream from (3′ to) U1 was the first copy (B1) of thebarcode oligonucleotide ACACACGTGACACTCT [SEQ ID NO:1748]. Next, a fixedlength of V segment sequence was incorporated into the templateoligonucleotide, with all templates in the template set ending a givennumber of bases before the natural RSS, in order to mimic a natural TCRor BCR gene rearrangement having a fixed number of bases deleted at theV segment. In this example zero bases were initially deleted before theRSS. To maximize the recognizability of these sequences, all V segmentpolynucleotide sequences were then trimmed to remove partial codonsadjacent to the RSS, so that the residual V segment sequences were inframe with the start codon. Diverse V segment sequences were those shownin the exemplary template oligonucleotide sets presented in the SequenceListing (e.g., a set of TCRB V segments within the formula (I) sequencesof the TCRB template oligonucleotide set in SEQ ID NOS:1-871; a distinctset of TCRB V segments within the formula (I) sequences of the TCRBtemplate oligonucleotide set in SEQ ID NOS:872-1560; a set of TCRG Vsegments within the formula (I) sequences of the TCRG templateoligonucleotide set in SEQ ID NOS:1561-1630); a single exemplary Vpolynucleotide was as follows:

TCTTATTTTCATAGGCTCCATGGATACTGGAATTACCCAGACACCAAAATACCTGGTCACAGCAATGGGGAGTAAAAGGACAATGAAACGTGAGCATCTGGGACATGATTCTATGTATTGGTACAGACAGAAAGCTAAGAAATCCCTGGAGTTCATGTTTTACTACAACTGTAAGGAATTCATTGAAAACAAGACTGTGCCAAATCACTTCACACCTGAATGCCCTGACAGCTCTCGCTTATACCTTCATGTGGTCGCACTGCAGCAAGAAGACTCAGCTGCGTATCTCTGCACCAGCAG [SEQ ID NO:1749].

The stop codon TGA was incorporated in-frame at the 3′ end of the Vpolynucleotide sequence in each template oligonucleotide, to ensure thatthe template oligonucleotide sequences would not be considered relevantin the event they contaminated a biological sample. Downstream from thestop codon, between the V segment and J segment where the NDN wouldnormally be, the second copy of the V/J identifier barcode sequence B2(SEQ ID NO:1748) was inserted. Next the Sal1 restriction enzymerecognition site (R) sequence GTCGAC was incorporated; this sequence wasselected on the basis of being a sequence that was not naturally presentin any of the TCRB V or J segment genomic sequences, conferring theability to specifically destroy the synthetic template if desired, orfor use as an informatic marker to identify the synthetic sequences. TheB3 site, in this version of the template is empty.

The J polynucleotide (J) was incorporated as a fixed length of sequencefrom a J gene segment, measured from a fixed number of bases after thenatural RSS to mimic a natural rearrangement, and in the present exampleextending into the J-C intron. In this example zero bases were deletedbases from the J segment, but in other template oligonucleotide designsa deletion of 5 bp was used to make room for the VJ barcode (B2) at theV−J junction while maintaining an overall J segment length in thenatural range. An exemplary J polynucleotide was

ACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAGGTAAGACATTTTTCAGGTTCTTTTGCAGATCCGTCACAGGGAAAAGTGGGTCCACAG [SEQ ID NO:1750].

Downstream from the J segment polynucleotide was the third copy (B4) ofthe V/J barcode identifier oligonucleotide (SEQ ID NO:1748). Theexemplary template oligonucleotide sequence the sequence ended with asecond adapter sequence (U2) that was capable of incorporatingplatform-specific sequences at the ends of the molecule. As noted above,a Nextera™-compatible adaptor (CTGATGGCGCGAGGGAGGC) [SEQ ID NO:1751] wasused on the J segment end of U2, for use with the Nextera™ IlluminaAdaptor (AATGATACGGCGACCACCGAGATCTACACGCCTCCCTCGCGCCATCAG) [SEQ IDNO:1752] to permit adding on the standard Illumina sequencingoligonucleotide, which is compatible with either single or paired endflowcells.

Exemplary TCRB and TCRG template oligonucleotide sets according to thepresent disclosure were prepared and had the nucleotide sequences setforth in SEQ ID NOS:1-1630. The sets of template oligonucleotides havingsequences set forth in SEQ ID NOS:1-871 and 1561-1630 were customsynthesized, based on the sequence design information disclosed herein,by Integrated DNA Technologies, Inc. (Coralville, Iowa) using gBlocks™Gene Fragments chemistry. The set of template oligonucleotides havingsequences set forth in SEQ TD NOS:872-1560 was generated by a PCR tilingapproach described in Example 2.

TCRB Template Oligonucleotides (SEQ ID NOS:1-871). A set of 871 templateoligonucleotides of general formula (I) (in which B3 is nothing) wasdesigned using human TCRB V and J polynucleotide sequences:5′-U1-B1-V-B2-R-(B3)-J-B4-U2-3′  (I).

Each template oligonucleotide consisted of a 495 base pair DNA molecule.Sense strand sequences are presented as SEQ TD NOS:1-871.

A schematic diagram depicting the design of this template set is shownin FIG. 1. By convention, the diagram depicts the oligonucleotide designin the 5′-to 3′ (left-to-right) direction. “V segment” represents anadaptive immune receptor variable (V) region encoding gene sequence, orthe complement thereof “J segment” represents an adaptive immunereceptor joining (J) region encoding gene sequence, or the complementthereof. U1 and U2 represent, respectively, first and second universaladaptor oligonucleotide sequences, which may optionally furthercomprise, respectively, first and second sequencing platform-specificoligonucleotide sequences linked to and positioned 5′ to the first andsecond universal adaptor oligonucleotide sequences. B1, B2 and B4represent oligonucleotide barcode sequences that each comprise anoligonucleotide barcode sequence comprising a unique oligonucleotidesequence that uniquely identifies, as a paired combination, (i) a uniqueV segment sequence, and (ii) a unique J segment sequence; in thisExample, B3 was nothing.

S represents an optional stop codon that may be in-frame or out of frameat the 3′ end of V. R represents an optional restriction enzymerecognition site. In SEQ ID NOS:1-871 the U1 and U2 adapters includedthe 19-mers as described above (SEQ ID NOS:1746 and 1751, respectively)and all (V+J)-identifying barcode (B) sequences (B1, B2, B4) were 16nucleotides in length; the stop codon TGA and the Sal1 restrictionenzyme recognition site (GTCGAC) were included.

TCRB Template Oligonucleotides (SEQ ID NOS:872-1560). A second set of689 template oligonucleotides was designed in which, according togeneral formula (I), V and J comprised, respectively, human TCRB V and Jpolynucleotide sequences, U1 and U2 independently comprised distinctrestriction enzyme recognition sites (R1 and R3), and B1, B3, and B4were independently nothing, to arrive at general formula (II):R1-V-B2-R2-J-R3  (II)

wherein B2 was an 8-nucleotide barcode identifier (e.g., a barcodesequence as set forth in Table 7); R1, R2 and R3 were, respectively, therestriction enzyme recognition sites EcoR1 (GAATTC), Sal1 (GTCGAC) andSph1 (GCATGC); and V and J were, respectively, V region and J regionpolynucleotides as described herein. Each template oligonucleotideconsisted of a 239 base pair DNA molecule. Sense strand sequences arepresented as SEQ ID NOS:872-1560.

TCRG Template Oligonucleotides (SEQ ID NOS:1561-1630). A third set of 70template oligonucleotides of general formula (I) was designed usinghuman TCRG V and J polynucleotide sequences. Each templateoligonucleotide consisted of a 495 base pair DNA molecule. Sense strandsequences are presented as SEQ ID NOS:1561-1630. Details for the70-oligonucleotide set of TCRG templates (SEQ ID NOS:1561-1630) arerepresentative and were as follows:

Based on previously determined genomic sequences the human TCRG locuswas shown to contain 14 Vγ segments that each had a RSS sequence andwere therefore regarded as rearrangement-competent. These 14 Vγ segmentsincluded six gene segments known to be expressed, three V segments thatwere classified as having open reading frames, and five V pseudogenes.The Vγ gene segments were linked to five Jγ gene segments. In order toinclude all possible V+J gene combinations for the 14 V and 5 Jsegments, 70 (5×14) templates were designed that represented allpossible VJ combinations. Each template conformed to the general formula(I) (5′-U1-B1-V-B2-R-(B3)-J-B4-U2-3′)(FIG. 1) and thus included ninesections, a 19 base pair (bp) universal adapter (U1), a 16 bp nucleotidetag uniquely identifying each paired combination of V gene and J genesegments (B1), 300 bp of V gene specific sequence (V), a 3 bp stop codon(S), another copy of the 16 bp nucleotide tag (B2), a 6 bp junction tagshared by all molecules (R), nothing for B3, 100 bp of J gene specificsequence (J), a third copy of the 16 bp nucleotide tag (B4), and a 19 bpuniversal adapter sequence (U2).

Each of the 70 templates (SEQ ID NOS:1561-1630) was amplifiedindividually using oligonucleotide primers (Table 4; SEQ IDNOS:1732-1745) designed to anneal to the universal adapter sequences(U1, U2).

TABLE 4 TCRG Amplification Primers 5′ SEQ ID Primer Name AdapterSequence NO: TCRGV01_dev10 pGEXf GGAGGGGAAGGCCCCACAGTGTCTTC 1732TCRGV02/3/4/5/8_dev10 pGEXf GGAGGGGAAGGCCCCACAGCGTCTTC 1733TCRGV05P_dev10 pGEXf GGAGGGGAAGACCCCACAGCATCTTC 1734 TCRGV06_dev10 pGEXfGGAGGGGAAGGCCCCACAGCATCTTC 1735 TCRGV07_dev10 pGEXfGGCGGGGAAGGCCCCACAGCATCTTC 1736 TCRGV09_dev10 pGEXfTGAAGTCATACAGTTCCTGGTGTCCAT 1737 TCRGV10_dev10 pGEXfCCAAATCAGGCTTTGGAGCACCTGATCT 1738 TCRGV11_dev10 pGEXfCAAAGGCTTAGAATATTTATTACATGT 1739 TCRGVA_dev10 pGEXfCCAGGTCCCTGAGGCACTCCACCAGCT 1740 TCRGVB_dev10 pGEXfCTGAATCTAAATTATGAGCCATCTGACA 1741 TCRGJP1_dev10 pGEXrGTGAAGTTACTATGAGCTTAGTCCCTTC 1742 AGCAAA TCRGJP2_dev10 pGEXrCGAAGTTACTATGAGCCTAGTCCCTTTT 1743 GCAAA TCRGJ1/2_dev10 pGEXrTGACAACAAGTGTTGTTCCACTGCCAAA 1744 TCRGJP_dev10 pGEXrCTGTAATGATAAGCTTTGTTCCGGGACC 1745 AAA

The resulting concentration of each amplified template oligonucleotideproduct was quantified using a LabChip GX™ capillary electrophoresissystem (Caliper Life Sciences, Inc., Hopkinton, Mass.) according to themanufacturer's instructions. The frequencies of occurrence for each ofthe 70 possible V−J combinations, as determined by sequencing barcodesB1, are shown in Table 5. The 70 amplified template oligonucleotidepreparations were normalized to a standard concentration and thenpooled.

To verify that all 70 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina HiSeq™ sequencing platform according to the manufacturer'srecommendations. Briefly, to incorporate platform-specificoligonucleotide sequences into the pooled template oligonucleotides,tailed primers were designed that annealed to the universal primingsites (U1, U2) and that had Illumina Nextera™ adapter sequence tails asthe 5′ ends. A seven-cycle PCR reaction was then performed to anneal theIllumina adapters to the template oligonucleotides. The PCR reactionproduct mixture was then purified using Agencourt® AMPure® XP beads(Beckman Coulter, Inc., Fullerton, Calif.) under the conditionsrecommended by the manufacturer. The first 60 bp of the PCR reactionproducts were sequenced using an Illumina HiSEQ™ sequencer (Illumina,Inc., San Diego, Calif.) and analyzed by assessing the frequency of each16 bp molecular barcode tag (B1).

A substantially equimolar preparation for the set of 70 distincttemplate oligonucleotides was calculated to contain approximately 1.4%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (0.14-14%) for all species was desired. Thequantitative sequencing revealed that the 70 species of adapter-modifiedtemplate oligonucleotides within the initial pool were not evenlyrepresented.

Accordingly, adjustment of the concentrations of individual templateoligonucleotides and reiteration of the quantitative sequencing stepsare conducted until each molecule is present within the thresholdtolerance concentration (0.14-14%).

TABLE 5 Relative Representation (number of occurrences of indicated V-Jcombination) of amplification products of each TCRG VJ pair (14 V × 5 J)in pre-amplification Template Pool Count of Jseg B Labels TCRGJ TCRGJ2TCRGJP TCRGJP1 TCRGJP2 #N/A Grand Total TCRGV01 17 308 1315 741 822 443247 TCRGV02 630 781 2394 2009 122 65 6001 TCRGV03 250 166 2119 157 110551 3848 TCRGV04 777 37 2031 1490 1443 76 5854 TCRGV05 323 93 2571 716150 63 3916 TCRGV05P 294 1161 2946 1552 530 111 6594 TCRGV06 164 12801809 401 23 40 3717 TCRGV07 16 234 1849 1697 93 78 3967 TCRGV08 2523 653944 170 134 57 4481 TCRGV09 55 1004 2057 124 228 42 3510 TCRGV10 351 690814 384 466 36 2741 TCRGV11 505 648 639 330 181 39 2342 TCRGVA 199 475112 272 437 12 1507 TCRGVB 210 20 423 874 917 24 2468 #N/A 77 118 309150 106 531 1291 Grand Total 6391 7668 22332 11067 6757 1269 55484

Example 2 Detection of TCRB V Gene Amplification Bias

This example describes how a set of 689 human TCRB templateoligonucleotides of general formula (I) was assembled by tiling togetherfour single stranded oligonucleotides of 50-90 nucleotides each togenerate a template set containing hybridization targets for allpossible V−J combinations in a set of oligonucleotide primers that wascapable of amplifying human TCRB sequences. The set of templateoligonucleotides was then used to characterize the relativeamplification efficiencies of a set of TCRB V and J amplificationprimers.

A set of TCRB 689 template oligonucleotides containing polynucleotidesequences representing all possible productively rearranged V and Jcombinations for human TCRB chains was synthesized by “tiling” togetherfour single-stranded DNA primers in a standard PCR reaction. Briefly,two 90 bp fragments (one in “forward” orientation and the other in“reverse”) were designed for each TCRB V gene segment, one 90 bpfragment (in “reverse” orientation) was designed for each TCRB J genesegment, and a 50 bp (forward) linker molecule was designed to linktogether the V and J gene fragments. In total, 52 V forward and 52 Vreverse, 13 J reverse, and 689 linker molecules were designed. The two90 bp fragments (one forward, one reverse) that corresponded to each ofthe V gene segments had 39 bp of complementary overlapping sequence. Oneend of each V reverse fragment had 25 bp of complementary sequence whichoverlapped with the 50 bp linker molecule. The remaining 25 bp in eachof the linker molecules was a sequence that complementarily overlappedwith one end of the J molecule. The molecules were designed so that thecomplementary sequences would anneal to one another and form doublestranded DNA to which Taq polymerase could bind and enzymatically extendthe molecule.

Each PCR reaction to assemble the tiled molecules used QIAGEN MultiplexPCR master mix (QIAGEN part number 206145, Qiagen, Valencia, Calif.),10% Q-solution (QIAGEN), and the four single-stranded oligonucleotidesequences (two TCRB V, a TCRB J and a linker, as described above). Thetwo external molecules (one V forward and one J reverse) were added at afinal concentration of 1 μM each while the two internal molecules, (oneV reverse and the forward linker), were each added at a finalconcentration of 0.01 μM. The thermocycler conditions were: 95° C. for15 minutes, followed by 35 cycles of 94° C. for 30 seconds, 59° C. for30 seconds, and 72° C. for 1 minute, followed by 1 cycle at 72° C. for10 minutes. After synthesis, the molecules were quantified by theLabChip GX™ capillary electrophoresis system (Caliper Life Sciences,Inc., Hopkinton, Mass.) according to the manufacturer's instructions andthe concentration (in ng/μl) of each resulting band was calculated usingCaliper LabChip GX software.

The nucleotide sequences for the resulting set of 689 TCRB templateoligonucleotides are set forth in SEQ ID NOS:872-1560. In SEQ IDNOS:872-1560, each distinct V region sequence was identified by a uniquebarcode sequence of eight nucleotides, as shown in Table 7. All 689templates were normalized to a standard concentration of 25 ng/μl, andthen pooled. The resulting pool was used for the TCRB assays describedherein to detect biased (non-uniform) utilization of TCRB amplificationprimers during amplification of the 689-template oligonucleotide set(SEQ ID NOS:872-1560).

Each of the 689 templates was present in the template oligonucleotidepool at experimentally as close as possible to equal molarconcentration, and the pool was used as template for the TCRBamplification PCR reaction using an equimolar mixture of 52 TCRB Vregion primers that included an Illumina adapter-compatible sequence(SEQ ID NOS:1753-1804, Table 6) and an equimolar mix of 13 TCRB J regionprimers (SEQ ID NOS:1631-1643, Table 1). The members of the pool of 689templates were amplified using an equimolar pool of the 52 TCRB JβR(forward) primers (the “JF pool”) and an equimolar pool of the 13 TCRBJβR (reverse) primers (the “JR pool”) as shown in Table 1 (SEQ IDNOS:1.631-1695). Polymerase chain reactions (PCR) (50 μL each) were setup at 1.0 μM VF pool (22 nM for each unique TCRB VβF primer), 1.0 μM JRpool (77 nM for each unique TCRB JβR primer), 1 μM QIAGEN Multiplex PCRmaster mix ((NAG-EN part number 206145, Qiagen Corp., Valencia, Calif.),10% Q-solution (QIAGEN), and 16 ng/μL genomic DNA (gDNA). The followingthermal cycling conditions were used in a C100 thermal cycler (Bio-RadLaboratories, Hercules, Calif., USA): one cycle at 95° C. for 15minutes, 25 to 40 cycles at 94° C. for 30 seconds, 59° C. for 30seconds, and 72° C. for one minute, followed by one cycle at 72° C. for10 minutes. To sample millions of rearranged TCRβ CDR3 loci, 12 to 20wells of PCR were performed for each library. As noted above, the V andJ primers included a tail that corresponded to, and was compatible with,Illumina adapters for sequencing.

Amplification products were quantitatively sequenced on an IlluminaHiSeg™ sequencer. A 60-base pair region of each product molecule wassequenced using standard J sequencing primers (Table 3) starting fromthe J molecules. The frequencies of occurrence of each TCRB sequence inthe reaction products are shown in FIG. 2, from which it was apparentthat not all TCRB sequences had been amplified to a comparable degree.

TABLE 6 TCRB Amplification Primers Adjusted Relative Primer MolarPrimer Name Primer Sequence Ratio SEQ ID NO: TRB2V10-1CAA GCA GAA GAC GGC ATA CGA 0.77 1753 GCT CTT CCG ATC TAA CAA AGGAGA AGT CTC AGA TGG CTA CAG TRB2V10-2 CAA GCA GAA GAC GGC ATA CGA 1.571754 GCT CTT CCG ATC TGA TAA AGG AGA AGT CCC CGA TGG CTA TGT TRB2V10-3CAA GCA GAA GAC GGC ATA CGA 2.76 1755 GCT CTT CCG ATC TGA CAA AGGAGA AGT CTC AGA TGG CTA TAG TRB2V11-123 CAA GCA GAA GAC GGC ATA CGA 1.881756 GCT CTT CCG ATC TCT AAG GAT CGA TTT TCT GCA GAG AGG CTC TRB2V12-1CAA GCA GAA GAC GGC ATA CGA 1 1757 GCT CTT CCG ATC TTT GAT TCTCAG CAC AGA TGC CTG ATG T TRB2V12-2 CAA GCA GAA GAC GGC ATA CGA 1 1758GCT CTT CCG ATC TGC GAT TCT CAG CTG AGA GGC CTG ATG G TRB2V12-3/4CAA GCA GAA GAC GGC ATA CGA 3.24 1759 GCT CTT CCG ATC TTC GAT TCTCAG CTA AGA TGC CTA ATG C TRB2V12-5 CAA GCA GAA GAC GGC ATA CGA 1.821760 GCT CTT CCG ATC TTT CTC AGC AGA GAT GCC TGA TGC AAC TTT A TRB2V13CAA GCA GAA GAC GGC ATA CGA 2.14 1761 GCT CTT CCG ATC TCT GAT CGATTC TCA GCT CAA CAG TTC AGT TRB2V14 CAA GCA GAA GAC GGC ATA CGA 1.651762 GCT CTT CCG ATC TTC TTA GCT GAA AGG ACT GGA GGG ACG TAT TRB2V15CAA GCA GAA GAC GGC ATA CGA 3.77 1763 GCT CTT CCG ATC TGC CGA ACACTT CTT TCT GCT TTC TTG AC TRB2V16 CAA GCA GAA GAC GGC ATA CGA 1.40 1764GCT CTT CCG ATC TTT CAG CTA AGT GCC TCC CAA ATT CAC CCT TRB2V17CAA GCA GAA GAC GGC ATA CGA 2.87 1765 GCT CTT CCG ATC TAT TCA CAGCTG AAA GAC CTA ACG GAA CGT TRB2V18 CAA GCA GAA GAC GGC ATA CGA 0.801766 GCT CTT CCG ATC TAT TTT CTG CTG AAT TTC CCA AAG AGG GCC TRB2V19CAA GCA GAA GAC GGC ATA CGA 0.84 1767 GCT CTT CCG ATC TTA TAG CTGAAG GGT ACA GCG TCT CTC GGG TRB2V2 CAA GCA GAA GAC GGC ATA CGA 1.02 1768GCT CTT CCG ATC TTT CGA TGA TCA ATT CTC AGT TGA AAG GCC TRB2V20-1CAA GCA GAA GAC GGC ATA CGA 1.66 1769 GCT CTT CCG ATC TAT GCA AGCCTG ACC TTG TCC ACT CTG ACA TRB2V23-1 CAA GCA GAA GAC GGC ATA CGA 1 1770GCT CTT CCG ATC TGA TTC TCA TCT CAA TGC CCC AAG AAC GC TRB2V24-1CAA GCA GAA GAC GGC ATA CGA 4.01 1771 GCT CTT CCG ATC TAT CTC TGATGG ATA CAG TGT CTC TCG ACA TRB2V25-1 CAA GCA GAA GAC GGC ATA CGA 1.291772 GCT CTT CCG ATC TTT TCC TCT GAG TCA ACA GTC TCC AGA ATA TRB2V26CAA GCA GAA GAC GGC ATA CGA 1 1773 GCT CTT CCG ATC TCT CTG AGAGGT ATC ATG TTT CTT GAA ATA TRB2V27 CAA GCA GAA GAC GGC ATA CGA 4.221774 GCT CTT CCG ATC TTC CTG AAG GGT ACA AAG TCT CTC GAA AAG TRB2V28CAA GCA GAA GAC GGC ATA CGA 2.37 1775 GCT CTT CCG ATC TTC CTG AGGGGT ACA GTG TCT CTA GAG AGA TRB2V29-1 CAA GCA GAA GAC GGC ATA CGA 1.501776 GCT CTT CCG ATC TCA TCA GCC GCC CAA ACC TAA CAT TCT CAA TRB2V2PCAA GCA GAA GAC GGC ATA CGA 1 1777 GCT CTT CCG ATC TCC TGA ATGCCC TGA CAG CTC TCG CTT ATA TRB2V3-1 CAA GCA GAA GAC GGC ATA CGA 3.351778 GCT CTT CCG ATC TCC TAA ATC TCC AGA CAA AGC TCA CTT AAA TRB2V3-2CAA GCA GAA GAC GGC ATA CGA 1 1779 GCT CTT CCG ATC TCT CAC CTGACT CTC CAG ACA AAG CTC AT TRB2V30 CAA GCA GAA GAC GGC ATA CGA 1.48 1780GCT CTT CCG ATC TGA CCC CAG GAC CGG CAG TTC ATC CTG AGT TRB2V4-1CAA GCA GAA GAC GGC ATA CGA 3.32 1781 GCT CTT CCG ATC TCT GAA TGCCCC AAC AGC TCT CTC TTA AAC TRB2V4-2/3 CAA GCA GAA GAC GGC ATA CGA 3.111782 GCT CTT CCG ATC TCT GAA TGC CCC AAC AGC TCT CAC TTA TTC TRB2V5-1CAA GCA GAA GAC GGC ATA CGA 1.27 1783 GCT CTT CCG ATC TTG GTC GATTCT CAG GGC GCC AGT TCT CTA TRB2V5-3 CAA GCA GAA GAC GGC ATA CGA 1.751784 GCT CTT CCG ATC TTA ATC GAT TCT CAG GGC GCC AGT TCC ATG TRB2V5-4CAA GCA GAA GAC GGC ATA CGA 1.58 1785 GCT CTT CCG ATC TTC CTA GATTCT CAG GTC TCC AGT TCC CTA TRB2V5-5 CAA GCA GAA GAC GGC ATA CGA 0.991786 GCT CTT CCG ATC TAA GAG GAA ACT TCC CTG ATC GAT TCT CAG C TRB2V5-6CAA GCA GAA GAC GGC ATA CGA 0.69 1787 GCT CTT CCG ATC TGG CAA CTTCCC TGA TCG ATT CTC AGG TCA TRB2V5-8 CAA GCA GAA GAC GGC ATA CGA 3.301788 GCT CTT CCG ATC TGG AAA CTT CCC TCC TAG ATT TTC AGG TCG TRB2V6-1CAA GCA GAA GAC GGC ATA CGA 1.74 1789 GCT CTT CCG ATC TGT CCC CAATGG CTA CAA TGT CTC CAG ATT TRB2V6-2/3 CAA GCA GAA GAC GGC ATA CGA 1.591790 GCT CTT CCG ATC TGC CAA AGG AGA GGT CCC TGA TGG CTA CAA TRB2V6-4CAA GCA GAA GAC GGC ATA CGA 1.48 1791 GCT CTT CCG ATC TGT CCC TGATGG TTA TAG TGT CTC CAG AGC TRB2V6-5 CAA GCA GAA GAC GGC ATA CGA 0.451792 GCT CTT CCG ATC TAA GGA GAA GTC CCC AAT GGC TAC AAT GTC TRB2V6-6CAA GCA GAA GAC GGC ATA CGA 0.41 1793 GCT CTT CCG ATC TGA CAA AGGAGA AGT CCC GAA TGG CTA CAA C TRB2V6-7 CAA GCA GAA GAC GGC ATA CGA 2.231794 GCT CTT CCG ATC TGT TCC CAA TGG CTA CAA TGT CTC CAG ATC TRB2V6-8CAA GCA GAA GAC GGC ATA CGA 1.18 1795 GCT CTT CCG ATC TCT CTA GATTAA ACA CAG AGG ATT TCC CAC TRB2V6-9 CAA GCA GAA GAC GGC ATA CGA 0.961796 GCT CTT CCG ATC TAA GGA GAA GTC CCC GAT GGC TAC AAT GTA TRB2V7-1CAA GCA GAA GAC GGC ATA CGA 0.85 1797 GCT CTT CCG ATC TTC CCC GTGATC GGT TCT CTG CAC AGA GGT TRB2V7-2 CAA GCA GAA GAC GGC ATA CGA 0.641798 GCT CTT CCG ATC TAG TGA TCG CTT CTC TGC AGA GAG GAC TGG TRB2V7-3CAA GCA GAA GAC GGC ATA CGA 0.84 1799 GCT CTT CCG ATC TGG CTG CCCAAC GAT CGG TTC TTT GCA GT TRB2V7-4 CAA GCA GAA GAC GGC ATA CGA 0.481800 GCT CTT CCG ATC TGG CGG CCC AGT GGT CGG TTC TCT GCA GAG TRB2V7-6/7CAA GCA GAA GAC GGC ATA CGA 1.01 1801 GCT CTT CCG ATC TAT GAT CGGTTC TCT GCA GAG AGG CCT GAG G TRB2V7-8 CAA GCA GAA GAC GGC ATA CGA 1.571802 GCT CTT CCG ATC TGC TGC CCA GTG ATC GCT TCT TTG CAG AAA TRB2V7-9CAA GCA GAA GAC GGC ATA CGA 0.49 1803 GCT CTT CCG ATC TGG TTC TCTGCA GAG AGG CCT AAG GGA TCT TRB2V9 CAA GCA GAA GAC GGC ATA CGA 3.46 1804GCT CTT CCG ATC TGT TCC CTG ACT TGC ACT CTG AAC TAA AC

TABLE 7 Barcode sequences used to identify TCRB VRegions in SEQ ID NOS: 872-1560 TCRBV region name Nucleotideof 8 bp barcode Sequence SEQ ID NO TCRBV2_8bpBC CAAGGTCA SEQ ID NO: 6375TCRBV3-1_8bpBC TACGTACG SEQ ID NO: 6376 TCRBV4-1_8bpBC TACGCGTTSEQ ID NO: 6377 TCRBV4-2_8bpBC CTCAGTGA SEQ ID NO: 6378 TCRBV4-3_8bpBCGTGTCTAC SEQ ID NO: 6379 TCRBV5-1_8bpBC AGTACCGA SEQ ID NO: 6380TCRBV5-3_8bpBC TTGCCTCA SEQ ID NO: 6381 TCRBV5-4_8bpBC TCGTTAGCSEQ ID NO: 6382 TCRBV5-5_8bpBC TGGACATG SEQ ID NO: 6383 TCRBV5-6_8bpBCAGGTTGCT SEQ ID NO: 6384 TCRBV5-7_8bpBC GTACAGTG SEQ ID NO: 6385TCRBV5-8_8bpBC ATCCATGG SEQ ID NO: 6386 TCRBV6-1_8bpBC TGATGCGASEQ ID NO: 6387 TCRBV6-2_8bpBC GTAGCAGT SEQ ID NO: 6388 TCRBV6-3_8bpBCGGATCATC SEQ ID NO: 6389 TCRBV6-4_8bpBC GTGAACGT SEQ ID NO: 6390TCRBV6-5_8bpBC TGTCATCG SEQ ID NO: 6391 TCRBV6-6_8bpBC AGGCTTGASEQ ID NO: 6392 TCRBV6-7_8bpBC ACACACGT SEQ ID NO: 6393 TCRBV6-8_8bpBCTCCACAGT SEQ ID NO: 6394 TCRBV6-9_8bpBC CAGTCTGT SEQ ID NO: 6395TCRBV7-1_8bpBC TCCATGTG SEQ ID NO: 6396 TCRBV7-2_8bpBC TCACTGCASEQ ID NO: 6397 TCRBV7-3_8bpBC CAAGTCAC SEQ ID NO: 6398 TCRBV7-4_8bpBCTAGACGGA SEQ ID NO: 6399 TCRBV7-6_8bpBC GAGCGATA SEQ ID NO: 6400TCRBV7-7_8bpBC CTCGAGAA SEQ ID NO: 6401 TCRBV7-8_8bpBC ATGACACCSEQ ID NO: 6402 TCRBV7-9_8bpBC CTTCACGA SEQ ID NO: 6403 TCRBV9_8bpBCCGTAGAGT SEQ ID NO: 6404 TCRBV10-1_8bpBC TCGTCGAT SEQ ID NO: 6405TCRBV10-2_8bpBC AGCTAGTG SEQ ID NO: 6406 TCRBV10-3_8bpBC TGAGACCTSEQ ID NO: 6407 TCRBV11-1_8bpBC GATGGCTT SEQ ID NO: 6408 TCRBV11-2_8bpBCGCATCTGA SEQ ID NO: 6409 TCRBV11-3_8bpBC GACACTCT SEQ ID NO: 6410TCRBV12-3_8bpBC TGCTACAC SEQ ID NO: 6411 TCRBV12-4_8bpBC TCAGCTTGSEQ ID NO: 6412 TCRBV12-5_8bpBC TTCGGAAC SEQ ID NO: 6413 TCRBV13_8bpBCGCAATTCG SEQ ID NO: 6414 TCRBV14_8bpBC CAAGAGGT SEQ ID NO: 6415TCRBV15_8bpBC GAATGGAC SEQ ID NO: 6416 TCRBV16_8bpBC AACTGCCASEQ ID NO: 6417 TCRBV17p_8bpBC CCTAGTAG SEQ ID NO: 6418 TCRBV18_8bpBCCTGACGTT SEQ ID NO: 6419 TCRBV19_8bpBC TGCAGACA SEQ ID NO: 6420TCRBV20-1_8bpBC AGTTGACC SEQ ID NO: 6421 TCRBV24-1_8bpBC GTCTCCTASEQ ID NO: 6422 TCRBV25-1_8bpBC CTGCAATC SEQ ID NO: 6423 TCRBV27-1_8bpBCTGAGCGAA SEQ ID NO: 6424 TCRBV28_8bpBC TTGGACTG SEQ ID NO: 6425TCRBV29-1_8bpBC AGCAATCC SEQ ID NO: 6426 TCRBV30_8bpBC CGAACTACSEQ ID NO: 6427

Using the data that were obtained to generate FIG. 2, as describedabove, the cross-amplification capability (ability to amplify a V genesegment other than the one for which the primer was specificallydesigned on the basis of annealing sequence complementarity) wasassessed for each amplification primer that had been designed to annealto a specific V gene segment. 52 independent amplification primer poolswere prepared, where each primer pool had 51 of the 52 TCRB V regionprimers of Table 6 pooled at equimolar concentrations, and the 52^(nd)TCRB V region primer present in the pool at twice the molarconcentration of the other 51 primers. A separate amplification primerpool was prepared so that there was one pool for each of the 52 Vprimers in which a single primer was present at twice the concentrationof the other primers, resulting in 52 unique primer pools. 52 separateamplification reactions were then set up, one for each of the uniqueamplification primer pools, with each reaction using the set of 689template oligonucleotides (SEQ ID NOS:872-1560) described above.Template oligonucleotides were present at equimolar concentrationrelative to one another. Amplification and sequencing were conductedusing the conditions described above. The results are shown in FIG. 3.

In FIG. 3, black squares indicated no change in the degree ofamplification with the respective indicated TCRB V region-specificprimer present at twice the concentration relative to equimolarconcentrations of all other primers; white squares indicated a 10-foldincrease in amplification; grey squares indicated intermediate degrees(on a greyscale gradient) of amplification between zero and 10-fold. Thediagonal line indicated that doubling the molar concentration for agiven primer resulted in about a 10-fold increase in the amplificationof the respective template oligonucleotide having the specific annealingtarget sequence, in the case of most of the TCRB V regions primers thatwere tested. The off-diagonal white squares indicated non-correspondingtemplates to which certain primers were able to anneal and amplify.

Where one or more primers exhibited amplification potential that wassignificantly greater or lower than an acceptable range of amplificationpotential (e.g., a designated uniform amplification potential range),further adjustment of the concentrations of individual primeroligonucleotides and reiteration of the template amplification andquantitative sequencing steps were conducted, until each species ofproduct molecule was present within a desired range that was indicativeof correction of the non-uniform amplification potential among theprimers within an amplification primer set.

Accordingly, primer concentrations were adjusted as indicated in Table6, in order to determine whether biased amplification results that wereapparent in FIGS. 2 and 3 could be reduced in severity by increasing ordecreasing the relative presence of, respectively, highly efficient orpoorly efficient amplification primers. For multiplexed PCR using anadjusted primer set, the V gene primer sequences remained the same(sequence reported in table 6), however the relative concentration ofeach primer was either increased, if the primer under-amplified itstemplate (FIG. 3), or decreased if the primer over-amplified itstemplate (FIG. 3). The adjusted mixture of amplification primers wasthen used in a PCR to amplify the template composition containing, inequimolar amounts, the set of 689 template oligonucleotides (SEQ IDNOS:872-1560) that were used to generate the data in FIGS. 2 and 3.

Amplification and quantitative sequencing were performed as describedabove and the results are shown in FIG. 4, which compares the frequencyat which each indicated amplified V region sequence-containing productwas obtained when all amplification primers were present at equimolarconcentrations (black bars) to the frequency at which each such productwas obtained after the concentrations of the amplification primers wereadjusted (grey bars) to the concentrations as indicated in Table 6.

Additional hs-TCRB primer sequences are found at SEQ ID NOs. 6192-6264.

Example 3 Correcting Non-Uniform Amplification Potential (PCR Bias) inTCR-Amplifying Oligonucleotide Primer Sets

Diverse TCR amplification primers are designed to amplify every possiblecombination of rearranged TCR V and J gene segments in a biologicalsample that contains lymphoid cell DNA from a subject. A preparationcontaining equimolar concentrations of the diverse amplification primersis used in multiplexed PCR to amplify a diverse template compositionthat comprises equimolar concentrations of TCR-specific templateoligonucleotides according to formula (I) with at least one templaterepresenting every possible V−J combination for the TCR locus. Theamplification products are quantitatively sequenced and the frequency ofoccurrence of each unique V−J product sequence is obtained from thefrequency of occurrence of each 16 bp molecular barcode sequence (B informula (I)) that uniquely identifies each V−J combination.

For TCRG, the TCRG template oligonucleotides (SEQ ID NOS:1561-1630) areamplified using TCRG V- and J-specific primers (SEQ ID NOS:1732-1745,Table 4). J primer independence of respectively paired V primers isidentified by separately amplifying each of the eight TCRG V genesegment specific primers with a pool of the five J gene segment specificprimers. The amplification products are quantitatively sequenced on anIllumina HiSeg™ sequencing platform and the frequency of occurrence ofthe internal 16 bp barcode sequences (B) that uniquely identify specificV−J combinations permit quantification of each V−J pair. V primerindependence of respectively paired J primers is identified byperforming the inverse reaction, i.e., by separately amplifying each ofthe five TCRG J gene segment primers with a pool of the eight V genesegment specific primers.

To test if TCRG V primers or J primers cross-amplify (e.g., whether genesegment specific primers amplify non-specifically, for instance, to testif the V primer specifically designed to amplify TCRG V7 segments isable to amplify both TCRG V6 and TCRG V7 V gene segments), independentprimer pools are generated that contain equimolar concentrations of allbut one of the primers, and the omitted primer is then added to the poolat twice the molar concentration of all other primers. The primers arethen used to amplify a template composition that comprises a pluralityof template oligonucleotides of general formula (I) as described herein,using TCRG V and J gene sequences in, respectively, the V and Jpolynucleotides of formula (I). Quantitative sequencing reveals theidentities of any one or more templates that are over represented amongthe amplification products when a single amplification primer is presentat twice the concentration of all other primers in the pool of primers.The primer mixture is then adjusted to increase or decrease the relativeconcentrations of one or more primers, to obtain amplificationfrequencies in iterative rounds that are within acceptable quantitativetolerances. The adjusted primer mixture so obtained is regarded ashaving been corrected to reduce non-uniform amplification potentialamong the members of the primer set.

To determine whether a corrected primer mixture exhibits unbiasedamplification potential when used to amplify rearranged TCR template DNAin a biological sample from lymphoid cells of a subject, the artificialtemplate compositions as described herein are prepared with all VJ pairspresent at similar frequency, and also with varying ratios of therelative representation of certain VJ pairs. Each type of templatepreparation is separately tested as an amplification template for anamplification primer set that has been corrected to reduce non-uniformamplification potential among certain members of the primer set.Quantitative sequence determination of amplification products identifiesthat the relative quantitative representation of specific sequences inthe template preparation is reflected in the relative quantitativerepresentation of specific sequences among the amplification products.

As an alternative to the iterative process described above, or inaddition to such iterative amplification steps followed by quantitativesequencing, amplification bias can also be corrected computationally.According to this computational approach, the starting frequency of eachof the species of template oligonucleotide sequences in the synthesizedtemplate composition is known. The frequency of each of these species ofoligonucleotide sequences among the amplification products that areobtained following PCR amplification is determined by quantitativesequencing. The difference between the relative frequencies of thetemplate oligonucleotide sequences prior to PCR amplification and theirfrequencies following PCR amplification is the “PCR bias.” Thisdifference is the amplification bias introduced during amplification,for example, as a consequence of different amplification efficienciesamong the various amplification primers.

As quantitatively determined for each known template oligonucleotidesequence, the PCR bias for each primer is used to calculate anamplification bias (normalization) factor by which the observedfrequency for each amplification product is corrected to reflect theactual frequency of the respective template sequence in the templatecomposition. If PCR bias for an amplification primer set is empiricallydetected using the present template composition as being within a factorof 10, then the bias can be computationally corrected in amplificationproducts obtained when the same amplification primer set is used toamplify a DNA sample of unknown composition. Improved accuracy in thequantification of template species in the DNA sample is therebyobtained.

Because V and J primers are empirically tested and shown to beindependent, an amplification bias factor can be derived for each Vspecies and for each J species, and an amplification factor for each VJspecies pair is not necessary. Accordingly, the amplification biasfactor for each V species and J species is derived using the presenttemplate composition. By the present method, the frequencies of the Vand J gene sequences in the template composition are known (or can becalculated based on knowledge of the concentrations of each templateoligonucleotide species in the template composition as synthesized)prior to PCR amplification. After PCR amplification, quantitativesequencing is used to detect the frequency of each V and J gene segmentsequence in the amplification products. For each sequence, thedifference in gene segment frequency is the amplification bias:

Initial Frequency/final frequency=amplification bias factor

Amplification bias factors are calculated for every V gene segment andevery J gene segment. These amplification factors, once calculated, canbe applied to samples for which the starting frequency of V and J genesis unknown.

In a mixed template population (such as a complex DNA sample obtainedfrom a biological source that comprises DNA from lymphoid cells that arepresumed to contain rearranged adaptive immune receptor encoding DNA, ora complex DNA sample which additionally comprises DNA from other cellslacking such rearrangements), where the starting frequency of each V andJ gene segment is unknown, the calculated amplification factors for aprimer set that has been characterized using the present templatecomposition can be used to correct for residual PCR amplification bias.For each species of sequenced amplification product molecule, the V andJ genes that are used by the molecule are determined based on sequencesimilarity. To correct for amplification bias, the number of times themolecule was sequenced is multiplied by both the correct V and Jamplification factors. The resulting sequence count is thecomputationally “normalized” set.

Example 4 Generation of Additional Template Compositions

Additional template compositions were designed and produced essentiallyaccording to the methodologies described above.

V and J Polynucleotides. TCRB V and J polynucleotide sequences weregenerated for inclusion in the herein described plurality of templateoligonucleotides and are set forth in sets of 68 TCRB V and J SEQ IDNOS, respectively, as shown in FIGS. 5a-5l as TCRB V/J set 1, TCRB V/Jset 2, TCRB V/J set 3, TCRB V/J set 4, TCRB V/J set 5, TCRB V/J set 6,TCRB V/J set 7, TCRB V/J set 8, TCRB V/J set 9, TCRB V/J set 10, TCRBV/J set 11, TCRB V/J set 12 and TCRB V/J set 13.

TCRG V and J polynucleotide sequences were generated for inclusion inthe herein described plurality of template oligonucleotides and are setforth in sets of 14 TCRG V and J SEQ ID NOS, respectively, as set forthin FIGS. 6a-6b as TCRG V/J set 1, TCRG V/J set 2, TCRG V/J set 3, TCRGV/J set 4 and TCRG V/J set 5.

IGH V and J polynucleotide sequences were generated for inclusion in theherein described plurality of template oligonucleotides and are setforth in sets of 127 IGH V and J SEQ ID NOS, respectively, as set forthin FIGS. 7a-7m as IGH V/J set 1, IGH V/J set 2, IGH V/J set 3, IGH V/Jset 4, IGH V/J set 5, IGH V/J set 6, IGH V/J set 7, IGH V/J set 8 andIGH V/J set 9.

Template Compositions. A template composition was prepared forstandardizing the amplification efficiency of TCRB amplification primersets. The composition comprised a plurality of template oligonucleotideshaving a plurality of oligonucleotide sequences of general formula (I).The TCRB template composition comprising 858 distinct templateoligonucleotides is disclosed in the Sequence Listing in SEQ IDNOS:3157-4014.

A template composition was prepared for standardizing the amplificationefficiency of TCRG amplification primer sets. The composition compriseda plurality of template oligonucleotides having a plurality ofoligonucleotide sequences of general formula (I). The TCRG templatecomposition comprising 70 distinct template oligonucleotides isdisclosed in the Sequence Listing in SEQ ID NOS:4015-4084.

A template composition was prepared for standardizing the amplificationefficiency of IGH amplification primer sets. The composition comprised aplurality of template oligonucleotides having a plurality ofoligonucleotide sequences of general formula (I). The IGH templatecomposition comprising 1116 distinct template oligonucleotides isdisclosed in the Sequence Listing in SEQ ID NOS:4085-5200. An IGHtemplate composition comprising a set of 1116 template oligonucleotidesis also disclosed in the Sequence Listing in SEQ TD NOS:1805-2920.

Example 5 Use of the Template Composition to Determine AmplificationFactor

This example describes quantification of rearranged DNA moleculesencoding a plurality of IG molecules, using the presently describedtemplate oligonucleotide composition as a “spiked-in” synthetic templatein a multiplexed PCR amplification of a DNA sample containing B cell andfibroblast DNA.

Biological Template DNA: Eight biological samples were used as sourcesof template DNA, with each biological sample containing the same amountof total genomic DNA (gDNA), 300 ng, but in a different proportion of(i) DNA extracted from B cells to (ii) DNA extracted from humanfibroblast cells, a cell type in which IG and TCR encoding genes do notrearrange. The samples contained 0, 0.07, 0.3, 1, 4, 18, 75 or 300 ng Bcell gDNA, with fibroblast gDNA supplying the balance of each 300 nggDNA preparation. Four replicates of each sample were made.

Synthetic Template DNA: To each PCR reaction (below) were added 5000molecules (4-5 molecules of each sequence) from an oligonucleotidetemplate composition comprising a pool of 1116 synthetic IGH templateoligonucleotide molecules (SEQ ID NOS:4085-5200). An IGH templatecomposition comprising a set of 1116 template oligonucleotides is alsodisclosed in the Sequence Listing as SEQ ID NOS:1805-2920.

PCR Reaction: The PCR reaction used QIAGEN Multiplex Plus™ PCR mastermix (QIAGEN part number 206152, Qiagen, Valencia, Calif.), 10%Q-solution (QIAGEN), and 300 ng of biological template DNA (describedabove). The pooled amplification primers were added so the finalreaction had an aggregate forward primer concentration of 2 μM and anaggregate reverse primer concentration of 2 μM. The forward primers (SEQID NOS:5201-5286) included 86 primers that had at the 3′ end anapproximately 20 bp segment that annealed to the IGH V segment encodingsequence and at the 5′ end an approximately 20 bp universal primerpGEXf. The reverse primers (SEQ ID NOS:5287-5293) included an aggregateof J segment specific primers that at the 3′ end had an approximately 20bp segment that annealed to the IGH J segment encoding sequence and atthe 5′ end of the J primers was a universal primer pGEXr. The followingthermal cycling conditions were used in a C100 thermal cycler (Bio-RadLaboratories, Hercules, Calif., USA): one cycle at 95° C. for 10minutes, 30 cycles at 94° C. for 30 seconds, 63° C. for 30 seconds, and72° C. for one minute, followed by one cycle at 72° C. for 10 minutes.Each reaction was run in quadruplicates.

For sequencing, Illumina adapters (Illumina Inc., San Diego, Calif.),which also included a 8 bp tag and a 6 bp random set of nucleotides,were incorporated onto the ends of the PCR reaction products in a 7cycle PCR reaction. The PCR reagents and conditions were as describedabove, except for the thermocycle conditions, which were: 95° C. for 5minutes, followed by 7 cycles of 95° for 30 sec, 68° for 90 sec, and 72°for 30 sec. Following thermal cycling, the reactions were held for 10minutes at 72° and the primers were the Illumina adaptor tailing primers(SEQ ID NOS:5387-5578). Samples were sequenced on an Illumina MiSEQ™sequencer using the Illumina PE RD2 primer.

Results: Sequence data were obtained for each sample and amplificationproducts of synthetic templates were identified by the presence of thebarcode oligonucleotide sequence. For each sample, the number oftemplate products was divided by the number of unique synthetic templateoligonucleotide sequences (1116) to arrive at a sample amplificationfactor. The total number of amplification products of the biologicaltemplates for each sample was then divided by the amplification factorto calculate the number of rearranged biological template molecules(e.g., VDJ recombinations) in the starting amplification reaction as anestimate of the number of unique B cell genome templates. The averagevalues with standard deviations were plotted against the known number ofrearranged biological template molecules based on B cell input (FIG. 9).In FIG. 9, the dots represent the average amplification factor and thebars represent the standard deviation across the four replicates. Theuse of amplification factors calculated as described herein to estimatethe number of VJ-rearranged IG encoding molecules (as a proxy value forthe number of B cells) yielded determinations that were consistent withknown B cell numbers at least down to an input of 100 B cells. Theestimated amplification factor values and the observed amplificationfactor were highly correlated (FIG. 9, R²=0.9988).

Example 6 IgH, IgL, and IgK BIAS CONTROL TEMPLATES

IgH VJ Template Oligonucleotides

In one embodiment, IgH VJ template oligonucleotides were generated andanalyzed. A set of 1134 template oligonucleotides of general formula (I)was designed using human IgH V and J polynucleotide sequences. Eachtemplate oligonucleotide consisted of a 495 base pair DNA molecule.Details for the 1134-oligonucleotide set of IgH templates arerepresentative and were as follows.

Based on previously determined genomic sequences, the human IgH locuswas shown to contain 126 Vh segments that each had a RSS sequence andwere therefore regarded as rearrangement-competent. These 126 Vhsegments included 52 gene segments known to be expressed, five Vsegments that were classified as having open reading frames, and 69 Vpseudogenes. The Vh gene segments were linked to 9 Jh gene segments. Inorder to include all possible V+J gene combinations for the 126 V and 9J segments, 1134 (9×126) templates were designed that represented allpossible VJ combinations. Each template conformed to the general formula(I) (5′-U1-B1-V-B2-R-J-B4-U2-3′)(FIG. 1) and thus included ninesections, a 19 base pair (bp) universal adapter (U1), a 16 bp nucleotidetag uniquely identifying each paired combination of V gene and J genesegments (B1), 300 bp of V gene specific sequence (V), a 3 bp stop codon(S), another copy of the 16 bp nucleotide tag (B2), a 6 bp junction tagshared by all molecules (R), nothing for B3, 100 bp of J gene specificsequence (J), a third copy of the 16 bp nucleotide tag (B4), and a 19 bpuniversal adapter sequence (U2). Two V segments were nucleotideidentical to another two V segments—and thus were not ordered. Thisreduced the number of included segments from 1134 to 1116. The IGHtemplate composition comprising 1116 distinct template oligonucleotidesis disclosed in the Sequence Listing in SEQ ID NOS:4085-5200.

Each of the 1116 templates was amplified individually usingoligonucleotide primers designed to anneal to the universal adaptersequences (U1, U2). These oligonucleotide sequences can be any universalprimer. For this application a universal primer coded Nextera was used.

TABLE 8 Universal Primer sequences included in bias control templatesPrimer Name Primer Sequence SEQ ID NO pGEXF GGGCTGGCAAGCCACGTTTGGTGSEQ ID NO: 6428 pGEXR CCGGGAGCTGCATGTGTCAGAGG SEQ ID NO: 6429

The universal primer sequences can be annealed to any primer sequencedisclosed herein. An example of the PCR primers including the universalprimer sequence are shown below:

TABLE 9 Example IGH PCR primers with UniversalSequences (Bold and Underlined) SEQ Primer Name Primer Sequence ID NOpGEXf_IGHV(II)- GGGCTGGCAAGCCACGTTTGGTG AG SEQ ID 15-1_ver10_01CCCCCAGGGAAGAAGCTGAAGTGG NO: 6430 pGEXr_IGHJ1/4/ CCGGGAGCTGCATGTGTCAGAGGCAC SEQ ID 5_ver10_03 CTGAGGAGACGGTGACCAGGGT NO: 6431

The resulting concentration of each amplified template oligonucleotideproduct was quantified using a LabChip GX™ capillary electrophoresissystem (Caliper Life Sciences, Inc., Hopkinton, Mass.) according to themanufacturer's instructions. The 1116 amplified template oligonucleotidepreparations were normalized to a standard concentration and thenpooled.

To verify that all 1116 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeq™ sequencing platform according to the manufacturer'srecommendations. To incorporate platform-specific oligonucleotidesequences into the pooled template oligonucleotides, tailed primers weredesigned that annealed to the universal priming sites (U1, U2) and thathad Illumina™ adapter sequence tails as the 5′ ends. A seven-cycle PCRreaction was then performed to anneal the Illumina adapters to thetemplate oligonucleotides. The PCR reaction product mixture was thenpurified using Agencourt® AMPure® XP beads (Beckman Coulter, Inc.,Fullerton, Calif.) under the conditions recommended by the manufacturer.The first 29 bp of the PCR reaction products were sequenced using anIllumina MiSEQ™ sequencer (Illumina, Inc., San Diego, Calif.) andanalyzed by assessing the frequency of each 16 bp molecular barcode tag(B1).

A substantially equimolar preparation for the set of 1116 distincttemplate oligonucleotides was calculated to contain approximately ˜0.09%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (0.009%-0.9%) for all species was desired. Thequantitative sequencing revealed that the 1116 species ofadapter-modified template oligonucleotides within the initial pool werenot evenly represented.

Accordingly, adjustment of the concentrations of individual templateoligonucleotides and reiteration of the quantitative sequencing stepsare conducted until each molecule is present within the thresholdtolerance concentration (0.009-0.9%).

IgH DJ Template Oligonucleotides

In another embodiment, IgH DJ template oligonucleotides were generatedand analyzed. A set of 243 template oligonucleotides of general formula(I) was designed using human IgH D and J polynucleotide sequences. Eachtemplate oligonucleotide consisted of a 382 base pair DNA molecule. TheIgH DJ template oligonucleotide sequences are presented in SEQ ID NOs:5579-5821. Details for the 243-oligonucleotide set of IgH templates arerepresentative and were as follows.

Based on previously determined genomic sequences, the human IgH locuswas shown to contain 27 Dh segments. The 27 Dh gene segments were linkedto 9 Jh gene segments. To include all possible D+J gene combinations forthe 27 D and 9 J segments, 243 (9×27) templates were designed thatrepresented all possible DJ combinations. Each template conformed to thegeneral formula (I) (5′-U1-B1-V-B2-R-J-B4-U2-3′) (FIG. 1) and thusincluded nine sections, a 19 base pair (bp) universal adapter (U1), a 16bp nucleotide tag uniquely identifying each paired combination of D geneand J gene segments (B1). However, for these molecules, the 300 bp of Vgene specific sequence (V) was replaced with a segment of 182 bp of Dgene specific sequence. This segment included both exonic and intronicnucleotide segments. Like the other molecules, these included a 3 basepair (bp) stop codon (S), another copy of the 16 bp nucleotide tag (B2),a 6 bp junction tag shared by all molecules (R), nothing for B3, 100 bpof J gene specific sequence (J), a third copy of the 16 bp nucleotidetag (B4), and a 19 bp universal adapter sequence (U2).

Each of the 243 templates (SEQ ID NOs: 5579-5821) was amplifiedindividually using oligonucleotide primers designed to anneal to theuniversal adapter sequences (U1, U2; See Table 8). These oligonucleotidesequences can be any universal primer; for this application a universalprimer coded Nextera was used.

An example of the PCR primers with the universal adapter sequences areshown in Table 10.

TABLE 10 Example IgH DJ PCR primers with UniversalSequences (Bold and Underlined) SEQ Primer Name Primer Sequence ID NOpGEXf_IGHV(II)- GGGCTGGCAAGCCACGTTTGGTG SEQ ID 15-1_ver10_01AGCCCCCAGGGAAGAAGCTGAAGTGG NO: 6432 pGEXr_IGHJ1/ CCGGGAGCTGCATGTGTCAGAGGSEQ ID 4/5_ver10_03 CACCTGAGGAGACGGTGACCAGGGT NO: 6433

The resulting concentration of each amplified template oligonucleotideproduct was quantified using a LabChip GX™ capillary electrophoresissystem (Caliper Life Sciences, Inc., Hopkinton, Mass.) according to themanufacturer's instructions. The 243 amplified template oligonucleotidepreparations were normalized to a standard concentration and thenpooled.

To verify that all 243 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeq™ sequencing platform according to the manufacturer'srecommendations. To incorporate platform-specific oligonucleotidesequences into the pooled template oligonucleotides, tailed primers weredesigned that annealed to the universal priming sites (U1, U2) and thathad Illumina™ adapter sequence tails as the 5′ ends. A seven-cycle PCRreaction was then performed to anneal the Illumina adapters to thetemplate oligonucleotides. The PCR reaction product mixture was thenpurified using Agencourt® AMPure® XP beads (Beckman Coulter, Inc.,Fullerton, Calif.) under the conditions recommended by the manufacturer.The first 29 bp of the PCR reaction products were sequenced using anIllumina MiSEQ™ sequencer (Illumina, Inc., San Diego, Calif.) andanalyzed by assessing the frequency of each 16 bp molecular barcode tag(B1).

A substantially equimolar preparation for the set of 243 distincttemplate oligonucleotides was calculated to contain approximately ˜0.4%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (0.04%-4.0%) for all species was desired. Thequantitative sequencing revealed that the 243 species ofadapter-modified template oligonucleotides within the initial pool werenot evenly represented.

Accordingly, adjustment of the concentrations of individual templateoligonucleotides and reiteration of the quantitative sequencing stepsare conducted until each molecule is present within the thresholdtolerance concentration (0.04-4.0%). Following normalization, this setwas combined with 1116 IgH VJ bias control set for a pool of 1359templates.

FIG. 10 shows results for a pre-PCR amplification sequencing count foreach of the 1116 IGH VJ bias control molecules and 243 IGH DJ biascontrol molecules. Individual bias control molecules are along thex-axis. The set includes the 1116 IGH VJ bias control molecules and 243IGH DJ bias control molecules for a total of 1359 gblocks. The Y axis isthe sequence count for each individual gblock. This calculation providesthe quantification of the composition of the pre-amplificationrepresentation of each VJ pair. This data is used to estimate the changein frequency between the pre-sample and post-PCR amplification sample tocalculate the amplification bias introduced by the primers.

IgL VJ Template Oligonucleotides

In another embodiment, IgL VJ template oligonucleotides were generatedand analyzed. A set of 245 template oligonucleotides of general formula(I) was designed using human IgL V and J polynucleotide sequences. Eachtemplate oligonucleotide consisted of a 495 base pair DNA molecule. TheIgL template oligonucleotides are presented as SEQ ID NOs: 5822-6066.Details for the 245-oligonucleotide set of IgL templates arerepresentative and were as follows.

Based on previously determined genomic sequences, the human IgL locuswas shown to contain 75 VL segments that each had a RSS sequence andwere therefore regarded as rearrangement-competent. These 33 VL segmentsincluded gene segments known to be expressed, 5 V segments that wereclassified as having open reading frames, and 37 V pseudogenes. The VLgene segments were linked to five 6 JL gene segments. To include allpossible functional and expressed V+J gene combinations for the 33functional V and 6 J segments, 204 (6×33) templates were designed thatrepresented all possible expressed VJ combinations. In addition, two ofthe V pseudogenes were questionable; an additional 12 (2×6) VJ templateswere designed, resulting in a total of 216. Each template conformed tothe general formula (I) (5′-U1-B1-V-B2-R-J-B4-U2-3′) (FIG. 1) and thusincluded nine sections, a 19 base pair (bp) universal adapter (U1), a 16bp nucleotide tag uniquely identifying each paired combination of V geneand J gene segments (B1), 300 bp of V gene specific sequence (V), a 3 bpstop codon (S), another copy of the 16 bp nucleotide tag (B2), a 6 bpjunction tag shared by all molecules (R), nothing for B3, 100 bp of Jgene specific sequence (J), a third copy of the 16 bp nucleotide tag(B4), and a 19 bp universal adapter sequence (U2).

Each of the 216 templates was amplified individually usingoligonucleotide primers designed to anneal to the universal adaptersequences (U1, U2). These oligonucleotide sequences can be any universalprimer; for this application, a universal primer coded Nextera was used.

The resulting concentration of each amplified template oligonucleotideproduct was quantified using a LabChip GX™ capillary electrophoresissystem (Caliper Life Sciences, Inc., Hopkinton, Mass.) according to themanufacturer's instructions. The 216 amplified template oligonucleotidepreparations were normalized to a standard concentration and thenpooled.

To verify that all 216 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeq™ sequencing platform according to the manufacturer'srecommendations. To incorporate platform-specific oligonucleotidesequences into the pooled template oligonucleotides, tailed primers weredesigned that annealed to the universal priming sites (U1, U2) and thathad Illumina™ adapter sequence tails as the 5′ ends. A seven-cycle PCRreaction was then performed to anneal the Illumina adapters to thetemplate oligonucleotides. The PCR reaction product mixture was thenpurified using Agencourt® AMPure® XP beads (Beckman Coulter, Inc.,Fullerton, Calif.) under the conditions recommended by the manufacturer.The first 29 bp of the PCR reaction products were sequenced using anIllumina MiSEQ™ sequencer (Illumina, Inc., San Diego, Calif.) andanalyzed by assessing the frequency of each 16 bp molecular barcode tag(B1).

A substantially equimolar preparation for the set of 216 distincttemplate oligonucleotides was calculated to contain approximately ˜0.46%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (0.046%-4.6%) for all species was desired. Thequantitative sequencing revealed that the 216 species ofadapter-modified template oligonucleotides within the initial poolevenly represented.

IgK VJ Template Oligonucleotides

In one embodiment, IgK VJ template oligonucleotides were generated andanalyzed. A set of 560 template oligonucleotides of general formula (I)was designed using human IgK V and J polynucleotide sequences. Eachtemplate oligonucleotide consisted of a 495 base pair DNA molecule.Examples of IgK template oligonucleotides are found at SEQ ID NOs:6067-6191. Details for the 560-oligonucleotide set of IgK templates arerepresentative and were as follows.

Based on previously determined genomic sequences, the human IgK locuswas shown to contain 112 Vk segments that each had a RSS sequence andwere therefore regarded as rearrangement-competent. These 112 Vksegments included 46 gene segments known to be expressed, 8 V segmentsthat were classified as having open reading frames, and 50 Vpseudogenes. For this IgK, only expressed IgK VJ rearrangements wereanalyzed. Genes classified as pseudogenes and open reading frames wereexcluded. The Vk gene segments were linked to five Jk gene segments.This left us with 230 VJ gene rearrangements (46×5). To include allpossible functional V+J gene combinations for the 46 functional V and 5J segments, 230 (5×46) templates were designed that represented allpossible VJ combinations. Each template conformed to the general formula(I) (5′-U1-B1-V-B2-R-J-B4-U2-3′) (FIG. 1) and thus included ninesections, a 19 base pair (bp) universal adapter (U1), a 16 bp nucleotidetag uniquely identifying each paired combination of V gene and J genesegments (B1), 300 bp of V gene specific sequence (V), a 3 bp stop codon(S), another copy of the 16 bp nucleotide tag (B2), a 6 bp junction tagshared by all molecules (R), nothing for B3, 100 bp of J gene specificsequence (J), a third copy of the 16 bp nucleotide tag (B4), and a 19 bpuniversal adapter sequence (U2).

Each of the 230 templates was amplified individually usingoligonucleotide primers designed to anneal to the universal adaptersequences (U1, U2). These oligonucleotide sequences can be any universalprimer—for this application a universal primer coded Nextera was used.

The resulting concentration of each amplified template oligonucleotideproduct was quantified using a LabChip GX™ capillary electrophoresissystem (Caliper Life Sciences, Inc., Hopkinton, Mass.) according to themanufacturer's instructions. The 230 amplified template oligonucleotidepreparations were normalized to a standard concentration and thenpooled.

To verify that all 230 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeq™ sequencing platform according to the manufacturer'srecommendations. Briefly, to incorporate platform-specificoligonucleotide sequences into the pooled template oligonucleotides,tailed primers were designed that annealed to the universal primingsites (U1, U2) and that had Illumina™ adapter sequence tails as the 5′ends. A seven-cycle PCR reaction was then performed to anneal theIllumina adapters to the template oligonucleotides. The PCR reactionproduct mixture was then purified using Agencourt® AMPure® XP beads(Beckman Coulter, Inc., Fullerton, Calif.) under the conditionsrecommended by the manufacturer. The first 29 bp of the PCR reactionproducts were sequenced using an Illumina MiSEQ™ sequencer (Illumina,Inc., San Diego, Calif.) and analyzed by assessing the frequency of each16 bp molecular barcode tag (B1).

A substantially equimolar preparation for the set of 230 distincttemplate oligonucleotides was calculated to contain approximately ˜0.4%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (4.0%-0.04%) for all species was desired. Thequantitative sequencing revealed that the 230 species ofadapter-modified template oligonucleotides within the initial pool wereevenly represented.

Example 7 Combined Assays

IgH DJ and IgH VJ Combined Assay

In some embodiments, it is desired to co-amplify and sequence rearrangedIgH VDJ CDR3 chains and rearranged IgH DJ chains. To generate a pool oftemplates to test a combined IgH DJ and IgH VJ assay using the IgH DJand IgH VJ templates. When pooled-, the final pool includes 1116 VJ and243 DJ templates, resulting in a total of 1359 individual templates. TheIgH VJ template composition comprising 1116 distinct templateoligonucleotides is disclosed in the Sequence Listing in SEQ ID NOs:4085-5200. The IgH DJ template oligonucleotide sequences are presentedin SEQ ID NOs: 5579-5821.

To verify that all 1359 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeg™ sequencing platform according to the manufacturer'srecommendations. To incorporate platform-specific oligonucleotidesequences into the pooled template oligonucleotides, tailed primers weredesigned that annealed to the universal priming sites (U1, U2) and thathad Illumina™ adapter sequence tails as the 5′ ends. A seven-cycle PCRreaction was then performed to anneal the Illumina adapters to thetemplate oligonucleotides. The PCR reaction product mixture was thenpurified using Agencourt® AMPure® XP beads (Beckman Coulter, Inc.,Fullerton, Calif.) under the conditions recommended by the manufacturer.The first 29 bp of the PCR reaction products were sequenced using anIllumina MiSEQ™ sequencer (Illumina, Inc., San Diego, Calif.) andanalyzed by assessing the frequency of each 16 bp molecular barcode tag(B1).

A substantially equimolar preparation for the set of 1359 distincttemplate oligonucleotides was calculated to contain approximately˜0.073% of each member of the set, and a threshold tolerance of plus orminus ten-fold frequency (0.73%-0.0073%) for all species was desired.The quantitative sequencing revealed that the 1359 species ofadapter-modified template oligonucleotides within the initial pool wereevenly represented.

IgL and IgK Combined Assay

In other embodiments, it is desired to co-amplify and sequencerearranged IgL and IgK rearranged CDR3 chains. To generate a pool oftemplates to test a combined IgL and IgK assay (the IgL and IgKtemplates were combined). When pooled, the final pool includes 216 IgLand 230 IgK templates, for a total of 446 individual templates. The IgLtemplate oligonucleotides are presented as SEQ ID NOs: 5822-6066.

To verify that all 446 template oligonucleotides were present atsubstantially equimolar concentrations, the pool was sequenced using theIllumina MiSeg™ sequencing platform according to the manufacturer'srecommendations. Briefly, to incorporate platform-specificoligonucleotide sequences into the pooled template oligonucleotides,tailed primers were designed that annealed to the universal primingsites (U1, U2) and that had Illumina™ adapter sequence tails as the 5′ends. A seven-cycle PCR reaction was then performed to anneal theIllumina adapters to the template oligonucleotides. The PCR reactionproduct mixture was then purified using Agencourt® AMPure® XP beads(Beckman Coulter, Inc., Fullerton, Calif.) under the conditionsrecommended by the manufacturer. The first 29 bp of the PCR reactionproducts were sequenced using an Illumina MiSEQ™ sequencer (Illumina,Inc., San Diego, Calif.) and analyzed by assessing the frequency of each16 bp molecular barcode tag (B1).

A substantially equimolar preparation for the set of 446 distincttemplate oligonucleotides was calculated to contain approximately ˜0.22%of each member of the set, and a threshold tolerance of plus or minusten-fold frequency (2.2%-0.022%) for all species was desired. Thequantitative sequencing revealed that the 446 species ofadapter-modified template oligonucleotides within the initial pool wereevenly represented.

Example 8 Correcting Non-Uniform Amplification Potential (PCR Bias) inIGH-Amplifying Oligonucleotide Primer Sets

Diverse IgH amplification primers were designed to amplify everypossible combination of rearranged IgH V and J gene segments in abiological sample that contains lymphoid cell DNA from a subject. Apreparation containing equimolar concentrations of the diverseamplification primers was used in multiplexed PCR to amplify a diversetemplate composition that comprises equimolar concentrations ofIgH-specific template oligonucleotides according to formula (I) with atleast one template representing every possible V−J combination for theIgH locus. The amplification products were quantitatively sequenced andthe frequency of occurrence of each unique V−J product sequence wasobtained from the frequency of occurrence of each 16 bp molecularbarcode sequence (B in formula (I)) that uniquely identified each V−Jcombination.

The multiplex PCR reaction was designed to amplify all possible V and Jgene rearrangements of the IgH locus, as annotated by the IMGTcollaboration. See Yousfi Monod M, Giudicelli V, Chaume D, Lefranc. MP.IMGT/JunctionAnalysis: the first tool for the analysis of theimmunoglobulin and T cell receptor complex V−J and V-D-J JUNCTIONs.Bioinformatics. 2004; 20(suppl 1):i379-i385. The locus included 126unique V genes; 52 functional genes, 6 putative open reading frameslacking critical amino acids for function and 69 pseudogenes; and 9 Jgenes, 6 functional and 3 pseudogenes. The target sequence for primerannealing was identical for some V segments, allowing amplification ofall 126 V segments with 86 unique forward primers. Similarly, 7 uniquereverse primers annealed to all 9 J genes. As a baseline for biasassessment, the pool of 1116 templates was amplified using an equimolarpool of the 86 V forward primers (VF; specific to V genes) and anequimolar pool of the 7 J reverse primers (JR; specific to J genes).

Polymerase chain reactions (PCR) (25 μL each) were set up at 2.0 μM VF,2.0 μM JR pool (Integrated DNA Technologies), 1 μM QIAGEN Multiplex PlusPCR master mix (QIAGEN, Valencia, Calif.), 10% Q-solution (QIAGEN), and200,000 target molecules from our synthetic IgH repertoire mix. Thefollowing thermal cycling conditions were used in a C100 thermal cycler(Bio-Rad Laboratories, Hercules, Calif.): one cycle at 95° C. for 6minutes, 31 cycles at 95° C. for 30 see, 64° C. for 120 sec, and 72° C.for 90 sec, followed by one cycle at 72° C. for 3 minutes. For allexperiments, each PCR condition was replicated three times.

Following initial bias assessment, experiments were performed to defineall individual primer amplification characteristics. To determine thespecificity of VF and JR primers, 86 mixtures were prepared containing asingle VF primer with all JR primers, and 7 mixtures containing a singleJR primer with all VF primers. These primer sets were used to amplifythe synthetic template and sequenced the resulting libraries to measurethe specificity of each primer for the targeted V or J gene segments,and to identify instances of off-target priming. Titration experimentswere performed using pools of 2-fold and 4-fold concentrations of eachindividual VF or JF within the context of all other equimolar primers(e.g. 2×-fold IgHV1-01+ all other equimolar VF and JR primers) toestimate scaling factors relating primer concentration to observedtemplate frequency.

Primer Mix Optimization

Using the scaling factors derived by titrating primers one at a time,alternative primer mixes were developed in which the primers werecombined at uneven concentrations to minimize amplification bias. Therevised primer mixes were then used to amplify the template pool andmeasure the residual amplification bias. This process was reiterated,reducing or increasing each primer concentration appropriately based onwhether templates amplified by that primer were over orunder-represented in the previous round of results. At each stage ofthis iterative process, the overall degree of amplification bias wasdetermined by calculating metrics for the dynamic range (max bias/minbias) and sum of squares (SS, calculated on log(bias) values), anditerated the process of adjusting primer concentrations until there wasminimal improvement between iterations. To assess the robustness of thefinal optimized primer mix and scaling factors to deviations fromequimolar template input, we used a highly uneven mixture of IgHreference templates to determine the effect on sequencing output. Thefinal mix was substantially better than an equimolar mix.

Example 9 Correcting Non-Uniform Amplification Potential (PCR Bias) inTCRB-Amplifying Oligonucleotide Primer Sets

Diverse TCRB amplification primers were designed to amplify everypossible combination of rearranged TCRB V and J gene segments in abiological sample that contains lymphoid cell DNA from a subject. Apreparation containing equimolar concentrations of the diverseamplification primers was used in multiplexed PCR to amplify a diversetemplate composition that comprises equimolar concentrations ofTCRB-specific template oligonucleotides according to formula (I) with atleast one template representing every possible V−J combination for theTCRB locus. The amplification products were quantitatively sequenced andthe frequency of occurrence of each unique V−J product sequence wasobtained from the frequency of occurrence of each 16 bp molecularbarcode sequence (B in formula (I)) that uniquely identifies each V−Jcombination.

The multiplex PCR reaction was designed to amplify all possible V and Jgene rearrangements of the TCRB locus, as annotated by the IMGTcollaboration. See Yousfi Monod M, Giudicelli V, Chaume D, Lefranc. MP.IMGT/JunctionAnalysis: the first tool for the analysis of theimmunoglobulin and T cell receptor complex V−J and V-D-J JUNCTIONs.Bioinformatics. 2004; 20(suppl 1):i379-i385. The locus includes 67unique V genes. The target sequence for primer annealing was identicalfor some V segments, allowing us to amplify all 67 V segments with 60unique forward primers. For the J locus, 13 unique reverse primersannealed to 13 J genes. As a baseline for bias assessment, the pool of868 templates was amplified using an equimolar pool of the 60 V forwardprimers (VF; specific to V genes) and an equimolar pool of the 13 Jreverse primers (JR; specific to J genes). Polymerase chain reactions(PCR) (25 μL each) were set up at 3.0 μM VF, 3.0 μM JR pool (IntegratedDNA Technologies), 1 μM QIAGEN Multiplex Plus PCR master mix (QIAGEN,Valencia, Calif.), 10% Q-solution (QIAGEN and 200,000 target moleculesfrom our synthetic TCRB repertoire mix. The following thermal cyclingconditions were used in a 0100 thermal cycler (Bio-Rad Laboratories,Hercules, Calif.): one cycle at 95° C.′ for 5 minutes, 31 cycles at 95°C. for 30 sec 62° C. for 90 sec. and 72° C. for 90 sec, followed by onecycle at 72° C. for 3 minutes. For all experiments, each PCR conditionwas replicated three times.

Following initial bias assessment, experiments were performed to defineall individual primer amplification characteristics. To determine thespecificity of our VF and JR primers, 60 mixtures were preparedcontaining a single VF primer with all JR primers, and 13 mixturescontaining a single JR primer with all VF primers. These primer setswere used to amplify the synthetic template and sequenced the resultinglibraries to measure the specificity of each primer for the targeted Vor J gene segments and to identify instances of off-target priming.Titration experiments were performed using pools of 2-fold and 4-foldconcentrations of each individual VF or JF within the context of allother equimolar primers (e.g. 2×-fold TCRBV07-6+ all other equimolar VFand JR primers) to allow us to estimate scaling factors relating primerconcentration to observed template frequency.

Primer Mix Optimization

Using the scaling factors derived by titrating primers one at a time,alternative primer mixes were developed in which the primers werecombined at uneven concentrations to minimize amplification bias. Therevised primer mixes were then used to amplify the template pool andmeasure the residual amplification bias. This process was iterated,reducing or increasing each primer concentration appropriately based onwhether templates amplified by that primer were over orunder-represented in the previous round of results. At each stage ofthis iterative process, the overall degree of amplification bias wasdetermined by calculating metrics for the dynamic range (max bias/minbias) and sum of squares (SS, calculated on log(bias) values), anditerated the process of adjusting primer concentrations until there wasminimal improvement between iterations. The final mix was substantiallybetter than an equimolar mix of primers.

FIG. 11 shows TCRB-primer iterations for synthetic TCRB VJ templatesgraphed against relative amplification bias. Relative amplification biaswas determined for 858 synthetic TCRB VJ templates prior to chemicalbias control correction (Equimolar Primers (black)), post chemicalcorrection (Optimized Primers (dark grey)), and post chemical andcomputational correction (After computational adjustment (light grey)).The equimolar primers had a dynamic range of 264, an interquartile rangeof 0.841, and a sum of squares (log bias) of 132. The optimized primershad a dynamic range of 147, an interquartile range of 0.581, and a sumof squares (log bias) of 50.7. The corrected primers (aftercomputational adjustment) had a dynamic range of 90.8, an interquartilerange of 0.248, and a sum of squares (log bias) of 12.8.

Example 10 Correcting Non-Uniform Amplification Potential (PCR Bias) ina Combined IGH VJ and DJ-Amplifying Oligonucleotide Primer Sets

Diverse IgH amplification primers were designed to amplify everypossible combination of rearranged IgH V and J gene segments and IgH Dand J gene segments in a biological sample that contained lymphoid cellDNA from a subject. A preparation containing equimolar concentrations ofthe diverse amplification primers was used in multiplexed PCR to amplifya diverse template composition that comprises equimolar concentrationsof IgH-specific template oligonucleotides according to formula (I) withat least one template representing every possible V−J combination forthe IgH locus and every possible D-J combination for the IgH locus. Theamplification products were quantitatively sequenced and the frequencyof occurrence of each unique V−J and D-J product sequence was obtainedfrom the frequency of occurrence of each 16 bp molecular barcodesequence (B in formula (I)) that uniquely identifies each V−J and D-Jcombination.

The multiplex PCR reaction was designed to amplify all possible V and Jgene rearrangements AND D and J gene rearrangements of the IgH locus, asannotated by the IMGT collaboration. The locus included 126 unique Vgenes; 52 functional genes, 6 putative open reading frames lackingcritical amino acids for function and 69 pseudogenes; and 9 J genes, 6functional and 3 pseudogenes. The locus also included 27 unique D genes.The target sequence for primer annealing was identical for some Vsegments, allowing amplification of all 126 V segments with 86 uniqueforward primers. Similarly, 7 unique reverse primers annealed to all 9 Jgenes. For the D-J assay, primers were designed to anneal to rearranged−DJ stems. During B cell development, both alleles undergo rearrangementbetween the D and J gene segments, resulting in two −DJ stems. A −DJstem includes a J gene, one N region, and a D gene. Following DJrearrangements, one of the two alleles V gene rearranges with the −DJstem to code for the CDR3 gene region (VnDnJ). To amplify the −DJ stem,27 unique primers were designed to anneal to each specific D genes in anintronic region upstream of the D gene exon. These segments, whilepresent in −DJ stems. are excised following V to −DJ recombination.However, J primers were not re-designed; the DJ assay used the same Jprimers as the VJ assay.

As a baseline for bias assessment, the pool of 1359 templates wasamplified using an optimized (mix 2-1) pool of the 86 V forward primers(VF; specific to V genes), 27 D forward primers (DF; specific to Dgenes) and an equimolar pool of the 7 J reverse primers (JR; specific toJ genes). Polymerase chain reactions (PCR) (25 μL each) were set up at1.0 VF, 1.0 μM DF, and 2.0 μM JR pool (Integrated DNA Technologies),1×QIAGEN Multiplex Plus PCR master mix (QIAGEN, Valencia, Calif.), 10%Q-solution (QIAGEN), and 200,000 target molecules from our synthetic IgHVJ and DJ repertoire mix. The following thermal cycling conditions wereused in a C100 thermal cycler (Bio-Rad Laboratories, Hercules, Calif.):one cycle at 95° C. for 6 minutes, 31 cycles at 95° C. for 30 sec, 64°C. for 120 sec, and 72° C. for 90 sec, followed by one cycle at 72° C.for 3 minutes. For all experiments, each PCR condition was replicatedthree times.

Following initial bias assessment, experiments were performed to defineall individual primer amplification characteristics. To determine thespecificity of our DF and JR primers, 27 mixtures were preparedcontaining a single DF primer with all JR primers and the previouslyidentified optimized VF pool, and 7 mixtures containing a single JRprimer with all VF and DF primers. These primer sets were used toamplify the synthetic template and sequenced the resulting libraries tomeasure the specificity of each primer for the targeted V, D, or J genesegments, and to identify instances of off-target priming.

Titration experiments were performed using pools of 2-fold and 4-foldconcentrations of each individual DF or JF within the context of allother primers—including the optimized mix of VF primers (e.g. 2×-foldIgHD2-08+ all other equimolar DF, optimal VF mix, and JR primers) toallow us to estimate scaling factors relating primer concentration toobserved template frequency.

Primer Mix Optimization

Using the cross-amplification test, the DF primers were identified ascross amplified. 12 of the DF primers were removed, resulting in a finalpool of 15 DF primers. Using the scaling factors derived by titratingprimers one at a time, alternative primer mixes were developed in whichthe primers were combined at uneven concentrations to minimizeamplification bias. The revised primer mixes were then used to amplifythe template pool and measure the residual amplification bias. Thisprocess was iterated, reducing or increasing each primer concentrationappropriately based on whether templates amplified by that primer wereover or under-represented in the previous round of results. At eachstage of this iterative process, the overall degree of amplificationbias was determined by calculating metrics for the dynamic range (maxbias/min bias) and sum of squares (SS, calculated on log(bias) values),and iterated the process of adjusting primer concentrations until therewas minimal improvement between iterations. The final primer mix hassubstantially less primer bias than an equimolar primer mix.

FIG. 12 shows IGH primer iterations for synthetic IGH VJ templatesgraphed against relative amplification bias. Relative amplification biaswas determined for 1116 synthetic IGH VJ templates relativeamplification bias prior to chemical bias control correction (equimolarprimers (black)), post chemical correction (optimized primers (darkgrey)), and post chemical and computational correction (Aftercomputational adjustment (light grey)). The equimolar primers had adynamic range of 1130, an interquartile range of 0.991, and a sum ofsquares (log bias) of 233. The optimized primers had a dynamic range of129, an interquartile range of 0.732, and a sum of squares (log bias) of88.2. The after computational adjusted primers had a dynamic range of76.9, an interquartile range of 0.545, and a sum of squares (log bias)of 37.9.

FIG. 13 shows the relative amplification bias for 27 synthetic IGH DJtemplates of the V gene. Relative amplification bias of the V genesegment is shown in three primer iterations: 1) prior to chemical biascontrol correction (black), 2) a first iteration of chemical correction(white), and 3) a post second iteration of chemical correction (lightgrey).

Example 11 TCRG VJ Primer Iterations

In other embodiments, TCRG VJ primers were tested for relativeamplification bias in multiple primer iterations. FIGS. 14a-d showTCRG-primer iterations for 55 synthetic TCRG VJ templates. Relativeamplification bias was determined for the TCRG VJ primers prior tochemical bias control correction (FIG. 14a ), a first iteration ofchemical correction (FIG. 14b ), a second iteration of chemicalcorrection (FIG. 14c ), and final iteration of chemical correction (FIG.14d ).

Example 12 Alternative Bias Control and Spike-In Method

In other embodiments, alternative methods can be used to determineamplification bias. Two primary goals of the method are as follows: (1)to remove amplification bias in a multiplex PCR amplification of BCR orTCR genes and (2) to estimate the fraction of B or T cells in thestarting template.

The method includes starting with a set of cells comprising DNA, or cDNA(mRNA) extracted from a sample that includes B and/or T cells. In asample comprising cells, the DNA is extracted using methods standard inthe art.

The extracted DNA is divided into multiple parts and put into differentPCR wells. In some embodiments, one well is used to full capacity orthousands of wells can be used. In one embodiment, 188 wells are usedfor PCR (two 96 well plates). The number of TCR or BCR templates perwell should be sparse, such that it is rare to have multiple moleculesfrom the same clonotype in the same well.

The method then includes amplifying the DNA separately in each wellusing the same multiplex set of primers. The sets of primers describedherein can be used. As described above, the bar coding method is appliedto the amplified molecules in each well with the same barcode sequence.For example, each well gets its own barcode.

The molecules are then sequenced on a high throughput sequencing machinewith a sufficient amount of the amplified BCR or TCR sequences toidentify by sequence the V and the J chain used, as well as the bar codesequence.

Each well has an average copy count. Since each clonotype appears oncein a well, the amount of that template relative to the average is thebias for that V−J combination. Since V and J bias are independent, notevery V−J combination is necessary to determine the biases. Theserelative biases are then used to either re-design primers that areeither highly under or over amplifying or to titrate the primerconcentration to increase or decrease amplification. The entire processis repeated with a new primer lot and iterated to continue to decreasebias.

After any cycle of the iterations, a set of computational factors (therelative amplification factors) can be applied to remove bias. Bias canbe reduced by both (or either) primer changes and computationalcorrection.

The method includes computing a fraction of nucleated cells from asimilar assay. For each well, each clonotype is identified, and thenumber of sequencing reads is determined for each clone. In someembodiments, the number of templates does not need to be sparse. Theread counts for each clone are corrected by the bias control factors asdescribed above.

A histogram is created of the corrected read counts, and the graph has aprimary mode (the amplification factor). This mode is identified byinspection (based on identification of the first large peak), or byFourier transform, or other known methods.

The total number of corrected reads in each well is divided by theamplification factor for that well. This is the estimated number of TCRor BCR genome templates that were in the well. The total number of BCRor TCRs from the sample is the sum of the number from all the wells. Thetotal number of genomes in each well is measured prior to PCR. This canbe done by nanodrop, or other known methods used to quantify DNA. Themeasured weight of the DNA is divided by the weight of a double strandedgenome (for example, in humans ˜6.2 pico grams).

The fraction of B cells or T cells in the sample is the total number ofBCR or TCRs in the samples divided by the total number of doublestranded DNA molecules added to the reaction. The result needs a minorcorrection as a small fraction of T cells have both alleles rearranged.This correction factor is approximately 15% for alpha beta T cells, 10%for B cells. For gamma delta T cells, almost all of the cells have bothalleles rearranged, so the correction is a factor of two.

These additional methods can determine amplification bias in a multiplexPCR amplification of BCR or TCR genes and be used to estimate thefraction of B or T cells in the starting template.

The various embodiments described above can be combined to providefurther embodiments. All of the U.S. patents, U.S. patent applicationpublications, U.S. patent applications, foreign patents, foreign patentapplications and non-patent publications referred to in thisspecification and/or listed in the Application Data Sheet areincorporated herein by reference, in their entirety. Aspects of theembodiments can be modified, if necessary to employ concepts of thevarious patents, applications and publications to provide yet furtherembodiments.

These and other changes can be made to the embodiments in light of theabove-detailed description. In general, in the following claims, theterms used should not be construed to limit the claims to the specificembodiments disclosed in the specification and the claims, but should beconstrued to include all possible embodiments along with the full scopeof equivalents to which such claims are entitled. Accordingly, theclaims are not limited by the disclosure.

INFORMAL SEQUENCE LISTING Name Sequence SEQ ID NO Bias Control Sequencesfor hs-IgH-DJ (243 Sequences) hsIGH_2001_D001_J001_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTGTACAGTGGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ1TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5579CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGACACTCTGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2002_D002_J001_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACGTACAGTGGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ1GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5580ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTTCGGAACGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2003_D003_J001_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGGTACAGTGGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ1GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5581ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGAAGTAACGGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2004_D004_J001_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAGTACAGTGGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ1TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5582CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGTCTCCTAGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2005_D005_J001_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCGTACAGTGAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ1TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5583TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGAGAGTGTCGTACAGTGCTGATGGCGCGAGGGAGG ChsIGH_2006_D006_J001_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAGTACAGTGAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ1CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5584AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGTTCCGAAGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2007_D007_J001_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTGTACAGTGAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ1CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5585AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGCGTTACTTGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2008_D008_J001_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACGTACAGTGAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ1CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5586AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTAGGAGACGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2009_D009_J001_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACGTACAGTGAGCCCCCTGTACA SEQ ID NO: 21_IGHJ1GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5587ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGTGTCTACGTACAGTGCTGATGGCGC GAGGGAGGChsIGH_2010_D010_J001_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACGTACAGTGGTGGGCACGGACA SEQ ID NO: 03_IGHJ1CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5588GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTGCTACACGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2011_D011_J001_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAGTACAGTGTGGGCACGGACAC SEQ ID NO: 09_IGHJ1TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5589AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGAACTGCCAGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2012_D012_J001_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGGTACAGTGCGATATTTTGACT SEQ ID NO: 10_IGHJ1GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5590GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTTGGACTGGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2013_D013_J001_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACGTACAGTGTGGACGCGGACAC SEQ ID NO: 16_IGHJ1TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5591AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGTAGACACGTACAGTGC TGATGGCGCGAGGGAGGChsIGH_2014_D014_J001_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACGTACAGTGTGGGCATGGACAG SEQ IDNO: 22_IGHJ1TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5592AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGCACTGTACGTACAGTGCTGATGG CGCGAGGGAGGChsIGH_2015_D015_J001_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCGTACAGTGCAAGGGTGAGTCA SEQ ID NO: 04_IGHJ1GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5593GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGATGATCCGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2016_D016_J001_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAGTACAGTGTGCCTCTCTCCCC SEQ ID NO: 11_IGHJ1AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5594GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGCGCCAATAGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2017_D017_J001_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTGTACAGTGGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ1GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5595GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTCAAGCCTGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2018_D018_J001_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTGTACAGTGGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ1GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5596GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGACGTGTGTGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2019_D019_J001_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAGTACAGTGAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ1GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5597GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTCCGTCTAGTACAGTGCTGATGGCGCGAGGGAGG ChsIGH_2020_D020_J001_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGGTACAGTGGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ1CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5598CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGAAGAGCTGGTACAGTGCTGATGGCGCGAGGG AGGChsIGH_2021_D021_J001_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCGTACAGTGGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ1CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5599CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTATCGCTCGTACAGTGCTGATGGCGCGAGGGAGG ChsIGH_2022_D022_J001_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCGTACAGTGGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ1CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5600TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTCAGATGCGTACAGTGCTGATGGCGCGAGGGAGG ChsIGH_2023_D023_J001_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAGTACAGTGAGGCAGCTGACTC SEQ ID NO: 06_IGHJ1CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5601AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGGTGTAGCAGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2024_D024_J001_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTGTACAGTGAGGCAGCTGACCC SEQ ID NO: 13_IGHJ1CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5602AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTGGCAGTTGTACAGTGCTGATGGCGCGAGGGAG GChsIGH_2025_D025_J001_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAGTACAGTGTGAGGTAGCTGGC SEQ ID NO: 19_IGHJ1CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5603CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGCAGTCCAAGTACAGTGCTGATGGCGCGAGGGAG GChsIGH_2026_D026_J001_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGGTACAGTGCAGCTGGCCTCTG SEQ ID NO: 25_IGHJ1TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5604ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGTACGTACGGTACAGTGCTGATGGCGCGAGGGAGGChsIGH_2027_D027_J001_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAGTACAGTGAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ1GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5605CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAGTACAGTGGTCGACATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGGTGAGTCTGCTGTCTGGGGATAGCGGGGAGCCAGGTGTACTGGGCCAGGCAAGAGTACCGAGTACAGTGCTGATGGCGCGAGGGAGGC hsIGH_2028_D001_J002_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTGGATCATCGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ2TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5606CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGACACTCTGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2029_D002_J002_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACGGATCATCGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ2GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5607ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATTCGGAACGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2030_D003_J002_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGGGATCATCGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ2GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5608ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAAAGTAACGGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2031_D004_J002_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAGGATCATCGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ2TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5609CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGTCTCCTAGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2032_D005_J002_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCGGATCATCAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ2TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5610TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAAGAGTGTCGGATCATCCTGATGGCGCGAGGGAGG ChsIGH_2033_D006_J002_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAGGATCATCAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ2CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5611AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGTTCCGAAGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2034_D007_J002_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTGGATCATCAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ2CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5612AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGACGTTACTTGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2035_D008_J002_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACGGATCATCAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ2CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5613AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATAGGAGACGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2036_D009_J002_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACGGATCATCAGCCCCCTGTACA SEQ ID NO: 21_IGHJ2GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5614ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGTGTCTACGGATCATCCTGATGGCGC GAGGGAGGChsIGH_2037_D010_J002_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACGGATCATCGTGGGCACGGACA SEQ ID NO: 03_IGHJ2CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5615GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATGCTACACGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2038_D011_J002_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAGGATCATCTGGGCACGGACAC SEQ ID NO: 09_IGHJ2TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5616AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAAACTGCCAGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2039_D012_J002_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGGGATCATCCGATATTTTGACT SEQ ID NO: 10_IGHJ2GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5617GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATTGGACTGGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2040_D013_J002_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACGGATCATCTGGACGCGGACAC SEQ ID NO: 16_IGHJ2TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5618AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGTAGACACGGATCATCC TGATGGCGCGAGGGAGGChsIGH_2041_D014_J002_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACGGATCATCTGGGCATGGACAG SEQ ID NO: 22_IGHJ2TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5619AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGACACTGTACGGATCATCCTGATGG CGCGAGGGAGGChsIGH_2042_D015_J002_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCGGATCATCCAAGGGTGAGTCA SEQ ID NO: 04_IGHJ2GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5620GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGATGATCCGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2043_D016_J002_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAGGATCATCTGCCTCTCTCCCC SEQ ID NO: 11_IGHJ2AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5621GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGACGCCAATAGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2044_D017_J002_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTGGATCATCGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ2GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5622GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATCAAGCCTGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2045_D018_J002_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTGGATCATCGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ2GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5623GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAACGTGTGTGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2046_D019_J002_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAGGATCATCAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ2GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5624GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATCCGTCTAGGATCATCCTGATGGCGCGAGGGAGG ChsIGH_2047_D020_J002_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGGGATCATCGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ2CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5625CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAAAGAGCTGGGATCATCCTGATGGCGCGAGGG AGGChsIGH_2048_D021_J002_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCGGATCATCGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ2CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5626CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATATCGCTCGGATCATCCTGATGGCGCGAGGGAGG ChsIGH_2049_D022_J002_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCGGATCATCGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ2CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5627TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATCAGATGCGGATCATCCTGATGGCGCGAGGGAGG ChsIGH_2050_D023_J002_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAGGATCATCAGGCAGCTGACTC SEQ ID NO: 06_IGHJ2CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5628AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAGTGTAGCAGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2051_D024_J002_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTGGATCATCAGGCAGCTGACCC SEQ ID NO: 13_IGHJ2CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5629AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATGGCAGTTGGATCATCCTGATGGCGCGAGGGAG GChsIGH_2052_D025_J002_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAGGATCATCTGAGGTAGCTGGC SEQ ID NO: 19_IGHJ2CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5630CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGACAGTCCAAGGATCATCCTGATGGCGCGAGGGAG GChsIGH_2053_D026_J002_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGGGATCATCCAGCTGGCCTCTG SEQ ID NO: 25_IGHJ2TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5631ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGATACGTACGGGATCATCCTGATGGCGCGAGGGAGGChsIGH_2054_D027_J002_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAGGATCATCAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ2GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5632CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAGGATCATCGTCGACTGCTGGGGGCCCCTGGACCCGACCCGCCCTGGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTGGCTGAGCTGAAGTACCGAGGATCATCCTGATGGCGCGAGGGAGGC hsIGH_2055_D001_J003_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTTATTGGCGGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ3TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5633CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGACACTCTTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2056_D002_J003_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACTATTGGCGGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ3GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5634ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATTCGGAACTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2057_D003_J003_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGTATTGGCGGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ3GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5635ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAAAGTAACGTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2058_D004_J003_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTATATTGGCGGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ3TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5636CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGTCTCCTATATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2059_D005_J003_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCTATTGGCGAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ3TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5637TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAAGAGTGTCTATTGGCGCTGATGGCGCGAGGGAGG ChsIGH_2060_D006_J003_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAATATTGGCGAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ3CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5638AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGTTCCGAATATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2061_D007_J003_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTTATTGGCGAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ3CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5639AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCACGTTACTTTATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2062_D008_J003_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACTATTGGCGAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ3CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5640AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATAGGAGACTATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2063_D009_J003_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACTATTGGCGAGCCCCCTGTACA SEQ ID NO: 21_IGHJ3GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5641ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGTGTCTACTATTGGCGCTGATGGCGC GAGGGAGGChsIGH_2064_D010_J003_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACTATTGGCGGTGGGCACGGACA SEQ ID NO: 03_IGHJ3CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5642GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATGCTACACTATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2065_D011_J003_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCATATTGGCGTGGGCACGGACAC SEQ ID NO: 09_IGHJ3TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5643AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAAACTGCCATATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2066_D012_J003_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGTATTGGCGCGATATTTTGACT SEQ ID NO: 10_IGHJ3GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5644GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATTGGACTGTATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2067_D013_J003_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACTATTGGCGTGGACGCGGACAC SEQ ID NO: 16_IGHJ3TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5645AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGTAGACACTATTGGCGC TGATGGCGCGAGGGAGGChsIGH_2068_D014_J003_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACTATTGGCGTGGGCATGGACAG SEQ ID NO: 22_IGHJ3TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5646AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCACACTGTACTATTGGCGCTGATGG CGCGAGGGAGGChsIGH_2069_D015_J003_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCTATTGGCGCAAGGGTGAGTCA SEQ ID NO: 04_IGHJ3GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5647GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGATGATCCTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2070_D016_J003_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATATATTGGCGTGCCTCTCTCCCC SEQ ID NO: 11_IGHJ3AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5648GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCACGCCAATATATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2071_D017_J003_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTTATTGGCGGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ3GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5649GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATCAAGCCTTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2072_D018_J003_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTTATTGGCGGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ3GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5650GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAACGTGTGTTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2073_D019_J003_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTATATTGGCGAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ3GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5651GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATCCGTCTATATTGGCGCTGATGGCGCGAGGGAGG ChsIGH_2074_D020_J003_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGTATTGGCGGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ3CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5652CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAAAGAGCTGTATTGGCGCTGATGGCGCGAGGG AGGChsIGH_2075_D021_J003_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCTATTGGCGGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ3CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5653CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATATCGCTCTATTGGCGCTGATGGCGCGAGGGAGG ChsIGH_2076_D022_J003_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCTATTGGCGGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ3CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5654TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATCAGATGCTATTGGCGCTGATGGCGCGAGGGAGG ChsIGH_2077_D023_J003_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCATATTGGCGAGGCAGCTGACTC SEQ ID NO: 06_IGHJ3CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5655AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAGTGTAGCATATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2078_D024_J003_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTTATTGGCGAGGCAGCTGACCC SEQ ID NO: 13_IGHJ3CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5656AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATGGCAGTTTATTGGCGCTGATGGCGCGAGGGAG GChsIGH_2079_D025_J003_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAATATTGGCGTGAGGTAGCTGGC SEQ ID NO: 19_IGHJ3CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5657CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCACAGTCCAATATTGGCGCTGATGGCGCGAGGGAG GChsIGH_2080_D026_J003_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGTATTGGCGCAGCTGGCCTCTG SEQ ID NO: 25_IGHJ3TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5658ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGTATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCATACGTACGTATTGGCGCTGATGGCGCGAGGGAGGChsIGH_2081_D027_J003_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGATATTGGCGAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ3GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5659CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGATATTGGCGGTCGACGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGGTGAGTCCCACTGCAGCCCCCTCCCAGTCTTCTCTGTCCAGGCACCAGGCCAAGTACCGATATTGGCGCTGATGGCGCGAGGGAGGC hsIGH_2082_D001_J004_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTAGGCTTGAGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ4TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5660CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGACACTCTAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2083_D002_J004_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACAGGCTTGAGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ4GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5661ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTTCGGAACAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2084_D003_J004_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGAGGCTTGAGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ4GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5662ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGAAGTAACGAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2085_D004_J004_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAAGGCTTGAGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ4TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5663CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGTCTCCTAAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2086_D005_J004_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCAGGCTTGAAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ4TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5664TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGAGAGTGTCAGGCTTGACTGATGGCGCGAGGGAGG ChsIGH_2087_D006_J004_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAAGGCTTGAAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ4CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5665AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGTTCCGAAAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2088_D007_J004_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTAGGCTTGAAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ4CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5666AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGCGTTACTTAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2089_D008_J004_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACAGGCTTGAAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ4CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5667AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTAGGAGACAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2090_D009_J004_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACAGGCTTGAAGCCCCCTGTACA SEQ ID NO: 21_IGHJ4GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5668ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGTGTCTACAGGCTTGACTGATGGCGC GAGGGAGGChsIGH_2091_D010_J004_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACAGGCTTGAGTGGGCACGGACA SEQ ID NO: 03_IGHJ4CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5669GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTGCTACACAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2092_D011_J004_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAAGGCTTGATGGGCACGGACAC SEQ ID NO: 09_IGHJ4TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5670AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGAACTGCCAAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2093_D012_J004_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGAGGCTTGACGATATTTTGACT SEQ ID NO: 10_IGHJ4GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5671GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTTGGACTGAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2094_D013_J004_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACAGGCTTGATGGACGCGGACAC SEQ ID NO: 16_IGHJ4TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5672AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGTAGACACAGGCTTGAC TGATGGCGCGAGGGAGGChsIGH_2095_D014_J004_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACAGGCTTGATGGGCATGGACAG SEQ ID NO: 22_IGHJ4TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5673AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGCACTGTACAGGCTTGACTGATGG CGCGAGGGAGGChsIGH_2096_D015_J004_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCAGGCTTGACAAGGGTGAGTCA SEQ ID NO: 04_IGHJ4GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5674GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGATGATCCAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2097_D016_J004_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAAGGCTTGATGCCTCTCTCCCC SEQ ID NO: 11_IGHJ4AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5675GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGCGCCAATAAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2098_D017_J004_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTAGGCTTGAGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ4GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5676GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTCAAGCCTAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2099_D018_J004_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTAGGCTTGAGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ4GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5677GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGACGTGTGTAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2100_D019_J004_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAAGGCTTGAAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ4GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5678GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTCCGTCTAAGGCTTGACTGATGGCGCGAGGGAGG ChsIGH_2101_D020_J004_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGAGGCTTGAGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ4CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5679CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGAAGAGCTGAGGCTTGACTGATGGCGCGAGGG AGGChsIGH_2102_D021_J004_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCAGGCTTGAGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ4CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5680CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTATCGCTCAGGCTTGACTGATGGCGCGAGGGAGG ChsIGH_2103_D022_J004_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCAGGCTTGAGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ4CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5681TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTCAGATGCAGGCTTGACTGATGGCGCGAGGGAGG ChsIGH_2104_D023_J004_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAAGGCTTGAAGGCAGCTGACTC SEQ ID NO: 06_IGHJ4CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5682AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGGTGTAGCAAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2105_D024_J004_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTAGGCTTGAAGGCAGCTGACCC SEQ ID NO: 13_IGHJ4CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5683AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTGGCAGTTAGGCTTGACTGATGGCGCGAGGGAG GChsIGH_2106_D025_J004_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAAGGCTTGATGAGGTAGCTGGC SEQ ID NO: 19_IGHJ4CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5684CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGCAGTCCAAAGGCTTGACTGATGGCGCGAGGGAG GChsIGH_2107_D026_J004_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGAGGCTTGACAGCTGGCCTCTG SEQ ID NO: 25_IGHJ4TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5685ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGTACGTACGAGGCTTGACTGATGGCGCGAGGGAGGChsIGH_2108_D027_J004_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAAGGCTTGAAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ4GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5686CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAAGGCTTGAGTCGACAAGTGCTTGGAGCACTGGGGCCAGGGCAGCCCGGCCACCGTCTCCCTGGGAACGTCACCCCTCCCTGCCTGGGTCTCAGCCCGGGGGTCTGTGTGGCTGGAGTACCGAAGGCTTGACTGATGGCGCGAGGGAGGC hsIGH_2109_D001_J005_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTACACACGTGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ5TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5687CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGACACTCTACACACGTCTGATGGCGCGAGGGAGGChsIGH_2110_D002_J005_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACACACACGTGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ5GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5688ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTTCGGAACACACACGTCTGATGGCGCGAGGGAGGChsIGH_2111_D003_J005_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGACACACGTGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ5GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5689ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGAAGTAACGACACACGTCTGATGGCGCGAGGGAGGChsIGH_2112_D004_J005_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAACACACGTGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ5TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5690CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGTCTCCTAACACACGTCTGATGGCGCGAGGGAGGChsIGH_2113_D005_J005_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCACACACGTAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ5TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5691TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGAGAGTGTCACACACGTCTGATGGCGCGAGGGAGG ChsIGH_2114_D006_J005_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAACACACGTAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ5CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5692AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGTTCCGAAACACACGTCTGATGG CGCGAGGGAGGChsIGH_2115_D007_J005_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTACACACGTAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ5CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5693AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGCGTTACTTACACACGTCTGATGG CGCGAGGGAGGChsIGH_2116_D008_J005_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACACACACGTAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ5CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5694AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTAGGAGACACACACGTCTGATGG CGCGAGGGAGGChsIGH_2117_D009_J005_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACACACACGTAGCCCCCTGTACA SEQ ID NO: 21_IGHJ5GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5695ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGTGTCTACACACACGTCTGATGGCGC GAGGGAGGChsIGH_2118_D010_J005_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACACACACGTGTGGGCACGGACA SEQ ID NO: 03_IGHJ5CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5696GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTGCTACACACACACGTCTGATGG CGCGAGGGAGGChsIGH_2119_D011_J005_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAACACACGTTGGGCACGGACAC SEQ ID NO: 09_IGHJ5TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5697AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGAACTGCCAACACACGTCTGATGG CGCGAGGGAGGChsIGH_2120_D012_J005_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGACACACGTCGATATTTTGACT SEQ ID NO: 10_IGHJ5GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5698GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTTGGACTGACACACGTCTGATGG CGCGAGGGAGGChsIGH_2121_D013_J005_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACACACACGTTGGACGCGGACAC SEQ ID NO: 16_IGHJ5TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5699AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGTAGACACACACACGTC TGATGGCGCGAGGGAGGChsIGH_2122_D014_J005_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACACACACGTTGGGCATGGACAG SEQ ID NO: 22_IGHJ5TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5700AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGCACTGTACACACACGTCTGATGG CGCGAGGGAGGChsIGH_2123_D015_J005_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCACACACGTCAAGGGTGAGTCA SEQ ID NO: 04_IGHJ5GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5701GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGATGATCCACACACGTCTGATGGCGCGAGGGAGGChsIGH_2124_D016_J005_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAACACACGTTGCCTCTCTCCCC SEQ ID NO: 11_IGHJ5AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5702GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGCGCCAATAACACACGTCTGATGGCGCGAGGGAGGChsIGH_2125_D017_J005_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTACACACGTGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ5GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5703GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTCAAGCCTACACACGTCTGATGGCGCGAGGGAGGChsIGH_2126_D018_J005_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTACACACGTGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ5GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5704GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGACGTGTGTACACACGTCTGATGGCGCGAGGGAGGChsIGH_2127_D019_J005_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAACACACGTAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ5GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5705GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTCCGTCTAACACACGTCTGATGGCGCGAGGGAGG ChsIGH_2128_D020_J005_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGACACACGTGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ5CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5706CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGAAGAGCTGACACACGTCTGATGGCGCGAGGG AGGChsIGH_2129_D021_J005_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCACACACGTGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ5CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5707CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTATCGCTCACACACGTCTGATGGCGCGAGGGAGG ChsIGH_2130_D022_J005_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCACACACGTGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ5CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5708TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTCAGATGCACACACGTCTGATGGCGCGAGGGAGG ChsIGH_2131_D023_J005_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAACACACGTAGGCAGCTGACTC SEQ ID NO: 06_IGHJ5CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5709AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGGTGTAGCAACACACGTCTGATGGCGCGAGGGAGGChsIGH_2132_D024_J005_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTACACACGTAGGCAGCTGACCC SEQ ID NO: 13_IGHJ5CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5710AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTGGCAGTTACACACGTCTGATGGCGCGAGGGAG GChsIGH_2133_D025_J005_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAACACACGTTGAGGTAGCTGGC SEQ ID NO: 19_IGHJ5CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5711CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGCAGTCCAAACACACGTCTGATGGCGCGAGGGAG GChsIGH_2134_D026_J005_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGACACACGTCAGCTGGCCTCTG SEQ ID NO: 25_IGHJ5TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5712ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGTACGTACGACACACGTCTGATGGCGCGAGGGAGGChsIGH_2135_D027_J005_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAACACACGTAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ5GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5713CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAACACACGTGTCGACCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGGTAAGATGGCTTTCCTTCTGCCTCCTTTCTCTGGGCCCAGCGTCCTCTGTCCTGGAGTACCGAACACACGTCTGATGGCGCGAGGGAGGC hsIGH_2136_D001_J006_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTTAGACGGAGCCCCGGTCTCTG SEQ ID NO: 01_IGHJ6TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5714CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGACACTCTTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2137_D002_J006_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACTAGACGGAGGCCTCGGTCTCT SEQ ID NO: 07_IGHJ6GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5715ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTTCGGAACTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2138_D003_J006_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGTAGACGGAGGCCTCGGTCTCT SEQ ID NO: 14_IGHJ6GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5716ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGAAGTAACGTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2139_D004_J006_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTATAGACGGAGTCTCTGTGGGTG SEQ ID NO: 20_IGHJ6TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5717CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGTCTCCTATAGACGGACTGATGGCGCGAGGGAGGChsIGH_2140_D005_J006_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCTAGACGGAAGGCCTCAGGCTC SEQ ID NO: 26_IGHJ6TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5718TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGAGAGTGTCTAGACGGACTGATGGCGCGAGGGAGG ChsIGH_2141_D006_J006_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAATAGACGGAAAAGGAGGAGCCC SEQ ID NO: 02_IGHJ6CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5719AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGTTCCGAATAGACGGACTGATGG CGCGAGGGAGGChsIGH_2142_D007_J006_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTTAGACGGAAAAGGAGGAGCCC SEQ ID NO: 08_IGHJ6CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5720AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGCGTTACTTTAGACGGACTGATGG CGCGAGGGAGGChsIGH_2143_D008_J006_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACTAGACGGAAAAGGAGGAGCCC SEQ ID NO: 15_IGHJ6CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5721AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTAGGAGACTAGACGGACTGATGG CGCGAGGGAGGChsIGH_2144_D009_J006_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACTAGACGGAAGCCCCCTGTACA SEQ ID NO: 21_IGHJ6GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5722ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGTGTCTACTAGACGGACTGATGGCGC GAGGGAGGChsIGH_2145_D010_J006_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACTAGACGGAGTGGGCACGGACA SEQ ID NO: 03_IGHJ6CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5723GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTGCTACACTAGACGGACTGATGG CGCGAGGGAGGChsIGH_2146_D011_J006_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCATAGACGGATGGGCACGGACAC SEQ ID NO: 09_IGHJ6TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5724AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGAACTGCCATAGACGGACTGATGG CGCGAGGGAGGChsIGH_2147_D012_J006_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGTAGACGGACGATATTTTGACT SEQ ID NO: 10_IGHJ6GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5725GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTTGGACTGTAGACGGACTGATGG CGCGAGGGAGGChsIGH_2148_D013_J006_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACTAGACGGATGGACGCGGACAC SEQ ID NO: 16_IGHJ6TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5726AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGTAGACACTAGACGGAC TGATGGCGCGAGGGAGGChsIGH_2149_D014_J006_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACTAGACGGATGGGCATGGACAG SEQ ID NO: 22_IGHJ6TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5727AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGCACTGTACTAGACGGACTGATGG CGCGAGGGAGGChsIGH_2150_D015_J006_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCTAGACGGACAAGGGTGAGTCA SEQ ID NO: 04_IGHJ6GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5728GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGATGATCCTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2151_D016_J006_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATATAGACGGATGCCTCTCTCCCC SEQ ID NO: 11_IGHJ6AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5729GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGCGCCAATATAGACGGACTGATGGCGCGAGGGAGGChsIGH_2152_D017_J006_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTTAGACGGAGGAGGGTGAGTCA SEQ ID NO: 17_IGHJ6GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5730GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTCAAGCCTTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2153_D018_J006_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTTAGACGGAGGAGGGTGAGTCA SEQ ID NO: 23_IGHJ6GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5731GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGACGTGTGTTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2154_D019_J006_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTATAGACGGAAGAGGCCTCTCCA SEQ ID NO: 05_IGHJ6GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5732GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTCCGTCTATAGACGGACTGATGGCGCGAGGGAGG ChsIGH_2155_D020_J006_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGTAGACGGAGCAGAGGCCTCTC SEQ ID NO: 12_IGHJ6CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5733CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGAAGAGCTGTAGACGGACTGATGGCGCGAGGG AGGChsIGH_2156_D021_J006_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCTAGACGGAGCAGAGGCCTCTC SEQ ID NO: 18_IGHJ6CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5734CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTATCGCTCTAGACGGACTGATGGCGCGAGGGAGG ChsIGH_2157_D022_J006_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCTAGACGGAGCAGAGGCCTCTC SEQ ID NO: 24_IGHJ6CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5735TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTCAGATGCTAGACGGACTGATGGCGCGAGGGAGG ChsIGH_2158_D023_J006_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCATAGACGGAAGGCAGCTGACTC SEQ ID NO: 06_IGHJ6CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5736AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGGTGTAGCATAGACGGACTGATGGCGCGAGGGAGGChsIGH_2159_D024_J006_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTTAGACGGAAGGCAGCTGACCC SEQ ID NO: 13_IGHJ6CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5737AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTGGCAGTTTAGACGGACTGATGGCGCGAGGGAG GChsIGH_2160_D025_J006_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAATAGACGGATGAGGTAGCTGGC SEQ ID NO: 19_IGHJ6CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5738CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGCAGTCCAATAGACGGACTGATGGCGCGAGGGAG GChsIGH_2161_D026_J006_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGTAGACGGACAGCTGGCCTCTG SEQ ID NO: 25_IGHJ6TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5739ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGTAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGTACGTACGTAGACGGACTGATGGCGCGAGGGAGGChsIGH_2162_D027_J006_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGATAGACGGAAGGGTTGAGGGCT SEQ ID NO: 27_IGHJ6GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5740CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGATAGACGGAGTCGACAGTTGGACTTCCCAGGCCGACAGTGGTCTGGCTTCTGAGGGGTCAGGCCAGAATGTGGGGTACGTGGGAGGCCAGCAGAGGGTTCCATGAGAAGGGCAGGAGTACCGATAGACGGACTGATGGCGCGAGGGAGGC hsIGH_2163_D001_J007_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTCAGCTCTTGCCCCGGTCTCTG SEQ ID NO: 01_IGHJp1TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5741CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGACACTCTCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2164_D002_J007_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACCAGCTCTTGGCCTCGGTCTCT SEQ ID NO: 07_IGHJp1GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5742ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTTCGGAACCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2165_D003_J007_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGCAGCTCTTGGCCTCGGTCTCT SEQ ID NO: 14_IGHJp1GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5743ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGAAGTAACGCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2166_D004_J007_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTACAGCTCTTGTCTCTGTGGGTG SEQ ID NO: 20_IGHJp1TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5744CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCT GAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGTCTCCTACAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2167_D005_J007_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCCAGCTCTTAGGCCTCAGGCTC SEQ ID NO: 26_IGHJp1TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5745TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGAGAGTGTCCAGCTCTTCTGATGGCGCGAGGGAGG ChsIGH_2168_D006_J007_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAACAGCTCTTAAAGGAGGAGCCC SEQ ID NO: 02_IGHJp1CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5746AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGTTCCGAACAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2169_D007_J007_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTCAGCTCTTAAAGGAGGAGCCC SEQ ID NO: 08_IGHJp1CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5747AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGCGTTACTTCAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2170_D008_J007_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACCAGCTCTTAAAGGAGGAGCCC SEQ ID NO: 15_IGHJp1CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5748AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTAGGAGACCAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2171_D009_J007_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACCAGCTCTTAGCCCCCTGTACA SEQ ID NO: 21_IGHJp1GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5749ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGTGTCTACCAGCTCTTCTGATGGCGC GAGGGAGGChsIGH_2172_D010_J007_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACCAGCTCTTGTGGGCACGGACA SEQ ID NO: 03_IGHJp1CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5750GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTGCTACACCAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2173_D011_J007_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCACAGCTCTTTGGGCACGGACAC SEQ ID NO: 09_IGHJp1TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5751AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGAACTGCCACAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2174_D012_J007_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGCAGCTCTTCGATATTTTGACT SEQ ID NO: 10_IGHJp1GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5752GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTTGGACTGCAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2175_D013_J007_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACCAGCTCTTTGGACGCGGACAC SEQ ID NO: 16_IGHJp1TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5753AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGTAGACACCAGCTCTTC TGATGGCGCGAGGGAGGChsIGH_2176_D014_J007_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACCAGCTCTTTGGGCATGGACAG SEQ ID NO: 22_IGHJp1TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5754AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGCACTGTACCAGCTCTTCTGATGG CGCGAGGGAGGChsIGH_2177_D015_J007_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCCAGCTCTTCAAGGGTGAGTCA SEQ ID NO: 04_IGHJp1GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5755GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGATGATCCCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2178_D016_J007_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATACAGCTCTTTGCCTCTCTCCCC SEQ ID NO: 11_IGHJp1AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5756GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGCGCCAATACAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2179_D017_J007_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTCAGCTCTTGGAGGGTGAGTCA SEQ ID NO: 17_IGHJp1GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5757GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTCAAGCCTCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2180_D018_J007_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTCAGCTCTTGGAGGGTGAGTCA SEQ ID NO: 23_IGHJp1GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5758GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGACGTGTGTCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2181_D019_J007_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTACAGCTCTTAGAGGCCTCTCCA SEQ ID NO: 05_IGHJp1GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5759GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTCCGTCTACAGCTCTTCTGATGGCGCGAGGGAGG ChsIGH_2182_D020_J007_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGCAGCTCTTGCAGAGGCCTCTC SEQ ID NO: 12_IGHJp1CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5760CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGAAGAGCTGCAGCTCTTCTGATGGCGCGAGGG AGGChsIGH_2183_D021_J007_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCCAGCTCTTGCAGAGGCCTCTC SEQ ID NO: 18_IGHJp1CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5761CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTATCGCTCCAGCTCTTCTGATGGCGCGAGGGAGG ChsIGH_2184_D022_J007_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCCAGCTCTTGCAGAGGCCTCTC SEQ ID NO: 24_IGHJp1CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5762TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTCAGATGCCAGCTCTTCTGATGGCGCGAGGGAGG ChsIGH_2185_D023_J007_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCACAGCTCTTAGGCAGCTGACTC SEQ ID NO: 06_IGHJp1CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5763AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGGTGTAGCACAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2186_D024_J007_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTCAGCTCTTAGGCAGCTGACCC SEQ ID NO: 13_IGHJp1CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5764AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTGGCAGTTCAGCTCTTCTGATGGCGCGAGGGAG GChsIGH_2187_D025_J007_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAACAGCTCTTTGAGGTAGCTGGC SEQ ID NO: 19_IGHJp1CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5765CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGCAGTCCAACAGCTCTTCTGATGGCGCGAGGGAG GChsIGH_2188_D026_J007_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGCAGCTCTTCAGCTGGCCTCTG SEQ ID NO: 25_IGHJp1TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5766ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGCAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGTACGTACGCAGCTCTTCTGATGGCGCGAGGGAGGChsIGH_2189_D027_J007_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGACAGCTCTTAGGGTTGAGGGCT SEQ ID NO: 27_IGHJp1GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5767CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGACAGCTCTTGTCGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACAACCTCTCTCCTGCTTTAACTCTGAAGGGTTTTGCTGCATTTCTGAGTACCGACAGCTCTTCTGATGGCGCGAGGGAGGC hsIGH_2190_D001_J008_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTGAGCGATAGCCCCGGTCTCTG SEQ ID NO: 01_IGHJp2TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5768CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGACACTCTGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2191_D002_J008_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACGAGCGATAGGCCTCGGTCTCT SEQ ID NO: 07_IGHJp2GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5769ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTTCGGAACGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2192_D003_J008_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGGAGCGATAGGCCTCGGTCTCT SEQ ID NO: 14_IGHJp2GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5770ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGAAGTAACGGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2193_D004_J008_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAGAGCGATAGTCTCTGTGGGTG SEQ ID NO: 20_IGHJp2TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5771CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGTCTCCTAGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2194_D005_J008_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCGAGCGATAAGGCCTCAGGCTC SEQ ID NO: 26_IGHJp2TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5772TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGAGAGTGTCGAGCGATACTGATGGCGCGAGGGAGG ChsIGH_2195_D006_J008_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAGAGCGATAAAAGGAGGAGCCC SEQ ID NO: 02_IGHJp2CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5773AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGTTCCGAAGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2196_D007_J008_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTGAGCGATAAAAGGAGGAGCCC SEQ ID NO: 08_IGHJp2CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5774AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGCGTTACTTGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2197_D008_J008_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACGAGCGATAAAAGGAGGAGCCC SEQ ID NO: 15_IGHJp2CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5775AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTAGGAGACGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2198_D009_J008_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACGAGCGATAAGCCCCCTGTACA SEQ ID NO: 21_IGHJp2GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5776ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGTGTCTACGAGCGATACTGATGGCGC GAGGGAGGChsIGH_2199_D010_J008_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACGAGCGATAGTGGGCACGGACA SEQ ID NO: 03_IGHJp2CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5777GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTGCTACACGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2200_D011_J008_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAGAGCGATATGGGCACGGACAC SEQ ID NO: 09_IGHJp2TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5778AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGAACTGCCAGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2201_D012_J008_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGGAGCGATACGATATTTTGACT SEQ ID NO: 10_IGHJp2GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5779GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTTGGACTGGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2202_D013_J008_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACGAGCGATATGGACGCGGACAC SEQ ID NO: 16_IGHJp2TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5780AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGTAGACACGAGCGATAC TGATGGCGCGAGGGAGGChsIGH_2203_D014_J008_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACGAGCGATATGGGCATGGACAG SEQ ID NO: 22_IGHJp2TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5781AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGCACTGTACGAGCGATACTGATGG CGCGAGGGAGGChsIGH_2204_D015_J008_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCGAGCGATACAAGGGTGAGTCA SEQ ID NO: 04_IGHJp2GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5782GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGATGATCCGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2205_D016_J008_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAGAGCGATATGCCTCTCTCCCC SEQ ID NO: 11_IGHJp2AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5783GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGCGCCAATAGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2206_D017_J008_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTGAGCGATAGGAGGGTGAGTCA SEQ ID NO: 17_IGHJp2GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5784GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTCAAGCCTGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2207_D018_J008_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTGAGCGATAGGAGGGTGAGTCA SEQ ID NO: 23_IGHJp2GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5785GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGACGTGTGTGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2208_D019_J008_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAGAGCGATAAGAGGCCTCTCCA SEQ ID NO: 05_IGHJp2GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5786GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTCCGTCTAGAGCGATACTGATGGCGCGAGGGAGG ChsIGH_2209_D020_J008_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGGAGCGATAGCAGAGGCCTCTC SEQ ID NO: 12_IGHJp2CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5787CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGAAGAGCTGGAGCGATACTGATGGCGCGAGGG AGGChsIGH_2210_D021_J008_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCGAGCGATAGCAGAGGCCTCTC SEQ ID NO: 18_IGHJp2CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5788CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTATCGCTCGAGCGATACTGATGGCGCGAGGGAGG ChsIGH_2211_D022_J008_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCGAGCGATAGCAGAGGCCTCTC SEQ ID NO: 24_IGHJp2CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5789TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTCAGATGCGAGCGATACTGATGGCGCGAGGGAGG ChsIGH_2212_D023_J008_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAGAGCGATAAGGCAGCTGACTC SEQ ID NO: 06_IGHJp2CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5790AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGGTGTAGCAGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2213_D024_J008_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTGAGCGATAAGGCAGCTGACCC SEQ ID NO: 13_IGHJp2CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5791AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTGGCAGTTGAGCGATACTGATGGCGCGAGGGAG GChsIGH_2214_D025_J008_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAGAGCGATATGAGGTAGCTGGC SEQ ID NO: 19_IGHJp2CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5792CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGCAGTCCAAGAGCGATACTGATGGCGCGAGGGAG GChsIGH_2215_D026_J008_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGGAGCGATACAGCTGGCCTCTG SEQ ID NO: 25_IGHJp2TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5793ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGTACGTACGGAGCGATACTGATGGCGCGAGGGAGGChsIGH_2216_D027_J008_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAGAGCGATAAGGGTTGAGGGCT SEQ ID NO: 27_IGHJp2GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5794CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAGAGCGATAGTCGACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGTGAGTCCTCACCACCCCCTCTCTGAGTCCACTTAGGGAGACTCAGCTTGCCAGAGTACCGAGAGCGATACTGATGGCGCGAGGGAGGC hsIGH_2217_D001_J009_IGHD1-GCCTTGCCAGCCCGCTCAGGACACTCTGCATCTGAGCCCCGGTCTCTG SEQ ID NO: 01_IGHJp3TGGGTGTTCCGCTAACTGGGGCTCCCAGTGCTCACCCCACAACTAAAG 5795CGAGCCCCAGCCTCCAGAGCCCCCGAAGGAGATGCCGCCCACAAGCCCAGCCCCCATCCAGGAGGCCCCAGAGCTCAGGGCGCCGGGGCAGATTCTGAACAGCCCCGAGTCACGGTGGGTACAACTGGAGACACTCTGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGACACTCTGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2218_D002_J009_IGHD1-GCCTTGCCAGCCCGCTCAGTTCGGAACGCATCTGAGGCCTCGGTCTCT SEQ ID NO: 07_IGHJp3GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5796ACGAGCCACAGCCTCAGAGCCCCTGAAGGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCACAGGGCGCCCCGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGATTCGGAACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTTCGGAACGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2219_D003_J009_IGHD1-GCCTTGCCAGCCCGCTCAGAAGTAACGGCATCTGAGGCCTCGGTCTCT SEQ ID NO: 14_IGHJp3GTGGGTGTTCCGCTAGCTGGGGCTCACAGTGCTCACCCCACACCTAAA 5797ATGAGCCACAGCCTCCGGAGCCCCCGCAGAGACCCCGCCCACAAGCCCAGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCCCGTCGGATTCCGAACAGCCCCGAGTCACAGCGGGTATAACCGGAAAGTAACGGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCAAGTAACGGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2220_D004_J009_IGHD1-GCCTTGCCAGCCCGCTCAGGTCTCCTAGCATCTGAGTCTCTGTGGGTG SEQ ID NO: 20_IGHJp3TTCCGCTAGCTGGGGCCCCCAGTGCTCACCCCACACCTAAAGCGAGCC 5798CCAGCCTCCAGAGCCCCCTAAGCATTCCCCGCCCAGCAGCCCAGCCCCTGCCCCCACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGTCGGATTCTGAACAGCCCCGAGTCACAGTGGGTATAACTGGAGTCTCCTAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGTCTCCTAGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2221_D005_J009_IGHD1-GCCTTGCCAGCCCGCTCAGAGAGTGTCGCATCTGAAGGCCTCAGGCTC SEQ ID NO: 26_IGHJp3TGTGGGTGCCGCTAGCTGGGGCTGCCAGTCCTCACCCCACACCTAAGG 5799TGAGCCACAGCCGCCAGAGCCTCCACAGGAGACCCCACCCAGCAGCCCAGCCCCTACCCAGGAGGCCCCAGAGCTCAGGGCGCCTGGGTGGATTCTGAACAGCCCCGAGTCACGGTGGGTATAGTGGGAGCTAGAGTGTCGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCAGAGTGTCGCATCTGACTGATGGCGCGAGGGAGG ChsIGH_2222_D006_J009_IGHD2-GCCTTGCCAGCCCGCTCAGGTTCCGAAGCATCTGAAAAGGAGGAGCCC SEQ ID NO: 02_IGHJp3CCTGTACAGCACTGGGCTCAGAGTCCTCTCCCACACACCCTGAGTTTC 5800AGACAAAAACCCCCTGGAAATCATAGTATCAGCAGGAGAACTAGCCAGAGACAGCAAGAGGGGACTCAGTGACTCCCGCGGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTAGTACCAGCTGCTGTTCCGAAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGTTCCGAAGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2223_D007_J009_IGHD2-GCCTTGCCAGCCCGCTCAGCGTTACTTGCATCTGAAAAGGAGGAGCCC SEQ ID NO: 08_IGHJp3CTTGTTCAGCACTGGGCTCAGAGTCCTCTCCAAGACACCCAGAGTTTC 5801AGACAAAAACCCCCTGGAATGCACAGTCTCAGCAGGAGAGCCAGCCAGAGCCAGCAAGATGGGGCTCAGTGACACCCGCAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTACTAATGGTGTATGCTCGTTACTTGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCCGTTACTTGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2224_D008_J009_IGHD2-GCCTTGCCAGCCCGCTCAGTAGGAGACGCATCTGAAAAGGAGGAGCCC SEQ ID NO: 15_IGHJp3CCTATACAGCACTGGGCTCAGAGTCCTCTCTGAGACACCCTGAGTTTC 5802AGACAACAACCCGCTGGAATGCACAGTCTCAGCAGGAGAACAGACCAAAGCCAGCAAAAGGGACCTCGGTGACACCAGTAGGGACAGGAGGATTTTGTGGGGGCTCGTGTCACTGTGAGGATATTGTAGTGGTGGTAGCTGCTTAGGAGACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTAGGAGACGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2225_D009_J009_IGHD2-GCCTTGCCAGCCCGCTCAGGTGTCTACGCATCTGAAGCCCCCTGTACA SEQ ID NO: 21_IGHJp3GCACTGGGCTCAGAGTCCTCTCTGAGACAGGCTCAGTTTCAGACAACA 5803ACCCGCTGGAATGCACAGTCTCAGCAGGAGAGCCAGGCCAGAGCCAGCAAGAGGAGACTCGGTGACACCAGTCTCCTGTAGGGACAGGAGGATTTTGTGGGGGTTCGTGTCACTGTGAGCATATTGTGGTGGTGACTGCTGTGTCTACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGTGTCTACGCATCTGACTGATGGCGC GAGGGAGGChsIGH_2226_D010_J009_IGHD3-GCCTTGCCAGCCCGCTCAGTGCTACACGCATCTGAGTGGGCACGGACA SEQ ID NO: 03_IGHJp3CTGTCCACCTAAGCCAGGGGCAGACCCGAGTGTCCCCGCAGTAGACCT 5804GAGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTACCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCTGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACGATTTTTGGAGTGGTTATTTGCTACACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTGCTACACGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2227_D011_J009_IGHD3-GCCTTGCCAGCCCGCTCAGAACTGCCAGCATCTGATGGGCACGGACAC SEQ ID NO: 09_IGHJp3TATCCACATAAGCGAGGGATAGACCCGAGTGTCCCCACAGCAGACCTG 5805AGAGCGCTGGGCCCACAGCCTCCCCTCAGAGCCCTGCTGCCTCCTCCGGTCAGCCCTGGACATCCCAGGTTTCCCCAGGCCTGCCGGTAGGTTTAGAATGAGGTCTGTGTCACTGTGGTATTACGATATTTTGACTGGTTATTAACTGCCAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCAACTGCCAGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2228_D012_J009_IGHD3-GCCTTGCCAGCCCGCTCAGTTGGACTGGCATCTGACGATATTTTGACT SEQ ID NO: 10_IGHJp3GGTTATTATAACCACAGTGTCACAGAGTCCATCAAAAACCCATGCCTG 5806GAAGCTTCCCGCCACAGCCCTCCCCATGGGGCCCTGCTGCCTCCTCAGGTCAGCCCCGGACATCCCGGGTTTCCCCAGGCTGGGCGGTAGGTTTGGGGTGAGGTCTGTGTCACTGTGGTATTACTATGGTTCGGGGAGTTATTTTGGACTGGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTTGGACTGGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2229_D013_J009_IGHD3-GCCTTGCCAGCCCGCTCAGGTAGACACGCATCTGATGGACGCGGACAC SEQ ID NO: 16_IGHJp3TATCCACATAAGCGAGGGACAGACCCGAGTGTTCCTGCAGTAGACCTG 5807AGAGCGCTGGGCCCACAGCCTCCCCTCGGTGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCAGATGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTATGATTACGTTTGGGGGAGTTATCGTTGTAGACACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGTAGACACGCATCTGAC TGATGGCGCGAGGGAGGChsIGH_2230_D014_J009_IGHD3-GCCTTGCCAGCCCGCTCAGCACTGTACGCATCTGATGGGCATGGACAG SEQ ID NO: 22_IGHJp3TGTCCACCTAAGCGAGGGACAGACCCGAGTGTCCCTGCAGTAGACCTG 5808AGAGCGCTGGGCCCACAGCCTCCCCTCGGGGCCCTGCTGCCTCCTCAGGTCAGCCCTGGACATCCCGGGTTTCCCCAGGCCTGGCGGTAGGTTTGAAGTGAGGTCTGTGTCACTGTGGTATTACTATGATAGTAGTGGTTATTCACTGTACGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCCACTGTACGCATCTGACTGATGG CGCGAGGGAGGChsIGH_2231_D015_J009_IGHD4-GCCTTGCCAGCCCGCTCAGGATGATCCGCATCTGACAAGGGTGAGTCA SEQ ID NO: 04_IGHJp3GACCCTCCTGCCCTCGATGGCAGGCGGAGAAGATTCAGAAAGGTCTGA 5809GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCTCTGGGCACACTCAGGGGCTTTTTGTGAAGGGTCCTCCTACTGTGTGACTACAGTAGATGATCCGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGATGATCCGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2232_D016_J009_IGHD4-GCCTTGCCAGCCCGCTCAGCGCCAATAGCATCTGATGCCTCTCTCCCC SEQ ID NO: 11_IGHJp3AGTGGACACCCTCTTCCAGGACAGTCCTCAGTGGCATCACAGCGGCCT 5810GAGATCCCCAGGACGCAGCACCGCTGTCAATAGGGGCCCCAAATGCCTGGACCAGGGCCTGCGTGGGAAAGGTCTCTGGCCACACTCGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACAGTACGCCAATAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCCGCCAATAGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2233_D017_J009_IGHD4-GCCTTGCCAGCCCGCTCAGTCAAGCCTGCATCTGAGGAGGGTGAGTCA SEQ ID NO: 17_IGHJp3GACCCACCTGCCCTCGATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5811GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGCGTGGGAAAGGCCGCTGGGCACACTCAGGGGCTTTTTGTGAAGGCCCCTCCTACTGTGTGACTACGGTGTCAAGCCTGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTCAAGCCTGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2234_D018_J009_IGHD4-GCCTTGCCAGCCCGCTCAGACGTGTGTGCATCTGAGGAGGGTGAGTCA SEQ ID NO: 23_IGHJp3GACCCACCTGCCCTCAATGGCAGGCGGGGAAGATTCAGAAAGGCCTGA 5812GATCCCCAGGACGCAGCACCACTGTCAATGGGGGCCCCAGACGCCTGGACCAGGGCCTGTGTGGGAAAGGCCTCTGGCCACACTCAGGGGCTTTTTGTGAAGGGCCCTCCTGCTGTGTGACTACGGTGGTAACGTGTGTGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCACGTGTGTGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2235_D019_J009_IGHD5-GCCTTGCCAGCCCGCTCAGTCCGTCTAGCATCTGAAGAGGCCTCTCCA SEQ ID NO: 05_IGHJp3GGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCATGTCCCCA 5813GTCCTGGGGGGCCCCCTGGCACAGCTGTCTGGACCCTCTCTATTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTCCGTCTAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTCCGTCTAGCATCTGACTGATGGCGCGAGGGAGG ChsIGH_2236_D020_J009_IGHD5-GCCTTGCCAGCCCGCTCAGAAGAGCTGGCATCTGAGCAGAGGCCTCTC SEQ ID NO: 12_IGHJp3CAGGGAGACACTGTGCATGTCTGGTACCTAAGCAGCCCCCCACGTCCC 5814CAGTCCTGGGGGCCCCTGGCTCAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGCGATGTCAGACTGTGGTGGATATAGTGGCTACGAAGAGCTGGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCAAGAGCTGGCATCTGACTGATGGCGCGAGGG AGGChsIGH_2237_D021_J009_IGHD5-GCCTTGCCAGCCCGCTCAGTATCGCTCGCATCTGAGCAGAGGCCTCTC SEQ ID NO: 18_IGHJp3CAGGGGGACACTGTGCATGTCTGGTCCCTGAGCAGCCCCCCACGTCCC 5815CAGTCCTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGTCAGACTGTGGTGGATACAGCTATGTATCGCTCGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTATCGCTCGCATCTGACTGATGGCGCGAGGGAGG ChsIGH_2238_D022_J009_IGHD5-GCCTTGCCAGCCCGCTCAGTCAGATGCGCATCTGAGCAGAGGCCTCTC SEQ ID NO: 24_IGHJp3CAGGGGGACACAGTGCATGTCTGGTCCCTGAGCAGCCCCCAGGCTCTC 5816TAGCACTGGGGGCCCCTGGCACAGCTGTCTGGACCCTCCCTGTTCCCTGGGAAGCTCCTCCTGACAGCCCCGCCTCCAGTTCCAGGTGTGGTTATTGTCAGGGGGTGCCAGGCCGTGGTAGAGATGGCTACATCAGATGCGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTCAGATGCGCATCTGACTGATGGCGCGAGGGAGG ChsIGH_2239_D023_J009_IGHD6-GCCTTGCCAGCCCGCTCAGGTGTAGCAGCATCTGAAGGCAGCTGACTC SEQ ID NO: 06_IGHJp3CTGACTTGGACGCCTATTCCAGACACCAGACAGAGGGGCAGGCCCCCC 5817AGAACCAGGGATGAGGACGCCCCGTCAAGGCCAGAAAAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACAAACCAGGAACGTTTCTGAAGGTGTCTGTGTCACAGTGGAGTATAGCAGCTGTGTAGCAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCGTGTAGCAGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2240_D024_J009_IGHD6-GCCTTGCCAGCCCGCTCAGTGGCAGTTGCATCTGAAGGCAGCTGACCC SEQ ID NO: 13_IGHJp3CTGACTTGGACCCCTATTCCAGACACCAGACAGAGGCGCAGGCCCCCC 5818AGAACCAGGGTTGAGGGACGCCCCGTCAAAGCCAGACAAAACCAAGGGGTGTTGAGCCCAGCAAGGGAAGGCCCCCAAACAGACCAGGAGGTTTCTGAAGGTGTCTGTGTCACAGTGGGGTATAGCAGCAGCTTGGCAGTTGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTGGCAGTTGCATCTGACTGATGGCGCGAGGGAG GChsIGH_2241_D025_J009_IGHD6-GCCTTGCCAGCCCGCTCAGCAGTCCAAGCATCTGATGAGGTAGCTGGC SEQ ID NO: 19_IGHJp3CTCTGTCTCGGACCCCACTCCAGACACCAGACAGAGGGGCAGGCCCCC 5819CAAAACCAGGGTTGAGGGATGATCCGTCAAGGCAGACAAGACCAAGGGGCACTGACCCCAGCAAGGGAAGGCTCCCAAACAGACGAGGAGGTTTCTGAAGCTGTCTGTATCACAGTGGGGTATAGCAGTGGCTCAGTCCAAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCCAGTCCAAGCATCTGACTGATGGCGCGAGGGAG GChsIGH_2242_D026_J009_IGHD6-GCCTTGCCAGCCCGCTCAGTACGTACGGCATCTGACAGCTGGCCTCTG SEQ ID NO: 25_IGHJp3TCTCGGACCCCCATTCCAGACACCAGACAGAGGGACAGGCCCCCCAGA 5820ACCAGTGTTGAGGGACACCCCTGTCCAGGGCAGCCAAGTCCAAGAGGCGCGCTGAGCCCAGCAAGGGAAGGCCCCCAAACAAACCAGGAGGTTTCTGAAGCTGTCTGTGTCACAGTCGGGTATAGCAGCGTACGTACGGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCTACGTACGGCATCTGACTGATGGCGCGAGGGAGGChsIGH_2243_D027_J009_IGHD7-GCCTTGCCAGCCCGCTCAGAGTACCGAGCATCTGAAGGGTTGAGGGCT SEQ ID NO: 27_IGHJp3GGGGTCTCCCACGTGTTTTGGGGCTAACAGCGGAAGGGAGAGCACTGG 5821CAAAGGTGCTGGGGGTCCCCTGAACCCGACCCGCCCTGAGACCGCAGCCACATCAGCCCCCAGCCCCACAGGCCCCCTACCAGCCGCAGGGTTTTTGGCTGAGCTGAGAACCACTGTGCTAACTAGTACCGAGCATCTGAGTCGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGGTAAGAATGGCCACTCTAGGGCCTTTGTTTTCTGCTACTGCCAGTACCGAGCATCTGACTGATGGCGCGAGGGAGGC Bias Control Sequences forhs-IGL hsIGL_0001_V001_J001_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACGACACTCTGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ1_FTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5822TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTAGGAGACGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0002_V002_J001_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACGACACTCTCCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ1_FGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5823CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGTGTCTACGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0003_V003_J001_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGGACACTCTGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ1_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5824TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGTACAGTGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0004_V004_J001_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCGACACTCTGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ1_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5825TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGGATCATCGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0005_V005_J001_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGGACACTCTCCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5826 ORF_IGLJ1_FCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTATTGGCGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0006_V006_J001_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGAGACACTCTGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ1_FTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 5827TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTAGGCTTGAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0007_V007_J001_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTGACACTCTGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ1_FCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 5828CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTACACACGTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0008_V008_J001_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGAGACACTCTATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ1_FCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 5829CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTAGACGGAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0009_V009_J001_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTGACACTCTGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ1_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5830CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTCAGCTCTTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0010_V010_J001_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATAGACACTCTATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ1_FCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 5831CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGAGCGATAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0011_V011_J001_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGAGACACTCTGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ1_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5832CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGCATCTGAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0012_V012_J001_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACGACACTCTGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 5833 ORF_IGLJ1_FCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTGCTACACGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0013_V013_J001_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCAGACACTCTCTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ1_FCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 5834AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTAACTGCCAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0014_V014_J001_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGGACACTCTTTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 5835 FP_IGLJ1_FGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTTGGACTGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0015_V015_J001_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACGACACTCTTTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ1_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5836AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGTAGACACGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0016_V016_J001_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACGACACTCTTTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ1_FACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 5837AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTCACTGTACGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0017_V017_J001_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCGACACTCTTTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ1_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 5838AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGATGATCCGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0018_V018_J001_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATAGACACTCTTTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ1_FGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 5839AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTCGCCAATAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0019_V019_J001_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTGACACTCTTTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ1_FACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 5840AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTCAAGCCTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0020_V020_J001_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTGACACTCTCCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 5841 FP_IGLJ1_FCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTACGTGTGTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0021_V021_J001_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTAGACACTCTTTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ1_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5842AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTCCGTCTAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0022_V022_J001_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGGACACTCTCTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ1_FTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 5843CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTAAGAGCTGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0023_V023_J001_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCGACACTCTTGCTGACTCAGCCC SEQ ID NO: 03_IGLJ1_FCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 5844TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTATCGCTCGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0024_V024_J001_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCGACACTCTCTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ1_FGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 5845AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTCAGATGCGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0025_V025_J001_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCAGACACTCTCTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ1_FGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 5846AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGTGTAGCAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0026_V026_J001_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTGACACTCTTGTGCTGACTCAGC SEQ ID NO: 37_IGLJ1_FCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 5847CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTGGCAGTTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0027_V027_J001_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAAGACACTCTTGTGCTGACTCAGC SEQ ID NO: 39_IGLJ1_FCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 5848CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTCAGTCCAAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0028_V028_J001_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGGACACTCTTGTGCTGACTCAGC SEQ ID NO: 45_IGLJ1_FCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 5849CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTACGTACGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0029_V029_J001_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGAGACACTCTTGACTCAGCCATCT SEQ ID NO: 52_IGLJ1_FTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 5850GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTAGTACCGAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0030_V030_J001_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGGACACTCTAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ1_FAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5851AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTATCCATGGGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0031_V031_J001_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTGACACTCTAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5852 FP_IGLJ1_FAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTGTAGCAGTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0032_V032_J001_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTGACACTCTGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ1_FACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 5853TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTATCTTCGTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0033_V033_J001_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTGACACTCTTGACTCAGCCACCT SEQ ID NO: 49_IGLJ1_FTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 5854GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTTCCACAGTGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0034_V034_J001_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCGACACTCTTGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 5855 FP_IGLJ1_FCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTATGACACCGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0035_V035_J001_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGAGACACTCTCGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 5856 ORF_IGLJ1_FCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGAGACACTCTGTCGACTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTAAGTGGCTCTCAACCTTTCCCAGCCTGTCTCACCCTCTGCTGTCCCTGGAAAATCTGTTTTCTCTCTTCACGAGACACTCTCTGATGGCGCGAGG GAGGChsIGL_0036_V001_J002_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACTTCGGAACGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ2_FTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5857TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTAGGAGACTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0037_V002_J002_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACTTCGGAACCCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ2_FGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5858CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTGTCTACTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0038_V003_J002_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGTTCGGAACGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ2_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5859TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTACAGTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0039_V004_J002_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCTTCGGAACGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ2_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5860TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGGATCATCTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0040_V005_J002_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGTTCGGAACCCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5861 ORF_IGLJ2_FCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTATTGGCGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0041_V006_J002_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGATTCGGAACGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ2_FTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 5862TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAGGCTTGATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0042_V007_J002_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTTTCGGAACGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ2_FCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 5863CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTACACACGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0043_V008_J002_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGATTCGGAACATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ2_FCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 5864CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTAGACGGATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0044_V009_J002_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTTTCGGAACGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ2_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5865CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCAGCTCTTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0045_V010_J002_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATATTCGGAACATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ2_FCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 5866CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGAGCGATATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0046_V011_J002_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGATTCGGAACGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ2_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5867CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGCATCTGATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0047_V012_J002_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACTTCGGAACGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 5868 ORF_IGLJ2_FCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTGCTACACTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0048_V013_J002_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCATTCGGAACCTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ2_FCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 5869AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAACTGCCATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0049_V014_J002_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGTTCGGAACTTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 5870 FP_IGLJ2_FGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTTGGACTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0050_V015_J002_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACTTCGGAACTTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ2_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5871AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTAGACACTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0051_V016_J002_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACTTCGGAACTTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ2_FACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 5872AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCACTGTACTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0052_V017_J002_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCTTCGGAACTTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ2_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 5873AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGATGATCCTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0053_V018_J002_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATATTCGGAACTTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ2_FGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 5874AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCGCCAATATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0054_V019_J002_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTTTCGGAACTTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ2_FACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 5875AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCAAGCCTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0055_V020_J002_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTTTCGGAACCCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 5876 FP_IGLJ2_FCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTACGTGTGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0056_V021_J002_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTATTCGGAACTTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ2_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5877AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCCGTCTATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0057_V022_J002_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGTTCGGAACCTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ2_FTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 5878CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAAGAGCTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0058_V023_J002_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCTTCGGAACTGCTGACTCAGCCC SEQ ID NO: 03_IGLJ2_FCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 5879TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTATCGCTCTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0059_V024_J002_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCTTCGGAACCTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ2_FGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 5880AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCAGATGCTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0060_V025_J002_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCATTCGGAACCTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ2_FGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 5881AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTGTAGCATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0061_V026_J002_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTTTCGGAACTGTGCTGACTCAGC SEQ ID NO: 37_IGLJ2_FCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 5882CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTGGCAGTTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0062_V027_J002_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAATTCGGAACTGTGCTGACTCAGC SEQ ID NO: 39_IGLJ2_FCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 5883CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCAGTCCAATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0063_V028_J002_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGTTCGGAACTGTGCTGACTCAGC SEQ ID NO: 45_IGLJ2_FCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 5884CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTACGTACGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0064_V029_J002_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGATTCGGAACTGACTCAGCCATCT SEQ ID NO: 52_IGLJ2_FTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 5885GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAGTACCGATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0065_V030_J002_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGTTCGGAACAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ2_FAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5886AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATCCATGGTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0066_V031_J002_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTTTCGGAACAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5887 FP_IGLJ2_FAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTAGCAGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0067_V032_J002_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTTTCGGAACGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ2_FACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 5888TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATCTTCGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0068_V033_J002_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTTTCGGAACTGACTCAGCCACCT SEQ ID NO: 49_IGLJ2_FTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 5889GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCCACAGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0069_V034_J002_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCTTCGGAACTGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 5890 FP_IGLJ2_FCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCTTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATGACACCTTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0070_V035_J002_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGATTCGGAACCGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 5891 ORF_IGLJ2_FCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGATTCGGAACGTCGACTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCTTCACGATTCGGAACCTGATGGCGCGAGG GAGGChsIGL_0071_V001_J003_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACAAGTAACGGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ3_FTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5892TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTAGGAGACAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0072_V002_J003_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACAAGTAACGCCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ3_FGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5893CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTGTCTACAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0073_V003_J003_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGAAGTAACGGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ3_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5894TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTACAGTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0074_V004_J003_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCAAGTAACGGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ3_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5895TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGGATCATCAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0075_V005_J003_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGAAGTAACGCCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5896 ORF_IGLJ3_FCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTATTGGCGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0076_V006_J003_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGAAAGTAACGGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ3_FTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 5897TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAGGCTTGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0077_V007_J003_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTAAGTAACGGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ3_FCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 5898CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTACACACGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0078_V008_J003_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGAAAGTAACGATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ3_FCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 5899CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTAGACGGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0079_V009_J003_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTAAGTAACGGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ3_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5900CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCAGCTCTTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0080_V010_J003_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATAAAGTAACGATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ3_FCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 5901CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGAGCGATAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0081_V011_J003_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGAAAGTAACGGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ3_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5902CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGCATCTGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0082_V012_J003_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACAAGTAACGGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 5903 ORF_IGLJ3_FCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTGCTACACAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0083_V013_J003_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCAAAGTAACGCTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ3_FCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 5904AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAACTGCCAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0084_V014_J003_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGAAGTAACGTTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 5905 FP_IGLJ3_FGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTTGGACTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0085_V015_J003_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACAAGTAACGTTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ3_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5906AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTAGACACAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0086_V016_J003_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACAAGTAACGTTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ3_FACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 5907AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCACTGTACAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0087_V017_J003_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCAAGTAACGTTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ3_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 5908AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGATGATCCAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0088_V018_J003_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATAAAGTAACGTTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ3_FGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 5909AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCGCCAATAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0089_V019_J003_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTAAGTAACGTTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ3_FACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 5910AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCAAGCCTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0090_V020_J003_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTAAGTAACGCCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 5911 FP_IGLJ3_FCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTACGTGTGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0091_V021_J003_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTAAAGTAACGTTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ3_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5912AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCCGTCTAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0092_V022_J003_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGAAGTAACGCTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ3_FTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 5913CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAAGAGCTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0093_V023_J003_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCAAGTAACGTGCTGACTCAGCCC SEQ ID NO: 03_IGLJ3_FCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 5914TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTATCGCTCAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0094_V024_J003_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCAAGTAACGCTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ3_FGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 5915AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCAGATGCAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0095_V025_J003_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCAAAGTAACGCTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ3_FGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 5916AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTGTAGCAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0096_V026_J003_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTAAGTAACGTGTGCTGACTCAGC SEQ ID NO: 37_IGLJ3_FCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 5917CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTGGCAGTTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0097_V027_J003_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAAAAGTAACGTGTGCTGACTCAGC SEQ ID NO: 39_IGLJ3_FCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 5918CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCAGTCCAAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0098_V028_J003_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGAAGTAACGTGTGCTGACTCAGC SEQ ID NO: 45_IGLJ3_FCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 5919CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTACGTACGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0099_V029_J003_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGAAAGTAACGTGACTCAGCCATCT SEQ ID NO: 52_IGLJ3_FTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 5920GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTAGTACCGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0100_V030_J003_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGAAGTAACGAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ3_FAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5921AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATCCATGGAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0101_V031_J003_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTAAGTAACGAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5922 FP_IGLJ3_FAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTGTAGCAGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0102_V032_J003_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTAAGTAACGGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ3_FACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 5923TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATCTTCGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0103_V033_J003_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTAAGTAACGTGACTCAGCCACCT SEQ ID NO: 49_IGLJ3_FTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 5924GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTTCCACAGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0104_V034_J003_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCAAGTAACGTGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 5925 FP_IGLJ3_FCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTATGACACCAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0105_V035_J003_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGAAAGTAACGCGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 5926 ORF_IGLJ3_FCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGAAAGTAACGGTCGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTGAGTCTCTTCTCCCCTCTCCTTCCCCGCTCTTGGGACAATTTCTGCTGTTTTTGTTTGTTTCTGTCTTCACGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGL_0106_V001_J004_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACGTCTCCTAGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ4_PTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5927TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTAGGAGACGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0107_V002_J004_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACGTCTCCTACCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ4_PGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5928CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGTGTCTACGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0108_V003_J004_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGGTCTCCTAGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ4_PTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5929TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGTACAGTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0109_V004_J004_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCGTCTCCTAGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ4_PTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5930TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGGATCATCGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0110_V005_J004_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGGTCTCCTACCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5931 ORF_IGLJ4_PCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTATTGGCGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0111_V006_J004_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGAGTCTCCTAGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ4_PTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 5932TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGAGGCTTGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0112_V007_J004_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTGTCTCCTAGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ4_PCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 5933CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGACACACGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0113_V008_J004_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGAGTCTCCTAATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ4_PCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 5934CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTAGACGGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0114_V009_J004_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTGTCTCCTAGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ4_PCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5935CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGCAGCTCTTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0115_V010_J004_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATAGTCTCCTAATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ4_PCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 5936CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGAGCGATAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0116_V011_J004_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGAGTCTCCTAGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ4_PCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5937CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGCATCTGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0117_V012_J004_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACGTCTCCTAGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 5938 ORF_IGLJ4_PCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTGCTACACGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0118_V013_J004_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCAGTCTCCTACTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ4_PCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 5939AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGAACTGCCAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0119_V014_J004_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGGTCTCCTATTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 5940 FP_IGLJ4_PGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTTGGACTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0120_V015_J004_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACGTCTCCTATTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ4_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5941AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGTAGACACGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0121_V016_J004_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACGTCTCCTATTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ4_PACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 5942AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGCACTGTACGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0122_V017_J004_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCGTCTCCTATTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ4_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 5943AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGATGATCCGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0123_V018_J004_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATAGTCTCCTATTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ4_PGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 5944AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGCGCCAATAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0124_V019_J004_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTGTCTCCTATTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ4_PACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 5945AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTCAAGCCTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0125_V020_J004_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTGTCTCCTACCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 5946 FP_IGLJ4_PCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGACGTGTGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0126_V021_J004_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTAGTCTCCTATTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ4_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5947AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTCCGTCTAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0127_V022_J004_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGGTCTCCTACTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ4_PTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 5948CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGAAGAGCTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0128_V023_J004_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCGTCTCCTATGCTGACTCAGCCC SEQ ID NO: 03_IGLJ4_PCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 5949TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTATCGCTCGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0129_V024_J004_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCGTCTCCTACTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ4_PGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 5950AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTCAGATGCGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0130_V025_J004_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCAGTCTCCTACTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ4_PGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 5951AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGTGTAGCAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0131_V026_J004_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTGTCTCCTATGTGCTGACTCAGC SEQ ID NO: 37_IGLJ4_PCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 5952CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTGGCAGTTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0132_V027_J004_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAAGTCTCCTATGTGCTGACTCAGC SEQ ID NO: 39_IGLJ4_PCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 5953CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGCAGTCCAAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0133_V028_J004_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGGTCTCCTATGTGCTGACTCAGC SEQ ID NO: 45_IGLJ4_PCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 5954CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTACGTACGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0134_V029_J004_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGAGTCTCCTATGACTCAGCCATCT SEQ ID NO: 52_IGLJ4_PTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 5955GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGAGTACCGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0135_V030_J004_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGGTCTCCTAAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ4_PAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5956AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGATCCATGGGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0136_V031_J004_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTGTCTCCTAAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5957 FP_IGLJ4_PAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGGTAGCAGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0137_V032_J004_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTGTCTCCTAGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ4_PACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 5958TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGATCTTCGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0138_V033_J004_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTGTCTCCTATGACTCAGCCACCT SEQ ID NO: 49_IGLJ4_PTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 5959GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGTCCACAGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0139_V034_J004_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCGTCTCCTATGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 5960 FP_IGLJ4_PCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGATGACACCGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0140_V035_J004_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGAGTCTCCTACGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 5961 ORF_IGLJ4_PCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGAGTCTCCTAGTCGACTATTTGGTGGAGGAACCCAGCTGATCATTTTAGATGAGTCTCTTCTTCCCTTTCTTTCCCTGCCAAGTTGGTGACAATTTTATTCTGATTTCGATCTTTGCTTCACGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGL_0141_V001_J005_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACAGAGTGTCGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ5_PTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5962TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTAGGAGACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0142_V002_J005_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACAGAGTGTCCCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ5_PGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5963CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGTGTCTACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0143_V003_J005_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGAGAGTGTCGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ5_PTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5964TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGTACAGTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0144_V004_J005_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCAGAGTGTCGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ5_PTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5965TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGGATCATCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0145_V005_J005_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGAGAGTGTCCCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5966 ORF_IGLJ5_PCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTATTGGCGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0146_V006_J005_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGAAGAGTGTCGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ5_PTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 5967TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCAGGCTTGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0147_V007_J005_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTAGAGTGTCGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ5_PCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 5968CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCACACACGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0148_V008_J005_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGAAGAGTGTCATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ5_PCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 5969CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTAGACGGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0149_V009_J005_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTAGAGTGTCGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ5_PCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5970CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCCAGCTCTTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0150_V010_J005_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATAAGAGTGTCATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ5_PCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 5971CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGAGCGATAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0151_V011_J005_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGAAGAGTGTCGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ5_PCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 5972CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGCATCTGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0152_V012_J005_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACAGAGTGTCGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 5973 ORF_IGLJ5_PCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTGCTACACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0153_V013_J005_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCAAGAGTGTCCTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ5_PCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 5974AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCAACTGCCAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0154_V014_J005_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGAGAGTGTCTTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 5975 FP_IGLJ5_PGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTTGGACTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0155_V015_J005_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACAGAGTGTCTTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ5_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5976AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGTAGACACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0156_V016_J005_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACAGAGTGTCTTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ5_PACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 5977AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCCACTGTACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0157_V017_J005_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCAGAGTGTCTTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ5_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 5978AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGATGATCCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0158_V018_J005_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATAAGAGTGTCTTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ5_PGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 5979AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCCGCCAATAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0159_V019_J005_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTAGAGTGTCTTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ5_PACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 5980AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTCAAGCCTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0160_V020_J005_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTAGAGTGTCCCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 5981 FP_IGLJ5_PCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCACGTGTGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0161_V021_J005_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTAAGAGTGTCTTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ5_PGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 5982AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTCCGTCTAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0162_V022_J005_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGAGAGTGTCCTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ5_PTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 5983CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCAAGAGCTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0163_V023_J005_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCAGAGTGTCTGCTGACTCAGCCC SEQ ID NO: 03_IGLJ5_PCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 5984TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTATCGCTCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0164_V024_J005_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCAGAGTGTCCTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ5_PGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 5985AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTCAGATGCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0165_V025_J005_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCAAGAGTGTCCTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ5_PGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 5986AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGTGTAGCAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0166_V026_J005_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTAGAGTGTCTGTGCTGACTCAGC SEQ ID NO: 37_IGLJ5_PCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 5987CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTGGCAGTTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0167_V027_J005_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAAAGAGTGTCTGTGCTGACTCAGC SEQ ID NO: 39_IGLJ5_PCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 5988CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCCAGTCCAAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0168_V028_J005_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGAGAGTGTCTGTGCTGACTCAGC SEQ ID NO: 45_IGLJ5_PCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 5989CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTACGTACGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0169_V029_J005_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGAAGAGTGTCTGACTCAGCCATCT SEQ ID NO: 52_IGLJ5_PTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 5990GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCAGTACCGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0170_V030_J005_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGAGAGTGTCAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ5_PAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5991AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCATCCATGGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0171_V031_J005_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTAGAGTGTCAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 5992 FP_IGLJ5_PAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCGTAGCAGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0172_V032_J005_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTAGAGTGTCGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ5_PACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 5993TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCATCTTCGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0173_V033_J005_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTAGAGTGTCTGACTCAGCCACCT SEQ ID NO: 49_IGLJ5_PTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 5994GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCTCCACAGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0174_V034_J005_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCAGAGTGTCTGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 5995 FP_IGLJ5_PCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCATGACACCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0175_V035_J005_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGAAGAGTGTCCGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 5996 ORF_IGLJ5_PCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGAAGAGTGTCGTCGACTGTTTGGTGAGGGGACGGAGCTGACCGTCCTAGATGAGTCTTTTCCCCCTCCTTCCCTGGTCTCCCCAAGGTACTGGGAAATTTTCTGCTGCTTTTGTTCCTTCACGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGL_0176_V001_J006_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACGTTCCGAAGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ6_FTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 5997TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTAGGAGACGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0177_V002_J006_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACGTTCCGAACCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ6_FGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 5998CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGTGTCTACGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0178_V003_J006_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGGTTCCGAAGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ6_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 5999TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGTACAGTGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0179_V004_J006_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCGTTCCGAAGGTCCTGGGCCCAG SEQ ID NO: 47_IGLJ6_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 6000TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGGATCATCGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0180_V005_J006_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGGTTCCGAACCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 6001 ORF_IGLJ6_FCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTATTGGCGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0181_V006_J006_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGAGTTCCGAAGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ6_FTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 6002TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTAGGCTTGAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0182_V007_J006_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTGTTCCGAAGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ6_FCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 6003CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTACACACGTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0183_V008_J006_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGAGTTCCGAAATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ6_FCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 6004CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTAGACGGAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0184_V009_J006_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTGTTCCGAAGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ6_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 6005CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTCAGCTCTTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0185_V010_J006_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATAGTTCCGAAATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ6_FCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 6006CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGAGCGATAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0186_V011_J006_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGAGTTCCGAAGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ6_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 6007CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGCATCTGAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0187_V012_J006_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACGTTCCGAAGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 6008 ORF_IGLJ6_FCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTGCTACACGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0188_V013_J006_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCAGTTCCGAACTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ6_FCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 6009AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTAACTGCCAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0189_V014_J006_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGGTTCCGAATTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 6010 FP_IGLJ6_FGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTTGGACTGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0190_V015_J006_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACGTTCCGAATTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ6_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 6011AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGTAGACACGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0191_V016_J006_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACGTTCCGAATTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ6_FACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 6012AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTCACTGTACGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0192_V017_J006_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCGTTCCGAATTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ6_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 6013AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGATGATCCGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0193_V018_J006_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATAGTTCCGAATTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ6_FGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 6014AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTCGCCAATAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0194_V019_J006_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTGTTCCGAATTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ6_FACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 6015AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTCAAGCCTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0195_V020_J006_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTGTTCCGAACCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 6016 FP_IGLJ6_FCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTACGTGTGTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0196_V021_J006_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTAGTTCCGAATTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ6_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 6017AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTCCGTCTAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0197_V022_J006_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGGTTCCGAACTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ6_FTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 6018CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTAAGAGCTGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0198_V023_J006_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCGTTCCGAATGCTGACTCAGCCC SEQ ID NO: 03_IGLJ6_FCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 6019TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTATCGCTCGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0199_V024_J006_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCGTTCCGAACTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ6_FGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 6020AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTCAGATGCGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0200_V025_J006_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCAGTTCCGAACTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ6_FGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 6021AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGTGTAGCAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0201_V026_J006_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTGTTCCGAATGTGCTGACTCAGC SEQ ID NO: 37_IGLJ6_FCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 6022CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTGGCAGTTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0202_V027_J006_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAAGTTCCGAATGTGCTGACTCAGC SEQ ID NO: 39_IGLJ6_FCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 6023CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTCAGTCCAAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0203_V028_J006_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGGTTCCGAATGTGCTGACTCAGC SEQ ID NO: 45_IGLJ6_FCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 6024CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTACGTACGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0204_V029_J006_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGAGTTCCGAATGACTCAGCCATCT SEQ ID NO: 52_IGLJ6_FTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 6025GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTAGTACCGAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0205_V030_J006_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGGTTCCGAAAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ6_FAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 6026AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTATCCATGGGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0206_V031_J006_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTGTTCCGAAAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 6027 FP_IGLJ6_FAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTGTAGCAGTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0207_V032_J006_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTGTTCCGAAGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ6_FACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 6028TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTATCTTCGTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0208_V033_J006_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTGTTCCGAATGACTCAGCCACCT SEQ ID NO: 49_IGLJ6_FTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 6029GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTTCCACAGTGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0209_V034_J006_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCGTTCCGAATGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 6030 FP_IGLJ6_FCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTATGACACCGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0210_V035_J006_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGAGTTCCGAACGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 6031 ORF_IGLJ6_FCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGAGTTCCGAAGTCGACTGTTCGGCAGTGGCACCAAGGTGACCGTCCTCGGTGAGTCCCCTTTTCTATTCTTTTGGGTCTAGGGTGAGATCTGGGGAGACTTTTCTGTCCTTTCTGTCTTCACGAGTTCCGAACTGATGGCGCGAGG GAGGChsIGL_0211_V001_J007_IGLV01-GCCTTGCCAGCCCGCTCAGTAGGAGACCGTTACTTGGTCCTGGGCCCAG SEQ ID NO: 36_IGLJ7_FTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGG 6032TCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTAAACTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGATAGGAGACCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTAGGAGACCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0212_V002_J007_IGLV01-GCCTTGCCAGCCCGCTCAGGTGTCTACCGTTACTTCCTGGGCCCAGTCT SEQ ID NO: 40_IGLJ7_FGTCGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 6033CCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGAGTGTCTACCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGTGTCTACCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0213_V003_J007_IGLV01-GCCTTGCCAGCCCGCTCAGGTACAGTGCGTTACTTGGTCCTGGGCCCAG SEQ ID NO: 44_IGLJ7_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 6034TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGTACAGTGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGTACAGTGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0214_V004_J007_IGLV01-GCCTTGCCAGCCCGCTCAGGGATCATCCGTTACTTGGTCCTGGGCCCAG SEQ ID NO: 47_TGLJ7_FTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG 6035TCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGATGAGGATCATCCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGGATCATCCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0215_V005_J007_IGLV01-GCCTTGCCAGCCCGCTCAGTATTGGCGCGTTACTTCCTGGGCCCAGTCT SEQ ID NO: 50-GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCA 6036 ORF_IGLJ7_FCCATCTCCTGCACTGGGAGCAGCTCCAACATTGGGGCGGGTTATGTTGTACATTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCAATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGACTCCAGTCTGAGGATGAGGCTGATTATTACTGCAAAGCATGGGATAACAGCCTGATGATATTGGCGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTATTGGCGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0216_V006_J007_IGLV01-GCCTTGCCAGCCCGCTCAGAGGCTTGACGTTACTTGGTCCTGGGCCCAG SEQ ID NO: 51_IGLJ7_FTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGG 6037TCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGATGAAGGCTTGACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGAGGCTTGACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0217_V007_J007_IGLV02-GCCTTGCCAGCCCGCTCAGACACACGTCGTTACTTGTCCTGGGCCCAGT SEQ ID NO: 08_IGLJ7_FCTGCCCTGACTCAGCCTCCCTCCGCGTCCAGGTCTCCTGGACAGTCAGT 6038CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAATGAACACACGTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGACACACGTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0218_V008_J007_IGLV02-GCCTTGCCAGCCCGCTCAGTAGACGGACGTTACTTATCCTGGGCTCAGT SEQ ID NO: 11_IGLJ7_FCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGT 6039CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCTATGATAGACGGACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTAGACGGACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0219_V009_J007_IGLV02-GCCTTGCCAGCCCGCTCAGCAGCTCTTCGTTACTTGTCCTGGGCCCAGT SEQ ID NO: 14_IGLJ7_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 6040CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGACAGCTCTTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGCAGCTCTTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0220_V010_J007_IGLV02-GCCTTGCCAGCCCGCTCAGGAGCGATACGTTACTTATCCTGGGCTCAGT SEQ ID NO: 18_IGLJ7_FCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGT 6041CACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGGTCCAAGTCTGGCAACACGGCCTCCCTGACCACCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGTGAGAGCGATACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGAGCGATACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0221_V011_J007_IGLV02-GCCTTGCCAGCCCGCTCAGGCATCTGACGTTACTTGTCCTGGGCCCAGT SEQ ID NO: 23_IGLJ7_FCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGAT 6042CACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGTGAGCATCTGACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGCATCTGACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0222_V012_J007_IGLV02-GCCTTGCCAGCCCGCTCAGTGCTACACCGTTACTTGTCCTGGGCCCAGT SEQ ID NO: 33-CTGCCCTGACTCAGCCTCCTTTTGTGTCCGGGGCTCCTGGACAGTCGGT 6043 ORF_IGLJ7_FCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGGGATTATGATCATGTCTTCTGGTACCAAAAGCGTCTCAGCACTACCTCCAGACTCCTGATTTACAATGTCAATACTCGGCCTTCAGGGATCTCTGACCTCTTCTCAGGCTCCAAGTCTGGCAACATGGCTTCCCTGACCATCTCTGGGCTCAAGTCCGAGGTTGAGGCTAATTATCACTGCAGCTTATATTCAAGTAGTTATGATGCTACACCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTGCTACACCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0223_V013_J007_IGLV03-GCCTTGCCAGCCCGCTCAGAACTGCCACGTTACTTCTCTCCTGTAGGAT SEQ ID NO: 01_IGLJ7_FCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCC 6044AGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGTGAAACTGCCACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGAACTGCCACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0224_V014_J007_IGLV03-GCCTTGCCAGCCCGCTCAGTTGGACTGCGTTACTTTTTTCTTGCAGGTT SEQ ID NO: 09-CTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCT 6045 FP_IGLJ7_FGGGACAGGCGGCCAGGATTACCTGTGGGGGAAACAACCTTGGATATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAACAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGTGATTGGACTGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTTGGACTGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0225_V015_J007_IGLV03-GCCTTGCCAGCCCGCTCAGGTAGACACCGTTACTTTTGCAGTCTCTGAG SEQ ID NO: 10_IGLJ7_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 6046AAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTACTCAACAGACAGCAGTGGTATGAGTAGACACCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGTAGACACCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0226_V016_J007_IGLV03-GCCTTGCCAGCCCGCTCAGCACTGTACCGTTACTTTTGCAGGCTCTGCG SEQ ID NO: 12_IGLJ7_FACCTCCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCAC 6047AGATGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTAAAGCTGTGCACTGGTACCAGCAAAAGCCAGGCCAGGACCCTGTGCTGGTCATCTATAGCGATAGCAACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACCCAGGGAACACCGCCACCCTAACCATCAGCAGGATCGAGGCTGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGACACTGTACCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGCACTGTACCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0227_V017_J007_IGLV03-GCCTTGCCAGCCCGCTCAGGATGATCCCGTTACTTTTGCAGGCTCTGAG SEQ ID NO: 16_IGLJ7_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCTAGGAC 6048AGATGGCCAGGATCACCTGCTCTGGAGAAGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGTTCCCTGTGCTGGTGATATATAAAGACAGCGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAATAGTCACATTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCTATCAGCAGACAGCAGTGGTATGAGATGATCCCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGATGATCCCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0228_V018_J007_IGLV03-GCCTTGCCAGCCCGCTCAGCGCCAATACGTTACTTTTGCAGGTTCTGTG SEQ ID NO: 19_IGLJ7_FGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGAC 6049AGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTATGACGCCAATACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGCGCCAATACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0229_V019_J007_IGLV03-GCCTTGCCAGCCCGCTCAGTCAAGCCTCGTTACTTTTGCAGGCTCTGTG SEQ ID NO: 21_IGLJ7_FACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAA 6050AGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGACAGTAGTAGTGTGATCAAGCCTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTCAAGCCTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0230_V020_J007_IGLV03-GCCTTGCCAGCCCGCTCAGACGTGTGTCGTTACTTCCTCTCTTGCAGGC SEQ ID NO: 22-TCTGTTGCCTCCTATGAGCTGACACAGCTACCCTCGGTGTCAGTGTCCC 6051 FP_IGLJ7_FCAGGACAGAAAGCCAGGATCACCTGCTCTGGAGATGTACTGGGGAAAAATTATGCTGACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGAGTTGGTGATATACGAAGATAGTGAGCGGTACCCTGGAATCCCTGAACGATTCTCTGGGTCCACCTCAGGGAACACGACCACCCTGACCATCAGCAGGGTCCTGACCGAAGACGAGGCTGACTATTACTGTTTGTCTGGGAATGAGGTGAACGTGTGTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGACGTGTGTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0231_V021_J007_IGLV03-GCCTTGCCAGCCCGCTCAGTCCGTCTACGTTACTTTTGCAGGCTCTGAG SEQ ID NO: 25_IGLJ7_FGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGAC 6052AGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGATGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTATGATCCGTCTACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTCCGTCTACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0232_V022_J007_IGLV03-GCCTTGCCAGCCCGCTCAGAAGAGCTGCGTTACTTCTTTTCTTGCAGTC SEQ ID NO: 27_IGLJ7_FTCTGTGGCCTCCTATGAGCTGACACAGCCATCCTCAGTGTCAGTGTCTC 6053CGGGACAGACAGCCAGGATCACCTGCTCAGGAGATGTACTGGCAAAAAAATATGCTCGGTGGTTCCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTGATTTATAAAGACAGTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAGCTCAGGGACCACAGTCACCTTGACCATCAGCGGGGCCCAGGTTGAGGATGAGGCTGACTATTACTGTTACTCTGCGGCTGACATGAAAGAGCTGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGAAGAGCTGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0233_V023_J007_IGLV04-GCCTTGCCAGCCCGCTCAGTATCGCTCCGTTACTTTGCTGACTCAGCCC SEQ ID NO: 03_IGLJ7_FCCGTCTGCATCTGCCTTGCTGGGAGCCTCGATCAAGCTCACCTGCACCC 6054TAAGCAGTGAGCACAGCACCTACACCATCGAATGGTATCAACAGAGACCAGGGAGGTCCCCCCAGTATATAATGAAGGTTAAGAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCCGATCGCTTCATGGGCTCCAGTTCTGGGGCTGACCGCTACCTCACCTTCTCCAACCTCCAGTCTGACGATGAGGCTGAGTATCACTGTGGAGAGAGCCACACGATTGATGGCCAAGTCGTGATATCGCTCCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTATCGCTCCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0234_V024_J007_IGLV04-GCCTTGCCAGCCCGCTCAGTCAGATGCCGTTACTTCTCTCTCCCAGCCT SEQ ID NO: 60_IGLJ7_FGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCA 6055AGCTCACCTGCACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGTATGATCAGATGCCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTCAGATGCCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0235_V025_J007_IGLV04-GCCTTGCCAGCCCGCTCAGGTGTAGCACGTTACTTCTCTCTCCCAGCTT SEQ ID NO: 69_IGLJ7_FGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCA 6056AGCTCACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGGCCCTCGGTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGTGAGTGTAGCACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGTGTAGCACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0236_V026_J007_IGLV05-GCCTTGCCAGCCCGCTCAGTGGCAGTTCGTTACTTTGTGCTGACTCAGC SEQ ID NO: 37_IGLJ7_FCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCAC 6057CTTGCCCAGTGACATCAATGTTGGTAGCTACAACATATACTGGTACCAGCAGAAGCCAGGGAGCCCTCCCAGGTATCTCCTGTACTACTACTCAGACTCAGATAAGGGCCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAATACAGGGATTTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAAGCAATGATGGCAGTTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTGGCAGTTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0237_V027_J007_IGLV05-GCCTTGCCAGCCCGCTCAGCAGTCCAACGTTACTTTGTGCTGACTCAGC SEQ ID NO: 39_IGLJ7_FCAACCTCCCTCTCAGCATCTCCTGGAGCATCAGCCAGATTCACCTGCAC 6058CTTGCGCAGCGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAATCCAGGGAGTCTTCCCCGGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAACCAATGCAGGCCTTTTACTCATCTCTGGGCTCCAGTCTGAAGATGAGGCTGACTATTACTGTGCCATTTGGTACAGCAGTGACAGTCCAACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGCAGTCCAACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0238_V028_J007_IGLV05-GCCTTGCCAGCCCGCTCAGTACGTACGCGTTACTTTGTGCTGACTCAGC SEQ ID NO: 45_IGLJ7_FCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCAC 6059CTTGCGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCACAGCAGTGATACGTACGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTACGTACGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0239_V029_J007_IGLV05-GCCTTGCCAGCCCGCTCAGAGTACCGACGTTACTTTGACTCAGCCATCT SEQ ID NO: 52_IGLJ7_FTCCCATTCTGCATCTTCTGGAGCATCAGTCAGACTCACCTGCATGCTGA 6060GCAGTGGCTTCAGTGTTGGGGACTTCTGGATAAGGTGGTACCAACAAAAGCCAGGGAACCCTCCCCGGTATCTCCTGTACTACCACTCAGACTCCAATAAGGGCCAAGGCTCTGGAGTTCCCAGCCGCTTCTCTGGATCCAACGATGCATCAGCCAATGCAGGGATTCTGCGTATCTCTGGGCTCCAGCCTGAGGATGAGGCTGACTATTACTGTGGTACATGGCACAGCAACTCTATGAAGTACCGACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGAGTACCGACGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0240_V030_J007_IGLV07-GCCTTGCCAGCCCGCTCAGATCCATGGCGTTACTTAGGGTCCAATTCTC SEQ ID NO: 43_IGLJ7_FAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 6061AGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGGTTACTATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGAATCCATGGCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGATCCATGGCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0241_V031_J007_IGLV07-GCCTTGCCAGCCCGCTCAGGTAGCAGTCGTTACTTAGGGTCCAATTCCC SEQ ID NO: 46-AGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGAC 6062 FP_IGLJ7_FAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACAAGCAACAAACACTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTTGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGAGTAGCAGTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGGTAGCAGTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0242_V032_J007_IGLV08-GCCTTGCCAGCCCGCTCAGATCTTCGTCGTTACTTGAGTGGATTCTCAG SEQ ID NO: 61_IGLJ7_FACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAG 6063TCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATTGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGTGAATCTTCGTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGATCTTCGTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0243_V033_J007_IGLV09-GCCTTGCCAGCCCGCTCAGTCCACAGTCGTTACTTTGACTCAGCCACCT SEQ ID NO: 49_IGLJ7_FTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGA 6064GCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAAGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGTGATCCACAGTCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGTCCACAGTCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0244_V034_J007_IGLV10-GCCTTGCCAGCCCGCTCAGATGACACCCGTTACTTTGTCAGTGGTCCAG SEQ ID NO: 54-GCAGGGCTGACTCAGCCACCCTCGGTCTCCAAGGGCTTGAGACAGACCG 6065 FP_IGLJ7_FCCACACTCACCTGCACTGGGAACAGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCCTGAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTATCTGCATCCAGGTCAGGAAACACAGCCTCCCTGACCATTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTACTGCTCAGCATGGGACAGCAGCCTCATGAATGACACCCGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGATGACACCCGTTACTTCTGATGGCGCGAGG GAGGChsIGL_0245_V035_J007_IGLV11-GCCTTGCCAGCCCGCTCAGCTTCACGACGTTACTTCGTGCTGACTCAGC SEQ ID NO: 55-CGCCCTCTCTGTCTGCATCCCCGGGAGCAACAGCCAGACTCCCCTGCAC 6066 ORF_IGLJ7_FCCTGAGCAGTGACCTCAGTGTTGGTGGTAAAAACATGTTCTGGTACCAGCAGAAGCCAGGGAGCTCTCCCAGGTTATTCCTGTATCACTACTCAGACTCAGACAAGCAGCTGGGACCTGGGGTCCCCAGTCGAGTCTCTGGCTCCAAGGAGACCTCAAGTAACACAGCGTTTTTGCTCATCTCTGGGCTCCAGCCTGAGGACGAGGCCGATTATTACTGCCAGGTGTACGAAAGTAGTGACTTCACGACGTTACTTGTCGACTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTAAGTCTCCCCGCTTCTCTCCTCTTTGAGATCCCAAGTTAAACACGGGGAGTTTTTCCCTTTCCTGCTTCACGACGTTACTTCTGATGGCGCGAGG GAGGC Bias ControlSequences for hs-IgK Name Sequence SEQ ID NO hsIGK_0001_V001_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGTTCCGAAGACACTCTCCAATCTCAGGTGC SEQ ID NO: 05-CAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCT 6067 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGAGTTCCGAAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGTTCCGAAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0002_V002_J001_IGKV1-GCCTTGCCAGCCCGCTCAGCGTTACTTGACACTCTCCAATCTCAGGTGC SEQ ID NO: 06-CAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6068 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTGACGTTACTTGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGACGTTACTTGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0003_V003_J001_IGKV1-GCCTTGCCAGCCCGCTCAGTAGGAGACGACACTCTCCAATCTCAGGTGC SEQ ID NO: 08-CAGATGTGCCATCCGGATGACCCAGTCTCCATCCTCATTCTCTGCATCT 6069 F_IGKJ1ACAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCTGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGATAGGAGACGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATAGGAGACGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0004_V004_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGTGTCTACGACACTCTCCAATCTCAGGTGC SEQ ID NO: 09-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCT 6070 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTGAGTGTCTACGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGTGTCTACGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0005_V005_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGTACAGTGGACACTCTCCAATCTCAGGTTC SEQ ID NO: 12-CAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATCT 6071 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTGAGTACAGTGGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGTACAGTGGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0006_V006_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGGATCATCGACACTCTCCAATCTCAGGTGC SEQ ID NO: 13-CAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6072 FP_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGATATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAATTGAGGATCATCGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGGATCATCGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0007_V007_J001_IGKV1-GCCTTGCCAGCCCGCTCAGTATTGGCGGACACTCTCCAATCTCAGGTGC SEQ ID NO: 16-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCT 6073 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGATATTGGCGGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATATTGGCGGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0008_V008_J001_IGKV1-GCCTTGCCAGCCCGCTCAGAGGCTTGAGACACTCTCCAATCTCAGGTGC SEQ ID NO: 17-CAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6074 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTGAAGGCTTGAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAAGGCTTGAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0009_V009_J001_IGKV1-GCCTTGCCAGCCCGCTCAGACACACGTGACACTCTCTAATATCAGATAC SEQ ID NO: 27-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6075 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTTGAACACACGTGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAACACACGTGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0010_V010_J001_IGKV1-GCCTTGCCAGCCCGCTCAGTAGACGGAGACACTCTCTAATCGCAGGTGC SEQ ID NO: 33-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6076 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTGATAGACGGAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATAGACGGAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0011_V011_J001_IGKV1-GCCTTGCCAGCCCGCTCAGCAGCTCTTGACACTCTCCAATCTCAGGTGC SEQ ID NO: 37-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6077 O_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGTGAGTCAGGGCATTAGCAGTTATTTAAATTGGTATCGGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATAGTGCATCCAATTTGCAATCTGGAGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACGGTCAACGGACTTACAATTGACAGCTCTTGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGACAGCTCTTGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0012_V012_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGAGCGATAGACACTCTCCAATCTCAGGTGC SEQ ID NO: 39-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6078 FP_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTGAGAGCGATAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGAGCGATAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0013_V013_J001_IGKV1-GCCTTGCCAGCCCGCTCAGGCATCTGAGACACTCTCCAATCTCAGGTAC SEQ ID NO: NL1-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6079 F_IGKJ1GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGAGCATCTGAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGCATCTGAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0014_V014_J001_IGKV2-GCCTTGCCAGCCCGCTCAGTGCTACACGACACTCTAGTGGGGATATTGT SEQ ID NO: 24-GATGACCCAGACTCCACTCTCCTCACCTGTCACCCTTGGACAGCCGGCC 6080 F_IGKJ1TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCTACACAATGATGCTACACGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATGCTACACGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0015_V015_J001_IGKV2-GCCTTGCCAGCCCGCTCAGAACTGCCAGACACTCTAGTGGGGATATTGT SEQ ID NO: 28-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCC 6081 F_IGKJ1TCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAATGAAACTGCCAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAAACTGCCAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0016_V016_J001_IGKV2-GCCTTGCCAGCCCGCTCAGTTGGACTGGACACTCTAGTGCGGATATTGT SEQ ID NO: 29-GATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCC 6082 FP_IGKJ1TCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGAATGCAAGGTATACACTGATTGGACTGGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATTGGACTGGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0017_V017_J001_IGKV2-GCCTTGCCAGCCCGCTCAGGTAGACACGACACTCTAGTGGGGATGTTGT SEQ ID NO: 30-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCC 6083 F_IGKJ1TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGAGTAGACACGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGTAGACACGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0018_V018_J001_IGKV2-GCCTTGCCAGCCCGCTCAGCACTGTACGACACTCTGAGGATATTGTGAT SEQ ID NO: 40-GACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCC 6084 F_IGKJ1ATCTCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTGACACTGTACGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGACACTGTACGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0019_V019_J001_IGKV3-GCCTTGCCAGCCCGCTCAGGATGATCCGACACTCTATCTCAGATACCAC SEQ ID NO: 07-CGGAGAAATTGTAATGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA 6085 F_IGKJ1GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTATCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGGATTATAACTGAGATGATCCGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAGATGATCCGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0020_V020_J001_IGKV3-GCCTTGCCAGCCCGCTCAGCGCCAATAGACACTCTCCAATTTCAGATAC SEQ ID NO: 11-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6086 F_IGKJ1CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGACGCCAATAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGACGCCAATAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0021_V021_J001_IGKV3-GCCTTGCCAGCCCGCTCAGTCAAGCCTGACACTCTCCAATTTCAGATAC SEQ ID NO: 15-CACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT 6087 F_IGKJ1CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGATCAAGCCTGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATCAAGCCTGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0022_V022_J001_IGKV3-GCCTTGCCAGCCCGCTCAGACGTGTGTGACACTCTATCTCAGATACCAC SEQ ID NO: 20-CGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA 6088 F_IGKJ1GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTGAACGTGTGTGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAACGTGTGTGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0023_V023_J001_IGKV3-GCCTTGCCAGCCCGCTCAGTCCGTCTAGACACTCTCCAATTTCAGATAC SEQ ID NO: NL4-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6089 FNG_IGKJ1CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGCCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGATCCGTCTAGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATCCGTCTAGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0024_V024_J001_IGKV4-GCCTTGCCAGCCCGCTCAGAAGAGCTGGACACTCTGGGGACATCGTGAT SEQ ID NO: 01-GACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACC 6090 F_IGKJ1ATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTGAAAGAGCTGGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGAAAGAGCTGGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0025_V025_J001_IGKV5-GCCTTGCCAGCCCGCTCAGTATCGCTCGACACTCTCCATAATCAGATAC SEQ ID NO: 02-CAGGGCAGAAACGACACTCACGCAGTCTCCAGCATTCATGTCAGCGACT 6091 F_IGKJ1CCAGGAGACAAAGTCAACATCTCCTGCAAAGCCAGCCAAGACATTGATGATGATATGAACTGGTACCAACAGAAACCAGGAGAAGCTGCTATTTTCATTATTCAAGAAGCTACTACTCTCGTTCCTGGAATCCCACCTCGATTCAGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACATAGAATCTGAGGATGCTGCATATTACTTCTGTCTACAACATGATAATTGATATCGCTCGACACTCTGTCGACCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGTGAGTAGAATTTAAACTTTGCTTCCTCAGTTGTCTGTGTCTTCTGTTCCCTGTGTCTATGAAGTGATATCGCTCGACACTCTCTGATGGCGCGAGG GAGGChsIGK_0026_V001_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGTTCCGAATTCGGAACCCAATCTCAGGTGC SEQ ID NO: 05-CAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCT 6092 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGAGTTCCGAATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGTTCCGAATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0027_V002_J002_IGKV1-GCCTTGCCAGCCCGCTCAGCGTTACTTTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 06-CAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6093 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTGACGTTACTTTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGCGTTACTTTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0028_V003_J002_IGKV1-GCCTTGCCAGCCCGCTCAGTAGGAGACTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 08-CAGATGTGCCATCCGGATGACCCAGTCTCCATCCTCATTCTCTGCATCT 6094 F_IGKJ2ACAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCTGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGATAGGAGACTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTAGGAGACTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0029_V004_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGTGTCTACTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 09-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCT 6095 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTGAGTGTCTACTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGTGTCTACTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0030_V005_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGTACAGTGTTCGGAACCCAATCTCAGGTTC SEQ ID NO: 12-CAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATCT 6096 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTGAGTACAGTGTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGTACAGTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0031_V006_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGGATCATCTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 13-CAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6097 FP_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGATATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAATTGAGGATCATCTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGGATCATCTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0032_V007_J002_IGKV1-GCCTTGCCAGCCCGCTCAGTATTGGCGTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 16-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCT 6098 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGATATTGGCGTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTATTGGCGTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0033_V008_J002_IGKV1-GCCTTGCCAGCCCGCTCAGAGGCTTGATTCGGAACCCAATCTCAGGTGC SEQ ID NO: 17-CAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6099 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTGAAGGCTTGATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGAGGCTTGATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0034_V009_J002_IGKV1-GCCTTGCCAGCCCGCTCAGACACACGTTTCGGAACCTAATATCAGATAC SEQ ID NO: 27-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6100 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTTGAACACACGTTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGACACACGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0035_V010_J002_IGKV1-GCCTTGCCAGCCCGCTCAGTAGACGGATTCGGAACCTAATCGCAGGTGC SEQ ID NO: 33-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6101 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTGATAGACGGATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTAGACGGATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0036_V011_J002_IGKV1-GCCTTGCCAGCCCGCTCAGCAGCTCTTTTCGGAACCCAATCTCAGGTGC SEQ ID NO: 37-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6102 O_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGTGAGTCAGGGCATTAGCAGTTATTTAAATTGGTATCGGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATAGTGCATCCAATTTGCAATCTGGAGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACGGTCAACGGACTTACAATTGACAGCTCTTTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGCAGCTCTTTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0037_V012_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGAGCGATATTCGGAACCCAATCTCAGGTGC SEQ ID NO: 39-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6103 FP_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTGAGAGCGATATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGAGCGATATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0038_V013_J002_IGKV1-GCCTTGCCAGCCCGCTCAGGCATCTGATTCGGAACCCAATCTCAGGTAC SEQ ID NO: NL1-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6104 F_IGKJ2GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGAGCATCTGATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGCATCTGATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0039_V014_J002_IGKV2-GCCTTGCCAGCCCGCTCAGTGCTACACTTCGGAACAGTGGGGATATTGT SEQ ID NO: 24-GATGACCCAGACTCCACTCTCCTCACCTGTCACCCTTGGACAGCCGGCC 6105 F_IGKJ2TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCTACACAATGATGCTACACTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTGCTACACTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0040_V015_J002_IGKV2-GCCTTGCCAGCCCGCTCAGAACTGCCATTCGGAACAGTGGGGATATTGT SEQ ID NO: 28-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCC 6106 F_IGKJ2TCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAATGAAACTGCCATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGAACTGCCATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0041_V016_J002_IGKV2-GCCTTGCCAGCCCGCTCAGTTGGACTGTTCGGAACAGTGCGGATATTGT SEQ ID NO: 29-GATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCC 6107 FP_IGKJ2TCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGAATGCAAGGTATACACTGATTGGACTGTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTTGGACTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0042_V017_J002_IGKV2-GCCTTGCCAGCCCGCTCAGGTAGACACTTCGGAACAGTGGGGATGTTGT SEQ ID NO: 30-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCC 6108 F_IGKJ2TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGAGTAGACACTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGTAGACACTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0043_V018_J002_IGKV2-GCCTTGCCAGCCCGCTCAGCACTGTACTTCGGAACGAGGATATTGTGAT SEQ ID NO: 40-GACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCC 6109 F_IGKJ2ATCTCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTGACACTGTACTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGCACTGTACTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0044_V019_J002_IGKV3-GCCTTGCCAGCCCGCTCAGGATGATCCTTCGGAACATCTCAGATACCAC SEQ ID NO: 07-CGGAGAAATTGTAATGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA 6110 F_IGKJ2GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTATCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGGATTATAACTGAGATGATCCTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGGATGATCCTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0045_V020_J002_IGKV3-GCCTTGCCAGCCCGCTCAGCGCCAATATTCGGAACCCAATTTCAGATAC SEQ ID NO: 11-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6111 F_IGKJ2CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGACGCCAATATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGCGCCAATATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0046_V021_J002_IGKV3-GCCTTGCCAGCCCGCTCAGTCAAGCCTTTCGGAACCCAATTTCAGATAC SEQ ID NO: 15-CACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT 6112 F_IGKJ2CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGATCAAGCCTTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTCAAGCCTTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0047_V022_J002_IGKV3-GCCTTGCCAGCCCGCTCAGACGTGTGTTTCGGAACATCTCAGATACCAC SEQ ID NO: 20-CGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA 6113 F_IGKJ2GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTGAACGTGTGTTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGACGTGTGTTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0048_V023_J002_IGKV3-GCCTTGCCAGCCCGCTCAGTCCGTCTATTCGGAACCCAATTTCAGATAC SEQ ID NO: NL4-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6114 FNG_IGKJ2CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGCCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGATCCGTCTATTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTCCGTCTATTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0049_V024_J002_IGKV4-GCCTTGCCAGCCCGCTCAGAAGAGCTGTTCGGAACGGGGACATCGTGAT SEQ ID NO: 01-GACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACC 6115 F_IGKJ2ATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTGAAAGAGCTGTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGAAGAGCTGTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0050_V025_J002_IGKV5-GCCTTGCCAGCCCGCTCAGTATCGCTCTTCGGAACCCATAATCAGATAC SEQ ID NO: 02-CAGGGCAGAAACGACACTCACGCAGTCTCCAGCATTCATGTCAGCGACT 6116 F_IGKJ2CCAGGAGACAAAGTCAACATCTCCTGCAAAGCCAGCCAAGACATTGATGATGATATGAACTGGTACCAACAGAAACCAGGAGAAGCTGCTATTTTCATTATTCAAGAAGCTACTACTCTCGTTCCTGGAATCCCACCTCGATTCAGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACATAGAATCTGAGGATGCTGCATATTACTTCTGTCTACAACATGATAATTGATATCGCTCTTCGGAACGTCGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGTAAGTACTTTTTTCCACTGATTCTTCACTGTTGCTAATTAGTTTACTTTGTGTTCCTTTGTGTGGTATCGCTCTTCGGAACCTGATGGCGCGAGG GAGGChsIGK_0051_V001_J003_IGKV1-GCCTTGCCAGCCCGCTCAGGTTCCGAAAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 05-CAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCT 6117 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGAGTTCCGAAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGTTCCGAAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0052_V002_J003_IGKV1-GCCTTGCCAGCCCGCTCAGCGTTACTTAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 06-CAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6118 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTGACGTTACTTAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGCGTTACTTAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0053_V003_J003_IGKV1-GCCTTGCCAGCCCGCTCAGTAGGAGACAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 08-CAGATGTGCCATCCGGATGACCCAGTCTCCATCCTCATTCTCTGCATCT 6119 F_IGKJ3ACAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCTGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGATAGGAGACAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTAGGAGACAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0054_V004_J003_IGKV1-GCCTTGCCAGCCCGCTCAGGTGTCTACAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 09-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCT 6120 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTGAGTGTCTACAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGTGTCTACAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0055_V005_J003_IGKV1-GCCTTGCCAGCCCGCTCAGGTACAGTGAAGTAACGCCAATCTCAGGTTC SEQ ID NO: 12-CAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATCT 6121 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTGAGTACAGTGAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGTACAGTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0056_V006_J003_IGKV1-GCCTTGCCAGCCCGCTCAGGGATCATCAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 13-CAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6122 FP_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGATATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAATTGAGGATCATCAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGGATCATCAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0057_V007_J003_IGKV1-GCCTTGCCAGCCCGCTCAGTATTGGCGAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 16-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCT 6123 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGATATTGGCGAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTATTGGCGAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0058_V008_J003_IGKV1-GCCTTGCCAGCCCGCTCAGAGGCTTGAAAGTAACGCCAATCTCAGGTGC SEQ ID NO: 17-CAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6124 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTGAAGGCTTGAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGAGGCTTGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0059_V009_J003_IGKV1-GCCTTGCCAGCCCGCTCAGACACACGTAAGTAACGCTAATATCAGATAC SEQ ID NO: 27-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6125 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTTGAACACACGTAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGACACACGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0060_V010_J003_IGKV1-GCCTTGCCAGCCCGCTCAGTAGACGGAAAGTAACGCTAATCGCAGGTGC SEQ ID NO: 33-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6126 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTGATAGACGGAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTAGACGGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0061_V011_J003_IGKV1-37-GCCTTGCCAGCCCGCTCAGCAGCTCTTAAGTAACGCCAATCTCAGGTGC SEQ ID NO: O_IGKJ3CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6127GTAGGAGACAGAGTCACCATCACTTGCCGGGTGAGTCAGGGCATTAGCAGTTATTTAAATTGGTATCGGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATAGTGCATCCAATTTGCAATCTGGAGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACGGTCAACGGACTTACAATTGACAGCTCTTAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGCAGCTCTTAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0062_V012_J003_IGKV1-39-GCCTTGCCAGCCCGCTCAGGAGCGATAAAGTAACGCCAATCTCAGGTGC SEQ ID NO: FP_IGKJ3CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6128GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTGAGAGCGATAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGAGCGATAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0063_V013_J003_IGKV1-GCCTTGCCAGCCCGCTCAGGCATCTGAAAGTAACGCCAATCTCAGGTAC SEQ ID NO: NL1-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6129 F_IGKJ3GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGAGCATCTGAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGCATCTGAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0064_V014_J003_IGKV2-24-GCCTTGCCAGCCCGCTCAGTGCTACACAAGTAACGAGTGGGGATATTGT SEQ ID NO: F_IGKJ3GATGACCCAGACTCCACTCTCCTCACCTGTCACCCTTGGACAGCCGGCC 6130TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCTACACAATGATGCTACACAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTGCTACACAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0065_V015_J003_IGKV2-28-GCCTTGCCAGCCCGCTCAGAACTGCCAAAGTAACGAGTGGGGATATTGT SEQ ID NO: F_IGKJ3GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCC 6131TCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAATGAAACTGCCAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGAACTGCCAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0066_V016_J003_IGKV2-29-GCCTTGCCAGCCCGCTCAGTTGGACTGAAGTAACGAGTGCGGATATTGT SEQ ID NO: FP_IGKJ3GATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCC 6132TCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGAATGCAAGGTATACACTGATTGGACTGAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTTGGACTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0067_V017_J003_IGKV2-30-GCCTTGCCAGCCCGCTCAGGTAGACACAAGTAACGAGTGGGGATGTTGT SEQ ID NO: F_IGKJ3GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCC 6133TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGAGTAGACACAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGTAGACACAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0068_V018_J003_IGKV2-40-GCCTTGCCAGCCCGCTCAGCACTGTACAAGTAACGGAGGATATTGTGAT SEQ ID NO: F_IGKJ3GACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCC 6134ATCTCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTGACACTGTACAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGCACTGTACAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0069_V019_J003_IGKV3-07-GCCTTGCCAGCCCGCTCAGGATGATCCAAGTAACGATCTCAGATACCAC SEQ ID NO: F_IGKJ3CGGAGAAATTGTAATGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA 6135GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTATCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGGATTATAACTGAGATGATCCAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGGATGATCCAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0070_V020_J003_IGKV3-11-GCCTTGCCAGCCCGCTCAGCGCCAATAAAGTAACGCCAATTTCAGATAC SEQ ID NO: F_IGKJ3CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6136CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGACGCCAATAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGCGCCAATAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0071_V021_J003_IGKV3-15-GCCTTGCCAGCCCGCTCAGTCAAGCCTAAGTAACGCCAATTTCAGATAC SEQ ID NO: F_IGKJ3CACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT 6137CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGATCAAGCCTAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTCAAGCCTAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0072_V022_J003_IGKV3-20-GCCTTGCCAGCCCGCTCAGACGTGTGTAAGTAACGATCTCAGATACCAC SEQ ID NO: F_IGKJ3CGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA 6138GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTGAACGTGTGTAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGACGTGTGTAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0073_V023_J003_IGKV3-GCCTTGCCAGCCCGCTCAGTCCGTCTAAAGTAACGCCAATTTCAGATAC SEQ ID NO: NL4-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6139 FNG_IGKJ3CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGCCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGATCCGTCTAAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTCCGTCTAAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0074_V024_J003_IGKV4-01-GCCTTGCCAGCCCGCTCAGAAGAGCTGAAGTAACGGGGGACATCGTGAT SEQ ID NO: F_IGKJ3GACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACC 6140ATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTGAAAGAGCTGAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGAAGAGCTGAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0075_V025_J003_IGKV5-02-GCCTTGCCAGCCCGCTCAGTATCGCTCAAGTAACGCCATAATCAGATAC SEQ ID NO: F_IGKJ3CAGGGCAGAAACGACACTCACGCAGTCTCCAGCATTCATGTCAGCGACT 6141CCAGGAGACAAAGTCAACATCTCCTGCAAAGCCAGCCAAGACATTGATGATGATATGAACTGGTACCAACAGAAACCAGGAGAAGCTGCTATTTTCATTATTCAAGAAGCTACTACTCTCGTTCCTGGAATCCCACCTCGATTCAGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACATAGAATCTGAGGATGCTGCATATTACTTCTGTCTACAACATGATAATTGATATCGCTCAAGTAACGGTCGACCTTTCGGCCCTGGGACCAAAGTGGATATCAAACGTAAGTACATCTGTCTCAATTATTCGTGAGATTTTAGTGCCATTGTATCATTTGTGCAAGTTTTGTGTATCGCTCAAGTAACGCTGATGGCGCGAGG GAGGChsIGK_0076_V001_J004_IGKV1-05-GCCTTGCCAGCCCGCTCAGGTTCCGAAGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCT 6142GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGAGTTCCGAAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGTTCCGAAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0077_V002_J004_IGKV1-06-GCCTTGCCAGCCCGCTCAGCGTTACTTGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6143GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTGACGTTACTTGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATCGTTACTTGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0078_V003_J004_IGKV1-08-GCCTTGCCAGCCCGCTCAGTAGGAGACGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAGATGTGCCATCCGGATGACCCAGTCTCCATCCTCATTCTCTGCATCT 6144ACAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCTGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGATAGGAGACGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTAGGAGACGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0079_V004_J004_IGKV1-09-GCCTTGCCAGCCCGCTCAGGTGTCTACGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCT 6145GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTGAGTGTCTACGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGTGTCTACGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0080_V005_J004_IGKV1-12-GCCTTGCCAGCCCGCTCAGGTACAGTGGTCTCCTACCAATCTCAGGTTC SEQ ID NO: F_IGKJ4CAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATCT 6146GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTGAGTACAGTGGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGTACAGTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0081_V006_J004_IGKV1-13-GCCTTGCCAGCCCGCTCAGGGATCATCGTCTCCTACCAATCTCAGGTGC SEQ ID NO: FP_IGKJ4CAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6147GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGATATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAATTGAGGATCATCGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGGATCATCGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0082_V007_J004_IGKV1-16-GCCTTGCCAGCCCGCTCAGTATTGGCGGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCT 6148GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGATATTGGCGGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTATTGGCGGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0083_V008_J004_IGKV1-17-GCCTTGCCAGCCCGCTCAGAGGCTTGAGTCTCCTACCAATCTCAGGTGC SEQ ID NO: F_IGKJ4CAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6149GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTGAAGGCTTGAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATAGGCTTGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0084_V009_J004_IGKV1-27-GCCTTGCCAGCCCGCTCAGACACACGTGTCTCCTACTAATATCAGATAC SEQ ID NO: F_IGKJ4CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6150GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTTGAACACACGTGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATACACACGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0085_V010_J004_IGKV1-33-GCCTTGCCAGCCCGCTCAGTAGACGGAGTCTCCTACTAATCGCAGGTGC SEQ ID NO: F_IGKJ4CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6151GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTGATAGACGGAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTAGACGGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0086_V011_J004_IGKV1-37-GCCTTGCCAGCCCGCTCAGCAGCTCTTGTCTCCTACCAATCTCAGGTGC SEQ ID NO: O_IGKJ4CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6152GTAGGAGACAGAGTCACCATCACTTGCCGGGTGAGTCAGGGCATTAGCAGTTATTTAAATTGGTATCGGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATAGTGCATCCAATTTGCAATCTGGAGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACGGTCAACGGACTTACAATTGACAGCTCTTGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATCAGCTCTTGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0087_V012_J004_IGKV1-39-GCCTTGCCAGCCCGCTCAGGAGCGATAGTCTCCTACCAATCTCAGGTGC SEQ ID NO: FP_IGKJ4CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6153GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTGAGAGCGATAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGAGCGATAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0088_V013_J004_IGKV1-GCCTTGCCAGCCCGCTCAGGCATCTGAGTCTCCTACCAATCTCAGGTAC SEQ ID NO: NL1-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6154 F_IGKJ4GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGAGCATCTGAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGCATCTGAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0089_V014_J004_IGKV2-GCCTTGCCAGCCCGCTCAGTGCTACACGTCTCCTAAGTGGGGATATTGT SEQ ID NO: 24-GATGACCCAGACTCCACTCTCCTCACCTGTCACCCTTGGACAGCCGGCC 6155 F_IGKJ4TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCTACACAATGATGCTACACGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTGCTACACGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0090_V015_J004_IGKV2-GCCTTGCCAGCCCGCTCAGAACTGCCAGTCTCCTAAGTGGGGATATTGT SEQ ID NO: 28-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCC 6156 F_IGKJ4TCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAATGAAACTGCCAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATAACTGCCAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0091_V016_J004_IGKV2-GCCTTGCCAGCCCGCTCAGTTGGACTGGTCTCCTAAGTGCGGATATTGT SEQ ID NO: 29-GATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCC 6157 FP_IGKJ4TCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGAATGCAAGGTATACACTGATTGGACTGGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTTGGACTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0092_V017_J004_IGKV2-GCCTTGCCAGCCCGCTCAGGTAGACACGTCTCCTAAGTGGGGATGTTGT SEQ ID NO: 30-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCC 6158 F_IGKJ4TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGAGTAGACACGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGTAGACACGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0093_V018_J004_IGKV2-GCCTTGCCAGCCCGCTCAGCACTGTACGTCTCCTAGAGGATATTGTGAT SEQ ID NO: 40-GACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCC 6159 F_IGKJ4ATCTCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTGACACTGTACGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATCACTGTACGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0094_V019_J004_IGKV3-GCCTTGCCAGCCCGCTCAGGATGATCCGTCTCCTAATCTCAGATACCAC SEQ ID NO: 07-CGGAGAAATTGTAATGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA 6160 F_IGKJ4GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTATCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGGATTATAACTGAGATGATCCGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATGATGATCCGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0095_V020_J004_IGKV3-GCCTTGCCAGCCCGCTCAGCGCCAATAGTCTCCTACCAATTTCAGATAC SEQ ID NO: 11-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6161 F_IGKJ4CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGACGCCAATAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATCGCCAATAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0096_V021_J004_IGKV3-GCCTTGCCAGCCCGCTCAGTCAAGCCTGTCTCCTACCAATTTCAGATAC SEQ ID NO: 15-CACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT 6162 F_IGKJ4CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGATCAAGCCTGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTCAAGCCTGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0097_V022_J004_IGKV3-GCCTTGCCAGCCCGCTCAGACGTGTGTGTCTCCTAATCTCAGATACCAC SEQ ID NO: 20-CGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA 6163 F_IGKJ4GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTGAACGTGTGTGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATACGTGTGTGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0098_V023_J004_IGKV3-GCCTTGCCAGCCCGCTCAGTCCGTCTAGTCTCCTACCAATTTCAGATAC SEQ ID NO: NL4-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6164 FNG_IGKJ4CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGCCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGATCCGTCTAGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTCCGTCTAGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0099_V024_J004_IGKV4-GCCTTGCCAGCCCGCTCAGAAGAGCTGGTCTCCTAGGGGACATCGTGAT SEQ ID NO: 01-GACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACC 6165 F_IGKJ4ATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTGAAAGAGCTGGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATAAGAGCTGGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0100_V025_J004_IGKV5-GCCTTGCCAGCCCGCTCAGTATCGCTCGTCTCCTACCATAATCAGATAC SEQ ID NO: 02-CAGGGCAGAAACGACACTCACGCAGTCTCCAGCATTCATGTCAGCGACT 6166 F_IGKJ4CCAGGAGACAAAGTCAACATCTCCTGCAAAGCCAGCCAAGACATTGATGATGATATGAACTGGTACCAACAGAAACCAGGAGAAGCTGCTATTTTCATTATTCAAGAAGCTACTACTCTCGTTCCTGGAATCCCACCTCGATTCAGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACATAGAATCTGAGGATGCTGCATATTACTTCTGTCTACAACATGATAATTGATATCGCTCGTCTCCTAGTCGACCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTAAGTGCACTTTCCTAATGCTTTTTCTTATAAGGTTTTAAATTTGGAGCGTTTTTGTGTTTGAGATTATCGCTCGTCTCCTACTGATGGCGCGAGG GAGGChsIGK_0101_V001_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGTTCCGAAAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 05-CAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCT 6167 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGAGTTCCGAAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGTTCCGAAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0102_V002_J005_IGKV1-GCCTTGCCAGCCCGCTCAGCGTTACTTAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 06-CAGATGTGCCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6168 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTGACGTTACTTAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTCGTTACTTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0103_V003_J005_IGKV1-GCCTTGCCAGCCCGCTCAGTAGGAGACAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 08-CAGATGTGCCATCCGGATGACCCAGTCTCCATCCTCATTCTCTGCATCT 6169 F_IGKJ5ACAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCTGCCTGCAGTCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGATAGGAGACAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTAGGAGACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0104_V004_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGTGTCTACAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 09-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCT 6170 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTGAGTGTCTACAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGTGTCTACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0105_V005_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGTACAGTGAGAGTGTCCCAATCTCAGGTTC SEQ ID NO: 12-CAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATCT 6171 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTGAGTACAGTGAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGTACAGTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0106_V006_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGGATCATCAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 13-CAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6172 FP_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGCCTGATATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAATTGAGGATCATCAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGGATCATCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0107_V007_J005_IGKV1-GCCTTGCCAGCCCGCTCAGTATTGGCGAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 16-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCACTGTCTGCATCT 6173 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTGATATTGGCGAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTATTGGCGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0108_V008_J005_IGKV1-GCCTTGCCAGCCCGCTCAGAGGCTTGAAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 17-CAGGTGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6174 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAGCATAATAGTTGAAGGCTTGAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTAGGCTTGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0109_V009_J005_IGKV1-GCCTTGCCAGCCCGCTCAGACACACGTAGAGTGTCCTAATATCAGATAC SEQ ID NO: 27-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6175 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACAGTTGAACACACGTAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTACACACGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0110_V010_J005_IGKV1-GCCTTGCCAGCCCGCTCAGTAGACGGAAGAGTGTCCTAATCGCAGGTGC SEQ ID NO: 33-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6176 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATTGATAGACGGAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTAGACGGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0111_V011_J005_IGKV1-GCCTTGCCAGCCCGCTCAGCAGCTCTTAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 37-CAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6177 O_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGTGAGTCAGGGCATTAGCAGTTATTTAAATTGGTATCGGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATAGTGCATCCAATTTGCAATCTGGAGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACGGTCAACGGACTTACAATTGACAGCTCTTAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTCAGCTCTTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0112_V012_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGAGCGATAAGAGTGTCCCAATCTCAGGTGC SEQ ID NO: 39-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6178 FP_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTGAGAGCGATAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGAGCGATAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0113_V013_J005_IGKV1-GCCTTGCCAGCCCGCTCAGGCATCTGAAGAGTGTCCCAATCTCAGGTAC SEQ ID NO: NL1-CAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 6179 F_IGKJ5GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCTGGGACGGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTATTATAGTTGAGCATCTGAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGCATCTGAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0114_V014_J005_IGKV2-GCCTTGCCAGCCCGCTCAGTGCTACACAGAGTGTCAGTGGGGATATTGT SEQ ID NO: 24-GATGACCCAGACTCCACTCTCCTCACCTGTCACCCTTGGACAGCCGGCC 6180 F_IGKJ5TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCTACACAATGATGCTACACAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTGCTACACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0115_V015_J005_IGKV2-GCCTTGCCAGCCCGCTCAGAACTGCCAAGAGTGTCAGTGGGGATATTGT SEQ ID NO: 28-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCC 6181 F_IGKJ5TCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAATGAAACTGCCAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTAACTGCCAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0116_V016_J005_IGKV2-GCCTTGCCAGCCCGCTCAGTTGGACTGAGAGTGTCAGTGCGGATATTGT SEQ ID NO: 29-GATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCC 6182 FP_IGKJ5TCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAAAATCAGCCGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGAATGCAAGGTATACACTGATTGGACTGAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTTGGACTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0117_V017_J005_IGKV2-GCCTTGCCAGCCCGCTCAGGTAGACACAGAGTGTCAGTGGGGATGTTGT SEQ ID NO: 30-GATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCC 6183 F_IGKJ5TCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGAGTAGACACAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGTAGACACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0118_V018_J005_IGKV2-GCCTTGCCAGCCCGCTCAGCACTGTACAGAGTGTCGAGGATATTGTGAT SEQ ID NO: 40-GACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCC 6184 F_IGKJ5ATCTCCTGCAGGTCTAGTCAGAGCCTCTTGGATAGTGATGATGGAAACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTGACACTGTACAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTCACTGTACAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0119_V019_J005_IGKV3-GCCTTGCCAGCCCGCTCAGGATGATCCAGAGTGTCATCTCAGATACCAC SEQ ID NO: 07-CGGAGAAATTGTAATGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA 6185 F_IGKJ5GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTATCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGGATTATAACTGAGATGATCCAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTGATGATCCAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0120_V020_J005_IGKV3-GCCTTGCCAGCCCGCTCAGCGCCAATAAGAGTGTCCCAATTTCAGATAC SEQ ID NO: 11-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6186 F_IGKJ5CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGACGCCAATAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTCGCCAATAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0121_V021_J005_IGKV3-GCCTTGCCAGCCCGCTCAGTCAAGCCTAGAGTGTCCCAATTTCAGATAC SEQ ID NO: 15-CACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCT 6187 F_IGKJ5CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGATCAAGCCTAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTCAAGCCTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0122_V022_J005_IGKV3-GCCTTGCCAGCCCGCTCAGACGTGTGTAGAGTGTCATCTCAGATACCAC SEQ ID NO: 20-CGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA 6188 F_IGKJ5GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTGAACGTGTGTAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTACGTGTGTAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0123_V023_J005_IGKV3-GCCTTGCCAGCCCGCTCAGTCCGTCTAAGAGTGTCCCAATTTCAGATAC SEQ ID NO: NL4-CACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT 6189 FNG_IGKJ5CCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGGGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGCCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGATCCGTCTAAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTCCGTCTAAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0124_V024_J005_IGKV4-GCCTTGCCAGCCCGCTCAGAAGAGCTGAGAGTGTCGGGGACATCGTGAT SEQ ID NO: 01-GACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACC 6190 F_IGKJ5ATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTGAAAGAGCTGAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTAAGAGCTGAGAGTGTCCTGATGGCGCGAGG GAGGChsIGK_0125_V025_J005_IGKV5-GCCTTGCCAGCCCGCTCAGTATCGCTCAGAGTGTCCCATAATCAGATAC SEQ ID NO: 02-CAGGGCAGAAACGACACTCACGCAGTCTCCAGCATTCATGTCAGCGACT 6191 F_IGKJ5CCAGGAGACAAAGTCAACATCTCCTGCAAAGCCAGCCAAGACATTGATGATGATATGAACTGGTACCAACAGAAACCAGGAGAAGCTGCTATTTTCATTATTCAAGAAGCTACTACTCTCGTTCCTGGAATCCCACCTCGATTCAGTGGCAGCGGGTATGGAACAGATTTTACCCTCACAATTAATAACATAGAATCTGAGGATGCTGCATATTACTTCTGTCTACAACATGATAATTGATATCGCTCAGAGTGTCGTCGACCCTTCGGCCAAGGGACACGACTGGAGATTAAACGTAAGTAATTTTTCACTATTGTCTTCTGAAATTTGGGTCTGATGGCCAGTATTGACTTTTAGAGGCTTATCGCTCAGAGTGTCCTGATGGCGCGAGG GAGGC Gene specificprimer Primer With Universal Target sequence Sequence SEQ ID NO PrimerSequences for hs-TCR13-P10 TCRBV01 GAATGCCCTGACAGCTCTCGCGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: TTATA AATGCCCTGACAGCTCTCGCTTAT 6192A TCRBV02 CTCAGAGAAGTCTGAAATATT GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO:CGATGATCAATTCTCAGTTG TCAGAGAAGTCTGAAATATTCGAT 6193 GATCAATTCTCAGTTGTCRBV03-1 CCAAATCGMTTCTCACCTAAA GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO:TCTCCAGACAAAG CAAATCGMTTCTCACCTAAATCTC 6194 CAGACAAAG TCRBV03-2CACCTGACTCTCCAGACAAAG GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CTCATACCTGACTCTCCAGACAAAGCTCA 6195 T TCRBV04- CCTGAATGCCCCAACAGCTCTGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: 1/2/3 C CTGAATGCCCCAACAGCTCTC 6196TCRBV05-1 GATTCTCAGGGCGCCAGTTCT GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: CTAATTCTCAGGGCGCCAGTTCTCTA 6197 TCRBV05-2 CCTAATTGATTCTCAGCTCACGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CACGTCCATA CTAATTGATTCTCAGCTCACCACG6198 TCCATA TCRBV05-3 TCAGGGCGCCAGTTCCATG GGGCTGGCAAGCCACGTTTGGTGT SEQID NO: CAGGGCGCCAGTTCCATG 6199 TCRBV05-4 TCCTAGATTCTCAGGTCTCCAGGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO: GTTCCCTA CCTAGATTCTCAGGTCTCCAGTTC6200 CCTA TCRBV05-5 GAGGAAACTTCCCTGATCGAT GGGCTGGCAAGCCACGTTTGGTGG SEQID NO: TCTCAGC AGGAAACTTCCCTGATCGATTCTC 6201 AGC TCRBV05-6CAACTTCCCTGATCGATTCTC GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: AGGTCAAACTTCCCTGATCGATTCTCAGGT 6202 CA TCRBV05-7 AGGAAACTTCCCTGATCAATTGGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: CTCAGGTCA GGAAACTTCCCTGATCAATTCTCA6203 GGTCA TCRBV05-8 GGAAACTTCCCTCCTAGATTT GGGCTGGCAAGCCACGTTTGGTGG SEQID NO: TCAGGTCG GAAACTTCCCTCCTAGATTTTCAG 6204 GTCG TCRBV06-1CCCCAATGGCTACAATGTCTC GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CAGATTCCCAATGGCTACAATGTCTCCAGA 6205 TT TCRBV06- GGAGAAGTCCCCAATGGCTACGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: 2/3 AA GAGAGGTCCCTGATGGCTACAA 6206TCRBV06-4 TCCCTGATGGTTATAGTGTCT GGGCTGGCAAGCCACGTTTGGTGT CCAGAGCCCCTGATGGTTATAGTGTCTCCAG SEQ ID NO: AGC 6207 TCRBV06-5GGAGAAGTCCCCAATGGCTAC GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: AATGTCGAGAAGTCCCCAATGGCTACAATG 6208 TC TCRBV06-6 AAAGGAGAAGTCCCGAATGGCGGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: TACAA AAGGAGAAGTCCCGAATGGCTACA 6209A TCRBV06-7 GTTCCCAATGGCTACAATGTC GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO:TCCAGATC TTCCCAATGGCTACAATGTCTCCA 6210 GATC TCRBV06-8GAAGTCCCCAATGGCTACAAT GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: GTCTCTAGATTAAGTCCCCAATGGCTACAATGTCT 6211 CTAGATT TCRBV06-9 GAGAAGTCCCCGATGGCTACAGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: ATGTA AGAAGTCCCCGATGGCTACAATGT 6212A TCRBV07-1 GTGATCGGTTCTCTGCACAGA GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO:GGT TGATCGGTTCTCTGCACAGAGGT 6213 TCRBV07-2 CGCTTCTCTGCAGAGAGGACTGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: GG GCTTCTCTGCAGAGAGGACTGG 6214TCRBV07-3 GGTTCTTTGCAGTCAGGCCTG GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: AGTTCTTTGCAGTCAGGCCTGA 6215 TCRBV07-4 CAGTGGTCGGTTCTCTGCAGAGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: G AGTGGTCGGTTCTCTGCAGAG 6216TCRBV07-5 GCTCAGTGATCAATTCTCCAC GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO:AGAGAGGT CTCAGTGATCAATTCTCCACAGAG 6217 AGGT TCRBV07-TTCTCTGCAGAGAGGCCTGAG GGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO: 6/7 GTCTCTGCAGAGAGGCCTGAGG 6218 TCRBV07-8 CCCAGTGATCGCTTCTTTGCAGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: GAAA CCAGTGATCGCTTCTTTGCAGAAA 6219TCRBV07-9 CTGCAGAGAGGCCTAAGGGAT GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CTTGCAGAGAGGCCTAAGGGATCT 6220 TCRBV08-1 GAAGGGTACAATGTCTCTGGAGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: AACAAACTCAAGAAGGGTACAATGTCTCTGGAAACA 6221 AACTCAAG TCRBV08-2 GGGGTACTGTGTTTCTTGAAAGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: CAAGCTTGAG GGGTACTGTGTTTCTTGAAACAAG6222 CTTGAG TCRBV09 CAGTTCCCTGACTTGCACTCT GGGCTGGCAAGCCACGTTTGGTGC SEQID NO: GAACTAAAC AGTTCCCTGACTTGCACTCTGAAC 6223 TAAAC TCRBV10-1ACTAACAAAGGAGAAGTCTCA GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: GATGGCTACAGCTAACAAAGGAGAAGTCTCAGATG 6224 GCTACAG TCRBV10-2 AGATAAAGGAGAAGTCCCCGAGGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: TGGCTA GATAAAGGAGAAGTCCCCGATGGC 6225TA TCRBV10-3 GATACTGACAAAGGAGAAGTC GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO:TCAGATGGCTATAG ATACTGACAAAGGAGAAGTCTCAG 6226 ATGGCTATAG TCRBV11-CTAAGGATCGATTTTCTGCAG GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: 1/2/3 AGAGGCTCTAAGGATCGATTTTCTGCAGAGAG 6227 GCTC TCRBV12-1 TTGATTCTCAGCACAGATGCCGGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO: TGATGT TGATTCTCAGCACAGATGCCTGAT 6228GT TCRBV12-2 ATTCTCAGCTGAGAGGCCTGA GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO:TGG TTCTCAGCTGAGAGGCCTGATGG 6229 TCRBV12- GGATCGATTCTCAGCTAAGATGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: 3/4 GCCTAATGCGATCGATTCTCAGCTAAGATGCCT 6230 AATGC TCRBV12-5 CTCAGCAGAGATGCCTGATGCGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: AACTTTA TCAGCAGAGATGCCTGATGCAACT6231 TTA TCRBV13 CTGATCGATTCTCAGCTCAAC GGGCTGGCAAGCCACGTTTGGTGC SEQ IDNO: AGTTCAGT TGATCGATTCTCAGCTCAACAGTT 6232 CAGT TCRBV14TAGCTGAAAGGACTGGAGGGA GGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO: CGTATAGCTGAAAGGACTGGAGGGACGTA 6233 T TCRBV15 CCAGGAGGCCGAACACTTCTTGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: TCT CAGGAGGCCGAACACTTCTTTCT 6234TCRBV16 GCTAAGTGCCTCCCAAATTCA GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: CCCTCTAAGTGCCTCCCAAATTCACCCT 6235 TCRBV17 CACAGCTGAAAGACCTAACGGGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: AACGT ACAGCTGAAAGACCTAACGGAACG 6236T TCRBV18 CTGCTGAATTTCCCAAAGAGG GGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: GCCTGCTGAATTTCCCAAAGAGGGCC 6237 TCRBV19 AGGGTACAGCGTCTCTCGGGGGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: GGGTACAGCGTCTCTCGGG 6238 TCRBV20GCCTGACCTTGTCCACTCTGA GGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: CACCTGACCTTGTCCACTCTGACA 6239 TCRBV21 ATGAGCGATTTTTAGCCCAATGGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: GCTCCA TGAGCGATTTTTAGCCCAATGCTC 6240CA TCRBV22 TGAAGGCTACGTGTCTGCCAA GGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO: GAGGAAGGCTACGTGTCTGCCAAGAG 6241 TCRBV23 CTCATCTCAATGCCCCAAGAAGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CGC TCATCTCAATGCCCCAAGAACGC 6242TCRBV24 AGATCTCTGATGGATACAGTG GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO:TCTCTCGACA GATCTCTGATGGATACAGTGTCTC 6243 TCGACA TCRBV25AGATCTTTCCTCTGAGTCAAC GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: AGTCTCCAGAATAGATCTTTCCTCTGAGTCAACAGTC 6244 TCCAGAATA TCRBV26 CACTGAAAAAGGAGATATCTCGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: TGAGGGGTATCATGACTGAAAAAGGAGATATCTCTGAG 6245 GGGTATCATG TCRBV27 GTTCCTGAAGGGTACAAAGTCGGGCTGGCAAGCCACGTTTGGTGG SEQ ID NO: TCTCGAAAAG TTCCTGAAGGGTACAAAGTCTCTC6246 GAAAAG TCRBV28 CTGAGGGGTACAGTGTCTCTA GGGCTGGCAAGCCACGTTTGGTGC SEQID NO: GAGAGA TGAGGGGTACAGTGTCTCTAGAGA 6247 GA TCRBV29AGCCGCCCAAACCTAACATTC GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: TCAAGCCGCCCAAACCTAACATTCTCAA 6248 TCRBV30 CCCAGGACCGGCAGTTCAGGGCTGGCAAGCCACGTTTGGTGC SEQ ID NO: CCAGGACCGGCAGTTCA 6249 TCRBVATTGATTAGAGACATATCCCTA GGGCTGGCAAGCCACGTTTGGTGT SEQ ID NO:TTGAAAATATTTCCTGGCA TGATTAGAGACATATCCCTATTGA 6250 AAATATTTCCTGGCA TCRBVBAGATGCCCTGAGTCAGCATAG GGGCTGGCAAGCCACGTTTGGTGA SEQ ID NO: TCATTCTAACGATGCCCTGAGTCAGCATAGTCAT 6251 TCTAAC TCRBJ1-1 GTCTTACCTACAACTGTGAGTCCGGGAGCTGCATGTGTCAGAGGG SEQ ID NO: CTGGTGCC TCTTACCTACAACTGTGAGTCTGG6252 TGCC TCRBJ1-2 CCTTACCTACAACGGTTAACC CCGGGAGCTGCATGTGTCAGAGGC SEQ IDNO: TGGTCCC CTTACCTACAACGGTTAACCTGGT 6253 CCC TCRBJ1-3CTTACTCACCTACAACAGTGA CCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: GCCAACTTCCTTACTCACCTACAACAGTGAGCCA 6254 ACTTCC TCRBJ1-4 ATACCCAAGACAGAGAGCTGGCCGGGAGCTGCATGTGTCAGAGGA SEQ ID NO: GTTCC TACCCAAGACAGAGAGCTGGGTTC 6255C TCRBJ1-5 AACTTACCTAGGATGGAGAGT CCGGGAGCTGCATGTGTCAGAGGA SEQ ID NO:CGAGTCCC ACTTACCTAGGATGGAGAGTCGAG 6256 TCCC TCRBJ1-6CTGTCACAGTGAGCCTGGTCC CCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: CTGTCACAGTGAGCCTGGTCCC 6257 TCRBJ2-1 CACGGTGAGCCGTGTCCCCCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: ACGGTGAGCCGTGTCCC 6258 TCRBJ2-2CCAGTACGGTCAGCCTAGAGC CCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: CCAGTACGGTCAGCCTAGAGCC 6259 TCRBJ2-3 CACTGTCAGCCGGGTGCCCCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: ACTGTCAGCCGGGTGCC 6260 TCRBJ2-4CACTGAGAGCCGGGTCCC CCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: ACTGAGAGCCGGGTCCC6261 TCRBJ2-5 ACCAGGAGCCGCGTGCC CCGGGAGCTGCATGTGTCAGAGGA SEQ ID NO:CCAGGAGCCGCGTGCC 6262 TCRBJ2-6 CACGGTCAGCCTGCTGCCCCGGGAGCTGCATGTGTCAGAGGC SEQ ID NO: ACTGTCAGCCGGGTGCC 6263 TCRBJ2-7GACCGTGAGCCTGGTGCC CCGGGAGCTGCATGTGTCAGAGGG SEQ ID NO: ACCGTGAGCCTGGTGCC6264 Primer Sequences for hs-IGH-D IGHD1- CGCTAGCTGGGGCTCACAGTGGGGCTGGCAAGCCACGTTTGGTGCG SEQ ID NO: 14_ver10 CTCACTAGCTGGGGCTCACAGTGCTCA 6265 IGHD2- CACTGGGCTCAGAGTCCTCTCGGGCTGGCAAGCCACGTTTGGTGCA SEQ ID NO: 02_ver10 CCACACCTGGGCTCAGAGTCCTCTCCCACAC 6266 IGHD2- CCTATACAGCACTGGGCTCAGGGGCTGGCAAGCCACGTTTGGTGCC SEQ ID NO: 15_ver10 AGTCCTCTCTGAGACTATACAGCACTGGGCTCAGAGTCCT 6267 CTCTGAGAC IGHD3- CCTAAGCCAGGGGCAGACCCGGGGCTGGCAAGCCACGTTTGGTGCC SEQ ID NO: 03_ver10 AGT TAAGCCAGGGGCAGACCCGAGT6268 IGHD3- ACAGTGTCACAGAGTCCATCA GGGCTGGCAAGCCACGTTTGGTGAC SEQ ID NO:10_ver10 AAAACCCATGCCTGG AGTGTCACAGAGTCCATCAAAAACC 6269 CATGCCTGG IGHD3-CACTATCCACATAAGCGAGGG GGGCTGGCAAGCCACGTTTGGTGCA SEQ ID NO: 16_ver10ACAGACCCGAGT CTATCCACATAAGCGAGGGACAGAC 6270 CCGAGT IGHD4-TGCCCTCGATGGCAGGCGGA GGGCTGGCAAGCCACGTTTGGTGTG SEQ ID NO: 04_ver10CCCTCGATGGCAGGCGGA 6271 IGHD4- CCTCTTCCAGGACAGTCCTCAGGGCTGGCAAGCCACGTTTGGTGCC SEQ ID NO: 11_ver10 GTGGCATCACAGTCTTCCAGGACAGTCCTCAGTGGCA 6272 TCACAG IGHD4- CAGACCCACCTGCCCTCAATGGGGCTGGCAAGCCACGTTTGGTGCA SEQ ID NO: 23_ver10 GCAGGACCCACCTGCCCTCAATGGCAG 6273 IGHD5- TCTCCAGGGAGACACTGTGCAGGGCTGGCAAGCCACGTTTGGTGTC SEQ ID NO: 12_ver10 TGTCTGGTACCTAATCCAGGGAGACACTGTGCATGTCTG 6274 GTACCTAA IGHD5- GGGACACAGTGCATGTCTGGTGGGCTGGCAAGCCACGTTTGGTGGG SEQ ID NO: 24_ver10 CCCTGAGACACAGTGCATGTCTGGTCCCTGA 6275 IGHD6- GGACCCCTATTCCAGACACCAGGGCTGGCAAGCCACGTTTGGTGGG SEQ ID NO: 13_ver10 GACAGAGGCACCCCTATTCCAGACACCAGACAGA 6276 GGC IGHD6- CCCCACTCCAGACACCAGACAGGGCTGGCAAGCCACGTTTGGTGCC SEQ ID NO: 19_ver10 GAGGGCCACTCCAGACACCAGACAGAGGG 6277 IGHD7- GGGGTCTCCCACGTGTTTTGGGGGCTGGCAAGCCACGTTTGGTGGG SEQ ID NO: 27_ver10 GGCTAACGGTCTCCCACGTGTTTTGGGGCTAA 6278 C IGHD1- GCTAGCTGGGGCTGCCAGTCCGGGCTGGCAAGCCACGTTTGGTGGC SEQ ID NO: 26_ver10 TCA TAGCTGGGGCTGCCAGTCCTCA6279 Primer Sequences for IGK and IGL IGK_V_01- TCTGCATCTGTAGGAGACAGGGCTGGCAAGCCACGTTTGG SEQ ID 05_F_D10 GAGTCACCATCACTTGTGTCTGCATCTGTAGGAGACA NO: 6280 GAGTCACCATCACTTG IGK_V_01-TCTGCATCTACAGGAGACA GGGCTGGCAAGCCACGTTTGG SEQ ID 08_F_D10GAGTCACCATCACTTG TGTCTGCATCTACAGGAGACA NO: 6281 GAGTCACCATCACTTGIGK_V_01- CTGCATCTGTAAGGAGACA GGGCTGGCAAGCCACGTTTGG SEQ ID 35_P_D10GTGTCACCATCACTTG TGCTGCATCTGTAAGGAGACA NO: 6282 GTGTCACCATCACTTGIGK_V_1D- TCTGCATCTACAGGAGACA GGGCTGGCAAGCCACGTTTGG SEQ ID 08_F_D10GAGTCACCATCAGTTG TGTCTGCATCTACAGGAGACA NO: 6283 GAGTCACCATCAGTTGIGK_V_1D- ACTGCATCTGTAGGAGAGA GGGCTGGCAAGCCACGTTTGG SEQ ID 22_P_D10GAGTCACCATCACTTG TGACTGCATCTGTAGGAGAGA NO: 6284 GAGTCACCATCACTTGIGK_V_1D- GCATCTGTAAGGAGACAGC GGGCTGGCAAGCCACGTTTGG SEQ ID 35_P_D10GTCACCATCACTTG TGGCATCTGTAAGGAGACAGC NO: 6285 GTCACCATCACTTG IGK_V_1D-GTCTGCATCTGTAGGAGAC GGGCTGGCAAGCCACGTTTGG SEQ ID 42_F_D10AGAGTCAGTATCATTTG TGGTCTGCATCTGTAGGAGAC NO: 6286 AGAGTCAGTATCATTTGIGK_V_02- GGAGAGCCGGCCTCCATCT GGGCTGGCAAGCCACGTTTGG SEQ ID 04_P_D10 CCTGTGGGAGAGCCGGCCTCCATCT NO: 6287 CCTG IGK_V_02- CCTGGAGAGCCAGCCTCCAGGGCTGGCAAGCCACGTTTGG SEQ ID 10_P_D10 TCTCCTG TGCCTGGAGAGCCAGCCTCCA NO:6288 TCTCCTG IGK_V_02- CTGGAGAGCCGGCCTCCAT GGGCTGGCAAGCCACGTTTGG SEQ ID18_P_D10 CTCTTG TGCTGGAGAGCCGGCCTCCAT NO: 6289 CTCTTG IGK_V_02-TCTTCCTTGGAGAGCCATC GGGCTGGCAAGCCACGTTTGG SEQ ID 19_P_D10 CTCCATTTCCTGTGTCTTCCTTGGAGAGCCATC NO: 6290 CTCCATTTCCTG IGK_V_02-GGACAGCCGGCCTCCATCT GGGCTGGCAAGCCACGTTTGG SEQ ID 24_F_D10 CCTGTGGGACAGCCGGCCTCCATCT NO: 6291 CCTG IGK_V_02- TGGAGAGCCGGCCTCCATCGGGCTGGCAAGCCACGTTTGG SEQ ID 28_F_D10 TCCTG TGTGGAGAGCCGGCCTCCATC NO:6292 TCCTG IGK_V_02- ATAATATTTGTACATAACT GGGCTGGCAAGCCACGTTTGG SEQ ID38_P_D10 TTGTACTTCATCTCCTG TGATAATATTTGTACATAACT NO: 6293TTGTACTTCATCTCCTG IGK_V_2D- CCCCTGGAAAGCCAGCCTC GGGCTGGCAAGCCACGTTTGGSEQ ID 14_P_D10 TATCTCCTG TGCCCCTGGAAAGCCAGCCTC NO: 6294 TATCTCCTGIGK_V_2D- CTCTTCCTTGGAGAGCCAT GGGCTGGCAAGCCACGTTTGG SEQ ID 19_P_D10CCTCCATTTCCTG TGCTCTTCCTTGGAGAGCCAT NO: 6295 CCTCCATTTCCTG IGK_V_2D-GGACAGCCGGCCTCCATCT GGGCTGGCAAGCCACGTTTGG SEQ ID 24_O_D10 CCTTTGGGACAGCCGGCCTCCATCT NO: 6296 CCTT IGK_V_2D- CCTGGAGAGCAGGCCTCCAGGGCTGGCAAGCCACGTTTGG SEQ ID 26_F_D10 TGTCCTG TGCCTGGAGAGCAGGCCTCCA NO:6297 TGTCCTG IGK_V_03- CCAGGGGAAAGAGCCACCC GGGCTGGCAAGCCACGTTTGG SEQ ID07_F_D10 TCTCCTG TGCCAGGGGAAAGAGCCACCC NO: 6298 TCTCCTG IGK_V_03-TCCAGGGGAAAGAGTCACC GGGCTGGCAAGCCACGTTTGG SEQ ID 07_P_D10 CTCTCCTGTGTCCAGGGGAAAGAGTCACC NO: 6299 CTCTCCTG IGK_V_03- TCTTTGTCTCTGGAGAAAAGGGCTGGCAAGCCACGTTTGG SEQ ID 25_P_D10 AAGCCACCCTGACTTGTGTCTTTGTCTCTGGAGAAAA NO: 6300 AAGCCACCCTGACTTG IGK_V_03-TCTCTAGGGGAAAAAGCCA GGGCTGGCAAGCCACGTTTGG SEQ ID 31_P_D10 CCCTCACCTATGTCTCTAGGGGAAAAAGCCA NO: 6301 CCCTCACCTA IGK_V_03- GGGGAAGGAGCCACCCTCAGGGCTGGCAAGCCACGTTTGG SEQ ID 34_P_D10 CCTG TGGGGGAAGGAGCCACCCTCA NO:6302 CCTG IGK_V_04- GGGCGAGAGGGCCACCATC GGGCTGGCAAGCCACGTTTGG SEQ ID01_F_D10 AACTG TGGGGCGAGAGGGCCACCATC NO: 6303 AACTG IGK_V_05-GCGACTCCAGGAGACAAAG GGGCTGGCAAGCCACGTTTGG SEQ ID 02_F_D10 TCAACATCTCCTGTGGCGACTCCAGGAGACAAAG NO: 6304 TCAACATCTCCTG IGK_V_06-CTGTGACTCCAAAGGAGAA GGGCTGGCAAGCCACGTTTGG SEQ ID 21_O_D10AGTCACCATCACCTG TGCTGTGACTCCAAAGGAGAA NO: 6305 AGTCACCATCACCTG IGK_V_6D-ACTCCAGGGGAGAAAGTCA GGGCTGGCAAGCCACGTTTGG SEQ ID 41_F_D10 CCATCACCTGTGACTCCAGGGGAGAAAGTCA NO: 6306 CCATCACCTG IGK_V_07- CAGGACAGAGGGCCACCATGGGCTGGCAAGCCACGTTTGG SEQ ID 03_P_D10 CACCTG TGCAGGACAGAGGGCCACCAT NO:6307 CACCTG IGL_V_R1- GCAGGACACTCACTCCCCC GGGCTGGCAAGCCACGTTTGG SEQ ID20_P_D10 ACCTG TGGCAGGACACTCACTCCCCC NO: 6308 ACCTG IGL_V_R1-TGGGCCAGAGGGTCACCAT GGGCTGGCAAGCCACGTTTGG SEQ ID 63_P_D10 CTCCTGTGTGGGCCAGAGGGTCACCAT NO: 6309 CTCCTG IGL_V_R1- GGGCAGGTGGGTACCAGCTGGGCTGGCAAGCCACGTTTGG SEQ ID 68_P_D10 CCTG TGGGGCAGGTGGGTACCAGCT NO:6310 CCTG IGL_V_R1- CGTGGGACAGAAGGTCACC GGGCTGGCAAGCCACGTTTGG SEQ ID70_P_D10 CTCTCCTG TGCGTGGGACAGAAGGTCACC NO: 6311 CTCTCCTG IGL_V_R4-TCTCTGGGAGCATCTTCCA GGGCTGGCAAGCCACGTTTGG SEQ ID 59_P_D10 GACTCACCTGTGTCTCTGGGAGCATCTTCCA NO: 6312 GACTCACCTG IGL_V_R4- CACCTCCGGATCAGCCAGAGGGCTGGCAAGCCACGTTTGG SEQ ID 64_P_D10 CTCTCCTG TGCACCTCCGGATCAGCCAGA NO:6313 CTCTCCTG IGL_V_R4- CCGGGAGCATGAGCCAGAC GGGCTGGCAAGCCACGTTTGG SEQ ID65_P_D10 TTACCTG TGCCGGGAGCATGAGCCAGAC NO: 6314 TTACCTG IGL_V_R4-CTCTGCACATCTGAGAAAT GGGCTGGCAAGCCACGTTTGG SEQ ID 66- GCTATAAGACTTCCCTGTGCTCTGCACATCTGAGAAAT NO: 6315 1_P_D10 GCTATAAGACTTCCCTG IGL_V_R5-TGTGGGAGCCTCGGTCAAG GGGCTGGCAAGCCACGTTTGG SEQ ID 58_P_D10 CTTACCTCTGTGTGGGAGCCTCGGTCAAG NO: 6316 CTTACCTC IGL_V_01- CCCAGGCAGAGGGTCACCAGGGCTGGCAAGCCACGTTTGG SEQ ID 36_F_D10 TCTCCTG TGCCCAGGCAGAGGGTCACCA NO:6317 TCTCCTG IGL_V_01- CCAGGGCAGAGGGTCACCA GGGCTGGCAAGCCACGTTTGG SEQ ID40_F_D10 TCTCCTG TGCCAGGGCAGAGGGTCACCA NO: 6318 TCTCCTG IGL_V_01-CCGGGCAGAGGGTCACCAT GGGCTGGCAAGCCACGTTTGG SEQ ID 44_F_D10 CTCTTGTGCCGGGCAGAGGGTCACCAT NO: 6319 CTCTTG IGL_V_01- CCCCAGGACAGAAGGTCACGGGCTGGCAAGCCACGTTTGG SEQ ID 51_F_D10 CATCTCCTG TGCCCCAGGACAGAAGGTCACNO: 6320 CATCTCCTG IGL_V_01- CCACAAGGCAGAGGCTCAC GGGCTGGCAAGCCACGTTTGGSEQ ID 62_P_D10 TGTCTCCTG TGCCACAAGGCAGAGGCTCAC NO: 6321 TGTCTCCTGIGL_V_02- GTCTCCTGGACAGTCAGTC GGGCTGGCAAGCCACGTTTGG SEQ ID 08_F_D10ACCATCTCCTG TGGTCTCCTGGACAGTCAGTC NO: 6322 ACCATCTCCTG IGL_V_02-GTCTCCTGGACAGTCGATC GGGCTGGCAAGCCACGTTTGG SEQ ID 14_F_D10 ACCATCTCCTGTGGTCTCCTGGACAGTCGATC NO: 6323 ACCATCTCCTG IGL_V_02- TCCTGGACAGTCGGTCACCGGGCTGGCAAGCCACGTTTGG SEQ ID 33_O_D10 ATCTCCTG TGTCCTGGACAGTCGGTCACC NO:6324 ATCTCCTG IGL_V_02- CTGGGACTTGGGGTAAACA GGGCTGGCAAGCCACGTTTGG SEQ ID34_P_D10 GTCACCATCTTCTG TGCTGGGACTTGGGGTAAACA NO: 6325 GTCACCATCTTCTGIGL_V_03- CCAGGACAGACAGCCAGCA GGGCTGGCAAGCCACGTTTGG SEQ ID 01_F_D10TCACCTG TGCCAGGACAGACAGCCAGCA NO: 6326 TCACCTG IGL_V_03-CTTTGGGACGTACGGCCAG GGGCTGGCAAGCCACGTTTGG SEQ ID 02_P_D10 GATCATCTGTGCTTTGGGACAGATGGCCAG NO: 6327 GATCATCTG IGL_V_03- CTTTGGGACAGATGGCCAGGGGCTGGCAAGCCACGTTTGG SEQ ID 04_P_D10 GATCACCTG TGCTTTGGGACAGATGGCCAGNO: 6328 GATCACCTG IGL_V_03- CCAGGACAGGCAGCCATGA GGGCTGGCAAGCCACGTTTGGSEQ ID 06_P_D10 TCACCTG TGCCAGGACAGGCAGCCATGA NO: 6329 TCACCTG IGL_V_03-TGGGACAGAGGGCCAGGAT GGGCTGGCAAGCCACGTTTGG SEQ ID 07_P_D10 CACCTATGTGGGACAGAGGGCCAGGAT NO: 6330 CACCTA IGL_V_03- GGGACAGGCGGCCAGGATTGGGCTGGCAAGCCACGTTTGG SEQ ID 09_FP_D10 ACCTG TGGGGACAGGCGGCCAGGATT NO:6331 ACCTG IGL_V_03- CCAGGACAAACGGCCAGGA GGGCTGGCAAGCCACGTTTGG SEQ ID10_F_D10 TCACCTG TGCCAGGACAAACGGCCAGGA NO: 6332 TCACCTG IGL_V_03-CACAGCACAGATGGCCAGG GGGCTGGCAAGCCACGTTTGG SEQ ID 12_F_D10 ATCACCTGTGCACAGCACAGATGGCCAGG NO: 6333 ATCACCTG IGL_V_03- CCAGGACAGACAGCCAGGAGGGCTGGCAAGCCACGTTTGG SEQ ID 13_P_D10 TCAGCTG TGCCAGGACAGACAGCCAGGA NO:6334 TCAGCTG IGL_V_03- CCCCAGGACAGATGACCAG GGGCTGGCAAGCCACGTTTGG SEQ ID15_P_D10 GATCACCTG TGCCCCAGGACAGATGACCAG NO: 6335 GATCACCTG IGL_V_03-CCCTAGGACAGATGGCCAG GGGCTGGCAAGCCACGTTTGG SEQ ID 16_F_D10 GATCACCTGTGCCCTAGGACAGATGGCCAG NO: 6336 GATCACCTG IGL_V_03- GTGTCTGTGGACAGTCAGCGGGCTGGCAAGCCACGTTTGG SEQ ID 17_P_D10 AAGGGTAACCTG TGGTGTCTGTGGACAGTCAGCNO: 6337 AAGGGTAACCTG IGL_V_03- GGCCTTGGGACAGACAGTCGGGCTGGCAAGCCACGTTTGG SEQ ID 19_F_D10 AGGATCACATG TGGGCCTTGGGACAGACAGTCNO: 6338 AGGATCACATG IGL_V_03- CCCCAGGAAAGACGGCCAG GGGCTGGCAAGCCACGTTTGGSEQ ID 21_F_D10 GATTACCTG TGCCCCAGGAAAGACGGCCAG NO: 6339 GATTACCTGIGL_V_03- CCCAGGACAGAAAGCCAGG GGGCTGGCAAGCCACGTTTGG SEQ ID 22_FP_D10ATCACCTG TGCCCAGGACAGAAAGCCAGG NO: 6340 ATCACCTG IGL_V_03-CAGTAGCTCCAGGACAGAT GGGCTGGCAAGCCACGTTTGG SEQ ID 24_P_D10GACTAGGATCACCTG TGCAGTAGCTCCAGGACAGAT NO: 6341 GACTAGGATCACCTG IGL_V_03-CAGGACAGACGGCCAGGAT GGGCTGGCAAGCCACGTTTGG SEQ ID 25_F_D10 CACCTGTGCAGGACAGACGGCCAGGAT NO: 6342 CACCTG IGL_V_03- CCTGGGACAGTCAGCCAGGGGGCTGGCAAGCCACGTTTGG SEQ ID 26_P_D10 GTAACCTG TGCCTGGGACAGTCAGCCAGG NO:6343 GTAACCTG IGL_V_03- CGGGACAGACAGCCAGGAT GGGCTGGCAAGCCACGTTTGG SEQ ID27_F_D10 CACCTG TGCGGGACAGACAGCCAGGAT NO: 6344 CACCTG IGL_V_03-CCCAGGACAGACACCCAGG GGGCTGGCAAGCCACGTTTGG SEQ ID 29_P_D10 ATCACCTGTGCCCAGGACAGACACCCAGG NO: 6345 ATCACCTG IGL_V_03- CCCCATTACAGATGGCCAGGGGCTGGCAAGCCACGTTTGG SEQ ID 30_P_D10 GATCACCTG TGCCCCATTACAGATGGCCAGNO: 6346 GATCACCTG IGL_V_03- GCCTTGGGATAGACAGCCA GGGCTGGCAAGCCACGTTTGGSEQ ID 31_P_D10 GGATCACCTG TGGCCTTGGGATAGACAGCCA NO: 6347 GGATCACCTGIGL_V_03- CCTTGGGACAAATGGCCAG GGGCTGGCAAGCCACGTTTGG SEQ ID 32_O_D10GATCACCTG TGCCTTGGGACAAATGGCCAG NO: 6348 GATCACCTG IGL_V_04-CTGGGAGCCTCGATCAAGC GGGCTGGCAAGCCACGTTTGG SEQ ID 03_F_D10 TCACCTGTGCTGGGAGCCTCGATCAAGC NO: 6349 TCACCTG IGL_V_04- CCTGGGATCCTCGGTCAAGGGGCTGGCAAGCCACGTTTGG SEQ ID 60_F_D10 CTCACCTG TGCCTGGGATCCTCGGTCAAG NO:6350 CTCACCTG IGL_V_04- GGGAGCCTCGGTCAAGCTC GGGCTGGCAAGCCACGTTTGG SEQ ID69_F_D10 ACCTG TGGGGAGCCTCGGTCAAGCTC NO: 6351 ACCTG IGL_V_05-TCCTGGAGAATCCGCCAGA GGGCTGGCAAGCCACGTTTGG SEQ ID 37_F_D10 CTCACCTGTGTCCTGGAGCATCAGCCAGA NO: 6352 CTCACCTG IGL_V_05- TCTCCTGGAGCATCAGCCAGGGCTGGCAAGCCACGTTTGG SEQ ID 39_F_D10 GATTCACCTG TGTCTCCTGGAGCATCAGCCANO: 6353 GATTCACCTG IGL_V_05- TCCTGGAGCATCAGCCAGT GGGCTGGCAAGCCACGTTTGGSEQ ID 45_F_D10 CTCACCTG TGTCCTGGAGCATCAGCCAGT NO: 6354 CTCACCTGIGL_V_05- TCCTGGAGCATCAGCCAGA GGGCTGGCAAGCCACGTTTGG SEQ ID 48_O_D10CTCACCTG TGTCCTGGAGCATCAGCCAGA NO: 6355 CTCACCTG IGL_V_05-GCATCTTCTGGAGCATCAG GGGCTGGCAAGCCACGTTTGG SEQ ID 52_F_D10 TCAGACTCACCTGTGGCATCTTCTGGAGCATCAG NO: 6356 TCAGACTCACCTG IGL_V_06-TCCGGGGAAGACGGTAACC GGGCTGGCAAGCCACGTTTGG SEQ ID 57_F_D10 ATCTCCTGTGTCCGGGGAAGACGGTAACC NO: 6357 ATCTCCTG IGL_V_07- CCCAGGAGGGACAGTCACTGGGCTGGCAAGCCACGTTTGG SEQ ID 35_P_D10 CTCACCTA TGCCCAGGAGGGACAGTCACT NO:6358 CTCACCTA IGL_V_07- CCCAGGAGGGACAGTCACT GGGCTGGCAAGCCACGTTTGG SEQ ID43_F_D10 CTCACCTG TGCCCAGGAGGGACAGTCACT NO: 6359 CTCACCTG IGL_V_08-CCCCTGGAGGGACAGTCAC GGGCTGGCAAGCCACGTTTGG SEQ ID 61_F_D10 ACTCACTTGTGCCCCTGGAGGGACAGTCAC NO: 6360 ACTCACTTG IGL_V_09- TGGGAGCCTCGGTCACACTGGGCTGGCAAGCCACGTTTGG SEQ ID 49_F_D10 CACCTG TGTGGGAGCCTCGGTCACACT NO:6361 CACCTG IGL_V_10- CTTGAGACAGACCGCCACA GGGCTGGCAAGCCACGTTTGG SEQ ID54_F_D10 CTCACCTG TGCTTGAGACAGACCGCCACA NO: 6362 CTCACCTG IGK_J_01_F_D10TTCTACTCACGTTTGATTT CCGGGAGCTGCATGTGTCAGA SEQ ID CCACCTTGGTCCCGGTTCTACTCACGTTTGATTT NO: 6363 CCACCTTGGTCCC IGK_J_02_F_D10AAGTACTTACGTTTGATCT CCGGGAGCTGCATGTGTCAGA SEQ ID CCAGCTTGGTCCCGGAAGTACTTACGTTTGATCT NO: 6364 CCAGCTTGGTCCC IGK_J_03_F_D10ACAGATGTACTTACGTTTG CCGGGAGCTGCATGTGTCAGA SEQ ID ATATCCACTTTGGTCCCGGACAGATGTACTTACGTTTG NO: 6365 ATATCCACTTTGGTCCC IGK_J_04_F_D10CACTTACGTTTGATCTCCA CCGGGAGCTGCATGTGTCAGA SEQ ID CCTTGGTCCCGGCACTTACGTTTGATCTCCA NO: 6366 CCTTGGTCCC IGK_J_05_F_D10GAAAAATTACTTACGTTTA CCGGGAGCTGCATGTGTCAGA SEQ ID ATCTCCAGTCGTGTCCCGGGAAAAATTACTTACGTTTA NO: 6367 ATCTCCAGTCGTGTCCC IGL_J_01_F_D10CTTACCTAGGACGGTGACC CCGGGAGCTGCATGTGTCAGA SEQ ID TTGGTCCCGGCTTACCTAGGACGGTGACC NO: 6368 TTGGTCCC IGL_J_02_F_D10ACCTAGGACGGTCAGCTTG CCGGGAGCTGCATGTGTCAGA SEQ ID GTCCCGGACCTAGGACGGTCAGCTTG NO: 6369 GTCCC IGL_J_04_O_D10 AAGAAGAGACTCATCTAAACCGGGAGCTGCATGTGTCAGA SEQ ID ATGATCAGCTGGGTTCC GGAAGAAGAGACTCATCTAAA NO:6370 ATGATCAGCTGGGTTCC IGL_J_05_O_D10 ATCTAGGACGGTCAGCTCCCCGGGAGCTGCATGTGTCAGA SEQ ID GTCCC GGATCTAGGACGGTCAGCTCC NO: 6371 GTCCCIGL_J_06_F_D10 GAGGACGGTCACCTTGGTG CCGGGAGCTGCATGTGTCAGA SEQ ID CCGGGAGGACGGTCACCTTGGTG NO: 6372 CC IGL_J_07_F_D10 AGGACGGTCAGCTGGGTGCCCGGGAGCTGCATGTGTCAGA SEQ ID C GGAGGACGGTCAGCTGGGTGC NO: 6373 CIGK_J_del_F_D10 CTGCAGACTCATGAGGAGT CCGGGAGCTGCATGTGTCAGA SEQ ID CGCCCGGCTGCAGACTCATGAGGAGT NO: 6374 CGCCC

The invention claimed is:
 1. A method for determining non-uniformnucleic acid amplification potential among members of a set ofoligonucleotide primers that is capable of amplifying rearranged nucleicacid molecules encoding one or more adaptive immune receptors in abiological sample from a mammalian subject, the method comprising: (a)amplifying a composition comprising a plurality of synthetic templateoligonucleotides comprising sequences of rearranged nucleic acidmolecules encoding one or more adaptive immune receptors and one or moreadditional unique random polynucleotide sequences using a set ofoligonucleotide primers in a single multiplex PCR reaction to obtain aplurality of amplified synthetic template oligonucleotides; (b)sequencing said plurality of amplified synthetic templateoligonucleotides to determine, for each unique synthetic templateoligonucleotide comprising said plurality, (i) a synthetic templateoligonucleotide sequence and (ii) a frequency of occurrence of saidsynthetic template oligonucleotide sequence; and (c) comparing afrequency of occurrence of each of said synthetic templateoligonucleotide sequences to an expected distribution, wherein adeviation between said frequency of occurrence of said synthetictemplate oligonucleotide sequences and said expected distributionindicates a non-uniform nucleic acid amplification potential amongmembers of the set of oligonucleotide amplification primers.
 2. Themethod in claim 1, wherein said expected distribution is based ondetermining predetermined molar ratios of said plurality of synthetictemplate oligonucleotides comprising said composition.
 3. The method ofclaim 2, wherein said predetermined molar ratios are equimolar.
 4. Themethod in claim 1, wherein said expected distribution is determinedbased on quantifying frequencies of each unique synthetic templateoligonucleotide.
 5. The method of claim 1, wherein said expecteddistribution comprises a uniform amplification level for said pluralityof synthetic template oligonucleotides amplified by said set ofoligonucleotide primers.
 6. The method of claim 1, further comprisingadjusting the relative representation of the oligonucleotide primermember in the set of oligonucleotide amplification primers for eachmember of the set of oligonucleotide primers that exhibits non-uniformamplification potential relative to the expected distribution.
 7. Themethod of claim 6, wherein adjusting comprises: increasing the relativerepresentation of the member in the set of oligonucleotide primers,thereby correcting non-uniform nucleic acid amplification potentialamong members of the set of oligonucleotide primers, or decreasing therelative representation of the member in the set of oligonucleotideprimers, thereby correcting non-uniform nucleic acid amplificationpotential among members of the set of oligonucleotide primers.
 8. Themethod of claim 2, further comprising calculating a proportionatelyincreased or decreased frequency of occurrence of the amplified templatenucleic acid molecules for each member of the set of oligonucleotideamplification primers that exhibits non-uniform amplification potentialrelative to the expected distribution, thereby correcting fornon-uniform nucleic acid amplification potential among members of theset of oligonucleotide primers.
 9. The method of claim 1 wherein eachamplified synthetic template nucleic acid molecule is less than 1000,900, 800, 700, 600, 500, 400, 300, 200, 100, 90, 80 or 70 nucleotides inlength.
 10. The method of claim 1 wherein said set of oligonucleotideprimers does not include oligonucleotide primers that specificallyhybridize to a V-region pseudogene or orphon or to a J-region pseudogeneor orphon.
 11. The method of claim 1 wherein said plurality of synthetictemplate oligonucleotides each comprise an oligonucleotide sequence of ageneral formula:5′-U1-B1-V-B2-R-B3-J-B4-U2-3′  [I] wherein: (a) V is an oligonucleotidesequence comprising at least 20 and not more than 1000 contiguousnucleotides of an adaptive immune receptor variable (V) region encodinggene sequence, or the complement thereof, and each V comprising a uniqueV-region oligonucleotide sequence; (b) J is an oligonucleotide sequencecomprising at least 15 and not more than 600 contiguous nucleotides ofan adaptive immune receptor joining (J) region encoding gene sequence,or the complement thereof, and each J comprising a unique J-regionoligonucleotide sequence; (c) U1 is either nothing or comprises anoligonucleotide sequence that is selected from (i) a first universaladaptor oligonucleotide sequence and (ii) a first sequencingplatform-specific oligonucleotide sequence that is linked to andpositioned 5′ to a first universal adaptor oligonucleotide sequence; (d)U2 is either nothing or comprises an oligonucleotide sequence that isselected from (i) a second universal adaptor oligonucleotide sequence,and (ii) a second sequencing platform-specific oligonucleotide sequencethat is linked to and positioned 5′ to a second universal adaptoroligonucleotide sequence; (e) at least one of B1, B2, B3, and B4 ispresent and each of B1, B2, B3, and B4 comprises an oligonucleotidecomprising a barcode sequence of 3-25 contiguous nucleotides, thatuniquely identifies, as a paired combination, (i) the unique V-regionoligonucleotide sequence of (a) and (ii) the unique J-regionoligonucleotide sequence of (b); (f) R is either nothing or comprises arestriction enzyme recognition site that comprises an oligonucleotidesequence that is absent from (a)-(e); and (g) one or more unique randompolynucleotide sequences positioned between or within components offormula I.
 12. The method of claim 11 wherein the one or more uniquerandom polynucleotide sequences each comprises at least 2, 3, 4, 5, 6,7, 8, 9, 1,0 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, 50, 55,60, 70, 80, 90, 100, 200, 300, 400, 500 or more contiguous nucleotides.13. The method of claim 11 wherein the plurality of synthetic templateoligonucleotides comprises a number of at least a or at least b uniqueoligonucleotide sequences, whichever is larger, wherein a is the numberof unique adaptive immune receptor V region-encoding gene segments inthe subject and b is the number of unique adaptive immune receptor Jregion-encoding gene segments in the subject, and the compositioncomprises at least one synthetic template oligonucleotide for eachunique V-region oligonucleotide sequence and at least one synthetictemplate oligonucleotide for each unique J-region oligonucleotidesequence.
 14. The method of claim 13, wherein a ranges from 1 to anumber of maximum V gene segments in the mammalian genome of thesubject.
 15. The method of claim 13, wherein b ranges from 1 to a numberof maximum J gene segments in the mammalian genome of the subject. 16.The method of claim 13, wherein the plurality of templateoligonucleotides comprises at least (a ×b) unique oligonucleotidesequences, where a is the number of unique adaptive immune receptor Vregion-encoding gene segments in the mammalian subject and b is thenumber of unique adaptive immune receptor J region-encoding genesegments in the mammalian subject, and the composition comprises atleast one template oligonucleotide for every possible combination of a Vregion-encoding gene segment and a J region-encoding gene segment. 17.The method of claim 11, wherein J comprises an oligonucleotide sequencecomprising a constant region of the adaptive immune receptor J regionencoding gene sequence.
 18. The method of claim 1, wherein the one ormore adaptive immune receptors is selected from the group consisting ofTCRB, TCRG, TCRA, TCRD, IGH, IGK, and IGL.
 19. The method of claim 11,wherein the V oligonucleotide sequence of (a) encodes a TCRB, TCRG,TCRA, TCRD, IGH, IGK, or IGL receptor V-region polypeptide.
 20. Themethod of claim 11, wherein the J oligonucleotide sequence of (b)encodes a TCRB, TCRG, TCRA, TCRD, IGH, IGK, or IGL receptor J-regionpolypeptide.
 21. The method of claim 11, further comprising a stop codonsequence between V and B2.
 22. The method of claim 11, wherein V is anoligonucleotide sequence comprising at least 30 contiguous nucleotidesof the adaptive immune receptor V region encoding gene sequence, or thecomplement thereof.
 23. The method of claim 11, wherein V is anoligonucleotide sequence comprising not more than 900, 800, 700, 600 or500 contiguous nucleotides of an adaptive immune receptor V regionencoding gene sequence, or the complement thereof.
 24. The method ofclaim 11, wherein J is an oligonucleotide sequence comprising at least16 contiguous nucleotides of an adaptive immune receptor J regionencoding gene sequence, or the complement thereof.
 25. The method ofclaim 11, wherein J is an oligonucleotide sequence comprising not morethan 500 contiguous nucleotides of an adaptive immune receptor J regionencoding gene sequence, or the complement thereof.
 26. The method ofclaim 11, wherein each synthetic template oligonucleotide is less than1000, 900, 800, 700, 600, 500, 400, 300 or 200 nucleotides in length.27. The method of claim 1 wherein said one or more additional uniquerandom polynucleotide sequences each comprises at least 2 contiguousnucleotides.